HEADER HYDROLASE (GLUCANASE) COMPND 1,3-1,4-BETA-D-GLUCAN 4 GLUCANOHYDROLASE (E.C.3.2.1.73) COMPND 2 (BETA-GLUCANASE, LICHENASE) COMPLEXED WITH CALCIUM COMPND 3 (SYNCHROTRON X-RAY DIFFRACTION) RESOLUTION SOURCE 1,3-1,4-BETA-D-GLUCAN 4 GLUCANOHYDROLASE: HYBRID PROTEIN SOURCE 2 MOLECULE WITH RESIDUES 1 - 16 DERIVED FROM BACILLUS SOURCE 3 AMYLOLIQUEFACIENS BETA-GLUCANASE AND RESIDUES 17 - 214 SOURCE 4 DERIVED FROM THE HOMOLOGOUS BACILLUS MACERANS BETA- SOURCE 5 GLUCANASE; EXPRESSION SYSTEM: ESCHERICHIA COLI; SOURCE 6 PLASMID: PBR322 RESOLUTION D BEAM LINE: DESY (HAMBURG) X31 AUTHOR M.HAHN,T.KEITEL,U.HEINEMANN RESOLUTION TRONRUD,TEN EYCK,MATTHEWS EXPDTA SYNCHROTRON X-RAY DIFFRACTION JRNL AUTH M.HAHN,T.KEITEL,U.HEINEMANN JRNL TITL CRYSTAL AND MOLECULAR STRUCTURE AT 1.6 ANGSTROMS JRNL TITL 2 RESOLUTION OF THE HYBRID BACILLUS JRNL TITL 3 ENDO-1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE JRNL TITL 4 H(A16-M) JRNL REF TO BE PUBLISHED JRNL REFN 0353 JRNL TITL 2 RESOLUTION OF THE HYBRID BACILLUS RESOLUTION 1.6 ANGSTROMS.

Sequence in PIR format:

>P1;2ayh- structureX:2ayh-: 1 : : 214 : :1,3-1,4-beta-d-glucan 4 glucanohydrolase (e.c.3.2.1.73):1,3-1,4-beta-d-glucan 4 glucanohydrolase: hybrid protein: 9.99: 9.99 QTGGSFFEPFNSYNSGTWEKADGYSNGGVFNCTWRANNVNFTNDGKLKLGLTSSAYNKFDCAEYRSTNIYGYGLY EVSMKPAKNTGIVSSFFTYTGPAHGTQWDEIDIEFLGKDTTKVQFNYYTNGVGGHEKVISLGFDASKGFHTYAFD WQPGYIKWYVDGVLKHTATANIPSTPGKIMMNLWNGTGVDDWLGSYNGANPLYAEYDWVKYTSN*

View SSTRUC file for 2ayh-

Rasmol View of 2ayh-