Pfam Domains mapped on to the structure: 1GAF

No.	Chain ID	Pfam ID	Pfam Description	Linkout - Pfam	Linkout - CDD
1	L	PF13895	Immunoglobulin domain	PF13895	PF13895
2	L	PF13895	Immunoglobulin domain	PF13895	PF13895
3	L	PF13927	Immunoglobulin domain	PF13927	PF13927
4	H	PF13895	Immunoglobulin domain	PF13895	PF13895

Conserved Domain Database Superfamily Annotations: 1GAF

No.	PDB ID	PSSM ID	CDD Accession	Superfamily Short Name	Linkout - CDD
1	1GAF	143181	cd04980	IgV_L_kappa -	cl11960
2	1GAF	212623	cl11960	Ig	superfamily - -
3	1GAF	143323	cd07699	IgC_L -	cl11960
4	1GAF	212623	cl11960	Ig	superfamily - -
5	1GAF	212623	cl11960	Ig	superfamily - -

Structural Details of PDB entry 1GAF PDBid Chains Hinge Swapped Domain 1GAF H,L H:41-45,L:40-44 H:1-40,L:1-39 Swapped-domain interface residues and interactions: Chains Residues H 33, 35, 37, 39, L 1, 36, 38, Non-swapped-domain interface residues and interactions: Chains Residues H 44, 45, 47, 50, 59, 61, 62, 95, 100, 101, 102, 104, 123, 124, 125, 126, 129, 130, 136, 138, 142, 144, 165, 166, 167, 168, 170, 180, 182, 184, 215, L 42, 44, 46, 49, 55, 87, 89, 91, 94, 95, 96, 98, 100, 116, 118, 119, 121, 123, 124, 131, 133, 135, 137, 138, 160, 162, 163, 164, 167, 174, 176, 180, 209, 212, 214,

Swapped domains are represented using trasperent spheres. Non-swapped part is represented using light color and cartoon representation. Hinge region is shown in yellow color.

Mutations in critical regions:

Chains	Hinge	Domain swapped interface	Non-swapped interface	Swapped Domain
H	No mutation	No mutation	No mutation	No mutation
L	ASP(41)GLY, GLY(42)LYS, ILE(44)PRO,	LEU(36)TYR,	GLY(42)LYS, ILE(44)PRO, ARG(46)LEU, HIS(55)GLU, LEU(89)GLN, TYR(94)LEU, ARG(96)LEU,	LEU(15)VAL, GLU(17)ASP, SER(20)THR, LEU(21)ILE, ARG(24)GLN, GLU(28)ASP, GLY(31)ASN, GLY(34)ASN, LEU(36)TYR,

HIDE output:

Homologues found through HIDE algorithm

JMOL Visualization:

2D-plot:

H:1GAF

JOY Structural annotation for hinge hinge and swapped domain:

Hinge	Swapped domain

JOY output:

ali file:	1GAF.ali
atm file:	1GAF.atm
cof file:	1GAF.cof
hbd file:	1GAF.hbd
html file:	1GAF.html
pdb file:	1GAF.pdb
ps file:	1GAF.ps
psa file:	1GAF.psa
rtf file:	1GAF.rtf
sst file:	1GAF.sst
tem file:	1GAF.tem