HEADER    LIGASE                                  
REVDAT   2   30-NOV-99 1IOW    1       JRNL   HEADER                  
COMPND    MOL_ID: 1;                                                  
COMPND   2 MOLECULE: D-ALA\:D-ALA LIGASE;                             
COMPND   3 CHAIN: NULL;                                               
COMPND   4 SYNONYM: DD-LIGASE, DDLB;                                  
COMPND   5 EC: 6.3.2.4;                                               
COMPND   6 ENGINEERED: YES;                                           
COMPND   7 MUTATION: Y216F                                            
SOURCE    MOL_ID: 1;                                                  
SOURCE   2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                     
SOURCE   3 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                       
SOURCE   4 EXPRESSION_SYSTEM_STRAIN: W3110;                           
SOURCE   5 EXPRESSION_SYSTEM_PLASMID: PTB2;                           
SOURCE   6 EXPRESSION_SYSTEM_GENE: DDLB;                              
SOURCE   7 OTHER_DETAILS: ENZYME PROVIDED BY C.T. WALSH, HARVARD MED  
SOURCE   8 SCHOOL.  SEE SHI AND WALSH, BIOCHEM. 34, P. 2768, 1995.    
RESOLUTION  SOURCE               : NULL                       
AUTHOR    J.R.KNOX,P.C.MOEWS,C.FAN                                    
RESOLUTION     : BRUNGER                                      
EXPDTA    X-RAY DIFFRACTION                                           
REVDAT   2   30-NOV-99 1IOW    1       JRNL   HEADER                  
JRNL        AUTH   C.FAN,I.-S.PARK,C.T.WALSH,J.R.KNOX                 
JRNL        TITL   D-ALANINE:D-ALANINE LIGASE: PHOSPHONATE AND        
JRNL        TITL 2 PHOSPHINATE INTERMEDIATES WITH WILD-TYPE AND THE   
JRNL        TITL 3 Y216F MUTANT                                       
JRNL        REF    BIOCHEMISTRY                  V.  36  2531 1997    
JRNL        REFN   ASTM BICHAW  US ISSN 0006-2960                     
RESOLUTION   1.9  ANGSTROMS.                                  



 Sequence in PIR format: 
>P1;1iow-
 structureX:1iow-:   1 : : 306 : :d-ala\:d-ala ligase:escherichia coli: 9.99: 9.99
 MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKEVDVTQLKSMGFQKVFIALHGRGGEDGTLQGM
 LELMGLPYTGSGVMASALSMDKLRSKLLWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGS
 SVGMSKVVAENALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKFLSDETQYFC
 PAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQLVVR
 ILELAD*




 View SSTRUC file for 1iow- 
 Rasmol View of  1iow-