Pfam Domains mapped on to the structure: 2FRE

No.	Chain ID	Pfam ID	Pfam Description	Linkout - Pfam	Linkout - CDD
1	A	PF00881	Nitroreductase family	PF00881	PF00881

Gene Ontology Annotations: 2FRE

No.	GO ID	GO Description	Linkout - AmiGO
1	GO:0016491	oxidoreductase activity	GO:0016491

Conserved Domain Database Superfamily Annotations: 2FRE

No.	PDB ID	PSSM ID	CDD Accession	Superfamily Short Name	Linkout - CDD
1	2FRE	48385	cd02138	Nitroreductase_2 -	cl00514
2	2FRE	207085	cl00514	Nitro_FMN_reductase	superfamily - -

Structural Details of PDB entry 2FRE PDBid Chains Hinge Swapped Domain 2FRE A,B A:178-181,B:178-181 A:182-200,B:182-200 Swapped-domain interface residues and interactions: Chains Residues A 41, 44, 45, 56, 57, 58, 59, 60, 66, 147, 148, 182, 183, 184, 186, 189, 190, 191, 192, 193, 194, 195, 198, 199, 200, B 41, 44, 45, 56, 57, 58, 59, 66, 147, 148, 150, 182, 183, 184, 186, 189, 190, 191, 192, 193, 194, 195, Non-swapped-domain interface residues and interactions: Chains Residues A 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 20, 33, 34, 37, 38, 40, 42, 46, 47, 51, 53, 69, 94, 108, 109, 111, 112, 115, 116, 118, 119, 120, 124, 127, 136, 140, 146, 150, 153, 154, 156, 157, 181, B 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 20, 33, 34, 37, 38, 40, 42, 46, 47, 51, 53, 64, 94, 98, 108, 109, 111, 112, 115, 116, 118, 119, 120, 124, 127, 136, 140, 153, 154, 156, 157, 181,

Swapped domains are represented using trasperent spheres. Non-swapped part is represented using light color and cartoon representation. Hinge region is shown in yellow color.

Mutations in critical regions:

Chains	Hinge	Domain swapped interface	Non-swapped interface	Swapped Domain
A	No mutation	No mutation	No mutation	No mutation
B	No mutation	No mutation	No mutation	No mutation

HIDE output:

Homologues found through HIDE algorithm

JMOL Visualization:

2D-plot:

A:2FRE

B:2FRE

JOY Structural annotation for hinge hinge and swapped domain:

Hinge	Swapped domain

JOY output:

ali file:	2FRE.ali
atm file:	2FRE.atm
cof file:	2FRE.cof
hbd file:	2FRE.hbd
html file:	2FRE.html
pdb file:	2FRE.pdb
ps file:	2FRE.ps
psa file:	2FRE.psa
rtf file:	2FRE.rtf
sst file:	2FRE.sst
tem file:	2FRE.tem