Secondary structure calculation program - copyright by David Keith Smith, 1989
1f1zA.pdb
1F1Z DNA BINDING PROTEIN MOL_ID: 1; MOL_ID: 1;
Sequence length - 260
A A K K hydrogen bonding Ooi's
strk chain/ l amino u & S structure bridge dihedral angles donor acceptor donor acceptor N N
num seq.no t acids t S + patterns partners phi psi omega alpha kappa tco to/energy fr/energy to/energy fr/energy 8 14
1 A 8 PHE F 0 0 999.9 -62.8 -178.7 999.9 999.9 999.9 0 0.0 0 0.0 0 0.0 0 0.0 3 27
2 A 9 SER S - 0 0 -120.4 135.6 179.9 -149.6 999.9 158.2 0 0.0 0 0.0 0 0.0 0 0.0 6 30
3 A 10 GLU E t > T - 0 0 -85.9 -174.3 179.8 -70.0 53.7 100.1 0 0.0 6 -2.3 0 0.0 0 0.0 6 20
4 A 11 VAL V T T > TS+ 0 0 -52.3 -17.5 178.1 87.8 119.7 46.6 0 0.0 7 -1.6 0 0.0 0 0.0 6 20
5 A 12 GLN Q T h > > T + 0 0 -57.7 -12.4 178.0 101.4 54.4 53.2 0 0.0 8 -3.1 0 0.0 9 -0.9 7 21
6 A 13 ILE I H H > < T + 0 0 -42.1 -33.3 179.4 74.8 65.6 37.6 3 -2.3 10 -2.3 0 0.0 0 0.0 8 34
7 A 14 ALA A H H > < TS+ 0 0 -51.8 -38.0 179.3 47.8 95.0 32.4 4 -1.6 11 -1.0 0 0.0 0 0.0 7 25
8 A 15 ARG R H H > < TS+ 0 0 -72.7 -40.3 178.7 55.0 106.3 26.5 5 -3.1 12 -1.8 0 0.0 0 0.0 7 19
9 A 16 ARG R H H < >TS+ 0 0 -58.1 -36.8 178.4 55.3 105.5 27.2 5 -0.9 14 -1.3 0 0.0 0 0.0 9 33
10 A 17 ILE I H H < >5TS+ 0 0 -62.4 -36.6 179.4 48.9 107.8 30.5 6 -2.3 13 -0.8 0 0.0 0 0.0 9 32
11 A 18 LYS K H H < 35TS+ 0 0 -74.5 -29.3 178.2 69.9 96.0 39.3 7 -1.0 0 0.0 0 0.0 0 0.0 7 19
12 A 19 GLU E T h < 35TS- 0 0 -62.1 -14.8 179.8 -109.0 119.1 51.7 8 -1.8 0 0.0 0 0.0 0 0.0 6 20
13 A 20 GLY G T T X5T + 0 0 99.2 0.5 -179.9 170.0 57.1 66.0 10 -0.8 16 -3.1 0 0.0 0 0.0 9 28
14 A 21 ARG R T T 3 T + 0 0 -146.4 -175.5 -179.4 23.5 63.6 149.9 0 0.0 21 -2.5 0 0.0 0 0.0 10 35
19 A 26 GLY G G G > TS- 0 0 52.7 -136.8 179.9 -13.3 133.0 104.3 0 0.0 22 -1.3 0 0.0 0 0.0 12 37
20 A 27 LYS K G G 3 TS+ 0 0 -67.7 -15.2 -178.4 60.0 140.6 49.8 0 0.0 0 0.0 0 0.0 0 0.0 7 33
21 A 28 ASP D G G < TS+ 0 0 -90.1 -11.8 179.8 106.0 78.7 52.1 18 -2.5 0 0.0 0 0.0 0 0.0 7 31
22 A 29 TYR Y g < T - 0 0 -71.9 135.0 178.7 -158.0 54.4 118.4 19 -1.3 0 0.0 0 0.0 0 0.0 11 41
23 A 30 ILE I - 0 0 -115.4 121.8 179.9 -126.1 18.4 164.8 16 -1.2 15 -3.1 0 0.0 0 0.0 10 46
24 A 31 PRO P - 0 0 -61.5 157.7 178.6 -133.8 9.3 101.6 0 0.0 0 0.0 0 0.0 0 0.0 13 54
25 A 32 TRP W S S S+ 0 0 -78.9 -40.2 177.9 37.2 96.9 31.3 75 -1.9 0 0.0 0 0.0 0 0.0 10 48
26 A 33 LEU L S S S- 0 0 -110.3 144.7 179.3 -166.2 78.0 152.2 0 0.0 0 0.0 0 0.0 0 0.0 8 46
27 A 34 THR T g > T - 0 0 -121.3 177.1 180.0 -89.4 42.1 133.7 0 0.0 30 -1.9 0 0.0 0 0.0 11 45
28 A 35 VAL V G G > TS+ 0 0 -60.2 -23.3 179.0 67.2 121.9 41.8 0 0.0 31 -1.0 0 0.0 0 0.0 11 33
29 A 36 GLN Q G G 3 TS+ 0 0 -69.9 -20.5 179.6 62.6 92.5 44.0 0 0.0 0 0.0 0 0.0 0 0.0 5 26
30 A 37 GLU E G G < TS+ 0 0 -93.6 30.3 -179.2 136.4 75.1 94.0 27 -1.9 0 0.0 0 0.0 0 0.0 7 34
31 A 38 VAL V g < T - 0 0 -86.7 124.0 180.0 -151.9 40.3 133.9 28 -1.0 0 0.0 0 0.0 0 0.0 10 29
32 A 39 PRO P - 0 0 -96.2 156.9 178.8 -13.8 46.6 130.5 0 0.0 0 0.0 0 0.0 0 0.0 6 22
33 A 40 SER S S S S- 0 0 54.1 -159.3 178.1 -40.1 114.1 93.0 0 0.0 0 0.0 0 0.0 0 0.0 4 15
34 A 41 SER S S S S+ 0 0 -70.5 -13.7 179.7 131.7 99.9 51.5 0 0.0 0 0.0 0 0.0 0 0.0 4 15
35 A 42 GLY G - 0 0 -44.6 109.2 -179.6 -136.4 56.9 100.5 0 0.0 0 0.0 0 0.0 0 0.0 9 26
36 A 43 ARG R - 0 0 -75.3 142.4 179.4 -148.4 15.1 120.6 0 0.0 0 0.0 0 0.0 0 0.0 8 31
37 A 44 SER S - 0 0 -106.9 162.8 -178.6 -152.5 8.2 135.2 0 0.0 0 0.0 0 0.0 0 0.0 11 41
38 A 45 HIS H E E AA - 51 0 -142.3 143.7 177.3 -161.0 15.8 174.9 51 -2.8 51 -2.8 0 0.0 40 -0.6 12 53
39 A 46 ARG R E E AA + 50 0 -122.8 103.0 -177.3 171.5 29.3 163.8 0 0.0 0 0.0 0 0.0 0 0.0 10 55
40 A 47 ILE I E E AA - 49 0 -117.9 143.7 179.3 -122.8 29.4 155.9 49 -1.4 49 -3.3 38 -0.6 0 0.0 10 59
41 A 48 TYR Y E E AA - 48 0 -82.8 153.3 179.7 -148.3 21.4 122.8 231 -2.7 0 0.0 0 0.0 0 0.0 11 59
42 A 49 SER S e > T - 0 0 -126.4 132.0 178.1 -158.5 13.8 169.1 47 -1.8 46 -1.5 0 0.0 0 0.0 12 57
43 A 50 HIS H T T 4 TS+ 0 0 -71.7 -39.6 -179.2 52.9 96.6 26.9 0 0.0 0 0.0 0 0.0 0 0.0 10 46
44 A 51 LYS K T T 4 TS+ 0 0 -61.9 -49.8 -177.8 25.8 123.0 19.6 0 0.0 0 0.0 0 0.0 0 0.0 8 43
45 A 52 THR T T T 4 TS- 0 0 -90.9 -18.2 -179.3 -135.9 93.8 51.1 0 0.0 0 0.0 0 0.0 0 0.0 11 46
46 A 53 GLY G S t < TS+ 0 0 71.1 16.5 179.6 95.5 71.1 47.1 42 -1.5 0 0.0 0 0.0 0 0.0 9 40
47 A 54 ARG R S e S- 0 0 -133.1 173.4 178.9 -99.1 80.2 143.4 0 0.0 42 -1.8 0 0.0 0 0.0 10 42
48 A 55 VAL V E E AA - 41 0 -96.0 129.2 -177.5 -148.3 38.4 145.9 0 0.0 0 0.0 0 0.0 0 0.0 9 54
49 A 56 HIS H E E AA - 40 0 -100.2 151.1 179.9 -130.8 7.4 135.1 40 -3.3 40 -1.4 0 0.0 51 -0.6 11 60
50 A 57 HIS H E E AA + 39 0 -105.5 114.3 -179.4 179.2 25.2 151.9 0 0.0 0 0.0 0 0.0 0 0.0 14 58
51 A 58 LEU L E E AA - 38 0 -113.6 138.0 -176.9 -156.6 17.2 155.4 38 -2.8 38 -2.8 49 -0.6 0 0.0 14 56
52 A 59 LEU L + 0 0 -99.6 6.5 178.3 20.4 69.9 69.7 0 0.0 0 0.0 0 0.0 0 0.0 15 42
53 A 60 SER S S h > TS- 0 0 -163.2 163.8 179.4 -106.5 77.5 168.0 0 0.0 57 -1.8 0 0.0 0 0.0 10 47
54 A 61 ASP D H H > TS+ 0 0 -64.2 -32.4 -179.8 57.3 120.3 31.8 0 0.0 58 -2.5 0 0.0 0 0.0 11 50
55 A 62 LEU L H H > TS+ 0 0 -65.3 -44.4 178.9 46.8 106.5 23.6 0 0.0 59 -1.7 0 0.0 0 0.0 11 51
56 A 63 GLU E H H > TS+ 0 0 -65.6 -34.8 179.4 54.8 110.2 31.7 0 0.0 60 -2.9 0 0.0 0 0.0 11 58
57 A 64 LEU L H H X TS+ 0 0 -62.3 -48.6 179.6 52.0 105.7 18.4 53 -1.8 61 -2.9 0 0.0 0 0.0 11 62
58 A 65 ALA A H H X TS+ 0 0 -56.5 -37.1 -179.7 45.9 112.3 29.7 54 -2.5 62 -1.4 0 0.0 0 0.0 12 62
59 A 66 VAL V H H X TS+ 0 0 -73.3 -42.8 179.6 51.8 111.1 22.7 55 -1.7 63 -2.2 0 0.0 0 0.0 10 58
60 A 67 PHE F H H X TS+ 0 0 -57.8 -48.1 179.8 52.1 107.6 22.8 56 -2.9 64 -3.2 0 0.0 0 0.0 9 71
61 A 68 LEU L H H X TS+ 0 0 -56.8 -40.9 178.9 50.2 109.5 27.1 57 -2.9 65 -1.8 0 0.0 0 0.0 9 72
62 A 69 SER S H H X TS+ 0 0 -65.4 -38.7 179.8 47.6 112.5 27.9 58 -1.4 66 -1.4 0 0.0 0 0.0 9 59
63 A 70 LEU L H H < TS+ 0 0 -67.3 -48.8 179.9 50.3 109.9 19.1 59 -2.2 0 0.0 0 0.0 0 0.0 9 56
64 A 71 GLU E H H < TS+ 0 0 -58.5 -32.3 179.5 54.5 109.4 33.3 60 -3.2 0 0.0 0 0.0 0 0.0 12 55
65 A 72 TRP W H H < TS+ 0 0 -69.8 -37.8 -178.4 98.8 85.8 32.9 61 -1.8 0 0.0 0 0.0 0 0.0 10 47
66 A 73 GLU E h < > T - 0 0 -60.5 126.3 179.9 -154.7 61.7 111.4 62 -1.4 69 -0.8 0 0.0 0 0.0 7 36
67 A 74 SER S T T 3 TS+ 0 0 -66.3 -39.0 -178.9 56.6 94.3 30.3 0 0.0 0 0.0 0 0.0 0 0.0 6 31
68 A 75 SER S T e 3 TS+ 0 0 -70.3 -14.7 -179.3 96.8 84.8 52.6 0 0.0 113 -2.1 0 0.0 0 0.0 8 27
69 A 76 VAL V E E BB < T + 112 0 -81.5 131.0 179.7 171.7 42.4 130.7 66 -0.8 0 0.0 0 0.0 0 0.0 12 37
70 A 77 LEU L E E B* + 0 0 -105.8 -37.6 179.6 14.7 64.0 44.2 111 -3.1 0 0.0 0 0.0 0 0.0 11 39
71 A 78 ASP D E E BB - 111 0 -143.2 149.0 179.9 -161.7 53.2 171.4 111 -1.2 111 -1.6 0 0.0 0 0.0 11 51
72 A 79 ILE I E E BB - 110 0 -132.8 109.0 179.4 -176.5 7.6 164.6 0 0.0 74 -0.7 0 0.0 0 0.0 13 61
73 A 80 ARG R E E BB - 109 0 -111.2 95.1 -177.6 -162.0 17.6 151.0 109 -2.9 109 -1.5 0 0.0 0 0.0 12 61
74 A 81 GLU E E E BB + 108 0 -77.2 159.3 -178.9 20.2 65.1 109.7 72 -0.7 0 0.0 0 0.0 0 0.0 12 68
75 A 82 GLN Q E E B* S- 0 0 46.3 53.4 -179.1 -170.4 83.1 22.5 107 -2.4 25 -1.9 0 0.0 0 0.0 15 57
76 A 83 PHE F E E BB - 106 0 -79.5 123.2 -179.7 -127.0 16.3 129.5 106 -2.1 106 -3.1 0 0.0 0 0.0 13 55
77 A 84 PRO P E E BB - 105 0 -73.3 138.7 179.4 -138.6 12.2 115.7 0 0.0 0 0.0 0 0.0 0 0.0 10 52
78 A 85 LEU L e - 0 0 -99.1 130.2 -178.9 -97.8 35.4 146.6 104 -3.1 0 0.0 0 0.0 0 0.0 11 53
79 A 86 LEU L h > > T - 0 0 -51.6 118.1 -179.7 -142.4 28.4 104.3 0 0.0 83 -2.2 0 0.0 82 -0.6 8 39
80 A 87 PRO P H H > 3 TS+ 0 0 -50.8 -42.5 -179.7 56.4 98.7 33.9 0 0.0 84 -2.5 0 0.0 0 0.0 8 40
81 A 88 SER S H H > 3 TS+ 0 0 -60.9 -39.5 179.1 46.0 110.5 23.6 0 0.0 85 -1.9 0 0.0 0 0.0 6 30
82 A 89 ASP D H H > < TS+ 0 0 -67.7 -45.3 -179.9 47.6 113.7 24.0 79 -0.6 86 -2.3 0 0.0 0 0.0 9 34
83 A 90 THR T H H X TS+ 0 0 -67.7 -33.5 178.4 51.3 110.4 33.9 79 -2.2 87 -2.4 0 0.0 0 0.0 14 40
84 A 91 ARG R H H X TS+ 0 0 -67.5 -44.0 179.0 49.1 111.3 21.5 80 -2.5 88 -2.7 0 0.0 0 0.0 9 36
85 A 92 GLN Q H H X TS+ 0 0 -59.4 -46.3 179.1 46.9 112.9 24.5 81 -1.9 89 -3.0 0 0.0 0 0.0 8 32
86 A 93 ILE I H H X TS+ 0 0 -63.3 -43.2 180.0 51.9 110.6 24.8 82 -2.3 90 -1.8 0 0.0 0 0.0 11 34
87 A 94 ALA A H H X >TS+ 0 0 -59.6 -43.3 179.6 46.1 113.4 19.9 83 -2.4 92 -1.9 0 0.0 91 -1.9 12 36
88 A 95 ILE I H H < 5TS+ 0 0 -64.2 -51.7 -180.0 48.9 112.1 17.9 84 -2.7 0 0.0 0 0.0 0 0.0 10 24
89 A 96 ASP D H H < 5TS+ 0 0 -62.6 -23.2 -179.9 39.3 119.5 42.8 85 -3.0 0 0.0 0 0.0 0 0.0 6 23
90 A 97 SER S H H < 5TS- 0 0 -101.4 -18.4 -178.9 -122.3 104.3 49.5 86 -1.8 0 0.0 0 0.0 0 0.0 6 26
91 A 98 GLY G T h < 5T + 0 0 79.9 29.3 179.7 138.9 64.8 36.5 87 -1.9 0 0.0 0 0.0 0 0.0 6 22
92 A 99 ILE I t TS- 100 0 -140.4 112.6 -179.4 -17.7 82.3 164.6 100 -2.6 100 -1.7 0 0.0 0 0.0 8 19
98 A 105 ARG R T T 3 TS- 0 0 57.5 46.7 179.2 -55.5 126.3 22.8 0 0.0 0 0.0 0 0.0 0 0.0 4 16
99 A 106 GLY G T T 3 TS+ 0 0 76.7 -22.1 179.1 115.1 114.3 80.0 0 0.0 101 -0.5 0 0.0 0 0.0 5 17
100 A 107 VAL V E E CC < T - 97 0 -84.6 121.9 178.6 -128.3 69.2 136.6 97 -1.7 97 -2.6 0 0.0 0 0.0 8 22
101 A 108 ASP D E E CC - 96 0 -69.1 133.7 -177.7 -129.6 29.2 123.7 99 -0.5 0 0.0 0 0.0 0 0.0 8 34
102 A 109 GLN Q e - 0 0 -88.3 155.7 178.1 -113.1 10.9 120.5 95 -2.7 104 -0.6 0 0.0 0 0.0 9 43
103 A 110 VAL V - 0 0 -88.8 119.0 -179.8 -142.9 34.5 142.2 0 0.0 0 0.0 0 0.0 0 0.0 9 49
104 A 111 MET M e - 0 0 -79.0 158.9 -179.7 -165.1 16.8 115.4 102 -0.6 78 -3.1 0 0.0 0 0.0 11 60
105 A 112 SER S E E BB - 77 0 -150.0 143.5 176.7 -133.2 19.2 179.0 0 0.0 0 0.0 0 0.0 0 0.0 9 62
106 A 113 THR T E E BB - 76 0 -92.1 140.8 -177.7 -129.2 25.5 137.8 76 -3.1 76 -2.1 0 0.0 0 0.0 14 61
107 A 114 ASP D E E B* S+ 0 0 -59.2 -41.5 179.1 11.9 87.4 25.6 0 0.0 75 -2.4 0 0.0 0 0.0 12 62
108 A 115 PHE F E E BBD - 74 122 -144.4 133.9 175.5 -173.3 51.9 175.0 122 -2.2 122 -2.8 0 0.0 110 -0.5 12 63
109 A 116 LEU L E E BBD - 73 121 -120.7 116.7 -178.4 -172.6 26.9 176.3 73 -1.5 73 -2.9 0 0.0 0 0.0 14 63
110 A 117 VAL V E E BBD - 72 120 -123.3 140.8 179.3 -151.9 16.9 161.9 120 -2.9 120 -2.2 108 -0.5 0 0.0 12 58
111 A 118 ASP D E E BBD - 71 119 -104.1 146.5 -178.7 -159.1 15.9 146.8 71 -1.6 70 -3.1 0 0.0 71 -1.2 11 41
112 A 119 CYS C E E BB - 69 0 -125.5 162.3 179.6 -149.8 20.7 146.2 118 -2.3 0 0.0 0 0.0 0 0.0 12 33
113 A 120 LYS K S e S- 0 0 -101.9 -26.9 179.8 -7.7 91.9 46.0 68 -2.1 0 0.0 0 0.0 0 0.0 8 22
114 A 121 ASP D S S S+ 0 0 -157.6 18.8 178.3 120.9 92.4 74.5 0 0.0 0 0.0 0 0.0 0 0.0 7 16
115 A 122 GLY G S S S- 0 0 -75.5 -177.2 179.7 -84.1 84.1 96.2 0 0.0 0 0.0 0 0.0 0 0.0 7 20
116 A 123 PRO P S S S+ 0 0 -57.9 -34.7 179.3 29.9 119.2 31.4 0 0.0 0 0.0 0 0.0 0 0.0 4 20
117 A 124 PHE F S S S- 0 0 -129.7 138.4 -178.8 -139.1 73.6 168.9 0 0.0 0 0.0 0 0.0 0 0.0 8 25
118 A 125 GLU E S e S+ 0 0 -71.1 -9.1 -177.6 20.3 89.8 54.4 0 0.0 112 -2.3 0 0.0 0 0.0 9 26
119 A 126 GLN Q E E BD + 111 0 -160.5 141.8 177.3 166.3 65.0 169.0 0 0.0 0 0.0 0 0.0 0 0.0 10 34
120 A 127 PHE F E E BD - 110 0 -156.3 158.7 -178.1 -133.8 21.9 168.5 110 -2.2 110 -2.9 0 0.0 0 0.0 11 48
121 A 128 ALA A E E BDe - 109 153 -117.9 155.3 -179.2 -159.4 13.2 149.7 152 -2.7 154 -1.4 0 0.0 0 0.0 14 52
122 A 129 ILE I E E BDe - 108 154 -138.9 135.8 176.6 -162.9 7.9 174.1 108 -2.8 108 -2.2 0 0.0 124 -0.5 12 57
123 A 130 GLN Q E E B e - 0 155 -117.1 122.6 179.5 -152.0 16.4 170.1 154 -2.5 156 -2.6 0 0.0 0 0.0 11 59
124 A 131 VAL V E E B e + 0 156 -98.2 128.5 179.1 150.6 28.4 149.1 122 -0.5 0 0.0 0 0.0 0 0.0 11 54
125 A 132 LYS K e - 0 0 -155.7 140.1 179.4 -106.3 47.6 169.4 156 -2.2 0 0.0 0 0.0 0 0.0 11 47
126 A 133 PRO P g > > T - 0 0 -68.5 140.0 -178.5 -117.2 36.5 121.1 0 0.0 129 -1.9 0 0.0 130 -0.5 10 32
127 A 134 ALA A G G 4 > TS+ 0 0 -44.0 -42.4 179.7 60.9 113.1 32.3 0 0.0 130 -1.6 0 0.0 0 0.0 9 25
128 A 135 ALA A G G 4 > TS+ 0 0 -57.3 -33.1 -178.5 68.3 91.3 33.6 0 0.0 131 -2.1 0 0.0 0 0.0 6 20
129 A 136 ALA A G G 4 X TS+ 0 0 -62.0 -22.0 179.7 68.2 86.0 43.6 126 -1.9 132 -1.3 0 0.0 0 0.0 8 25
130 A 137 LEU L G G < < TS+ 0 0 -75.3 -6.7 179.2 71.9 84.7 55.5 127 -1.6 0 0.0 126 -0.5 0 0.0 10 25
131 A 138 GLN Q G G < TS+ 0 0 -86.7 3.1 -179.0 88.9 81.8 68.2 128 -2.1 133 -0.5 0 0.0 0 0.0 8 16
132 A 139 ASP D S h > < TS- 0 0 -107.0 125.2 -178.6 -146.5 71.4 155.7 129 -1.3 136 -2.0 0 0.0 0 0.0 8 15
133 A 140 GLU E H H > TS+ 0 0 -56.4 -44.1 -178.9 51.5 97.1 31.6 131 -0.5 137 -2.1 0 0.0 0 0.0 7 20
134 A 141 ARG R H H > TS+ 0 0 -64.3 -38.3 179.0 52.3 107.7 27.5 0 0.0 138 -2.5 0 0.0 0 0.0 6 25
135 A 142 THR T H H > TS+ 0 0 -61.4 -48.3 179.6 51.3 108.7 16.1 0 0.0 139 -2.9 0 0.0 0 0.0 10 31
136 A 143 LEU L H H X TS+ 0 0 -54.9 -43.5 179.5 51.3 108.9 26.2 132 -2.0 140 -2.1 0 0.0 0 0.0 10 33
137 A 144 GLU E H H X TS+ 0 0 -60.5 -48.5 179.6 46.3 111.5 18.2 133 -2.1 141 -2.0 0 0.0 0 0.0 9 33
138 A 145 LYS K H H X TS+ 0 0 -61.2 -38.2 179.5 53.5 111.1 25.8 134 -2.5 142 -2.2 0 0.0 0 0.0 10 48
139 A 146 LEU L H H X TS+ 0 0 -65.8 -33.8 178.5 52.5 106.3 32.8 135 -2.9 143 -2.9 0 0.0 0 0.0 8 46
140 A 147 GLU E H H X TS+ 0 0 -68.4 -40.9 179.3 52.3 107.0 24.6 136 -2.1 144 -2.8 0 0.0 0 0.0 8 46
141 A 148 LEU L H H X TS+ 0 0 -58.2 -47.4 -179.0 43.8 114.0 17.1 137 -2.0 145 -1.8 0 0.0 0 0.0 12 48
142 A 149 GLU E H H X TS+ 0 0 -64.8 -44.8 -179.7 52.5 111.9 24.4 138 -2.2 146 -1.9 0 0.0 0 0.0 11 58
143 A 150 ARG R H H X TS+ 0 0 -58.4 -48.0 -179.9 47.2 110.7 21.8 139 -2.9 147 -2.4 0 0.0 0 0.0 9 48
144 A 151 ARG R H H X TS+ 0 0 -61.6 -39.0 -178.9 60.1 106.0 31.5 140 -2.8 148 -2.7 0 0.0 0 0.0 9 42
145 A 152 TYR Y H H X TS+ 0 0 -56.0 -51.4 -179.7 37.0 112.1 17.9 141 -1.8 149 -0.9 0 0.0 0 0.0 12 46
146 A 153 TRP W H H < >>TS+ 0 0 -70.8 -36.5 178.6 57.4 113.3 28.7 142 -1.9 151 -1.7 0 0.0 149 -0.5 12 42
147 A 154 GLN Q H H < >5TS+ 0 0 -58.6 -40.2 179.5 57.7 101.4 25.2 143 -2.4 150 -1.5 0 0.0 0 0.0 10 31
148 A 155 GLN Q H H < 35TS+ 0 0 -58.1 -34.3 -178.9 43.2 111.1 33.9 144 -2.7 0 0.0 0 0.0 0 0.0 7 27
149 A 156 LYS K T h < <5TS- 0 0 -95.6 5.1 179.6 -121.7 109.7 66.0 145 -0.9 0 0.0 146 -0.5 0 0.0 6 27
150 A 157 GLN Q T T <5T + 0 0 54.9 43.7 178.3 137.9 65.6 26.3 147 -1.5 0 0.0 0 0.0 0 0.0 6 21
151 A 158 ILE I t T - 0 0 -120.7 177.4 -179.9 -86.4 48.8 133.3 155 -0.5 160 -1.9 0 0.0 0 0.0 11 36
158 A 165 ASP D G G > TS+ 0 0 -56.9 -32.6 179.8 63.9 120.5 37.7 0 0.0 161 -1.7 0 0.0 0 0.0 9 34
159 A 166 LYS K G G 3 TS+ 0 0 -77.6 9.1 177.6 62.0 94.7 68.3 0 0.0 0 0.0 0 0.0 0 0.0 6 26
160 A 167 GLU E G G < TS+ 0 0 -106.4 -5.2 -179.7 109.6 80.2 67.6 157 -1.9 162 -0.5 0 0.0 0 0.0 6 31
161 A 168 ILE I S g < TS- 0 0 -79.1 122.1 178.6 -125.6 74.0 130.9 158 -1.7 0 0.0 0 0.0 0 0.0 9 34
162 A 169 ASN N h > T - 0 0 -64.2 133.2 -179.6 -128.8 18.2 113.6 160 -0.5 166 -2.6 0 0.0 0 0.0 8 31
163 A 170 PRO P H H > TS+ 0 0 -53.2 -41.7 179.6 49.8 108.2 27.9 0 0.0 167 -2.6 0 0.0 0 0.0 6 25
164 A 171 VAL V H H > TS+ 0 0 -64.2 -44.3 178.9 49.5 110.8 22.0 0 0.0 168 -2.6 0 0.0 0 0.0 10 29
165 A 172 VAL V H H > TS+ 0 0 -61.0 -37.6 -179.7 54.1 110.0 24.5 0 0.0 169 -2.0 0 0.0 0 0.0 13 42
166 A 173 LYS K H H X TS+ 0 0 -62.6 -50.5 -178.8 42.3 110.8 17.8 162 -2.6 170 -2.0 0 0.0 0 0.0 10 36
167 A 174 GLU E H H X TS+ 0 0 -66.7 -34.2 179.1 57.4 110.3 32.1 163 -2.6 171 -1.5 0 0.0 0 0.0 9 34
168 A 175 ASN N H H X TS+ 0 0 -62.2 -39.5 179.7 47.6 108.5 25.4 164 -2.6 172 -2.5 0 0.0 0 0.0 11 44
169 A 176 ILE I H H X TS+ 0 0 -67.9 -40.3 179.0 54.9 108.0 26.5 165 -2.0 173 -2.2 0 0.0 0 0.0 9 49
170 A 177 GLU E H H < TS+ 0 0 -62.9 -30.3 178.7 43.5 112.7 39.5 166 -2.0 0 0.0 0 0.0 0 0.0 8 35
171 A 178 TRP W H H < > TS+ 0 0 -80.8 -41.9 -177.5 44.6 117.6 26.2 167 -1.5 174 -0.6 0 0.0 0 0.0 7 36
172 A 179 LEU L H H < 3 TS+ 0 0 -77.3 -18.3 -176.6 44.7 116.0 46.2 168 -2.5 0 0.0 0 0.0 0 0.0 11 45
173 A 180 TYR Y T h < 3 T + 0 0 -121.9 30.1 176.6 109.7 63.3 87.8 169 -2.2 0 0.0 0 0.0 0 0.0 8 40
174 A 181 SER S S t < TS- 0 0 -89.6 40.4 -177.0 -102.7 99.3 97.4 171 -0.6 0 0.0 0 0.0 0 0.0 6 24
175 A 182 VAL V + 0 0 31.6 90.9 179.5 154.2 67.2 24.6 0 0.0 0 0.0 0 0.0 0 0.0 6 28
176 A 183 LYS K - 0 0 -101.4 -59.0 178.9 -4.4 65.7 34.1 0 0.0 0 0.0 0 0.0 0 0.0 6 27
177 A 184 THR T + 0 0 -142.1 142.0 178.7 162.8 45.4 174.7 0 0.0 0 0.0 0 0.0 0 0.0 5 32
178 A 185 GLU E + 0 0 -133.6 -1.3 -179.5 120.6 45.4 76.8 0 0.0 180 -1.0 0 0.0 0 0.0 7 32
179 A 186 GLU E - 0 0 -73.9 100.7 180.0 -168.4 44.3 126.1 0 0.0 181 -1.5 0 0.0 0 0.0 5 29
180 A 187 VAL V + 0 0 -89.5 63.4 -179.9 177.4 19.6 121.1 178 -1.0 0 0.0 0 0.0 0 0.0 7 36
181 A 188 SER S h > > T - 0 0 -66.9 150.7 177.9 -120.7 43.7 112.3 179 -1.5 185 -2.3 0 0.0 184 -1.0 6 22
182 A 189 ALA A H H > 3 TS+ 0 0 -60.8 -23.8 179.2 57.1 119.4 38.3 0 0.0 186 -1.2 0 0.0 0 0.0 8 25
183 A 190 GLU E H H > 3 TS+ 0 0 -76.3 -30.9 178.9 50.2 103.2 42.3 0 0.0 187 -0.6 0 0.0 0 0.0 7 27
184 A 191 LEU L H H 4 X TS+ 0 0 -72.7 -47.4 178.9 46.0 113.4 21.6 181 -1.0 187 -1.1 0 0.0 0 0.0 11 37
185 A 192 LEU L H H < > TS+ 0 0 -63.8 -29.4 179.0 69.4 99.7 37.7 181 -2.3 188 -2.0 0 0.0 0 0.0 12 37
186 A 193 ALA A H H < > TS+ 0 0 -57.0 -32.5 -179.8 63.8 90.6 30.6 182 -1.2 189 -0.5 0 0.0 0 0.0 8 29
187 A 194 GLN Q T h X < TS+ 0 0 -68.2 -12.1 -178.6 80.5 83.3 51.5 184 -1.1 191 -2.3 183 -0.6 0 0.0 8 42
188 A 195 LEU L H H > < TS+ 0 0 -61.7 -54.0 -179.1 44.1 92.0 16.3 185 -2.0 192 -2.7 0 0.0 0 0.0 12 44
189 A 196 SER S H H > < TS+ 0 0 -58.8 -52.0 179.6 44.5 117.0 20.9 186 -0.5 193 -1.8 0 0.0 0 0.0 9 29
190 A 197 PRO P H H > TS+ 0 0 -56.5 -52.2 -179.5 48.3 116.0 13.4 0 0.0 194 -2.0 0 0.0 0 0.0 8 36
191 A 198 LEU L H H X TS+ 0 0 -55.9 -40.2 -179.9 57.5 106.4 30.7 187 -2.3 195 -2.5 0 0.0 0 0.0 8 51
192 A 199 ALA A H H X TS+ 0 0 -58.7 -45.2 -179.5 47.9 107.3 23.4 188 -2.7 196 -2.1 0 0.0 0 0.0 10 41
193 A 200 HIS H H H X TS+ 0 0 -61.4 -53.1 179.9 47.7 112.0 20.2 189 -1.8 197 -2.2 0 0.0 0 0.0 8 30
194 A 201 ILE I H H X TS+ 0 0 -52.7 -54.7 -179.4 46.1 114.6 15.4 190 -2.0 198 -2.8 0 0.0 0 0.0 8 41
195 A 202 LEU L H H < TS+ 0 0 -59.1 -35.7 179.1 56.8 109.2 34.2 191 -2.5 0 0.0 0 0.0 0 0.0 12 42
196 A 203 GLN Q H H < > TS+ 0 0 -64.1 -45.8 -178.9 37.1 113.8 20.3 192 -2.1 199 -0.9 0 0.0 0 0.0 9 30
197 A 204 GLU E H H < 3 TS+ 0 0 -75.9 -38.0 179.2 49.3 117.5 25.2 193 -2.2 0 0.0 0 0.0 0 0.0 6 26
198 A 205 LYS K T h < > TS+ 0 0 -85.5 24.6 -178.2 142.3 80.9 82.4 194 -2.8 201 -2.7 0 0.0 0 0.0 10 28
199 A 206 GLY G T T < T + 0 0 -32.0 -60.9 -178.1 43.9 62.3 42.7 196 -0.9 248 -2.4 0 0.0 0 0.0 9 28
200 A 207 ASP D T T 3 TS+ 0 0 -74.6 0.8 -179.3 104.2 94.8 62.4 0 0.0 0 0.0 0 0.0 0 0.0 7 26
201 A 208 GLU E S t < TS- 0 0 -87.4 150.2 178.5 -98.2 82.6 122.8 198 -2.7 247 -2.6 0 0.0 0 0.0 10 28
202 A 209 ASN N B h > A T - 246 0 -63.0 136.3 -178.3 -127.2 31.0 114.1 0 0.0 206 -2.1 0 0.0 0 0.0 11 32
203 A 210 ILE I H H > TS+ 0 0 -56.4 -34.1 -179.7 52.2 106.0 37.7 245 -2.6 207 -1.3 0 0.0 0 0.0 12 47
204 A 211 ILE I H H > TS+ 0 0 -72.6 -41.0 179.0 49.2 108.3 24.2 0 0.0 208 -1.9 0 0.0 0 0.0 8 38
205 A 212 ASN N H H > TS+ 0 0 -60.5 -52.3 -179.9 50.2 110.8 16.1 0 0.0 209 -2.7 0 0.0 0 0.0 8 33
206 A 213 VAL V H H X TS+ 0 0 -55.6 -33.7 179.7 55.8 108.2 30.7 202 -2.1 210 -2.2 0 0.0 0 0.0 12 44
207 A 214 CYS C H H X TS+ 0 0 -67.5 -40.7 179.7 44.5 108.7 26.4 203 -1.3 211 -1.9 0 0.0 0 0.0 11 48
208 A 215 LYS K H H X TS+ 0 0 -68.3 -44.8 179.3 55.2 110.9 20.7 204 -1.9 212 -2.5 0 0.0 0 0.0 12 32
209 A 216 GLN Q H H X TS+ 0 0 -53.7 -45.4 179.2 47.1 109.2 26.6 205 -2.7 213 -2.8 0 0.0 0 0.0 8 30
210 A 217 VAL V H H X TS+ 0 0 -65.4 -41.3 179.3 55.6 108.1 26.4 206 -2.2 214 -2.3 0 0.0 0 0.0 9 36
211 A 218 ASP D H H X >TS+ 0 0 -55.3 -47.8 179.5 41.9 113.7 18.9 207 -1.9 216 -2.5 0 0.0 215 -0.6 14 27
212 A 219 ILE I H H < >5TS+ 0 0 -61.6 -58.6 -178.5 55.8 110.2 12.4 208 -2.5 215 -1.8 0 0.0 0 0.0 9 19
213 A 220 ALA A H H < 35TS+ 0 0 -44.4 -45.4 -178.6 30.3 120.4 34.8 209 -2.8 0 0.0 0 0.0 0 0.0 6 20
214 A 221 TYR Y H H < 35TS- 0 0 -100.0 4.4 -179.8 -116.8 110.6 67.8 210 -2.3 0 0.0 0 0.0 0 0.0 6 21
215 A 222 ASP D T h < <5T + 0 0 55.9 66.5 178.1 153.2 58.1 7.1 212 -1.8 0 0.0 211 -0.6 0 0.0 6 16
216 A 223 LEU L t T - 0 0 -69.7 140.6 -178.7 -82.5 62.1 118.8 0 0.0 220 -2.5 0 0.0 0 0.0 6 17
218 A 225 LEU L T T 3 TS+ 0 0 -47.6 116.5 -179.9 23.3 118.9 100.6 0 0.0 0 0.0 0 0.0 0 0.0 7 21
219 A 226 GLY G T h > 3 TS+ 0 0 110.0 -10.6 -178.7 117.5 83.7 77.1 0 0.0 223 -2.4 0 0.0 0 0.0 10 27
220 A 227 LYS K H H > < TS+ 0 0 -54.5 -53.0 -179.3 42.4 79.7 24.1 217 -2.5 224 -1.9 0 0.0 0 0.0 9 31
221 A 228 THR T H H > TS+ 0 0 -64.4 -39.7 179.4 54.0 112.2 26.2 0 0.0 225 -3.0 0 0.0 0 0.0 11 46
222 A 229 LEU L H H > TS+ 0 0 -61.5 -38.4 179.5 50.5 109.2 23.7 0 0.0 226 -2.6 0 0.0 0 0.0 10 48
223 A 230 SER S H H X TS+ 0 0 -66.4 -38.3 179.2 49.0 110.6 29.2 219 -2.4 227 -1.8 0 0.0 0 0.0 8 44
224 A 231 GLU E H H X TS+ 0 0 -65.6 -52.5 179.8 44.0 114.1 13.1 220 -1.9 228 -2.6 0 0.0 0 0.0 9 53
225 A 232 ILE I H H X TS+ 0 0 -59.6 -38.3 180.0 57.2 109.6 27.8 221 -3.0 229 -2.5 0 0.0 0 0.0 9 63
226 A 233 ARG R H H X TS+ 0 0 -59.7 -40.1 -179.6 44.7 111.0 22.6 222 -2.6 230 -2.7 0 0.0 0 0.0 9 52
227 A 234 ALA A H H X TS+ 0 0 -70.8 -46.4 -179.9 46.8 113.8 23.7 223 -1.8 231 -1.3 0 0.0 0 0.0 12 48
228 A 235 LEU L H H < >TS+ 0 0 -67.2 -29.2 178.9 48.5 115.3 35.3 224 -2.6 233 -2.0 0 0.0 234 -1.3 12 56
229 A 236 THR T H H < >5TS+ 0 0 -73.8 -52.0 -179.8 52.1 107.9 14.5 225 -2.5 232 -2.4 0 0.0 0 0.0 12 65
230 A 237 ALA A H H < 35TS+ 0 0 -55.9 -24.7 179.7 48.3 112.6 41.9 226 -2.7 0 0.0 0 0.0 0 0.0 11 60
231 A 238 ASN N T h < 35TS- 0 0 -94.3 -7.9 177.2 -111.9 118.3 59.1 227 -1.3 41 -2.7 0 0.0 0 0.0 11 60
232 A 239 GLY G T T <5TS+ 0 0 95.5 2.4 179.9 113.1 87.5 63.4 229 -2.4 0 0.0 0 0.0 0 0.0 11 59
233 A 240 PHE F S e T - 0 0 -78.9 90.6 -179.4 -173.8 45.5 127.4 0 0.0 240 -1.9 0 0.0 0 0.0 11 45
238 A 245 ILE I T T 3 TS+ 0 0 -59.2 -20.7 180.0 66.3 77.9 48.8 236 -1.6 0 0.0 0 0.0 0 0.0 12 46
239 A 246 TYR Y T T 3 TS+ 0 0 -83.6 3.6 179.2 57.0 100.7 65.5 0 0.0 0 0.0 0 0.0 0 0.0 7 36
240 A 247 LYS K S t < TS- 0 0 -130.2 145.0 178.9 -93.8 104.9 161.2 237 -1.9 0 0.0 0 0.0 0 0.0 9 33
241 A 248 SER S g > T - 0 0 -57.4 145.7 -179.6 -123.8 33.1 110.4 0 0.0 244 -1.1 0 0.0 0 0.0 12 31
242 A 249 PHE F G G > TS+ 0 0 -58.8 -42.0 179.5 56.4 112.6 23.2 0 0.0 245 -1.9 0 0.0 0 0.0 10 48
243 A 250 ARG R G G 3 TS+ 0 0 -62.7 -21.8 -179.9 50.6 105.7 44.2 0 0.0 0 0.0 0 0.0 0 0.0 7 40
244 A 251 ALA A G G < TS+ 0 0 -96.3 1.0 179.8 113.6 88.2 68.6 241 -1.1 0 0.0 0 0.0 0 0.0 8 29
245 A 252 ASN N g < T - 0 0 -74.9 143.5 176.3 -138.0 61.4 117.4 242 -1.9 203 -2.6 0 0.0 0 0.0 14 38
246 A 253 LYS K B B A > T - 202 0 -91.1 164.3 -179.9 -107.0 29.6 126.5 0 0.0 249 -1.5 0 0.0 0 0.0 11 37
247 A 254 CYS C G G > TS+ 0 0 -62.3 -33.2 179.5 60.6 120.7 29.1 201 -2.6 250 -1.6 0 0.0 0 0.0 14 44
248 A 255 ALA A G G 3 TS+ 0 0 -68.1 -14.5 179.1 66.5 93.6 50.5 199 -2.4 0 0.0 0 0.0 0 0.0 12 34
249 A 256 ASP D G G < TS+ 0 0 -78.9 -21.1 178.9 80.8 89.2 46.0 246 -1.5 0 0.0 0 0.0 0 0.0 8 40
250 A 257 LEU L g < T - 0 0 -87.9 149.3 -179.3 -162.0 63.6 127.1 247 -1.6 0 0.0 0 0.0 0 0.0 12 56
251 A 258 CYS C E E DF - 235 0 -132.2 120.0 -178.8 -160.0 7.5 174.0 235 -2.3 235 -2.3 0 0.0 0 0.0 9 49
252 A 259 ILE I E E DF - 234 0 -104.1 150.6 -179.9 -140.4 8.5 142.0 0 0.0 0 0.0 0 0.0 0 0.0 10 48
253 A 260 SER S e - 0 0 -104.7 176.7 -179.6 -126.0 18.6 116.5 233 -1.3 0 0.0 0 0.0 0 0.0 8 45
254 A 261 GLN Q + 0 0 -103.5 13.0 -178.8 150.7 54.2 82.4 0 0.0 0 0.0 0 0.0 0 0.0 6 35
255 A 262 VAL V - 0 0 -54.6 131.7 178.3 -176.3 21.2 102.5 0 0.0 0 0.0 0 0.0 0 0.0 7 39
256 A 263 VAL V - 0 0 -123.2 161.1 -178.7 -83.2 50.2 146.3 0 0.0 0 0.0 0 0.0 0 0.0 7 30
257 A 264 ASN N S S S+ 0 0 -48.4 0.6 177.6 109.4 98.4 64.0 0 0.0 259 -2.5 0 0.0 0 0.0 7 26
258 A 265 MET M + 0 0 -75.6 56.9 -179.6 120.9 40.6 106.9 0 0.0 0 0.0 0 0.0 0 0.0 5 21
259 A 266 GLU E 0 0 -122.6 71.3 -179.0 999.9 999.9 131.7 257 -2.5 0 0.0 0 0.0 0 0.0 6 30
260 A 267 GLU E 0 0 -122.6 999.9 999.9 999.9 999.9 128.0 0 0.0 0 0.0 0 0.0 0 0.0 3 19
1f1zA.pdb
1F1Z DNA BINDING PROTEIN MOL_ID: 1; MOL_ID: 1;
author author
Kabs/Sand TTHHHHHHTTTT GGG SS GGG SS EEEE TTTSSEEEE SHHHHHHHHHHHH TTEEEEEEEEE HHHHHHHHHHHT EETTE Kabs/Sand
chirality --++++++++-++++-+-++---+--+++---+----+---++-+---+-+-++++++++++++-++++---+-----++++++++++-+---+---+- chirality
bends S SSSSSS SS SSS SS SSS SS SSSSS SSSSSSSSSSSSS SS S SSSSSSSSSSS SSS bends
turns TTTTTTTTTTTTTT TTTTT TTTTT TTTTT TTTTTTTTTTTTTTTTT TTTTTTTTTTTTTT TTTT turns
5-turns >5555< >5555< 5-turns
3-turns >>><<< >33X33< >>3<< >>3<< >33< >33< >33< 3-turns
bridge-2 bridge-2
bridge-1 AAAA AAAA B*BBBB*BB CC C bridge-1
sheets AAAA AAAA BBBBBBBBB CC C sheets
4-turns >>>><<<< >444< >>>>XXXXXX<<<< >>>>XXXXX<<<< 4-turns
summary tThHHHHHHhTTTt gGGGg SSgGGGg SS EEEEeTTTteEEEE hHHHHHHHHHHHHhTeEEEEEEEEEehHHHHHHHHHHHht eEETTE summary
sequence FSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRSHRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVIRGV sequence
10 20 30 40 50 60 70 80 90 100
author author
Kabs/Sand E EEEEEEEESSSSSSEEEEEE GGGGGSHHHHHHHHHHHHHHHHTT EEEE GGGS HHHHHHHHHHTS HHHHHTHHHHHHHHHHTTT Kabs/Sand
chirality ------+------+-+-++----+--+++++-++++++++++++++++-+-----+-+++--+++++++++++-+-++-+-+++++++++++++++++++ chirality
bends S SSSSSS SSSSSSSSSSSSSSSSSSSSSSS SSSS SSSSSSSSSS S SSSSSSSSSSSSSSSSS S bends
turns TTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTT turns
5-turns >5555< 5-turns
3-turns >>>X<<< >>3<< >>3<< >33< >33X>><<< >3><3 3-turns
bridge-2 DDDD eeee bridge-2
bridge-1 C BB*BBBBB DDDD eeee bridge-1
sheets C BBBBBBBB BBBBBB BBBB sheets
4-turns >444< >>>>XXXXXXXXXX<<<< >>>>XXXX<<<< >>>4<>>XXXX<<<< 4-turns
summary Ee eEEEEEEEEeSSSSeEEEEEEegGGGGGhHHHHHHHHHHHHHHHHhTteEEEEgGGGghHHHHHHHHHHht hHHHHHhHHHHHHHHHHhTT summary
sequence DQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPWFIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGD sequence
110 120 130 140 150 160 170 180 190 200
author author
Kabs/Sand SBHHHHHHHHHHHHT TTHHHHHHHHHHHTTSEE TTS GGG BGGG EE S Kabs/Sand
chirality --+++++++++++-+--+++++++++++++-++-+--++--+++--+++----+--++ chirality
bends S SSSSSSSSSSSS SSSSSSSSSSSSSSSSS SSS SSS SSS S bends
turns TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTT turns
5-turns >5555< >5555< 5-turns
3-turns < >33< >33< >33< >33<>>3<<>>3<< 3-turns
bridge-2 bridge-2
bridge-1 A FF A FF bridge-1
sheets DD DD sheets
4-turns >>>>XXXXXX<<<< >>>>XXXXX<<<< 4-turns
summary thHHHHHHHHHHHHhttThHHHHHHHHHHHhTeEE tTTtgGGGgBGGGgEEe S summary
sequence ENIINVCKQVDIAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEE sequence
210 220 230 240 250 260