3I8I

elongation complex of the 70s ribosome with three trnas and mrna. this entry 3i8i contains 50s ribosomal subnit. the 30s ribosomal subunit can be found in pdb entry 3i8h. molecule a in the same asymmetric unit is deposited as 3i8f (50s) and 3i8g (30s).

• Total interactions analyzed: 561
• Total true interactions: 30
• Strongest Interaction:
  • Chains: N-R
  • Int. Res.: 80
  • Norm. En. per Res.: -4.2338
• Hub Node: E(4)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
E-M	0.0	0.0	-36.9138	-36.9138	12	0	1	1296	0	0	2
E-N	-15.7565	6.0751	-33.7179	-43.3993	16	3	0	1280	0	8	9
E-0	0.0	6.7782	-14.2202	-7.4421	18	10	0	1192	0	0	8
E-R	0.0	-17.6816	-138.825	-156.5066	77	9	6	7333	4	19	17
F-O	-5.237	4.3689	-89.9705	-90.8385	46	12	3	4828	0	14	16
F-1	0.0	0.0	-1.0366	-1.0366	9	0	0	277	0	0	2
G-4	-6.3769	-5.5198	-226.865	-238.7616	108	71	4	13657	2	24	25
I-Y	-8.4748	1.0312	-28.2193	-35.6629	31	5	2	1324	0	4	5
I-J	0.0	51.7704	-9.1325	42.6379	31	25	3	2829	0	19	19
K-Z	-2.1508	0.0	-46.0301	-48.1809	33	2	1	2002	0	7	9
Y-L	-4.9881	-19.5423	-80.7512	-105.2816	31	2	0	3187	2	9	6
Y-J	-4.723	61.7259	32.1664	89.1693	60	76	8	8012	3	18	21
M-1	-36.9571	-5.0512	-123.387	-165.3953	50	15	1	6169	0	8	24
M-2	0.0	0.0	-13.5546	-13.5546	13	3	0	946	0	1	4
N-R	-59.6111	-64.6401	-214.451	-338.7022	80	33	5	9785	7	39	40
O-1	0.0	0.0	-0.0283	-0.0283	6	0	0	22	0	0	2
O-2	0.0	0.0	-11.7302	-11.7302	15	1	0	837	0	0	5
O-8	-13.4411	22.4674	-180.025	-170.9987	61	23	0	8268	0	18	64
P-V	-31.934	-150.3576	-148.671	-330.9626	103	34	5	8500	10	66	51
P-3	-9.8574	-21.8644	-2.8735	-34.5953	21	19	1	2034	2	3	8
0-S	0.0	-7.4769	-15.3527	-22.8296	19	1	0	779	0	5	9
0-5	-3.8373	10.0831	-79.4612	-73.2153	39	10	5	3928	0	6	15
Q-3	0.0	-4.6645	-4.5381	-9.2026	24	0	0	464	0	9	13
1-2	-22.3557	-31.6116	-234.794	-288.7612	91	43	11	11177	1	19	38
1-5	0.0	1.0337	-7.2053	-6.1716	14	0	0	562	0	0	10
S-5	0.0	39.1253	-96.9313	-57.806	58	11	4	5163	0	13	21
T-W	-15.6694	-12.6976	-105.047	-133.414	48	4	3	4597	2	8	11
T-7	0.0	2.2233	0.112	2.3353	9	3	0	147	0	3	10
3-8	0.0	0.0	-0.0277	-0.0277	2	0	0	16	0	2	1
6-8	0.0	0.0	-37.7951	-37.7951	17	9	0	1446	0	3	2

Interface residues

• E-M: ARG149E, GLY153E, GLY77M, GLY80M, LYS152E, LYS154E, PRO79M, SER76M, TYR151E, TYR75M, TYR78M, VAL150E
• E-N: ALA20E, ALA76N, ARG13E, ARG16E, ARG19E, ARG71N, ASP17E, ASP18E, ASP73N, GLY74N, LYS70N, PHE15E, PRO22E, PRO72N, SER75N, VAL21E
• E-0: ALA114E, ALA162E, ARG111E, ARG20, ASN192E, GLY110E, GLY112E, GLY115E, GLY161E, HIS30, LEU40, LYS109E, LYS118E, MET10, PHE113E, PRO191E, SER108E, TYR160E
• E-R: ALA20E, ALA28E, ALA5R, ARG13E, ARG13R, ARG16E, ARG16R, ARG19E, ARG3R, ARG53R, ASN180E, ASN192E, ASN2R, ASN55R, ASN58R, ASP174E, ASP17E, ASP18E, GLN54E, GLU11R, GLU178E, GLU179E, GLY10E, GLY193E, GLY194E, GLY29E, GLY4R, GLY6E, HIS79R, ILE14E, ILE176E, ILE26E, ILE75R, ILE7R, ILE83R, LEU181E, LEU182E, LEU183E, LEU27E, LEU52E, LEU6R, LEU78R, LEU82R, LEU9R, LYS33R, LYS35R, LYS8E, LYS8R, MET11E, MET1R, PHE15E, PHE51E, PHE57R, PHE76R, PRO177E, PRO53E, PRO77R, PRO81R, SER12R, SER80R, THR12E, THR24E, THR40R, THR59R, THR60R, TYR14R, TYR32R, VAL10R, VAL15R, VAL173E, VAL175E, VAL21E, VAL23E, VAL25E, VAL75E, VAL7E, VAL9E
• F-O: ALA113F, ALA12O, ARG117F, ARG119F, ARG15O, ARG188F, ARG38F, ARG44F, ARG7O, ARG95F, ASN13O, ASN29F, ASN9O, ASP116F, ASP185F, ASP5O, GLN40F, GLU120F, GLU190F, GLU35F, GLY11O, HIS31F, ILE186F, ILE28F, LEU32F, LEU33F, LEU3O, LEU41F, LEU6O, LYS17O, LYS2O, MET112F, MET1O, PHE90F, PRO10O, PRO30F, PRO8O, SER42O, SER4O, THR189F, TRP34F, TYR184F, VAL183F, VAL187F, VAL36F, VAL37F
• F-1: ALA49F, ARG31, ARG45F, GLY91F, PRO21, PRO92F, THR51F, TYR97F, VAL88F
• G-4: ALA110G, ALA124, ALA61G, ALA6G, ARG113G, ARG115G, ARG118G, ARG181G, ARG484, ARG96G, ARG98G, ARG9G, ASN108G, ASN204, ASP116G, ASP147G, ASP4G, ASP97G, CYS164, CYS184, CYS364, CYS394, GLN464, GLN58G, GLN66G, GLU104G, GLU143G, GLU234, GLU34, GLU344, GLU59G, GLY119G, GLY174, GLY177G, GLY194, GLY44, GLY65G, HIS404, HIS64, ILE101G, ILE114G, ILE140G, ILE144, ILE144G, ILE154, ILE224, ILE314, ILE54, ILE63G, LEU106G, LEU107G, LEU111G, LEU120G, LEU139G, LEU3G, LEU60G, LEU62G, LEU7G, LEU94, LEU94G, LYS105G, LYS10G, LYS182G, LYS24, LYS284, LYS384, LYS67G, LYS84, LYS8G, MET14, MET148G, MET99G, PHE102G, PHE117G, PHE141G, PHE178G, PHE180G, PHE424, PHE494, PRO112G, PRO114, PRO122G, PRO142G, PRO179G, PRO294, PRO2G, PRO414, PRO68G, PRO74, SER264, SER374, THR145G, THR244, THR274, THR444, THR64G, TRP100G, TYR146G, TYR254, TYR324, TYR434, VAL104, VAL109G, VAL214, VAL334, VAL354, VAL5G, VAL92G
• I-Y: ALA136Y, ALA144Y, ALA26I, ALA2I, ALA30I, ARG135Y, ASP25I, GLN115Y, GLN143Y, GLN22I, GLU10I, GLU133Y, GLU137Y, GLU19I, GLU29I, GLU6I, GLY140Y, ILE116Y, ILE24I, ILE5I, LEU138Y, LEU142Y, LEU18I, LEU23I, LEU27I, LYS21I, LYS28I, MET1I, PRO145Y, VAL139Y, VAL141Y
• I-J: ALA26I, ALA2I, ALA2J, ALA30I, ARG7I, ARG7J, ASP25I, ASP25J, ASP4J, GLN22J, GLU29I, GLU6I, GLU6J, ILE24I, ILE24J, ILE5I, ILE5J, LEU12J, LEU18I, LEU18J, LEU27I, LEU3I, LEU3J, LYS21I, LYS21J, LYS28I, LYS28J, LYS9J, MET1I, MET1J, VAL17J
• K-Z: ALA26K, ALA39K, ALA64Z, ARG27K, ARG50Z, ARG52Z, ARG76Z, ASN28K, ASN43K, GLU41K, GLU72Z, GLU75Z, GLY24K, ILE4K, ILE67Z, LEU31K, LEU38K, LEU44K, LEU73Z, LYS48Z, MET1K, PRO23K, PRO32K, PRO68Z, SER42K, THR40K, TYR29K, TYR71Z, VAL37K, VAL49Z, VAL51Z, VAL70Z, VAL74Z
• Y-L: ALA122L, ALA123L, ALA124L, ALA39Y, ALA43Y, ARG41Y, ASN116L, ASN47Y, ASP114L, ASP119L, GLN42Y, GLN46Y, GLU121L, GLU36Y, HIS38Y, ILE104Y, LEU115L, LEU120L, LEU40Y, LEU44Y, LYS35Y, LYS3L, LYS45Y, LYS70L, MET112L, MET126L, PRO113L, THR117L, THR118L, THR37Y, VAL5L
• Y-J: ALA124Y, ALA126Y, ALA136Y, ALA15J, ALA26J, ALA2J, ALA30J, ALA88Y, ALA89Y, ALA90Y, ARG135Y, ARG7J, ASP132Y, ASP25J, ASP4J, GLN115Y, GLN143Y, GLN14J, GLN22J, GLU10J, GLU11J, GLU133Y, GLU137Y, GLU19J, GLU29J, GLU6J, GLY140Y, ILE116Y, ILE24J, ILE5J, ILE8J, LEU117Y, LEU125Y, LEU12J, LEU134Y, LEU138Y, LEU142Y, LEU18J, LEU20J, LEU23J, LEU27J, LEU3J, LEU93Y, LYS21J, LYS91Y, LYS9J, MET131Y, PHE24Y, PRO129Y, PRO86Y, SER13J, THR118Y, THR130Y, THR16J, THR92Y, VAL139Y, VAL141Y, VAL17J, VAL87Y, VAL94Y
• M-1: ALA671, ALA681, ALA961, ALA991, ARG1011, ARG35M, ARG39M, ARG591, ARG641, ARG701, ARG921, ASN45M, ASN661, ASN941, ASP41M, ASP561, ASP911, ASP971, CYS691, GLN711, GLY36M, HIS38M, HIS721, ILE621, ILE651, LEU601, LEU951, LEU981, LYS2M, LYS37M, LYS7M, LYS931, MET1M, MET48M, PHE1061, PHE571, PRO1031, PRO40M, PRO44M, PRO6M, THR32M, THR3M, THR43M, TRP42M, TRP611, TYR4M, VAL1001, VAL46M, VAL5M, VAL631
• M-2: ALA32, ARG132, ARG212, GLN112, ILE42, LEU182, LEU202, LYS102, LYS2M, MET1M, PHE22, TYR122, VAL142
• N-R: ALA103N, ALA118N, ALA11N, ALA76N, ALA83N, ARG103R, ARG104N, ARG107N, ARG39R, ARG41R, ARG51R, ARG64N, ARG64R, ARG71N, ARG74R, ARG78N, ASN38R, ASN82N, ASP44R, ASP73N, ASP80N, ASP81N, GLN43R, GLU105N, GLU108N, GLU120N, GLU36R, GLU46R, GLU68N, GLU73R, GLU9N, GLY100N, GLY37R, GLY69R, GLY71R, GLY74N, ILE42R, ILE50R, ILE69N, ILE75R, ILE77N, ILE83R, LEU122N, LEU82R, LYS33R, LYS35R, LYS65R, LYS66N, LYS70N, MET112N, PHE45R, PHE61R, PHE76R, PHE79N, PHE99N, PRO101N, PRO119N, PRO72N, PRO77R, PRO81R, SER106R, SER67R, SER75N, SER80R, THR60R, THR62R, THR65N, TYR100R, TYR32R, TYR68R, VAL102N, VAL115N, VAL121N, VAL34R, VAL63N, VAL63R, VAL66R, VAL70R, VAL72R, VAL98N
• O-1: ARG21O, ARG361, GLY22O, LYS131, PHE401, PRO23O
• O-2: ARG21O, ARG822, ARG832, GLN802, GLY22O, GLY24O, GLY26O, GLY28O, LYS29O, LYS782, PRO23O, SER25O, THR30O, TYR812, VAL792
• O-8: ALA108, ALA248, ALA558, ARG138, ARG308, ARG468, ARG49O, ARG50O, ARG55O, ARG578, ARG61O, ARG65O, ASP47O, GLN68O, GLU52O, GLU548, GLU568, GLU658, GLY288, GLY458, GLY66O, GLY69O, GLY98, HIS318, HIS78, ILE168, ILE588, LEU508, LEU59O, LEU608, LEU618, LEU62O, LYS107O, LYS118, LYS128, LYS158, LYS268, LYS298, LYS448, LYS478, LYS528, LYS58, LYS598, LYS64O, LYS88, MET258, MET48, MET60O, MET67O, PHE51O, PRO48O, PRO538, PRO63O, THR278, THR57O, THR58O, THR68, VAL148, VAL228, VAL238, VAL498
• P-V: ALA136P, ALA140P, ALA184V, ALA187V, ALA188V, ALA189V, ALA20P, ALA28P, ALA36P, ALA51V, ARG101P, ARG122V, ARG133P, ARG134P, ARG49V, ARG56P, ARG59P, ARG60P, ARG67P, ARG72V, ARG79V, ARG80V, ARG81V, ARG82V, ASN75V, ASP123V, ASP135P, ASP138P, ASP179V, ASP25P, ASP31P, ASP45V, ASP77V, GLN118V, GLN141P, GLN50V, GLN73V, GLU105P, GLU119V, GLU139P, GLU178V, GLU181V, GLU185V, GLU186V, GLU190V, GLU38P, GLU84V, GLY100P, GLY19P, GLY23P, GLY24P, GLY30P, GLY33P, GLY61P, GLY62P, HIS121V, HIS54V, ILE124V, ILE131P, ILE53V, LEU125V, LEU17P, LEU183V, LEU24V, LEU37P, LEU41V, LEU76V, LYS130P, LYS182V, LYS18P, LYS22P, LYS46V, LYS63P, LYS78V, LYS98P, PHE29P, PHE44V, PHE48V, PHE58P, PHE65P, PRO101V, PRO176V, PRO39P, PRO83V, PRO99P, SER52V, THR21P, TYR137P, TYR26P, TYR99V, VAL100V, VAL102P, VAL132P, VAL165V, VAL180V, VAL27P, VAL35P, VAL42V, VAL47V, VAL55P, VAL71V, VAL74V, VAL86V
• P-3: ARG10P, ARG113, ARG82P, ASN123, GLU80P, GLY63, GLY83, GLY84P, LEU73, LEU79P, LYS11P, LYS43, LYS53, LYS77P, LYS85P, LYS87P, MET83P, PRO78P, SER93, THR103, VAL81P
• 0-S: ALA1010, ALA1090, ARG1030, ARG1040, ARG1050, ARG37S, ARG42S, ASN40S, ASP1070, GLU1020, GLY1060, GLY1080, LEU1000, LYS41S, PHE46S, PRO1100, THR39S, TYR38S, TYR45S
• 0-5: ALA1010, ALA1120, ALA535, ARG1030, ARG330, ARG555, ARG960, CYS465, GLU1020, GLU1150, GLU1180, GLU485, GLY505, GLY545, HIS435, LEU1000, LEU1110, LEU1130, LEU1160, LEU305, LEU585, LEU980, LYS405, LYS565, LYS990, MET395, PRO1100, PRO415, PRO425, PRO475, THR350, THR445, TYR515, TYR525, VAL1170, VAL380, VAL455, VAL575, VAL970
• Q-3: ARG20Q, ARG3Q, ARG723, ARG743, ARG823, ASN16Q, ASN503, ASP42Q, GLU43Q, GLY22Q, GLY483, GLY523, LEU4Q, LEU623, LEU753, LYS19Q, LYS44Q, LYS493, MET533, PHE12Q, PRO473, PRO833, TYR783, VAL513
• 1-2: ALA1071, ALA1131, ALA32, ALA411, ALA421, ALA461, ALA481, ALA772, ALA861, ALA961, ARG1011, ARG1121, ARG132, ARG361, ARG501, ARG531, ARG822, ARG832, ARG921, ASN441, ASN941, ASP911, ASP971, GLN1041, GLN112, GLN802, GLU1021, GLU1081, GLU1111, GLU152, GLU371, GLU432, GLU891, GLY412, GLY422, GLY431, GLY482, GLY871, GLY92, HIS872, ILE171, ILE42, ILE702, ILE881, LEU1091, LEU201, LEU382, LEU391, LEU392, LEU402, LEU712, LEU831, LEU951, LEU981, LYS102, LYS161, LYS442, LYS511, LYS541, LYS62, LYS742, LYS762, LYS841, LYS842, LYS851, LYS852, LYS931, PHE1061, PHE22, PHE401, PHE752, PRO1031, PRO502, SER732, THR452, THR492, THR72, TRP611, TYR122, TYR451, TYR471, TYR812, VAL1051, VAL142, VAL462, VAL472, VAL512, VAL52, VAL522, VAL722, VAL901
• 1-5: ALA145, ARG155, ARG281, GLY261, LEU271, LYS105, LYS135, LYS301, LYS341, LYS95, SER125, SER291, SER311, TRP251
• S-5: ALA245, ALA25, ALA32S, ALA385, ALA44S, ARG15S, ARG165, ARG18S, ARG195, ARG205, ARG33S, ARG37S, ARG42S, ARG88S, ARG90S, ARG92S, ASN34S, ASN40S, ASP175, ASP22S, ASP94S, CYS465, GLU30S, GLU31S, GLU485, GLU595, GLY43S, HIS225, HIS235, ILE24S, ILE35S, LEU19S, LEU23S, LEU255, LEU305, LEU36S, LYS16S, LYS27S, LYS35, LYS375, LYS405, LYS41S, MET395, PRO275, PRO285, PRO325, PRO415, PRO475, THR265, THR295, THR39S, THR445, TYR38S, VAL17S, VAL20S, VAL21S, VAL315, VAL455
• T-W: ALA10T, ALA39W, ALA42T, ALA46T, ALA4T, ALA91T, ARG26W, ARG30W, ARG36W, ASP6T, GLN38W, GLN43W, GLU27W, GLU31W, GLU34W, GLY42W, GLY94T, HIS31T, ILE41W, ILE57W, ILE89T, ILE8T, LEU13T, LEU32W, LEU44W, LEU61W, LEU92T, LEU9T, LYS28W, LYS29W, LYS48T, LYS54W, MET33W, PHE28T, PHE37W, PHE47T, PRO11T, SER40W, THR3T, THR45T, TRP29T, TYR18T, TYR5T, VAL12T, VAL25W, VAL30T, VAL43T, VAL7T
• T-7: ARG477, ARG497, ARG60T, ARG97, HIS58T, LYS487, LYS63T, TRP407, TYR69T
• 3-8: ARG553, LYS398
• 6-8: ALA226, ASN266, ASN338, CYS136, GLN358, GLU126, GLU246, ILE418, LEU106, LEU116, LEU328, LEU96, LYS256, LYS276, LYS368, THR236, TRP348