HEADER LYASE COMPND MOL_ID: 1; COMPND 2 MOLECULE: MALATE SYNTHASE G; COMPND 3 CHAIN: A; COMPND 4 EC: 4.1.3.2; COMPND 5 ENGINEERED: YES; COMPND 6 MUTATION: YES; COMPND 7 OTHER_DETAILS: C-TERMINAL HIS-TAG (724-731) SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI; SOURCE 3 ORGANISM_COMMON: BACTERIA; SOURCE 4 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 5 EXPRESSION_SYSTEM_COMMON: BACTERIA; SOURCE 6 EXPRESSION_SYSTEM_PLASMID: PET-28B RESOLUTION SOURCE : ALS AUTHOR B.R.HOWARD,J.A.ENDRIZZI,S.J.REMINGTON RESOLUTION : TRONRUD,TEN EYCK,MATTHEWS EXPDTA X-RAY DIFFRACTION REVDAT 2 02-APR-00 1D8C 1 JRNL JRNL AUTH B.R.HOWARD,J.A.ENDRIZZI,S.J.REMINGTON JRNL TITL CRYSTAL STRUCTURE OF ESCHERICHIA COLI MALATE JRNL TITL 2 SYNTHASE G COMPLEXED WITH MAGNESIUM AND GLYOXYLATE JRNL TITL 3 AT 2.0 ANGSTROMS RESOLUTION: MECHANISTIC JRNL TITL 4 IMPLICATIONS JRNL REF BIOCHEMISTRY V. 39 3156 2000 JRNL REFN ASTM BICHAW US ISSN 0006-2960 RESOLUTION 2.00 ANGSTROMS.

Sequence in PIR format:

>P1;1d8cA structureX:1d8cA: 3 :A: 722 :A:malate synthase g:escherichia coli: 9.99: 9.99 QTITQSRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVHDLAPENRQLLAERDRIQAALDEWHRSNPGPVKD KAAYKSFLRELGYLVPQPERVTVETTGIDSEITSQAGPQLVVPANARYALNAANARWGSLYDALYGSDIIPQEGA /VSGYDPQRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVGYRGDAAAPTC ILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVAAVDAEDKILLYRNLLGLQGTLQ/RKLN DDRHYTAADGSEISLHGRSLLFIRNVGHL/TIPVIWDSEGNEIPEGILDGVTGAIALYDLKVQKNSRTGSVYIVK PK/HGPQEVAFANKLFTRIETLG/APNTLK/GI/DEERRTSLNLRSCIAQARNRVAFINTGFLDRTGDEHSV/EA GP/LRKNQ/KSTPWIKAYERNNVLSGLFCGLRGKAQIGKG/WA/PDL/ADYSQKGDQLRAGANTAWVPSPTAATL HALHYHQTNVQSVQANIAQTEFNAEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCS KVPDIHNVAL/EDRATLRISSQHIANWLRHGILTKEQVQASLENAKVVDQQNAGDPAYRP/AGNFANSCAFKAAS DLIFLGVKQPNGYTEPLLHAWRLREKES*

View SSTRUC file for 1d8cA

Rasmol View of 1d8cA