HEADER HYDROLASE COMPND MOL_ID: 1; COMPND 2 MOLECULE: BETA LACTAMASE OXA-10; COMPND 3 CHAIN: A, B, C, D; COMPND 4 SYNONYM: BETA-LACTAMASE PSE-2; COMPND 5 EC: 3.5.2.6; COMPND 6 ENGINEERED: YES; COMPND 7 OTHER_DETAILS: RESIDUE 70 OF CHAINS A AND B, KCX ARE COMPND 8 CARBAMYLATED LYSINE. RESIDUE 70 OF CHAINS C AND D EXISTS COMPND 9 IN TWO ALTERNATE CONFORMATIONS: KCX, CARBAMYLATED LYSINE, COMPND 10 AND LYSINE. SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: PSEUDOMONAS AERUGINOSA; SOURCE 3 ORGANISM_COMMON: BACTERIA; SOURCE 4 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 5 EXPRESSION_SYSTEM_COMMON: BACTERIA; SOURCE 6 EXPRESSION_SYSTEM_STRAIN: BL21 DE3; SOURCE 7 EXPRESSION_SYSTEM_VECTOR_TYPE: PET24A RESOLUTION SOURCE : ESRF AUTHOR D.GOLEMI,L.MAVEYRAUD,S.VAKULENKO,J.P.SAMAMA,S.MOBASHERY RESOLUTION : MURSHUDOV,VAGIN,DODSON EXPDTA X-RAY DIFFRACTION REVDAT 2 19-DEC-01 1K55 1 JRNL JRNL AUTH D.GOLEMI,L.MAVEYRAUD,S.VAKULENKO,J.P.SAMAMA, JRNL AUTH 2 S.MOBASHERY JRNL TITL CRITICAL INVOLVEMENT OF A CARBAMYLATED LYSINE IN JRNL TITL 2 CATALYTIC FUNCTION OF CLASS D BETA-LACTAMASES JRNL REF PROC.NAT.ACAD.SCI.USA V. 98 14280 2001 JRNL REFN ASTM PNASA6 US ISSN 0027-8424 RESOLUTION 1.39 ANGSTROMS.
Sequence in PIR format:
>P1;1k55A structureX:1k55A: 21 :A: 265 :A:beta lactamase oxa-10:pseudomonas aeruginosa: 9.99: 9.99 SITENTSWNKEFSAEAVNGVFVLCKSSSKSCATNDLARASKEYLPASTFIPNAIIGLETGVIKNEHQVFKWDGKP RAMKQWERDLTLRGAIQVSAVPVFQQIAREVGEVRMQKYLKKFSYGNQNISGGIDKFWLEGQLRISAVNQVEFLE SLYLNKLSASKENQLIVKEALVTEAAPEYLVHSKTGFSGVGTESNPGVAWWVGWVEKETEVYFFAFNMDIDNESK LPLRKSIPTKIMESEGIIG*
View SSTRUC file for 1k55A
Rasmol View of 1k55A