HEADER PHOSPHOTRANSFERASE COMPND MOL_ID: 1; COMPND 2 MOLECULE: MANNOSE PERMEASE; COMPND 3 CHAIN: NULL; COMPND 4 FRAGMENT: IIA ==MAN== DOMAIN, RESIDUES 2 - 133, OF THE COMPND 5 IIAB ==MAN== SUBUNIT PLUS PHE-ALA-GLY AT THE CARBOXY COMPND 6 TERMINUS; COMPND 7 SYNONYM: MANNOSE TRANSPORTER, IIAB SUBUNIT; COMPND 8 EC: 2.7.1.69; COMPND 9 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI; SOURCE 3 STRAIN: K12/RP7029; SOURCE 4 EXPRESSION_SYSTEM: ESCHERICHIA COLI K12; SOURCE 5 EXPRESSION_SYSTEM_STRAIN: WA2127 AND D41; SOURCE 6 EXPRESSION_SYSTEM_PLASMID: PJFL1320; SOURCE 7 EXPRESSION_SYSTEM_GENE: MANX; SOURCE 8 OTHER_DETAILS: SUBCLONAL IIA COMPRISES RESIDUES 1 - 133 OF SOURCE 9 THE WILD-TYPE IIAB SEQUENCE PLUS PHE-ALA-GLY AT THE CARBOXY SOURCE 10 TERMINUS. THE INITIAL SE-MET WAS REMOVED BY PROTEOLYTIC SOURCE 11 CLEAVAGE. RESOLUTION SOURCE : SRS AUTHOR R.S.NUNN,B.ERNI,T.SCHIRMER RESOLUTION : BRUNGER EXPDTA X-RAY DIFFRACTION REVDAT 2 22-DEC-99 1PDO 1 JRNL JRNL AUTH R.S.NUNN,Z.MARKOVIC-HOUSLEY,J.-C.GENOVESIO-TAVERNE, JRNL AUTH 2 K.FLUKIGER,P.J.RIZKALLAH,J.N.JANSONIUS,T.SCHIRMER, JRNL AUTH 3 B.ERNI JRNL TITL STRUCTURE OF THE IIA DOMAIN OF THE MANNOSE JRNL TITL 2 TRANSPORTER FROM ESCHERICHIA COLI AT 1.7 A JRNL TITL 3 RESOLUTION JRNL REF J.MOL.BIOL. V. 259 502 1996 JRNL REFN ASTM JMOBAK UK ISSN 0022-2836 RESOLUTION 1.7 ANGSTROMS.

Sequence in PIR format:

>P1;1pdo- structureX:1pdo-: 2 : : 130 : :mannose permease:escherichia coli: 9.99: 9.99 TIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNA ASRIVVDKEHYEVIAGVNIPMLVETLMARDDDPSFDELVALAVETGREGVKALK*

View SSTRUC file for 1pdo-

Rasmol View of 1pdo-