Secondary structure calculation program - copyright by David Keith Smith, 1989 1i16-.pdb 1I16 CYTOKINE MOL_ID: 1; MOL_ID: 1; Sequence length - 130 A A K K hydrogen bonding Ooi's strk chain/ l amino u & S structure bridge dihedral angles donor acceptor donor acceptor N N num seq.no t acids t S + patterns partners phi psi omega alpha kappa tco to/energy fr/energy to/energy fr/energy 8 14 1 1 MET M 0 0 999.9 90.9 179.9 999.9 999.9 999.9 0 0.0 0 0.0 0 0.0 0 0.0 4 7 2 2 PRO P - 0 0 -79.0 -61.8 179.7 -1.3 999.9 15.8 0 0.0 0 0.0 0 0.0 0 0.0 3 6 3 3 ASP D S S S- 0 0 -117.7 -168.9 -180.0 -57.3 101.4 118.8 0 0.0 0 0.0 0 0.0 0 0.0 4 11 4 4 LEU L S S S- 0 0 -58.9 -176.3 -179.9 -74.1 75.1 85.2 0 0.0 0 0.0 0 0.0 0 0.0 6 14 5 5 ASN N + 0 0 -82.3 153.2 -180.0 140.0 67.1 120.6 0 0.0 0 0.0 0 0.0 0 0.0 7 13 6 6 SER S + 0 0 -164.7 -158.7 -180.0 82.9 31.5 147.7 0 0.0 0 0.0 0 0.0 0 0.0 8 13 7 7 SER S S S S+ 0 0 47.9 47.2 180.0 56.2 98.2 21.9 0 0.0 0 0.0 0 0.0 0 0.0 5 11 8 8 THR T S S S- 0 0 -175.7 -165.3 -180.0 -10.3 104.5 161.1 0 0.0 0 0.0 0 0.0 0 0.0 6 10 9 9 ASP D S S S- 0 0 -49.9 121.8 180.0 -107.8 78.7 104.2 0 0.0 11 -1.4 0 0.0 0 0.0 5 9 10 10 SER S S S S+ 0 0 -55.1 90.7 179.8 131.5 75.1 106.9 0 0.0 0 0.0 0 0.0 0 0.0 6 10 11 11 ALA A + 0 0 -145.7 71.9 -179.9 134.2 17.4 119.2 9 -1.4 0 0.0 0 0.0 0 0.0 6 12 12 12 ALA A - 0 0 -126.1 121.4 -179.9 -116.3 56.9 164.8 0 0.0 0 0.0 0 0.0 0 0.0 7 14 13 13 SER S + 0 0 -52.0 136.1 179.9 166.7 40.7 103.1 0 0.0 0 0.0 0 0.0 0 0.0 4 13 14 14 ALA A + 0 0 -118.0 -71.4 179.6 151.1 7.8 48.2 0 0.0 0 0.0 0 0.0 0 0.0 4 12 15 15 SER S - 0 0 54.5 169.6 179.8 -110.7 42.5 77.9 0 0.0 0 0.0 0 0.0 0 0.0 4 11 16 16 ALA A + 0 0 -126.0 -178.9 -179.8 177.8 32.2 133.6 0 0.0 0 0.0 0 0.0 0 0.0 5 10 17 17 ALA A + 0 0 -168.8 179.7 179.9 44.0 53.3 166.4 0 0.0 19 -2.8 0 0.0 0 0.0 5 9 18 18 SER S S S S+ 0 0 69.9 -74.8 -180.0 59.9 106.9 111.8 0 0.0 0 0.0 0 0.0 0 0.0 4 8 19 19 ASP D S S S- 0 0 -85.5 143.4 179.9 -168.9 71.6 128.8 17 -2.8 0 0.0 0 0.0 0 0.0 5 9 20 20 VAL V - 0 0 -133.5 141.9 180.0 -179.1 6.1 171.1 0 0.0 0 0.0 0 0.0 0 0.0 5 13 21 21 SER S + 0 0 -126.8 -175.5 179.9 66.2 48.4 131.5 0 0.0 0 0.0 0 0.0 0 0.0 4 11 22 22 VAL V S S S- 0 0 64.2 84.1 -179.9 -114.6 83.5 14.5 0 0.0 0 0.0 0 0.0 0 0.0 4 13 23 23 GLU E - 0 0 -43.6 156.2 179.9 -98.4 37.8 90.0 0 0.0 0 0.0 0 0.0 0 0.0 4 13 24 24 SER S - 0 0 -67.5 -172.5 -179.8 -116.9 39.9 87.0 0 0.0 0 0.0 0 0.0 0 0.0 6 18 25 25 THR T S S S+ 0 0 -128.5 161.7 179.8 12.9 83.9 152.1 0 0.0 0 0.0 0 0.0 0 0.0 5 17 26 26 ALA A S S S- 0 0 44.5 31.0 -179.9 -69.1 127.8 33.4 0 0.0 118 -2.0 0 0.0 0 0.0 7 17 27 27 GLU E S e S- 0 0 52.9 92.5 180.0 -174.2 73.5 21.2 0 0.0 118 -0.7 0 0.0 0 0.0 8 21 28 28 ALA A E E AA + 117 0 -121.3 132.3 -179.9 172.0 8.5 164.4 0 0.0 0 0.0 0 0.0 0 0.0 10 29 29 29 THR T E E AA - 116 0 -140.4 142.5 179.8 -144.5 19.6 176.4 116 -1.8 116 -2.0 0 0.0 0 0.0 7 31 30 30 VAL V E E AA - 115 0 -105.0 151.4 179.7 -162.3 5.2 140.6 0 0.0 0 0.0 0 0.0 0 0.0 8 39 31 31 CYS C E E AA - 114 0 -134.2 87.8 179.7 -167.7 4.6 136.9 114 -3.1 114 -0.8 0 0.0 33 -0.5 8 39 32 32 THR T E E AA + 113 0 -76.7 121.2 -178.8 155.4 25.6 128.0 0 0.0 0 0.0 0 0.0 0 0.0 8 33 33 33 VAL V E E AA - 112 0 -142.5 164.2 178.7 -122.8 38.4 161.9 112 -2.1 112 -5.0 31 -0.5 0 0.0 8 38 34 34 THR T E E AA - 111 0 -113.8 131.7 176.9 -167.1 20.8 157.5 0 0.0 0 0.0 0 0.0 0 0.0 10 40 35 35 LEU L E E AA - 110 0 -100.9 178.7 179.7 -109.2 28.2 117.6 110 -4.6 110 -3.3 0 0.0 37 -0.6 14 48 36 36 GLU E E E AA - 109 0 -114.7 115.8 -179.6 -145.0 19.3 156.9 0 0.0 38 -1.3 0 0.0 0 0.0 11 41 37 37 LYS K e + 0 0 -79.5 98.0 -179.9 96.5 68.9 127.4 108 -3.5 0 0.0 35 -0.6 0 0.0 13 40 38 38 MET M + 0 0 -151.1 -32.2 -179.5 66.3 64.1 65.1 36 -1.3 0 0.0 0 0.0 0 0.0 8 29 39 39 SER S S S S- 0 0 -85.2 19.7 179.5 -99.9 115.3 77.2 0 0.0 0 0.0 0 0.0 0 0.0 7 28 40 40 ALA A S S S+ 0 0 65.0 54.2 179.7 28.3 114.1 11.7 0 0.0 0 0.0 0 0.0 0 0.0 7 32 41 41 GLY G S t > TS- 0 0 134.5 29.1 -179.9 -93.9 115.5 57.0 0 0.0 44 -3.6 0 0.0 0 0.0 10 44 42 42 LEU L T T 3 TS- 0 0 40.0 25.6 179.1 -80.4 72.9 39.0 0 0.0 0 0.0 0 0.0 0 0.0 12 51 43 43 GLY G T e 3 TS+ 0 0 61.6 9.1 179.6 95.5 114.9 50.1 0 0.0 65 -3.7 0 0.0 0 0.0 11 43 44 44 PHE F E E BB < T - 64 0 -132.0 153.3 -179.5 -159.7 57.1 161.3 41 -3.6 0 0.0 0 0.0 0 0.0 10 56 45 45 SER S E E BB - 63 0 -130.6 144.2 -178.4 -133.6 15.0 166.4 63 -0.5 62 -2.0 0 0.0 63 -0.6 11 57 46 46 LEU L E E BB - 61 0 -103.0 135.7 177.8 -178.3 21.5 146.3 0 0.0 0 0.0 0 0.0 0 0.0 12 51 47 47 GLU E E E BB + 60 0 -128.2 149.3 179.9 107.2 28.0 161.7 60 -4.9 60 -3.0 0 0.0 0 0.0 9 41 48 48 GLY G - 0 0 -177.9 -119.9 179.4 -88.4 63.1 130.0 0 0.0 0 0.0 0 0.0 0 0.0 9 37 49 49 GLY G - 0 0 176.6 102.6 178.9 -133.2 24.0 114.0 0 0.0 0 0.0 0 0.0 0 0.0 10 32 50 50 LYS K - 0 0 -51.6 169.2 -179.9 -173.8 27.1 90.2 0 0.0 0 0.0 0 0.0 0 0.0 11 29 51 51 GLY G - 0 0 -133.8 -108.2 -180.0 -92.3 34.7 82.7 0 0.0 0 0.0 0 0.0 0 0.0 9 21 52 52 SER S - 0 0 -176.3 45.0 -179.7 -78.1 65.6 86.1 0 0.0 0 0.0 0 0.0 0 0.0 6 16 53 53 LEU L S S S+ 0 0 56.5 24.3 179.9 78.6 125.9 36.2 0 0.0 0 0.0 0 0.0 0 0.0 5 15 54 54 HIS H S S S+ 0 0 -142.1 5.9 179.8 16.6 100.4 73.3 0 0.0 0 0.0 0 0.0 0 0.0 5 13 55 55 GLY G S S S- 0 0 -142.8 -102.9 179.8 -98.2 89.7 86.9 0 0.0 0 0.0 0 0.0 0 0.0 6 17 56 56 ASP D - 0 0 165.3 120.3 179.7 -155.9 13.0 118.9 0 0.0 0 0.0 0 0.0 0 0.0 10 24 57 57 LYS K - 0 0 -112.7 85.3 -180.0 -176.4 13.2 137.6 0 0.0 59 -2.0 0 0.0 0 0.0 12 33 58 58 PRO P - 0 0 -79.0 75.5 -179.9 -151.0 20.2 121.1 0 0.0 0 0.0 0 0.0 0 0.0 12 43 59 59 LEU L - 0 0 -43.3 152.8 -178.7 -169.6 18.7 92.1 57 -2.0 0 0.0 0 0.0 0 0.0 15 46 60 60 THR T E E BB - 47 0 -146.4 159.0 179.2 -94.0 28.9 169.4 47 -3.0 47 -4.9 0 0.0 0 0.0 12 48 61 61 ILE I E E BB + 46 0 -79.1 125.8 179.8 157.2 48.2 128.2 80 -1.1 79 -2.8 0 0.0 0 0.0 15 47 62 62 ASN N E E B* + 0 0 -113.1 -49.8 -178.5 37.6 64.1 40.6 45 -2.0 64 -0.8 0 0.0 0 0.0 10 30 63 63 ARG R E E BB + 45 0 -111.2 93.7 178.5 178.6 68.3 140.3 45 -0.6 45 -0.5 0 0.0 0 0.0 8 28 64 64 ILE I E E BB - 44 0 -91.9 144.1 -177.9 -149.4 14.7 132.9 62 -0.8 0 0.0 0 0.0 0 0.0 10 34 65 65 PHE F e - 0 0 -108.1 170.8 -179.9 -113.6 15.4 127.9 43 -3.7 67 -0.7 0 0.0 0 0.0 6 27 66 66 LYS K S S S+ 0 0 -108.4 111.5 179.9 78.5 82.6 152.5 0 0.0 0 0.0 0 0.0 0 0.0 5 27 67 67 GLY G S S S+ 0 0 160.8 38.4 -179.8 33.2 87.3 71.9 65 -0.7 0 0.0 0 0.0 0 0.0 7 24 68 68 ALA A + 0 0 -177.8 -164.9 180.0 80.7 68.1 162.2 0 0.0 0 0.0 0 0.0 0 0.0 9 33 69 69 ALA A + 0 0 48.9 41.6 180.0 121.5 64.5 24.9 0 0.0 0 0.0 0 0.0 0 0.0 11 34 70 70 SER S + 0 0 -117.7 15.8 -179.8 73.6 51.5 79.3 0 0.0 0 0.0 0 0.0 0 0.0 8 26 71 71 GLU E S S S+ 0 0 -109.5 6.1 179.8 11.7 114.1 70.1 0 0.0 0 0.0 0 0.0 0 0.0 5 21 72 72 GLN Q S S S- 0 0 -179.0 71.8 179.7 -75.6 112.3 98.6 0 0.0 0 0.0 0 0.0 0 0.0 6 20 73 73 SER S S S S- 0 0 56.0 -170.7 -179.9 -26.7 94.4 90.7 0 0.0 75 -0.8 0 0.0 0 0.0 6 18 74 74 GLU E - 0 0 -76.4 110.8 180.0 -176.5 58.6 127.9 0 0.0 0 0.0 0 0.0 0 0.0 7 31 75 75 THR T + 0 0 -94.9 26.7 180.0 71.0 61.8 85.5 73 -0.8 0 0.0 0 0.0 0 0.0 10 42 76 76 VAL V - 0 0 -137.4 162.4 179.5 -165.7 58.2 158.8 0 0.0 0 0.0 0 0.0 0 0.0 6 50 77 77 GLN Q t > T - 0 0 -151.3 139.6 -179.6 -119.4 24.0 170.9 0 0.0 80 -0.9 0 0.0 0 0.0 7 48 78 78 PRO P T T 3 TS+ 0 0 -80.5 132.8 179.0 3.2 97.4 127.6 0 0.0 0 0.0 0 0.0 0 0.0 9 38 79 79 GLY G T T 3 TS+ 0 0 69.5 12.6 179.9 155.6 90.1 47.9 61 -2.8 0 0.0 0 0.0 0 0.0 7 36 80 80 ASP D t < T - 0 0 -71.6 140.3 180.0 -125.4 51.0 119.6 77 -0.9 82 -2.1 0 0.0 61 -1.1 10 44 81 81 GLU E e - 0 0 -83.1 64.9 179.8 -170.4 33.8 111.2 0 0.0 115 -0.6 0 0.0 0 0.0 11 52 82 82 ILE I E E AC - 114 0 -55.2 123.1 -179.7 -170.1 7.1 109.6 80 -2.1 0 0.0 0 0.0 0 0.0 14 56 83 83 LEU L E E A* + 0 0 -86.7 -42.2 -179.6 8.4 66.5 25.4 113 -2.5 90 -2.6 0 0.0 91 -0.7 13 52 84 84 GLN Q E E ACA - 113 89 -139.6 146.9 -179.8 -150.9 65.6 173.1 113 -0.7 113 -1.2 0 0.0 0 0.0 12 51 85 85 LEU L - 0 0 -99.6 -165.8 179.0 -69.6 55.5 103.0 88 -3.5 0 0.0 0 0.0 0 0.0 13 58 86 86 GLY G S S S+ 0 0 -62.6 -11.0 -179.8 1.0 133.9 46.0 0 0.0 0 0.0 0 0.0 0 0.0 12 45 87 87 GLY G S S S+ 0 0 -165.3 32.9 177.3 70.1 120.0 78.8 0 0.0 0 0.0 0 0.0 0 0.0 10 40 88 88 THR T - 0 0 -155.2 133.9 179.0 -150.3 55.4 162.1 0 0.0 85 -3.5 0 0.0 90 -0.7 11 45 89 89 ALA A B B A > T - 84 0 -108.6 108.5 -179.2 -162.8 4.7 150.4 0 0.0 92 -1.1 0 0.0 0 0.0 10 43 90 90 MET M G G > TS+ 0 0 -52.5 -57.3 -177.5 65.4 86.0 16.2 83 -2.6 93 -2.3 88 -0.7 0 0.0 15 45 91 91 GLN Q G G 3 TS+ 0 0 -41.1 -32.1 177.8 35.3 112.7 34.9 83 -0.7 0 0.0 0 0.0 0 0.0 11 40 92 92 GLY G G G < TS+ 0 0 -115.6 49.5 -177.9 67.8 116.6 107.4 89 -1.1 0 0.0 0 0.0 0 0.0 5 33 93 93 LEU L S g < TS- 0 0 -161.3 150.6 178.7 -118.1 76.9 169.5 90 -2.3 0 0.0 0 0.0 0 0.0 10 37 94 94 THR T h > T - 0 0 -80.6 -176.8 -179.8 -97.8 43.0 99.5 0 0.0 98 -1.4 0 0.0 0 0.0 12 35 95 95 ARG R H H > TS+ 0 0 -70.7 -56.1 -179.9 31.6 122.2 9.2 0 0.0 99 -2.2 0 0.0 0 0.0 14 39 96 96 PHE F H H > TS+ 0 0 -65.5 -53.8 179.9 50.3 121.2 10.9 0 0.0 100 -1.3 0 0.0 0 0.0 6 33 97 97 GLU E H H > TS+ 0 0 -51.3 -40.5 -179.8 52.2 111.6 24.6 0 0.0 101 -1.7 0 0.0 0 0.0 8 33 98 98 ALA A H H X > TS+ 0 0 -61.2 -64.2 -179.2 43.4 110.1 4.9 94 -1.4 102 -1.2 0 0.0 101 -0.6 13 43 99 99 TRP W H H X 3 TS+ 0 0 -58.4 -9.5 -180.0 64.5 110.1 48.7 95 -2.2 103 -1.3 0 0.0 0 0.0 11 49 100 100 ASN N H H X 3 TS+ 0 0 -82.2 -46.5 -179.4 52.6 94.4 21.6 96 -1.3 104 -1.9 0 0.0 0 0.0 8 37 101 101 ILE I H H < < TS+ 0 0 -56.8 -40.0 179.3 51.6 110.3 22.2 97 -1.7 0 0.0 98 -0.6 0 0.0 10 35 102 102 ILE I H H < > TS+ 0 0 -58.2 -64.2 -179.7 50.7 106.5 4.3 98 -1.2 105 -4.6 0 0.0 0 0.0 12 47 103 103 LYS K H H < 3 TS+ 0 0 -41.9 -36.5 -178.7 51.4 111.4 32.1 99 -1.3 0 0.0 0 0.0 0 0.0 8 37 104 104 ALA A T h < 3 TS+ 0 0 -83.0 -1.8 -179.6 107.2 90.9 59.9 100 -1.9 0 0.0 0 0.0 0 0.0 6 29 105 105 LEU L S t < TS- 0 0 -68.1 176.4 179.7 -93.5 78.0 96.5 102 -4.6 0 0.0 0 0.0 0 0.0 9 33 106 106 PRO P - 0 0 -82.6 177.7 -179.6 -91.2 43.3 100.7 0 0.0 108 -1.1 0 0.0 0 0.0 5 25 107 107 ASP D S S S+ 0 0 -96.3 92.6 179.7 70.8 89.2 136.1 0 0.0 0 0.0 0 0.0 0 0.0 7 24 108 108 GLY G S e S- 0 0 -166.0 -150.8 179.8 -18.3 92.7 142.4 106 -1.1 37 -3.5 0 0.0 110 -0.9 8 28 109 109 PRO P E E AA - 36 0 -77.4 104.9 179.0 -159.4 57.0 129.3 0 0.0 111 -0.5 0 0.0 0 0.0 9 36 110 110 VAL V E E AA - 35 0 -87.1 125.3 -178.3 -137.6 13.2 137.4 35 -3.3 35 -4.6 108 -0.9 112 -0.5 12 46 111 111 THR T E E AA - 34 0 -88.6 127.8 179.4 -168.2 20.2 135.7 109 -0.5 0 0.0 0 0.0 0 0.0 11 44 112 112 ILE I E E AA - 33 0 -110.5 152.8 178.7 -164.0 7.6 145.0 33 -5.0 33 -2.1 110 -0.5 0 0.0 14 55 113 113 VAL V E E AAC + 32 84 -138.8 108.2 -178.3 171.8 14.9 154.8 84 -1.2 83 -2.5 0 0.0 84 -0.7 12 44 114 114 ILE I E E AAC - 31 82 -121.3 141.7 178.6 -164.0 18.0 160.1 31 -0.8 31 -3.1 0 0.0 116 -0.6 12 48 115 115 ARG R E E AA - 30 0 -123.1 103.4 -179.2 -162.7 9.4 154.7 81 -0.6 117 -0.7 0 0.0 0 0.0 12 41 116 116 ARG R E E AA + 29 0 -88.5 117.7 -179.7 173.0 18.5 138.6 29 -2.0 29 -1.8 114 -0.6 0 0.0 10 36 117 117 LYS K E E AA + 28 0 -120.6 164.2 -179.7 32.7 38.1 143.5 115 -0.7 0 0.0 0 0.0 0 0.0 9 29 118 118 SER S S e S- 0 0 80.9 175.4 -179.7 -34.1 105.1 97.6 26 -2.0 0 0.0 27 -0.7 0 0.0 8 20 119 119 LEU L S S S+ 0 0 -55.2 -176.3 -180.0 75.6 105.3 82.4 0 0.0 0 0.0 0 0.0 0 0.0 6 20 120 120 GLN Q + 0 0 74.7 47.0 179.9 122.3 68.9 19.7 0 0.0 0 0.0 0 0.0 0 0.0 4 21 121 121 SER S + 0 0 -144.5 120.2 179.9 166.3 27.5 159.7 0 0.0 0 0.0 0 0.0 0 0.0 7 26 122 122 LYS K + 0 0 -134.5 81.8 -180.0 87.8 49.6 131.7 0 0.0 0 0.0 0 0.0 0 0.0 4 22 123 123 GLU E + 0 0 -165.1 38.0 -179.8 143.3 31.4 87.8 0 0.0 125 -1.9 0 0.0 0 0.0 5 27 124 124 THR T S S S- 0 0 -81.0 52.2 -180.0 -84.7 84.4 102.9 0 0.0 0 0.0 0 0.0 0 0.0 4 23 125 125 THR T + 0 0 45.3 87.6 -179.9 167.7 65.8 22.4 123 -1.9 0 0.0 0 0.0 0 0.0 6 14 126 126 ALA A + 0 0 -114.8 16.1 180.0 97.0 47.6 79.0 0 0.0 0 0.0 0 0.0 0 0.0 6 9 127 127 ALA A S S S- 0 0 -98.6 49.3 -180.0 -1.3 105.3 105.4 0 0.0 0 0.0 0 0.0 0 0.0 4 8 128 128 GLY G S S S+ 0 0 133.2 75.6 180.0 177.1 75.1 62.8 0 0.0 0 0.0 0 0.0 0 0.0 5 7 129 129 ASP D 0 0 -107.1 134.9 -179.9 999.9 999.9 151.1 0 0.0 0 0.0 0 0.0 0 0.0 5 7 130 130 SER S 0 0 -74.7 999.9 999.9 999.9 999.9 128.8 0 0.0 0 0.0 0 0.0 0 0.0 2 6 1i16-.pdb 1I16 CYTOKINE MOL_ID: 1; MOL_ID: 1; author author Kabs/Sand SS SSSS SS S SSSEEEEEEEEE SSSTTEEEE SSS EEEEE SS SSS TT EEE SS BGGGS HHHHHH Kabs/Sand chirality ---+++--++-++-+++--+---+--+---+----++-+--+---+-----++------+++--++++++---+--++---+--++--+++--++++++ chirality bends SS SSSS SS S SSS SSSSS SSS SS SSS SS SS SSSS SSSSSS bends turns TTTT TTTT TTTTTTTTTTTT turns 5-turns 5-turns 3-turns >33< >33< >>3<< >33 3-turns bridge-2 A bridge-2 bridge-1 AAAAAAAAA BBBB BB*BB C*C A bridge-1 sheets AAAAAAAAA BBBB BBBBB AAA sheets 4-turns >>>>XXX 4-turns summary SS SSSS SS S SSeEEEEEEEEEe SStTeEEEE SSS EEEEEeSS SSS tTTteEEE SS BGGGghHHHHHH summary sequence MPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPLTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWN sequence 10 20 30 40 50 60 70 80 90 100 author author Kabs/Sand HHHTS SSEEEEEEEEESS S SS Kabs/Sand chirality ++++--+-----+--++-+++++-++-+ chirality bends SSSSS SS SS S SS bends turns TTTTT turns 5-turns 5-turns 3-turns <>33< 3-turns bridge-2 CC bridge-2 bridge-1 AAAAAAAAA bridge-1 sheets AAAAAAAAA sheets 4-turns <<<< 4-turns summary HHHht SeEEEEEEEEEeS S SS summary sequence IIKALPDGPVTIVIRRKSLQSKETTAAGDS sequence 110 120 130