HEADER TRANSCRIPTION COMPND MOL_ID: 1; COMPND 2 MOLECULE: SIGMA FACTOR SIGA; COMPND 3 CHAIN: A, B; COMPND 4 FRAGMENT: RESIDUES 92-332; COMPND 5 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: THERMUS AQUATICUS; SOURCE 3 ORGANISM_COMMON: BACTERIA; SOURCE 4 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 5 EXPRESSION_SYSTEM_COMMON: BACTERIA; SOURCE 6 EXPRESSION_SYSTEM_STRAIN: BL21(DE3); SOURCE 7 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 8 EXPRESSION_SYSTEM_PLASMID: PET21A RESOLUTION SOURCE : NSLS AUTHOR E.A.CAMPBELL,O.MUZZIN,M.CHLENOV,J.L.SUN,C.A.OLSON,O.WEINMAN, AUTHOR 2 M.L.TRESTER-ZEDLITZ,S.A.DARST RESOLUTION : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- EXPDTA X-RAY DIFFRACTION JRNL AUTH E.A.CAMPBELL,O.MUZZIN,M.CHLENOV,J.L.SUN,C.A.OLSON, JRNL AUTH 2 O.WEINMAN,M.L.TRESTER-ZEDLITZ,S.A.DARST JRNL TITL STRUCTURE OF THE BACTERIAL RNA POLYMERASE PROMOTER JRNL TITL 2 SPECIFICITY SIGMA SUBUNIT JRNL REF MOL. CELL V. 9 527 2002 JRNL REFN ASTM MOCEFL US ISSN 1097-2765 RESOLUTION 2.90 ANGSTROMS.

Sequence in PIR format:

>P1;1ku2A structureX:1ku2A: 93 :A: 332 :A:sigma factor siga:thermus aquaticus: 9.99: 9.99 SDPVRQYLHEIGQVPLLTLEEEIDLARKVEEGMEAIKKLSEATGLDQELIREVVRAKILGTARIQKIPGLKEKPD PKTVEEVDGKLKSLPKELKRYLHIAREGEAARQHLIEANLRLVVSIAKKYTGRGLSFLDLIQEGNQGLIRAVEKF EYKRRFKFSTYATWWIRQAINRAIADQARTIRIPVHMVETINKLSRTARQLQQELGREPSYEEIAEAMGPGWDAK RVEETLKIAQEPVSL*

View SSTRUC file for 1ku2A

Rasmol View of 1ku2A