HEADER COMPLEX (TRANSDUCER/TRANSDUCTION) COMPND MOL_ID: 1; COMPND 2 MOLECULE: TRANSDUCIN; COMPND 3 CHAIN: B, G; COMPND 4 FRAGMENT: LYS-C RESISTANT FRAGMENT, THE GAMMA SUBUNIT COMPND 5 CLEAVED AFTER RESIDUE 68; COMPND 6 SYNONYM: GT BETA-GAMMA; COMPND 7 MOL_ID: 2; COMPND 8 MOLECULE: PHOSDUCIN; COMPND 9 CHAIN: P; COMPND 10 SYNONYM: MEKA, PP33; COMPND 11 ENGINEERED: N-TERMINAL EXTENSION OF THE SEQUENCE COMPND 12 MGSSHHHHHHSSGLVPRGSH. EXPRESSED IN MINIMAL MEDIA, WITH COMPND 13 SELENOMETHIONINE REPLACING METHIONINE SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: BOS TAURUS; SOURCE 3 ORGANISM_COMMON: BOVINE; SOURCE 4 ORGAN: EYE; SOURCE 5 TISSUE: RETINA; SOURCE 6 CELLULAR_LOCATION: ROD OUTER SEGMENTS; SOURCE 7 OTHER_DETAILS: PURIFIED FROM BOVINE ROD OUTER SEGMENTS; SOURCE 8 MOL_ID: 2; SOURCE 9 ORGANISM_SCIENTIFIC: RATTUS NORVEGICUS; SOURCE 10 ORGANISM_COMMON: RAT; SOURCE 11 STRAIN: ZIVIC-MILLER SPRAGUE-DAWLEY; SOURCE 12 ATCC: 63147; SOURCE 13 ORGAN: PINEAL GLAND, RETINA; SOURCE 14 CELLULAR_LOCATION: CYTOSOLIC; SOURCE 15 GENE: PDC; SOURCE 16 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 17 EXPRESSION_SYSTEM_STRAIN: B834 (DE3); SOURCE 18 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 19 EXPRESSION_SYSTEM_VECTOR: PET15B; SOURCE 20 EXPRESSION_SYSTEM_PLASMID: PET15B/PHOSDUCIN; SOURCE 21 EXPRESSION_SYSTEM_GENE: RAT PDC RESOLUTION SOURCE : NSLS AUTHOR R.GAUDET,A.BOHM,P.B.SIGLER RESOLUTION : BRUNGER EXPDTA X-RAY DIFFRACTION JRNL AUTH R.GAUDET,A.BOHM,P.B.SIGLER JRNL TITL CRYSTAL STRUCTURE AT 2.4 ANGSTROMS RESOLUTION OF JRNL TITL 2 THE COMPLEX OF TRANSDUCIN BETAGAMMA AND ITS JRNL TITL 3 REGULATOR, PHOSDUCIN JRNL REF CELL(CAMBRIDGE,MASS.) V. 87 577 1996 JRNL REFN ASTM CELLB5 US ISSN 0092-8674 0998 RESOLUTION 2.4 ANGSTROMS.
Sequence in PIR format:
>P1;2trcP structureX:2trcP: 14 :P: 230 :P:phosducin:rattus norvegicus: 9.99: 9.99 EGQATHTGPKGVINDWRKFKLESEDGDSIPPSKKEILRQMSSPQSRDDKDSKER/SRK/SIQEYELIHQDKEDEG CLRKYRRQCQDHQKLSFGPRYGFVYELETGEQFLETIEKEQKVTTIVVNIYEDGVRGCDALNSSLECLAAEYPVK FCKIRASNTGAGDRFSSDVLPTLLVYKGGELISNFISVAEQFAEDFFAADVESFLNEYGLLPER*
View SSTRUC file for 2trcP
Rasmol View of 2trcP