3UOQ

crystal structure of release factor rf3 trapped in the gtp state on a rotated conformation of the ribosome (without viomycin)

• Total interactions analyzed: 253
• Total true interactions: 24
• Strongest Interaction:
  • Chains: J-N
  • Int. Res.: 55
  • Norm. En. per Res.: -5.3675
• Hub Node: C(4)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
B-E	0.0	0.0	-0.122	-0.122	5	0	0	44	0	2	4
B-H	0.0	0.0	-0.1069	-0.1069	4	0	0	45	0	1	2
C-D	0.0	0.0	-0.073	-0.073	4	0	0	37	0	0	1
C-E	0.0	-11.1846	-6.2883	-17.4729	17	1	0	538	1	8	8
C-J	0.0	-0.6663	-68.3252	-68.9915	59	0	3	3207	0	13	7
C-N	0.0	-46.1888	-231.71	-277.8988	75	16	5	9515	3	35	33
D-E	0.0	-55.2803	-45.0708	-100.3511	43	3	0	2143	2	11	11
E-H	0.0	-7.2539	-84.3026	-91.5566	48	7	2	4033	0	16	13
F-R	-24.4094	-18.9768	-165.689	-209.0753	59	6	3	6537	2	12	27
G-I	0.0	4.2954	-20.198	-15.9026	32	1	0	1339	0	10	9
G-K	0.0	0.0	-0.5945	-0.5945	13	0	0	130	0	1	1
H-L	0.0	0.0	-9.9637	-9.9637	11	1	1	508	0	0	0
H-Q	0.0	0.0	-25.141	-25.141	17	0	0	1252	0	3	9
I-J	0.0	0.0	-24.2772	-24.2772	19	0	0	1239	0	8	12
I-N	0.0	0.0	-20.4122	-20.4122	9	0	0	557	0	0	2
J-N	-37.9618	-49.991	-207.262	-295.2148	55	14	5	8075	4	16	26
K-R	0.0	5.9137	-7.8035	-1.8897	17	0	0	691	0	5	10
K-U	-52.4897	53.6343	-222.494	-221.3494	76	16	2	9941	1	33	34
L-Q	0.0	0.0	-47.749	-47.749	21	0	4	2025	0	0	8
L-W	-31.599	-4.2695	-61.5732	-97.4417	55	39	2	4888	0	21	22
M-S	-3.7263	2.7702	-60.3672	-61.3233	42	8	0	3299	0	11	21
N-S	0.0	-2.7264	-27.2871	-30.0135	32	0	1	1960	0	5	10
O-Q	0.0	0.0	-0.1482	-0.1482	5	0	0	35	0	0	0
R-U	0.0	-5.9981	-25.5626	-31.5607	21	0	0	1440	0	14	9

Interface residues

• B-E: ARG68E, GLU64E, LEU178B, LYS104B, THR101B
• B-H: ASN20H, LYS21H, LYS25B, LYS27B
• C-D: ARG131C, ARG43D, ARG46D, LYS44D
• C-E: ALA57E, ARG130C, ARG163C, ARG168C, ARG19E, ARG53E, GLN138C, GLU169C, GLU54E, ILE29E, LYS134C, PHE30E, PHE32E, PRO56E, TRP166C, TYR167C, VAL55E
• C-J: ALA12J, ALA23C, ALA60C, ALA93J, ALA94J, ARG16J, ARG58C, ARG64C, ARG68J, ASN24C, ASP14J, ASP63J, ASP91J, ASP97J, GLN2C, GLN99C, GLN99J, GLU47J, GLU57C, GLU66J, GLY12C, GLY1C, GLY8C, GLY95J, HIS15J, HIS5C, HIS70J, ILE13C, ILE53J, ILE56C, ILE63C, ILE67J, ILE9C, LEU11C, LEU17J, LEU32C, LEU71J, LEU92J, LYS11J, LYS15C, LYS3C, PHE13J, PHE22C, PHE28C, PHE49J, PRO16C, PRO59C, SER19C, SER62C, THR20C, THR25C, THR69J, TRP17C, TRP21C, TYR65J, VAL14C, VAL4C, VAL96J, VAL98J
• C-N: ALA23C, ALA29C, ALA86N, ALA87N, ARG10C, ARG39C, ARG53C, ARG58C, ARG64N, ARG74N, ARG84N, ARG89N, ASN184C, ASN18C, ASN24C, ASN31C, ASN7C, ASP30C, ASP33C, ASP35C, GLN40C, GLN65N, GLU27C, GLU57C, GLU85N, GLU91N, GLY12C, GLY1C, GLY77N, GLY8C, GLY90N, GLY94N, HIS5C, ILE13C, ILE54C, ILE56C, ILE92N, ILE9C, LEU11C, LEU177C, LEU32C, LEU73N, LEU78N, LEU95N, LYS113C, LYS15C, LYS26C, LYS37C, LYS3C, LYS75N, LYS82N, LYS96N, LYS97N, MET88N, PHE22C, PHE28C, PHE36C, PHE72N, PHE76N, PRO16C, PRO6C, PRO93N, SER19C, SER34C, SER99N, THR185C, THR20C, THR25C, THR43C, TRP17C, TRP21C, TYR183C, VAL4C, VAL55C, VAL83N
• D-E: ALA106E, ALA109E, ALA112E, ALA78D, ALA79D, ARG111E, ARG46D, ASN84D, ASN88D, ASP49D, GLN53D, GLU100E, GLU115E, GLU196D, GLU201D, GLU87D, GLY101E, GLY103E, GLY108E, GLY83D, GLY86D, HIS120E, HIS197D, ILE104E, ILE105E, ILE199D, LEU198D, LEU202D, LEU81D, LYS13E, LYS205D, LYS82D, SER204D, SER48D, SER99E, THR102E, THR85D, TYR203D, TYR50D, TYR75D, VAL200D, VAL52D, VAL55E
• E-H: ALA129H, ALA153E, ALA154E, ALA69H, ALA96H, ARG113H, ARG156E, ASN145E, ASN147E, GLN66H, GLN81E, GLU144E, GLU150E, GLU41H, GLU42H, GLU46H, GLU72H, GLY157E, GLY43H, GLY97H, GLY99H, HIS82E, ILE100H, ILE45H, LEU98H, LYS155E, LYS158E, LYS40H, LYS63H, LYS68H, LYS85E, LYS93H, MET146E, MET151E, MET95H, PHE44H, PHE65H, PRO149E, PRO83E, PRO97E, SER148E, SER73H, TYR64H, VAL152E, VAL70H, VAL71H, VAL84E, VAL94H
• F-R: ALA26R, ALA48F, ALA57F, ALA57R, ALA61R, ALA99F, ARG38F, ARG45F, ARG56R, ARG60R, ARG62R, ARG72R, ARG86F, ARG91F, ASN63F, ASP21R, ASP24R, GLN46F, GLU40F, GLU5F, GLU98F, HIS55F, HIS73R, ILE20R, ILE25R, ILE51F, ILE6F, LEU47F, LEU61F, LEU64R, LEU66R, LEU67R, LYS23R, LYS56F, LYS59R, LYS93F, MET62F, MET88F, MET90F, MET9F, PHE8F, PRO50F, PRO68R, SER100F, SER65R, SER87F, THR70R, THR92F, THR97F, TRP42F, TYR22R, TYR49F, TYR59F, TYR63R, TYR69R, VAL60F, VAL7F, VAL89F, VAL96F
• G-I: ALA100I, ALA43I, ARG108I, ARG11I, ARG44I, ARG79I, ARG9G, ASP106I, ASP14G, GLN49I, GLU39G, GLU41I, GLU52I, GLY101I, ILE71I, LEU12G, LYS16G, LYS34G, LYS35G, MET45I, PHE102I, PHE17G, PRO13G, PRO15G, SER36G, SER40G, SER72I, THR37G, THR42I, TYR43G, VAL26G, VAL46I
• G-K: ALA146G, ALA149G, ARG97K, GLN63K, GLU93K, PHE150G, PHE60K, PRO59K, PRO90K, SER82G, SER94K, THR58K, TYR84G
• H-L: ALA1L, ARG79H, ARG83H, ASN4L, GLY81H, ILE84H, LEU82H, PRO80H, THR2L, VAL3L, VAL7L
• H-Q: ARG79H, ARG83H, ARG87H, GLU90H, GLY34Q, GLY81H, HIS30Q, ILE32Q, ILE84H, LEU82H, LYS29Q, LYS35Q, LYS86H, PHE36Q, PRO80H, TYR33Q, TYR85H
• I-J: ALA61J, ARG112I, ARG118I, ARG123I, ARG48J, ARG62J, ASP60J, ASP63J, GLN64J, GLY116I, LEU117I, LEU52J, LYS113I, LYS114I, LYS59J, PRO124I, THR50J, VAL115I, VAL51J
• I-N: ALA98N, ARG112I, GLU111I, LYS113I, LYS114I, SER99N, TRP100N, VAL110I, VAL115I
• J-N: ALA12J, ALA61J, ALA86N, ALA87N, ALA98N, ARG48J, ARG62J, ARG68J, ARG68N, ARG74N, ARG80N, ARG84N, ASN58J, ASP14J, ASP63J, GLN64J, GLU47J, GLU66J, GLU85N, GLY71N, GLY94N, GLY95J, HIS56J, HIS70J, HIS70N, ILE53J, ILE67J, ILE81N, ILE92N, LEU52J, LEU73N, LEU78N, LEU95N, LYS46J, LYS59J, LYS75N, LYS96N, LYS97N, MET88N, PHE13J, PHE49J, PHE72N, PHE76N, PRO55J, PRO93N, SER54J, SER79N, SER99N, THR50J, THR69J, TRP100N, TYR65J, VAL51J, VAL57J, VAL83N
• K-R: ARG62R, ARG72R, ASP111K, ASP71R, HIS117K, HIS21K, ILE115K, LYS37R, LYS86K, MET84K, PRO114K, SER35R, THR110K, THR113K, THR70R, TYR69R, VAL112K
• K-U: ALA101K, ALA14U, ALA24K, ALA25U, ALA29U, ARG105K, ARG121K, ARG126K, ARG127K, ARG16U, ARG20U, ARG32U, ARG33U, ARG34U, ARG6U, ARG92K, ARG97K, ASN100K, ASN108K, ASN118K, ASN27K, ASN8U, ASP111K, ASP12U, CYS120K, CYS22U, GLU23U, GLU30U, GLU35U, GLU7U, GLU82K, GLU93K, GLU9U, GLY119K, GLY26U, GLY87K, GLY89K, HIS117K, ILE106K, ILE109K, ILE115K, ILE22K, ILE3U, ILE96K, LEU15U, LEU28U, LYS124K, LYS125K, LYS19U, LYS24U, LYS39U, LYS4U, LYS86K, MET84K, PHE11U, PHE18U, PHE26K, PHE36U, PRO114K, PRO116K, PRO122K, PRO123K, PRO88K, PRO90K, THR107K, THR110K, THR113K, TYR37U, VAL112K, VAL128K, VAL13U, VAL27U, VAL31U, VAL5U, VAL83K, VAL85K
• L-Q: ALA12L, ARG11L, ARG39Q, ARG8L, ASN4L, GLN5L, GLY34Q, HIS30Q, ILE32Q, ILE37Q, LEU6L, LYS35Q, LYS38Q, LYS9L, PHE36Q, PRO10L, PRO31Q, THR2L, TYR33Q, VAL3L, VAL7L
• L-W: ALA100L, ALA123L, ARG120L, ARG30L, ARG400W, ARG402W, ARG487W, ASN433W, ASN434W, ASN489W, ASN72L, ASP102L, ASP405W, ASP435W, CYS103L, CYS33L, GLN409W, GLN411W, GLN492W, GLN74L, GLU464W, GLU484W, GLU490W, GLU75L, GLY105L, GLY31L, GLY99L, HIS76L, ILE431W, ILE79L, LEU101L, LEU403W, LEU407W, LEU493W, LEU73L, LEU80L, LYS119L, LYS122L, LYS404W, LYS408W, LYS410W, LYS486W, LYS488W, PHE485W, PRO121L, PRO406W, PRO430W, SER104L, SER432W, SER491W, SER77L, THR57L, VAL118L, VAL32L, VAL78L
• M-S: ALA74S, ARG100M, ARG2S, ARG35S, ARG77S, ARG78M, ARG80S, ARG86M, ARG89M, ARG91M, ARG92M, ASP63S, ASP81M, CYS84M, GLN99M, GLU64S, GLU72S, GLY67S, GLY71S, GLY83M, GLY87M, GLY98M, HIS68S, ILE39S, LEU79M, LEU82M, LEU88M, LEU94M, LYS69S, MET65S, PHE60S, PHE73S, PRO75S, THR101M, THR62S, THR76S, THR78S, TYR79S, TYR85M, VAL59S, VAL61S, VAL66S
• N-S: ALA28N, ALA35N, ALA43N, ARG36S, ARG40N, ARG52N, ASN34N, ASN42N, ASP11S, ASP32N, GLN48N, GLY7S, HIS13S, ILE10S, LEU12S, LEU15S, LEU45N, LEU47N, LEU4S, LYS27N, LYS46N, LYS5S, LYS6S, PHE40S, PHE9S, PRO8S, SER31N, SER34S, THR49N, TRP33S, VAL33N, VAL44N
• O-Q: ALA81Q, ARG71O, LYS70O, VAL74O, VAL82Q
• R-U: ALA51U, ARG42R, ARG72R, ASP71R, CYS22U, GLU23U, GLU34R, GLY26U, GLY36R, ILE3U, LYS19U, LYS37R, LYS48U, LYS4U, PHE18U, SER21U, SER35R, THR33R, THR70R, VAL27U, VAL5U