HEADER TRANSCRIPTION/DNA COMPND MOL_ID: 1; COMPND 2 MOLECULE: TRANSCRIPTION REGULATOR NC2 ALPHA CHAIN; COMPND 3 CHAIN: A; COMPND 4 FRAGMENT: RESIDUES 1-77; COMPND 5 ENGINEERED: YES; COMPND 6 MOL_ID: 2; COMPND 7 MOLECULE: TRANSCRIPTION REGULATOR NC2 BETA CHAIN; COMPND 8 CHAIN: B; COMPND 9 SYNONYM: DR1 PROTEIN; COMPND 10 ENGINEERED: YES; COMPND 11 MOL_ID: 3; COMPND 12 MOLECULE: TATA-BOX-BINDING PROTEIN (TBP); COMPND 13 CHAIN: C; COMPND 14 FRAGMENT: RESIDUES 359-539; COMPND 15 SYNONYM: TRANSCRIPTION INITIATION FACTOR TFIID; COMPND 16 ENGINEERED: YES; COMPND 17 MOL_ID: 4; COMPND 18 MOLECULE: 5'- COMPND 19 D(*TP*TP*GP*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*GP*CP*TP*CP*C) COMPND 20 -3'; COMPND 21 CHAIN: D; COMPND 22 ENGINEERED: YES; COMPND 23 OTHER_DETAILS: 19 BASE-PAIR TATA-CONTAINING COMPND 24 OLIGONUCLEOTIDE TOP STRAND; COMPND 25 MOL_ID: 5; COMPND 26 MOLECULE: 5'- COMPND 27 D(*G*GP*AP*GP*CP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*CP*CP*AP*A)- COMPND 28 3'; COMPND 29 CHAIN: E; COMPND 30 ENGINEERED: YES; COMPND 31 OTHER_DETAILS: 19 BASE-PAIR TATA-CONTAINING COMPND 32 OLIGONUCLEOTIDE BOTTOM STRAND SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 3 ORGANISM_COMMON: HUMAN; SOURCE 4 GENE: NC2 ALPHA (DRAP1); SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 6 EXPRESSION_SYSTEM_COMMON: BACTERIA; SOURCE 7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3); SOURCE 8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 9 EXPRESSION_SYSTEM_PLASMID: PET11D; SOURCE 10 MOL_ID: 2; SOURCE 11 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 12 ORGANISM_COMMON: HUMAN; SOURCE 13 GENE: NC2 BETA (DR1); SOURCE 14 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 15 EXPRESSION_SYSTEM_COMMON: BACTERIA; SOURCE 16 EXPRESSION_SYSTEM_STRAIN: BL21(DE3); SOURCE 17 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 18 EXPRESSION_SYSTEM_PLASMID: PET28A; SOURCE 19 MOL_ID: 3; SOURCE 20 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 21 ORGANISM_COMMON: HUMAN; SOURCE 22 GENE: TBP; SOURCE 23 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 24 EXPRESSION_SYSTEM_COMMON: BACTERIA; SOURCE 25 EXPRESSION_SYSTEM_STRAIN: BL21(DE3); SOURCE 26 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 27 EXPRESSION_SYSTEM_PLASMID: PET15B; SOURCE 28 MOL_ID: 4; SOURCE 29 SYNTHETIC: YES; SOURCE 30 MOL_ID: 5; SOURCE 31 SYNTHETIC: YES RESOLUTION SOURCE : APS AUTHOR K.KAMADA,F.SHU,H.CHEN,S.MALIK,G.STELZER,R.G.ROEDER, AUTHOR 2 M.MEISTERERNST,S.K.BURLEY RESOLUTION : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- EXPDTA X-RAY DIFFRACTION JRNL AUTH K.KAMADA,F.SHU,H.CHEN,S.MALIK,G.STELZER,R.G.ROEDER, JRNL AUTH 2 M.MEISTERERNST,S.K.BURLEY JRNL TITL CRYSTAL STRUCTURE OF NEGATIVE COFACTOR 2 JRNL TITL 2 RECOGNIZING THE TBP-DNA TRANSCRIPTION COMPLEX JRNL REF CELL (CAMBRIDGE,MASS.) V. 106 71 2001 JRNL REFN ASTM CELLB5 US ISSN 0092-8674 RESOLUTION 2.62 ANGSTROMS.
Sequence in PIR format:
>P1;1jfiA structureX:1jfiA: 10 :A: 75 :A:5'-:homo sapiens: 9.99: 9.99 ARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSR/TMTTSHLKQCIE*
View SSTRUC file for 1jfiA
Rasmol View of 1jfiA