HEADER COMPLEX (NUCLEAR PROTEIN/RNA) COMPND MOL_ID: 1; COMPND 2 MOLECULE: U2 RNA HAIRPIN IV; COMPND 3 CHAIN: Q, R; COMPND 4 FRAGMENT: U2 HAIRPIN IV; COMPND 5 ENGINEERED: YES; COMPND 6 OTHER_DETAILS: 24 NUCLEOTIDE STEM-LOOP, U2 SNRNP HAIRPIN COMPND 7 IV; COMPND 8 MOL_ID: 2; COMPND 9 MOLECULE: U2 A'; COMPND 10 CHAIN: A, C; COMPND 11 FRAGMENT: RESIDUES 1 - 176; COMPND 12 ENGINEERED: YES; COMPND 13 MUTATION: C89D, S119C; COMPND 14 OTHER_DETAILS: THIS CONSTRUCT CONSISTS OF RESIDUES 1 - 176 COMPND 15 OF U2 A', WHICH IS A COMPONENT OF U2 SNRNP; COMPND 16 MOL_ID: 3; COMPND 17 MOLECULE: U2 B''; COMPND 18 CHAIN: B, D; COMPND 19 FRAGMENT: N-TERMINAL DOMAIN, RESIDUES 4 - 99; COMPND 20 ENGINEERED: YES; COMPND 21 OTHER_DETAILS: THIS CONSTRUCT CONSISTS OF RESIDUES 5 - 99 COMPND 22 OF U2 B DOUBLE PRIME, WHICH IS A COMPONENT OF U2 SNRNP SOURCE MOL_ID: 1; SOURCE 2 SYNTHETIC: YES; SOURCE 3 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 4 ORGANISM_COMMON: HUMAN; SOURCE 5 MOL_ID: 2; SOURCE 6 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 7 ORGANISM_COMMON: HUMAN; SOURCE 8 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 9 EXPRESSION_SYSTEM_STRAIN: BL21 (DE3); SOURCE 10 EXPRESSION_SYSTEM_CELLULAR_LOCATION: CYTOPLASM; SOURCE 11 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 12 EXPRESSION_SYSTEM_PLASMID: PET3; SOURCE 13 OTHER_DETAILS: CDNA CLONE; SOURCE 14 MOL_ID: 3; SOURCE 15 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 16 ORGANISM_COMMON: HUMAN; SOURCE 17 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 18 EXPRESSION_SYSTEM_STRAIN: BL21 (DE3); SOURCE 19 EXPRESSION_SYSTEM_CELLULAR_LOCATION: CYTOPLASM; SOURCE 20 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 21 EXPRESSION_SYSTEM_PLASMID: PET3; SOURCE 22 OTHER_DETAILS: CDNA CLONE RESOLUTION SOURCE : ELETTRA AUTHOR S.R.PRICE,P.R.EVANS,K.NAGAI RESOLUTION : MURSHUDOV,VAGIN,DODSON EXPDTA X-RAY DIFFRACTION JRNL AUTH S.R.PRICE,P.R.EVANS,K.NAGAI JRNL TITL CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B''-U2A' JRNL TITL 2 PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL JRNL TITL 3 NUCLEAR RNA JRNL REF NATURE V. 394 645 1998 JRNL REFN ASTM NATUAS UK ISSN 0028-0836 0006 RESOLUTION 2.38 ANGSTROMS.
Sequence in PIR format:
>P1;1a9nA structureX:1a9nA: 2 :A: 163 :A:u2 b'':homo sapiens: 9.99: 9.99 VKLTAELIEQAAQYTNAVRDRELDLRGYKIPVIENLGATLDQFDAIDFSDNEIRKLDGFPLLRRLKTLLVNNNRI CRIGEGLDQALPDLTELILTNNSLVELGDLDPLASLKSLTYLCILRNPVTNKKHYRLYVIYKVPQVRVLDFQKVK LKERQEAEKMFK*
View SSTRUC file for 1a9nA
Rasmol View of 1a9nA