HEADER    ISOMERASE                               
COMPND    MOL_ID: 1;                                                  
COMPND   2 MOLECULE: METHYLMALONYL-COA MUTASE;                        
COMPND   3 CHAIN: A, B, C, D;                                         
COMPND   4 EC: 5.4.99.2;                                              
COMPND   5 ENGINEERED: YES;                                           
COMPND   6 OTHER_DETAILS: CHAINS A AND C INCLUDE COENZYME B12,        
COMPND   7 DESULPHO-COA, AND A GLYCEROL IN THE ACTIVE SITE.  B12 IS   
COMPND   8 PRESENT LARGELY AS REDUCED COB(II)ALAMIN, OR B12R.         
SOURCE    MOL_ID: 1;                                                  
SOURCE   2 ORGANISM_SCIENTIFIC: PROPIONIBACTERIUM FREUDENREICHII      
SOURCE   3 SUBSP. SHERMANII;                                          
SOURCE   4 STRAIN: NCIB 9885;                                         
SOURCE   5 EXPRESSION_SYSTEM: K38 PGP1-2;                             
SOURCE   6 EXPRESSION_SYSTEM_PLASMID: PMEX1;                          
SOURCE   7 EXPRESSION_SYSTEM_GENE: MUTA, MUTB;                        
SOURCE   8 OTHER_DETAILS: THE 2 GENES MUTA (BETA CHAIN) AND MUTB      
SOURCE   9 (ALPHA CHAIN) ARE COEXPRESSED FROM THE SAME PLASMID        
RESOLUTION  SOURCE               : ESRF                       
AUTHOR    P.R.EVANS,F.MANCIA                                          
RESOLUTION     : MURSHUDOV                                    
EXPDTA    X-RAY DIFFRACTION                                           
JRNL        AUTH   F.MANCIA,N.H.KEEP,A.NAKAGAWA,P.F.LEADLAY,          
JRNL        AUTH 2 S.MCSWEENEY,B.RASMUSSEN,P.BOSECKE,O.DIAT,P.R.EVANS 
JRNL        TITL   HOW COENZYME B=12= RADICALS ARE GENERATED: THE     
JRNL        TITL 2 CRYSTAL STRUCTURE OF METHYLMALONYL-COA MUTASE AT   
JRNL        TITL 3 2 A RESOLUTION                                     
JRNL        REF    STRUCTURE (LONDON)            V.   4   339 1996    
JRNL        REFN   ASTM STRUE6  UK ISSN 0969-2126                 2005
RESOLUTION   2.0  ANGSTROMS.                                  



 Sequence in PIR format: 
>P1;1reqB
 structureX:1reqB:  20 :B: 638 :B:methylmalonyl-coa mutase:propionibacterium freudenreichii: 9.99: 9.99
 TLSLAGDFPKATEEQWEREVEKVLNRGRPPEKQLTFAECLKRLTVHTVDGIDIVPMYRPKDAPKKLGYPGVAPFT
 RGTTVRNGDMDAWDVRALHEDPDEKFTRKAILEGLERGVTSLLLRVDPDAIAPEHLDEVLSDVLLEMTKVEVFSR
 YDQGAAAEALVSVYERSDKPAKDLALNLGLDPIGFAALQGTEPDLTVLGDWVRRLAKFSPDSRAVTIDANIYHNA
 GAGDVAELAWALATGAEYVRALVEQGFTATEAFDTINFRVTATHDQFLTIARLRALREAWARIGEVFGVDEDKRG
 ARQNAITSWRELTREDPYVNILRGSIATFSASVGGAESITTLPFTQALGLPEDDFPLRIARNTGIVLAEEVNIGR
 VNDPAGGSYYVESLTRSLADAAWKEFQEVEKLGGMSKAVMTEHVTKVLDACNAERAKRLANRKQPITAVSEFPMI
 GARSIETKPFPAAPARKGLAWHRDSEVFEQLMDRSTSVSERPKVFLACLGTRRDFGGREGFSSPVWHIAGIDTPQ
 VEGGTTAEIVEAFKKSGAQVADLCSSAKVYAQQGLEVAKALKAAGAKALYLSGAFKEFGDDAAEAEKLIDGRLFM
 GMDVVDTLSSTLDILGVAK*




 View SSTRUC file for 1reqB 
 Rasmol View of  1reqB