HEADER MERCURY DETOXIFICATION COMPND MOL_ID: 1; COMPND 2 MOLECULE: MERP; COMPND 3 CHAIN: NULL; COMPND 4 SYNONYM: MERCURIC TRANSPORT PROTEIN; COMPND 5 ENGINEERED: MERP GENE AMPLIFIED USING PCR; COMPND 6 BIOLOGICAL_UNIT: MONOMER; COMPND 7 OTHER_DETAILS: THE PROTEIN STUDIED EXCLUDES THE PRECURSOR COMPND 8 SIGNAL SEQUENCE. THIS IS THE NATIVE FORM OF THE PROTEIN COMPND 9 AFTER CELL PROCESSING. SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: SHIGELLA FLEXNERI; SOURCE 3 CELLULAR_LOCATION: PERIPLASM; SOURCE 4 PLASMID: R100 (NR1); SOURCE 5 GENE: MERP; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 7 EXPRESSION_SYSTEM_STRAIN: BL21; SOURCE 8 EXPRESSION_SYSTEM_VARIANT: DE3; SOURCE 9 EXPRESSION_SYSTEM_CELLULAR_LOCATION: CYTOPLASM; SOURCE 10 EXPRESSION_SYSTEM_VECTOR_TYPE: MALTOSE BINDING PROTEIN SOURCE 11 FUSION SYSTEM; SOURCE 12 EXPRESSION_SYSTEM_VECTOR: MALTOSE BINDING PROTEIN FUSION SOURCE 13 PROTEIN (PMAL-C2); SOURCE 14 EXPRESSION_SYSTEM_PLASMID: PSSS; SOURCE 15 EXPRESSION_SYSTEM_GENE: MERP; SOURCE 16 OTHER_DETAILS: MER GENES LOCATED ON TRANSPOSON TN21 AUTHOR R.A.STEELE,S.J.OPELLA RESOLUTION : BRUNGER EXPDTA NMR, 20 STRUCTURES JRNL AUTH R.A.STEELE,S.J.OPELLA JRNL TITL STRUCTURES OF THE REDUCED AND MERCURY-BOUND FORMS JRNL TITL 2 OF MERP, THE PERIPLASMIC PROTEIN FROM THE BACTERIAL JRNL TITL 3 MERCURY DETOXIFICATION SYSTEM JRNL REF BIOCHEMISTRY V. 36 6885 1997 JRNL REFN ASTM BICHAW US ISSN 0006-2960 0033 RESOLUTION L CITATION ABOVE. IN SUMMARY, INITIAL RESOLUTION NOT APPLICABLE.
Sequence in PIR format:
>P1;1afi- structureX:1afi-: 1 : : 72 : :merp:shigella flexneri: 9.99: 9.99 ATQTVTLAVPGMTCAACPITVKKALSKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ*
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