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You may choose the
example sequence (Insert example)
provided in the form and
SCANMOT motifs (Insert motifs)
and search against
'nr'
database of sequences with
30% relaxation
for motifs and a
minimum of 2 motifs
to be present in the hits.
Step 1:
Use insert example link to paste sequence
Paste the query sequence in fasta format
(
insert example
)
Step 2:
Use insert example link to paste motifs
Paste Motifs Below
(In order which they appear in the query sequence)
(
Insert example
)
Order
Motifs
I
II
III
IV
V
VI
VII
VIII
IX
X
Click
here
to access a database of structural motifs for protein superfamilies.
Step 3: Choose "nr" form the drop down menu
Choose the database to search for motifs
nr
pdb
Swissprot-TrEMBL
SCOP
Custom Database
E.coli
A.thaliana
D.melanogaster
H.sapiens
M.musculus
S.cerevisiae
C.elegans
S.coelicolor
OR
Create Custom Database by Pasting the sequences below
(Note: The motifs will be searched only in these sequences)
Paste the sequences in fasta format
OR Upload Your file(database)-Sequences should be in fasta format
Wish to go for similar residue substitution?
eg:
[F]=[FYW], [V]=[VIL], [D]=[DE], [H]=[HKR], [N]=[NQ], [C]=[CST], A=[AG]
Yes
No
Step 4: Choose "30%" Relaxation filter from the drop down menu
Choose Relaxation filter for motif spacer length between query and hit
(click
here
for details)
30
5
10
15
20
25
30
35
40
45
50
%
Step :5 Choose "2" as minimum number of motifs
Choose the minimum number of motifs that should be present in the hits
Atleast
2
3
4
5
6
7
8
9
10
Motifs
Step :6 Press "Submit" button to iniate the process
Wish to go for
PSI-BLAST
search of your query and
mapping of the motifs by scanmot?
Click here
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PROSITE
|
PROFILE
|
PRINTS
|
FINDMOTIF
|
PFAM
|
BLOCKS
|
PDB
|
SCOP
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Contacts:
Sowdhamini.R -mini@ncbs.res.in
Saikat Chakrabarti -saikat@ncbs.res.in
Pugalenthi.G -pugal(at)ncbs.res.in
Prem Anand. A -prem@ncbs.res.in
Nitin Bhardwaj -nitinb@che.iitb.ac.in