3UOS

crystal structure of release factor rf3 trapped in the gtp state on a rotated conformation of the ribosome (without viomycin)

• Total interactions analyzed: 595
• Total true interactions: 39
• Strongest Interaction:
  • Chains: 8-G
  • Int. Res.: 11
  • Norm. En. per Res.: -4.5329
• Hub Node: H(5)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
0-7	0.0	0.0	-0.006	-0.006	2	0	0	8	0	1	2
0-Q	0.0	0.0	-0.0059	-0.0059	6	0	0	12	0	0	0
0-S	0.0	-6.8119	-5.9949	-12.8068	20	0	0	393	0	5	11
2-X	0.0	11.8962	-80.8978	-69.0016	46	1	1	4094	0	11	16
4-R	-13.7072	3.8638	-67.3957	-77.2392	40	2	5	3486	0	8	19
4-U	0.0	0.0	-13.1815	-13.1815	11	1	0	609	0	0	6
4-W	0.0	-5.0001	-62.9936	-67.9937	49	1	8	3629	2	11	9
5-7	0.0	1.0853	-22.7661	-21.6808	18	1	0	1064	0	1	8
6-E	0.0	0.0	-0.0039	-0.0039	3	0	0	5	0	0	0
6-X	0.0	0.0	-0.3221	-0.3221	9	0	0	118	0	1	3
7-P	-2.2103	14.5831	-186.733	-174.3602	59	10	5	8353	0	4	33
8-G	-18.6522	-21.1071	-10.1022	-49.8614	11	3	0	472	1	2	4
8-Q	0.0	0.0	-0.0191	-0.0191	2	0	0	16	0	0	1
D-N	0.0	4.3677	-23.3543	-18.9866	12	2	1	880	0	0	7
D-O	-9.5437	-8.862	-31.2797	-49.6855	19	4	0	1097	0	2	6
D-R	-4.9669	3.4131	-19.3505	-20.9043	16	0	0	1005	0	0	9
D-T	-14.567	6.6126	-144.42	-152.3743	57	8	6	6578	2	11	12
E-P	-5.6136	0.0	-105.214	-110.8276	47	6	5	5399	1	7	7
E-U	0.0	0.0	-0.3941	-0.3941	7	0	0	109	0	0	2
H-I	0.0	0.0	-23.6006	-23.6006	35	1	5	1239	0	12	8
H-J	0.0	14.8287	-43.8295	-29.0008	32	1	3	2377	0	8	5
H-K	-6.5772	-43.993	-70.2872	-120.8575	48	6	1	2800	4	4	9
H-L	0.0	0.1222	-117.432	-117.3098	41	6	7	5146	0	12	5
H-M	0.0	-13.5756	-62.7515	-76.3271	40	2	1	3011	1	10	7
I-Q	0.0	0.0	-0.0003	-0.0003	2	0	0	1	0	0	0
J-K	-16.4677	-3.4354	-70.7889	-90.692	45	11	3	3810	1	11	12
J-L	0.0	0.0	-2.5016	-2.5016	6	0	0	229	0	1	0
J-M	-9.9877	0.0	-55.8544	-65.8421	25	13	2	2163	0	0	2
K-M	0.0	-0.838	-22.9753	-23.8133	13	6	1	1074	0	2	1
L-M	0.0	-4.4589	-90.3276	-94.7865	53	4	2	4354	0	13	16
N-U	0.0	0.0	-81.5331	-81.5331	47	6	5	4464	0	8	22
N-V	0.0	0.0	-7.2716	-7.2716	12	0	0	567	0	0	4
O-T	-80.7271	-59.4407	-178.869	-319.0369	73	12	3	7041	4	29	30
P-U	0.0	0.0	-0.0046	-0.0046	7	0	0	9	0	0	2
P-V	0.0	2.6718	-37.573	-34.9012	22	0	1	1360	0	0	12
Q-Z	-8.7424	-2.8357	-87.7574	-99.3355	50	7	4	3857	0	4	12
R-T	0.0	0.0	-0.0026	-0.0026	2	0	0	3	0	0	0
R-W	0.0	0.0	-9.2891	-9.2891	11	1	0	318	0	2	2
U-V	-24.5553	-7.1192	-246.383	-278.0575	100	28	8	10619	2	21	48

Interface residues

• 0-7: ARG540, LYS387
• 0-Q: GLY60, GLY70, GLY85Q, LYS11Q, LYS86Q, SER80
• 0-S: ALA460, ALA480, ARG13S, ARG16S, ARG400, ASN490, CYS520, GLN19S, GLU20S, GLY470, GLY510, HIS450, LEU21S, LYS17S, LYS610, LYS710, PHE780, PRO730, THR12S, VAL500
• 2-X: ALA13X, ALA322, ALA332, ALA35X, ALA46X, ARG12X, ARG232, ARG292, ARG472, ARG6X, ASN272, GLN252, GLN312, GLN362, GLU172, GLU4X, GLU5X, GLY352, HIS15X, ILE30X, LEU11X, LEU142, LEU182, LEU192, LEU212, LEU222, LEU32X, LEU50X, LEU572, LEU7X, LEU8X, LYS33X, LYS49X, LYS542, LYS9X, MET1X, MET302, PHE262, PHE51X, PRO14X, SER342, VAL10X, VAL16X, VAL34X, VAL47X, VAL502
• 4-R: ALA111R, ALA444, ALA554, ARG118R, ARG394, ARG494, ARG514, ARG96R, CYS100R, GLU114R, GLU120R, GLY101R, GLY464, HIS31R, HIS374, HIS404, HIS414, ILE113R, ILE33R, ILE34R, ILE424, ILE544, ILE97R, LEU384, LEU38R, LEU98R, LYS35R, LYS524, LYS564, LYS99R, MET110R, PHE102R, PRO109R, SER119R, THR434, TYR112R, TYR474, TYR484, VAL116R, VAL534
• 4-U: ALA26U, ARG29U, ARG94, GLY25U, LYS114, LYS64, MET144, SER104, THR84, TYR24U, VAL30U
• 4-W: ALA14W, ALA204, ALA21W, ALA234, ALA43W, ALA444, ALA44W, ARG124, ARG154, ARG164, ARG18W, ARG394, ASN40W, ASP194, ASP22W, ASP34W, GLN15W, GLN31W, GLU354, GLU78W, GLY134, HIS374, HIS414, ILE24W, ILE35W, ILE424, LEU19W, LEU214, LEU23W, LEU274, LEU33W, LEU36W, LEU384, LYS16W, LYS27W, LYS364, LYS41W, SER264, SER284, SER30W, THR224, THR254, THR37W, THR39W, THR434, TYR38W, VAL17W, VAL20W, VAL244
• 5-7: GLU65, HIS185, HIS307, ILE317, ILE85, LEU327, LYS347, LYS357, LYS407, LYS75, LYS95, PHE195, THR215, THR225, THR235, THR337, THR377, TYR205
• 6-E: GLY54E, LYS376, THR53E
• 6-X: ALA406, ARG416, ARG73X, ARG76X, ASP79X, ILE74X, LYS36X, LYS466, LYS64X
• 7-P: ALA107, ALA267, ALA477, ALA597, ALA97, ARG127, ARG297, ARG47P, ARG48P, ARG59P, ARG60P, ARG77, ASN277, ASP537, CYS607, GLN54P, GLU51P, GLY49P, GLY527, GLY52P, GLY557, GLY65P, GLY87, HIS237, HIS257, ILE37, ILE587, LEU287, LEU547, LEU567, LEU57P, LEU617, LEU61P, LYS117, LYS147, LYS157, LYS227, LYS247, LYS27, LYS467, LYS47, LYS63P, MET487, MET55P, PHE137, PHE217, PHE50P, PHE64P, PHE66P, PRO56P, PRO627, PRO62P, SER507, THR57, TYR58P, TYR637, VAL497, VAL577, VAL67
• 8-G: ALA298, ARG169G, ASN138, CYS278, GLU308, LYS171G, LYS328, PRO155G, PRO318, SER288, VAL167G
• 8-Q: LYS118Q, LYS88
• D-N: GLY158D, GLY79N, GLY82N, GLY83N, HIS77N, HIS80N, ILE81N, LYS154D, LYS157D, LYS159D, PHE156D, THR78N
• D-O: ARG128D, ARG13D, ARG31O, ARG70O, ARG71O, ASP18D, ASP73O, GLY19D, GLY74O, HIS29O, ILE14D, ILE22D, PHE15D, PRO23D, PRO72O, SER21D, SER75O, THR16D, VAL20D
• D-R: ALA119D, ALA196D, ARG2R, ARG4R, ASN167D, GLY115D, GLY117D, GLY166D, HIS3R, LYS114D, LYS116D, LYS123D, MET165D, MET1R, PHE118D, THR197D
• D-T: ALA57T, ARG13D, ARG179D, ARG184D, ARG52T, ASN185D, ASN2T, ASP181D, ASP18D, GLN11T, GLN6T, GLN74T, GLU10T, GLU17D, GLU28D, GLU8T, GLY10D, GLY19D, GLY53T, HIS55T, HIS76T, ILE14D, ILE22D, ILE27D, ILE3T, ILE4T, LEU186D, LEU187D, LEU188D, LEU54T, LEU7T, LYS13T, LYS5T, LYS8D, MET11D, MET12T, PHE15D, PHE73T, PRO23D, PRO78T, SER1T, SER21D, SER56T, SER77T, THR12D, THR16D, THR197D, THR25D, THR75T, TRP30T, VAL20D, VAL24D, VAL26D, VAL32T, VAL79T, VAL80T, VAL9D
• E-P: ALA104E, ALA182E, ALA26E, ALA36E, ALA9P, ARG114E, ARG117E, ARG21P, ARG2P, ASN24E, ASN4P, ASP184E, GLN115E, GLN30E, GLN94E, GLU10P, GLU111E, GLU25E, HIS29E, ILE108E, ILE181E, LEU109E, LEU112E, LEU180E, LEU27E, LEU3P, LEU6P, LYS13P, PHE183E, PHE23E, PHE85E, PRO177E, PRO8P, SER107E, SER110E, SER12P, SER179E, SER42P, SER7P, THR5P, TYR35E, VAL113E, VAL178E, VAL28E, VAL31E, VAL32E, VAL33E
• E-U: ALA1U, ALA87E, ARG2U, ARG40E, ARG44E, GLN46E, VAL83E
• H-I: ALA113I, ALA114I, ALA119I, ALA123I, ALA1I, ALA44H, ALA48H, ARG126I, ARG42H, ARG46H, ARG53H, ASP115I, ASP120I, GLU122I, GLU40H, GLU47H, GLY118I, GLY45H, GLY49H, ILE121I, LEU41H, LYS2I, LYS37H, LYS3I, LYS43H, LYS71I, MET116I, MET38H, MET52H, PHE106H, THR117I, TYR51H, VAL33H, VAL35H, VAL50H
• H-J: ALA135H, ALA141H, ALA146H, ALA148H, ALA25J, ARG138H, ASP18J, GLU133H, GLU145H, GLU21J, GLU27J, ILE136H, ILE23J, ILE8J, LEU139H, LEU22J, LYS144H, LYS150H, LYS29J, MET140H, MET143H, MET14J, MET26J, PHE30J, SER147H, SER15J, SER24J, THR142H, VAL11J, VAL16J, VAL19J, VAL20J
• H-K: ALA10K, ALA127H, ALA12K, ALA135H, ALA137H, ALA13K, ALA141H, ALA146H, ALA148H, ALA25K, ALA91H, ARG138H, ARG153H, ASP18K, GLU133H, GLU134H, GLU145H, GLU21K, GLU28K, GLY149H, ILE23K, ILE7K, LEU126H, LEU129H, LEU139H, LEU151H, LEU22K, LYS144H, LYS150H, LYS29K, MET140H, MET143H, MET14K, MET17K, MET26K, PHE30K, SER147H, SER15K, THR128H, THR131H, THR142H, THR154H, TYR132H, VAL11K, VAL152H, VAL16K, VAL19K, VAL20K
• H-L: ALA10L, ALA13L, ALA148H, ALA156H, ALA157H, ALA161H, ALA25L, ARG153H, ARG159H, ASP160H, ASP18L, GLN6L, GLU21L, GLU27L, GLU28L, GLY149H, ILE23L, ILE7L, LEU151H, LEU155H, LEU22L, LYS144H, LYS150H, LYS162H, LYS29L, MET140H, MET143H, MET14L, MET17L, MET26L, PHE30L, SER147H, SER15L, SER24L, THR154H, VAL11L, VAL152H, VAL158H, VAL16L, VAL19L, VAL20L
• H-M: ALA10M, ALA12M, ALA13M, ALA146H, ALA148H, ALA156H, ALA157H, ALA161H, ALA25M, ARG153H, ASP160H, ASP18M, GLN6M, GLU145H, GLU163H, GLU21M, GLU28M, GLY149H, ILE23M, ILE7M, LEU151H, LEU155H, LEU22M, LYS144H, LYS150H, LYS29M, MET14M, MET17M, MET26M, PHE30M, SER147H, SER15M, SER24M, THR142H, THR154H, VAL11M, VAL152H, VAL158H, VAL16M, VAL19M
• I-Q: ARG59Q, LYS94I
• J-K: ALA10J, ALA10K, ALA12J, ALA13J, ALA13K, ALA25J, ALA25K, ASP18J, ASP18K, ASP5J, ASP5K, GLN6J, GLN6K, GLU21J, GLU21K, GLU28K, GLU9J, GLU9K, ILE23J, ILE2J, ILE2K, ILE7J, ILE7K, ILE8J, ILE8K, LEU22J, LEU22K, LYS29K, LYS4J, LYS4K, MET14K, MET17J, MET17K, MET26J, SER1J, SER1K, SER24J, SER24K, THR3J, THR3K, VAL11J, VAL11K, VAL16J, VAL20J, VAL20K
• J-L: ASP18J, LYS29L, MET14J, SER15J, VAL16J, VAL19J
• J-M: ALA10J, ALA10M, ALA12J, ALA12M, ALA13J, ALA13M, ASP18J, ASP18M, GLU9J, GLU9M, ILE7M, ILE8J, ILE8M, MET14J, MET14M, MET17J, MET17M, SER15J, SER15M, VAL11J, VAL11M, VAL16J, VAL16M, VAL19J, VAL19M
• K-M: ASP18M, GLU27K, GLU28K, ILE23M, LEU22M, LYS29K, MET14M, MET17M, PHE30K, SER15M, VAL16M, VAL19M, VAL20M
• L-M: ALA10L, ALA10M, ALA12L, ALA12M, ALA13L, ALA13M, ALA25L, ALA25M, ASP18L, ASP18M, ASP5L, GLN6L, GLN6M, GLU21L, GLU21M, GLU27L, GLU27M, GLU28L, GLU28M, GLU9L, GLU9M, ILE23L, ILE23M, ILE2L, ILE2M, ILE7L, ILE7M, ILE8L, ILE8M, LEU22L, LEU22M, LYS29L, LYS29M, LYS4L, LYS4M, MET14L, MET14M, MET17L, MET17M, MET26L, MET26M, SER1L, SER1M, SER24L, SER24M, THR3L, THR3M, VAL11L, VAL11M, VAL16M, VAL19M, VAL20L, VAL20M
• N-U: ALA42N, ALA62U, ALA66U, ALA67U, ALA68U, ALA6N, ALA95U, ALA98U, ARG34N, ARG35N, ARG37N, ARG57U, ARG63U, ARG69U, ARG91U, ASN65U, ASP96U, GLN55U, GLN58U, GLN70U, GLU31N, GLU43N, GLY38N, HIS40N, HIS47N, ILE64U, ILE93U, ILE97U, LEU59U, LYS102U, LYS12N, LYS2N, LYS39N, LYS41N, LYS7N, MET1N, PHE100U, PHE4N, PHE56U, PRO46N, THR3N, THR45N, THR5N, TRP60U, TYR16N, TYR44N, VAL99U
• N-V: ARG13V, ARG21V, GLN18V, HIS12V, LEU22V, LYS2N, MET1N, PHE4N, SER15V, THR19V, VAL14V, VAL20V
• O-T: ALA119O, ALA41T, ALA48T, ALA57T, ALA83O, ARG100T, ARG105O, ARG108O, ARG38T, ARG61T, ARG64O, ARG70O, ARG71O, ARG71T, ARG78O, ASN65T, ASN9O, ASP73O, ASP80O, GLN40T, GLN74T, GLU121O, GLU33T, GLU43T, GLU67T, GLU70T, GLY101O, GLY34T, GLY66T, GLY68O, GLY68T, GLY74O, GLY81O, HIS76T, ILE116O, ILE47T, ILE63T, ILE77O, ILE99O, LYS62T, LYS66O, MET113O, PHE100O, PHE42T, PHE58T, PHE73T, PHE79O, PRO102O, PRO120O, PRO72O, PRO78T, SER35T, SER64T, SER75O, SER77T, THR103T, THR104O, THR59T, THR65O, THR75T, TYR97T, VAL103O, VAL122O, VAL29T, VAL31T, VAL32T, VAL60T, VAL69O, VAL69T, VAL72T, VAL76O, VAL79T, VAL80T
• P-U: ARG12U, ARG18P, ARG21P, ARG5U, ILE23P, PHE35U, VAL3U
• P-V: ARG21P, ARG78V, ARG80V, ARG84V, GLY20P, GLY22P, GLY24P, GLY26P, GLY28P, GLY31P, GLY32P, HIS35P, HIS82V, ILE23P, LEU27P, LYS29P, LYS81V, LYS85V, PHE77V, SER25P, THR30P, TYR83V
• Q-Z: ALA21Q, ALA35Q, ALA52Z, ALA54Z, ALA74Z, ARG18Z, ARG38Q, ARG79Z, ASP25Q, ASP76Z, ASP90Z, GLN22Q, GLN51Z, GLN75Z, GLN78Z, GLN87Z, GLN97Q, GLU55Z, GLY19Q, GLY37Q, GLY99Q, HIS80Z, ILE89Z, LEU20Q, LEU61Z, LYS100Q, LYS127Q, LYS34Q, LYS53Z, LYS73Z, LYS83Z, LYS85Z, MET136Q, PHE130Q, PHE56Z, PHE91Z, PRO81Z, PRO84Z, PRO98Q, SER58Z, THR128Q, THR129Q, THR134Q, THR24Q, TYR57Z, TYR82Z, VAL131Q, VAL135Q, VAL36Q, VAL77Z
• R-T: ARG4R, SER1T
• R-W: ALA104R, ALA108R, ARG103R, ASN107R, ASN40W, GLY101R, LYS41W, PHE102R, PRO109R, THR39W, TYR38W
• U-V: ALA104U, ALA107U, ALA112U, ALA114U, ALA115U, ALA3V, ALA41U, ALA42V, ALA45U, ALA85U, ALA95U, ALA98U, ARG12U, ARG13V, ARG47U, ARG49U, ARG50U, ARG52U, ARG63U, ARG78V, ARG79V, ARG84V, ARG91U, ASN43V, ASP101U, ASP90U, ASP96U, GLN11V, GLN19U, GLN36U, GLN43U, GLN6V, GLN86V, GLN87V, GLU110U, GLU37V, GLU45V, GLU46V, GLU88U, GLY42U, GLY44V, GLY50V, GLY88V, GLY8V, GLY9V, HIS12V, HIS89V, ILE16U, ILE39U, ILE41V, ILE49V, ILE74V, ILE89U, ILE93U, ILE97U, LEU108U, LEU39V, LEU82U, LEU94U, LYS102U, LYS10V, LYS111U, LYS15U, LYS40U, LYS48V, LYS53U, LYS73V, LYS76V, LYS84U, LYS85V, LYS92U, MET1V, MET40V, PHE100U, PHE105U, PHE35U, PHE53V, PHE5V, PHE77V, PRO52V, SER7V, SER86U, THR106U, TYR2V, TYR31U, TYR44U, TYR46U, TYR83V, VAL103U, VAL109U, VAL14V, VAL38U, VAL47V, VAL4V, VAL51V, VAL54V, VAL72V, VAL75V, VAL87U, VAL99U