HEADER HYDROLASE/DNA COMPND MOL_ID: 1; COMPND 2 MOLECULE: DNA ENDONUCLEASE I-CREI; COMPND 3 CHAIN: A, B; COMPND 4 SYNONYM: I-CREI HOMING ENDONUCLEASE; COMPND 5 EC: 3.1.-.-; COMPND 6 ENGINEERED: YES; COMPND 7 MOL_ID: 2; COMPND 8 MOLECULE: 5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*A)- COMPND 9 3'; COMPND 10 CHAIN: C; COMPND 11 ENGINEERED: YES; COMPND 12 MOL_ID: 3; COMPND 13 MOLECULE: 5'-D(P*GP*AP*CP*AP*GP*TP*TP*TP*CP*G)-3'; COMPND 14 CHAIN: D; COMPND 15 ENGINEERED: YES; COMPND 16 MOL_ID: 4; COMPND 17 MOLECULE: 5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*C)- COMPND 18 3'; COMPND 19 CHAIN: E; COMPND 20 ENGINEERED: YES; COMPND 21 MOL_ID: 5; COMPND 22 MOLECULE: 5'-D(P*GP*AP*CP*GP*TP*TP*TP*TP*GP*C)-3'; COMPND 23 CHAIN: F; COMPND 24 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: CHLAMYDOMONAS REINHARDTII; SOURCE 3 ORGANISM_COMMON: GREEN ALGAE; SOURCE 4 GENE: ENCODED BY AN ORF WITHIN CR.LSU INTRON OF SOURCE 5 CHLOROPLAST 23S RRNA GENE; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 7 EXPRESSION_SYSTEM_COMMON: BACTERIA; SOURCE 8 EXPRESSION_SYSTEM_STRAIN: 'BL21(DE3); SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 10 EXPRESSION_SYSTEM_PLASMID: PI-CREI; SOURCE 11 MOL_ID: 2; SOURCE 12 SYNTHETIC: YES; SOURCE 13 MOL_ID: 3; SOURCE 14 SYNTHETIC: YES; SOURCE 15 MOL_ID: 4; SOURCE 16 SYNTHETIC: YES; SOURCE 17 MOL_ID: 5; SOURCE 18 SYNTHETIC: YES RESOLUTION SOURCE : ALS AUTHOR B.CHEVALIER,R.J.MONNAT,B.L.STODDARD EXPDTA X-RAY DIFFRACTION JRNL AUTH B.CHEVALIER,R.J.MONNAT,B.L.STODDARD JRNL TITL THE LAGLIDADG HOMING ENDONUCLEASE I-CREI SHARES JRNL TITL 2 THREE METALS BETWEEN TWO ACTIVE SITES JRNL REF NAT.STRUCT.BIOL. V. 8 312 2001 JRNL REFN ASTM NSBIEW US ISSN 1072-8368 RESOLUTION 1.80 ANGSTROMS.

Sequence in PIR format:

>P1;1g9zA structureX:1g9zA: 2 :A: 153 :A:5'-d(p*gp*ap*cp*gp*tp*tp*tp*tp*gp*c)-3':chlamydomonas reinhardtii: 9.99: 9.99 NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSLTFQVTQKTQRRWFLDKLVDEIGVGYVRDRGSVSDY ILSEIKPLHNFLTQLQPFLKLKQKQANLVLKIIEQLPSAKESPDKFLEVCTWVDQIAALNDSKTRKTTSETVRAV LD*

View SSTRUC file for 1g9zA

Rasmol View of 1g9zA