3OGO

structure of the gfp:gfp-nanobody complex at 2.8 a resolution in spacegroup p21212

• Total interactions analyzed: 28
• Total true interactions: 14
• Strongest Interaction:
  • Chains: C-F
  • Int. Res.: 73
  • Norm. En. per Res.: -4.4099
• Hub Node: B(5)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
A-B	-19.6859	-3.8942	-64.9304	-88.5105	48	3	0	3099	0	24	37
A-E	-4.5581	0.1301	-3.4665	-7.8944	16	2	0	338	2	6	7
A-F	0.0	0.0	-5.4031	-5.4031	10	1	0	315	0	3	1
B-C	-17.728	-3.4805	-67.3165	-88.525	52	4	0	2976	1	32	27
B-E	0.0	-0.6237	-1.5805	-2.2042	7	0	0	163	0	5	4
B-F	-2.5103	-5.5715	-8.8285	-16.9103	12	1	0	422	2	7	7
B-G	-38.2305	-63.3926	-222.992	-324.6151	74	13	1	7368	6	28	19
B-H	0.0	0.0	-12.0442	-12.0442	15	1	0	441	0	0	0
C-F	-40.0052	-80.0401	-201.876	-321.9213	73	13	1	6937	7	30	21
C-G	0.0	7.0487	-0.349	6.6996	5	0	0	62	0	4	11
C-H	-34.9672	-39.7326	-61.884	-136.5839	50	12	0	3047	2	13	8
D-E	-8.8763	-13.3745	-43.9391	-66.1899	46	9	0	2646	0	10	10
D-F	-9.9473	-34.2692	-22.3192	-66.5357	49	4	0	1762	2	13	11
E-F	-24.6451	13.3665	-150.707	-161.9856	79	10	0	6012	0	14	17

Interface residues

• A-B: ARG215A, ARG215B, ASN23A, ASN23B, ASP19A, ASP19B, ASP21A, ASP21B, CYS48A, CYS48B, GLU132A, GLU17A, GLU17B, GLY20A, GLY20B, GLY24A, GLY24B, GLY51A, GLY51B, HIS25A, HIS25B, ILE47A, ILE47B, LEU18A, LEU18B, LEU53A, LEU53B, LYS214B, LYS26A, LYS26B, LYS52A, LYS52B, PHE27A, PHE27B, PRO54A, PRO54B, SER28A, SER28B, SER30A, SER30B, THR49A, THR49B, THR50A, THR50B, VAL22A, VAL22B, VAL29A, VAL29B
• A-E: ALA55E, ARG215A, ARG32E, ARG36E, ASN100E, ASN212A, ASP210A, GLU32A, GLY102E, GLY33A, LYS45A, PRO211A, SER34E, SER54E, TYR33E, VAL101E
• A-F: ASP117A, GLN14F, GLU115A, GLY116A, PRO13A, PRO15F, PRO89F, SER115F, SER116F, THR118A
• B-C: ALA154B, ASN105B, ASN105C, ASN164B, ASN198B, ASN23C, ASP102B, ASP102C, ASP103C, ASP129C, ASP133C, ASP155B, ASP180B, GLN157B, GLN183B, GLN184B, GLN184C, GLU132C, GLY104C, GLY134C, HIS181B, ILE128B, ILE128C, ILE98B, LEU137C, LEU178B, LYS101B, LYS101C, LYS107B, LYS107C, LYS126C, LYS131C, LYS140C, LYS156B, LYS158B, LYS158C, LYS162B, LYS162C, LYS166B, MET153B, PHE100B, PHE100C, PHE130C, PHE165B, PHE99B, PHE99C, THR97B, THR97C, TYR151B, TYR182B, TYR182C, VAL163B
• B-E: ARG58E, ASN23B, ASP133B, ASP57E, GLU132B, LYS131B, PHE130B
• B-F: ASN105B, ASP129B, ASP63F, GLU62F, GLY104B, ILE128B, LYS107B, LYS126B, LYS66F, PHE99B, SER64F, TYR61F
• B-G: ALA206B, ALA50G, ALA55G, ARG168B, ARG36G, ARG46G, ARG58G, ASN100G, ASN135B, ASN144B, ASN146B, ASN149B, ASN170B, ASN98G, ASP102B, ASP173B, ASP57G, ASP63G, GLN177B, GLN204B, GLN40G, GLU104G, GLU142B, GLU172B, GLU222B, GLU45G, GLU47G, GLU62G, GLY102G, GLY174B, GLY51G, GLY56G, HIS148B, HIS169B, ILE171B, ILE71G, LEU141B, LEU178B, LEU207B, LEU220B, LEU221B, LYS101B, LYS140B, LYS166B, LYS41B, MET35G, MET52G, PHE103G, PHE165B, PHE223B, SER147B, SER175B, SER205B, SER208B, SER34G, SER53G, SER54G, SER59G, SER60G, THR203B, TRP106G, TRP37G, TRP48G, TYR105G, TYR143B, TYR145B, TYR151B, TYR33G, TYR38G, TYR61G, VAL101G, VAL176B, VAL49G, VAL99G
• B-H: ALA154B, ASN198B, ASP197B, GLN2H, GLN4H, GLN80B, GLY27H, HIS81B, ILE229B, LEU195B, LYS156B, PRO196B, SER26H, TYR105H, VAL3H
• C-F: ALA206C, ALA50F, ALA55F, ARG168C, ARG36F, ARG46F, ARG58F, ASN100F, ASN135C, ASN144C, ASN146C, ASN149C, ASN164C, ASN170C, ASN98F, ASP102C, ASP173C, ASP180C, ASP57F, ASP63F, GLN177C, GLN204C, GLU104F, GLU142C, GLU172C, GLU222C, GLU45F, GLU47F, GLU62F, GLY102F, GLY174C, GLY51F, GLY56F, HIS148C, HIS169C, ILE171C, ILE71F, LEU141C, LEU178C, LEU207C, LEU220C, LEU221C, LYS101C, LYS140C, LYS166C, MET35F, MET52F, PHE103F, PHE165C, PHE223C, SER147C, SER175C, SER205C, SER208C, SER34F, SER53F, SER54F, SER59F, SER60F, THR203C, TRP106F, TRP37F, TRP48F, TYR105F, TYR143C, TYR145C, TYR33F, TYR38F, TYR61F, VAL101F, VAL176C, VAL49F, VAL99F
• C-G: ASN23C, GLU132C, GLU45G, HIS25C, LYS44G
• C-H: ALA110C, ALA11H, ALA24H, ARG109C, ARG122C, ARG20H, ARG77H, ASN159C, ASP117C, ASP190C, CYS23H, GLN108H, GLN111H, GLN14H, GLN157C, GLU111C, GLU115C, GLU124C, GLU7H, GLU90C, GLU95C, GLY109H, GLY10H, GLY116C, GLY189C, GLY191C, GLY9H, ILE188C, LEU12H, LEU15C, LEU19H, LEU21H, LYS113C, LYS117H, LYS158C, PHE114C, PRO13C, PRO187C, SER22H, SER8H, THR110H, THR113H, THR118C, THR186C, TYR81H, VAL112H, VAL120C, VAL13H, VAL6H, VAL93C
• D-E: ALA11E, ALA24E, ALA76E, ARG122D, ARG20E, ARG77E, ASN159D, ASN78E, ASP190D, ASP74E, CYS23E, GLN108E, GLN111E, GLN83E, GLU111D, GLU115D, GLU7E, GLU90D, GLY10E, GLY116D, GLY189D, GLY191D, GLY91D, GLY9E, ILE188D, LEU12E, LEU19E, LEU21E, LYS113D, LYS3D, MET84E, PHE114D, PRO187D, PRO192D, PRO89D, SER18E, SER22E, SER8E, THR110E, THR79E, TYR81E, VAL112D, VAL112E, VAL120D, VAL13E, VAL6E
• D-F: ALA110D, ALA11F, ALA24F, ARG109D, ARG122D, ARG20F, ARG77F, ASN159D, ASP190D, ASP19D, ASP74F, CYS23F, GLN108F, GLN111F, GLN14F, GLU111D, GLU115D, GLU124D, GLU17D, GLU7F, GLU90D, GLU95D, GLY109F, GLY10F, GLY189D, GLY191D, GLY9F, ILE123D, ILE188D, LEU12F, LEU19F, LEU21F, LYS113D, LYS126D, LYS26D, PRO187D, SER18F, SER22F, SER30D, SER8F, THR110F, THR186D, THR49D, THR79F, TYR81F, VAL120D, VAL13F, VAL6F, VAL93D
• E-F: ALA55E, ALA55F, ALA76E, ALA76F, ARG20E, ARG20F, ARG58E, ARG58F, ARG68E, ARG68F, ARG73E, ARG73F, ARG77E, ARG77F, ASN31E, ASN31F, ASN78E, ASN78F, ASN85E, ASN85F, ASP57E, ASP57F, ASP74E, ASP74F, ASP75E, ASP75F, GLN14E, GLN83E, GLN83F, GLY16E, GLY16F, GLY17E, GLY17F, GLY56E, GLY56F, GLY67E, GLY67F, GLY9E, GLY9F, ILE71E, ILE71F, LEU19E, LEU19F, LEU21E, LEU21F, LEU87E, LEU87F, LYS66E, LYS66F, MET52E, MET52F, MET84E, MET84F, PHE69E, PHE69F, SER18E, SER18F, SER22E, SER22F, SER59E, SER59F, SER72E, SER72F, SER86E, SER86F, THR70E, THR70F, THR79E, THR79F, TYR61E, TYR61F, TYR81E, TYR81F, VAL13E, VAL13F, VAL30E, VAL30F, VAL80E, VAL80F