PURE [Prediction of Unassigned REgions] Server:

PURE is a bioinformatics protocol to identify putative domains in the Unassigned Regions[UR]. PURE is now available as a web server. Users can submit the sequence of unassigned regions or linker regions to predict domains encoded in them.

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Input Methods:

NOTE : PURE Server users are encouraged to submit URs that are more than 70 amino acid residues long.

1. Upload Sequence File
2. Paste Sequence in FASTA Format

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Input Method 1 - Upload Sequence:

Enter your valid email:   PURE Results will be sent to this email address

Upload Your Sequence File here: [Click here for Example Run]

Switch-off TMAP/COIL Filter:

PSI-BLAST Database:   SwissProt NR PDB SCOP E.coli A.thaliana D.melanogaster H.sapiens M.musculus S.cerevisiae C.elegans

PSI-BLAST E-Value:   0.0001 0.001 0.01 0.1 1 10

CD-HIT Threshold Value:   0.9 0.8 0.7 0.6 0.4

HMMPFAM E-Value:   0.0001 0.001 0.01 0.1 1 10

HMMSEARCH Cut-off:   50 100 150 200

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Input Method 2 - Paste Sequence of Unassigned Regions in FASTA format:

Enter your valid email:   PURE Results will be sent to this email address

Enter Query Name of UR:

Enter Amino Acid Sequence Here:                    [Click here for Example Run]


Switch-off TMAP/COIL Filter:

PSI-BLAST Database:   SwissProt NR PDB SCOP E.coli A.thaliana D.melanogaster H.sapiens M.musculus S.cerevisiae C.elegans

PSI-BLAST E-Value:   0.0001 0.001 0.01 0.1 1 10

CD-HIT Threshold Value:   0.9 0.8 0.7 0.6 0.4

HMMPFAM E-Value:   0.0001 0.001 0.01 0.1 1 10

HMMSEARCH Cut-off:   50 100 150 200

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Contact:
Prof. R. Sowdhamini (mini@ncbs.res.in)
Dr. Bernard Offman
Chilamakuri Chandra Sekhar Reddy
K. Shameer