3F1H

crystal structure of a translation termination complex formed with release factor rf2. this file contains the 50s subunit of the second 70s ribosome. the entire crystal structure contains two 70s ribosomes as described in remark 400.

• Total interactions analyzed: 465
• Total true interactions: 29
• Strongest Interaction:
  • Chains: O-T
  • Int. Res.: 80
  • Norm. En. per Res.: -4.4688
• Hub Node: E(4)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
E-N	0.0	0.0	-33.6203	-33.6203	13	3	1	1330	0	0	2
E-O	0.0	1.9741	-24.7641	-22.79	16	1	0	952	0	8	8
E-R	-7.0786	3.0814	-30.7088	-34.706	16	11	0	1781	0	1	11
E-T	-4.5699	8.8756	-125.89	-121.5843	74	5	5	6641	3	20	18
F-P	0.0	13.2925	-89.902	-76.6095	40	8	1	4461	0	17	22
F-U	0.0	0.0	-1.5653	-1.5653	11	0	0	347	0	0	2
F-7	0.0	0.0	-0.0044	-0.0044	3	0	0	4	0	0	0
G-4	-35.4506	-28.5226	-129.637	-193.6102	66	6	3	6370	2	14	13
I-1	0.0	0.0	-43.1727	-43.1727	34	1	1	2073	0	8	9
K-Q	0.0	0.0	-0.0859	-0.0859	3	0	0	44	0	0	4
K-Z	-20.5207	3.2228	-22.8118	-40.1097	18	2	0	858	1	5	5
N-U	-16.4811	-0.2892	-103.216	-119.9864	46	6	1	4903	0	8	24
N-V	0.0	0.0	-0.0078	-0.0078	3	0	0	9	0	1	4
O-T	-58.5978	-66.6374	-232.272	-357.5072	80	22	5	9532	8	38	40
P-U	0.0	0.0	-0.0063	-0.0063	4	0	0	5	0	0	1
P-V	0.0	13.4139	-7.4695	5.9444	10	0	0	534	0	0	6
P-8	-10.9539	50.0658	-155.631	-116.5191	59	21	0	7951	0	17	57
Q-Z	-16.6574	-81.6106	-251.764	-350.032	116	18	6	12077	7	68	59
Q-0	0.0	0.0	-9.292	-9.292	10	0	0	462	0	0	5
R-W	0.0	6.814	-8.5101	-1.696	18	3	0	636	0	4	8
R-5	0.0	1.655	-59.375	-57.7199	33	3	4	2546	0	2	7
S-0	0.0	-3.4442	-9.5301	-12.9742	16	3	0	629	0	8	13
U-V	-18.6341	-1.6098	-220.085	-240.3289	91	37	11	10485	1	18	32
U-5	0.0	1.5763	-3.1336	-1.5574	12	0	0	484	0	0	10
W-5	0.0	46.2713	-87.3789	-41.1076	54	14	3	4644	0	10	21
X-2	-8.7895	-16.743	-99.0607	-124.5932	43	9	3	4378	2	10	10
X-7	0.0	0.0	-3.7679	-3.7679	9	4	0	321	0	1	6
0-8	0.0	0.0	-0.0225	-0.0225	2	0	0	18	0	2	1
6-8	0.0	0.0	-44.2735	-44.2735	20	7	0	1769	0	3	2

Interface residues

• E-N: ARG149E, GLY100N, GLY103N, GLY104N, GLY153E, LYS152E, LYS154E, PRO102N, SER99N, TYR101N, TYR151E, TYR98N, VAL150E
• E-O: ALA20E, ARG13E, ARG16E, ARG19E, ARG71O, ASP17E, ASP18E, ASP73O, GLU108O, GLY74O, LYS70O, PHE15E, PRO22E, PRO72O, SER75O, VAL21E
• E-R: ALA114E, ALA162E, ARG111E, ARG2R, GLY110E, GLY112E, GLY115E, GLY161E, HIS159E, HIS3R, LYS109E, LYS118E, LYS5R, PHE113E, PRO191E, TYR160E
• E-T: ALA20E, ALA28E, ALA5T, ARG13E, ARG13T, ARG16E, ARG16T, ARG19E, ARG3T, ARG53T, ASN180E, ASN192E, ASN55T, ASN58T, ASP174E, ASP17E, ASP18E, GLU11T, GLU178E, GLU179E, GLY10E, GLY193E, GLY29E, GLY4T, GLY6E, HIS79T, ILE14E, ILE176E, ILE26E, ILE75T, ILE7T, ILE83T, LEU181E, LEU182E, LEU183E, LEU27E, LEU52E, LEU6T, LEU78T, LEU82T, LEU9T, LYS33T, LYS35T, LYS8E, LYS8T, MET11E, MET1T, PHE15E, PHE51E, PHE57T, PHE61T, PHE76T, PRO177E, PRO53E, PRO77T, PRO81T, SER12T, SER80T, THR12E, THR24E, THR40T, THR59T, THR60T, TYR14T, TYR32T, VAL10T, VAL15T, VAL173E, VAL175E, VAL21E, VAL23E, VAL25E, VAL7E, VAL9E
• F-P: ALA113F, ALA12P, ARG117F, ARG119F, ARG15P, ARG16P, ARG188F, ARG18P, ARG38F, ARG44F, ARG7P, ARG95F, ASN13P, ASN29F, ASN9P, ASP116F, ASP5P, GLU120F, GLU190F, GLU35F, GLY11P, HIS31F, ILE186F, ILE28F, LEU32F, LEU33F, LEU41F, LEU6P, LYS14P, MET112F, PRO10P, PRO30F, PRO8P, THR189F, TRP34F, TYR184F, VAL183F, VAL187F, VAL36F, VAL37F
• F-U: ALA49F, ARG3U, ARG45F, ARG95F, GLY91F, PRO2U, PRO92F, THR51F, TYR97F, VAL84F, VAL88F
• F-7: ALA58F, LYS377, SER60F
• G-4: ALA110G, ALA384, ALA61G, ALA6G, ARG113G, ARG394, ARG98G, ASN108G, ASN464, ASP4G, ASP97G, CYS424, CYS624, CYS654, GLU104G, GLU143G, GLU494, GLU564, GLU604, GLY177G, GLY65G, ILE101G, ILE114G, ILE140G, ILE144G, ILE404, ILE414, ILE484, ILE574, ILE63G, LEU103G, LEU106G, LEU107G, LEU111G, LEU139G, LEU3G, LEU5G, LEU62G, LEU7G, LYS105G, LYS10G, LYS544, LYS644, LYS8G, MET148G, PHE102G, PHE141G, PRO112G, PRO142G, PRO179G, PRO2G, PRO374, PRO554, SER524, SER634, THR145G, THR504, THR534, THR64G, TRP100G, TYR514, TYR584, VAL109G, VAL364, VAL594, VAL614
• I-1: ALA26I, ALA39I, ALA641, ARG27I, ARG33I, ARG501, ARG521, ARG761, ASN28I, ASN43I, GLU41I, GLU721, GLU751, GLY24I, HIS661, ILE671, LEU31I, LEU38I, LEU731, LYS691, MET1I, PRO23I, PRO32I, PRO681, SER42I, SER651, THR40I, TYR29I, TYR711, VAL37I, VAL491, VAL511, VAL701, VAL741
• K-Q: ARG60Q, ARG93K, LYS90K
• K-Z: ALA109Z, ALA113Z, ARG112Z, ARG132K, ARG93K, GLU137K, GLU94K, GLY110Z, GLY114Z, GLY115Z, GLY135K, GLY92K, ILE146Z, LYS90K, LYS95K, PRO177Z, PRO91K, VAL111Z
• N-U: ALA67U, ALA68U, ALA96U, ALA99U, ARG101U, ARG35N, ARG58N, ARG59U, ARG62N, ARG64U, ARG70U, ASN66U, ASN68N, ASN94U, ASP56U, ASP64N, ASP97U, CYS69U, GLN71U, GLY59N, HIS61N, ILE65U, LEU60U, LEU95U, LEU98U, LYS25N, LYS30N, LYS52N, LYS60N, MET71N, PHE106U, PHE57U, PRO103U, PRO29N, PRO63N, PRO67N, THR26N, THR55N, THR66N, TRP61U, TRP65N, TYR27N, VAL100U, VAL28N, VAL63U, VAL69N
• N-V: ARG13V, LYS25N, TYR12V
• O-T: ALA103O, ALA118O, ALA11O, ALA76O, ALA83O, ARG103T, ARG104O, ARG107O, ARG39T, ARG41T, ARG51T, ARG64O, ARG64T, ARG71O, ARG74T, ARG78O, ARG97O, ASN104T, ASN38T, ASP44T, ASP73O, ASP80O, ASP81O, GLN43T, GLU105O, GLU108O, GLU120O, GLU36T, GLU46T, GLU68O, GLU73T, GLU9O, GLY100O, GLY37T, GLY69T, GLY71T, GLY74O, ILE50T, ILE75T, ILE77O, ILE83T, LEU122O, LEU82T, LYS33T, LYS35T, LYS65T, LYS66O, LYS70O, MET112O, PHE45T, PHE61T, PHE76T, PHE79O, PHE99O, PRO101O, PRO119O, PRO72O, PRO77T, PRO81T, SER106T, SER67T, SER75O, SER80T, THR60T, THR62T, THR65O, TYR100T, TYR32T, TYR68T, VAL102O, VAL115O, VAL121O, VAL30T, VAL34T, VAL63O, VAL63T, VAL66T, VAL69O, VAL70T, VAL72T
• P-U: ARG36U, GLY26P, LYS13U, SER25P
• P-V: ALA31P, ARG82V, ARG83V, GLN80V, GLY26P, GLY28P, HIS27P, THR30P, THR32P, TYR81V
• P-8: ALA108, ALA248, ALA558, ARG138, ARG308, ARG468, ARG49P, ARG50P, ARG55P, ARG578, ARG61P, ARG65P, ASP47P, GLN68P, GLU52P, GLU548, GLU568, GLY288, GLY458, GLY66P, GLY69P, GLY98, HIS78, ILE168, ILE588, LEU508, LEU59P, LEU608, LEU618, LEU628, LEU62P, LYS118, LYS128, LYS158, LYS268, LYS298, LYS448, LYS478, LYS58, LYS598, LYS64P, LYS88, MET258, MET48, MET60P, PHE488, PHE51P, PRO48P, PRO538, PRO63P, SER56P, THR178, THR278, THR57P, THR58P, THR68, VAL148, VAL228, VAL238
• Q-Z: ALA107Q, ALA136Q, ALA173Z, ALA184Z, ALA187Z, ALA20Q, ALA28Q, ALA36Q, ALA51Z, ALA53Q, ARG101Q, ARG122Z, ARG133Q, ARG134Q, ARG49Z, ARG51Q, ARG56Q, ARG59Q, ARG60Q, ARG67Q, ARG72Z, ARG79Z, ARG80Z, ARG81Z, ARG82Z, ASN75Z, ASP123Z, ASP135Q, ASP138Q, ASP179Z, ASP25Q, ASP31Q, ASP45Z, ASP77Z, GLN118Z, GLN50Z, GLN73Z, GLU105Q, GLU119Z, GLU139Q, GLU178Z, GLU181Z, GLU186Z, GLU38Q, GLU48Q, GLU84Z, GLY100Q, GLY108Q, GLY114Z, GLY115Z, GLY19Q, GLY23Q, GLY24Q, GLY30Q, GLY33Q, GLY61Q, GLY62Q, HIS54Z, HIS57Q, HIS85Z, ILE120Z, ILE131Q, ILE53Z, ILE64Q, ILE66Q, LEU117Z, LEU17Q, LEU183Z, LEU24Z, LEU37Q, LEU41Z, LEU76Z, LYS128Q, LYS130Q, LYS182Z, LYS18Q, LYS22Q, LYS46Z, LYS63Q, LYS78Z, LYS98Q, MET54Q, PHE29Q, PHE32Q, PHE44Z, PHE48Z, PHE58Q, PHE65Q, PRO176Z, PRO177Z, PRO39Q, PRO83Z, PRO99Q, SER52Z, THR129Q, THR21Q, TYR137Q, TYR26Q, TYR99Z, VAL102Q, VAL106Q, VAL109Q, VAL111Z, VAL116Z, VAL132Q, VAL174Z, VAL175Z, VAL180Z, VAL27Q, VAL35Q, VAL47Z, VAL52Q, VAL55Q, VAL71Z, VAL74Z, VAL86Z
• Q-0: ARG10Q, ARG82Q, ASN120, GLY84Q, LYS110, LYS11Q, LYS77Q, LYS85Q, MET83Q, THR100
• R-W: ALA101R, ALA109R, ARG103R, ARG104R, ARG105R, ARG37W, ARG42W, ASN40W, ASP107R, GLU102R, GLY106R, GLY108R, LYS41W, PHE46W, PRO110R, THR39W, TYR38W, TYR45W
• R-5: ALA101R, ALA112R, ALA535, ARG103R, ARG90R, ARG96R, CYS465, CYS495, GLU102R, GLU115R, GLU118R, GLY505, HIS435, LEU100R, LEU111R, LEU113R, LEU305, LEU98R, LYS405, LYS99R, MET395, PRO110R, PRO285, PRO415, PRO425, PRO475, THR445, TYR515, TYR525, VAL117R, VAL38R, VAL455, VAL97R
• S-0: ARG20S, ARG720, ASN16S, GLU43S, GLY480, LEU750, LYS19S, LYS460, LYS490, MET530, PHE12S, PRO470, THR430, TYR780, VAL510, VAL790
• U-V: ALA107U, ALA113U, ALA3V, ALA41U, ALA42U, ALA46U, ALA48U, ALA77V, ALA86U, ALA96U, ARG101U, ARG112U, ARG13V, ARG36U, ARG50U, ARG53U, ARG82V, ARG83V, ARG92U, ASN44U, ASN94U, ASP91U, ASP97U, GLN104U, GLN11V, GLN80V, GLU102U, GLU108U, GLU111U, GLU15V, GLU37U, GLU43V, GLU89U, GLY41V, GLY42V, GLY43U, GLY48V, GLY87U, GLY9V, HIS87V, ILE17U, ILE4V, ILE70V, ILE88U, LEU109U, LEU20U, LEU38V, LEU39U, LEU39V, LEU40V, LEU71V, LEU83U, LEU95U, LEU98U, LYS10V, LYS16U, LYS44V, LYS51U, LYS6V, LYS74V, LYS76V, LYS84U, LYS84V, LYS85U, LYS85V, LYS93U, PHE106U, PHE2V, PHE40U, PHE75V, PRO103U, PRO50V, SER73V, THR45V, THR49V, THR7V, TRP61U, TYR12V, TYR45U, TYR47U, TYR81V, VAL105U, VAL110U, VAL14V, VAL46V, VAL47V, VAL51V, VAL52V, VAL5V, VAL72V, VAL90U
• U-5: ALA145, ARG155, GLY26U, LEU27U, LYS105, LYS135, LYS30U, LYS34U, LYS95, SER125, THR115, TRP25U
• W-5: ALA245, ALA25, ALA32W, ALA385, ARG15W, ARG165, ARG18W, ARG195, ARG205, ARG33W, ARG37W, ARG88W, ARG90W, ARG92W, ASN34W, ASN40W, ASP22W, CYS365, CYS465, CYS495, GLU30W, GLU31W, GLU485, GLY43W, HIS235, ILE24W, ILE295, ILE35W, LEU19W, LEU23W, LEU255, LEU305, LEU36W, LYS16W, LYS27W, LYS35, LYS375, LYS405, LYS41W, MET395, PRO275, PRO285, PRO415, PRO425, PRO475, THR265, THR39W, THR445, TYR38W, VAL17W, VAL20W, VAL21W, VAL315, VAL455
• X-2: ALA10X, ALA392, ALA46X, ALA4X, ALA91X, ARG262, ARG302, ARG362, ASP6X, GLN382, GLU272, GLU312, GLU342, GLY422, GLY94X, ILE412, ILE572, ILE89X, ILE8X, LEU13X, LEU322, LEU612, LEU92X, LEU9X, LYS282, LYS292, LYS48X, MET332, PHE28X, PHE372, PHE47X, PRO11X, SER402, THR3X, THR45X, TRP29X, TYR18X, TYR26X, TYR5X, VAL12X, VAL252, VAL43X, VAL7X
• X-7: ARG417, ARG477, ARG60X, ARG97, ASP75X, LYS487, LYS63X, LYS72X, VAL467
• 0-8: ARG550, LYS398
• 6-8: ALA226, ASN266, ASN338, GLN358, GLU126, GLU246, GLU408, GLY388, ILE418, LEU106, LEU116, LEU328, LEU96, LYS256, LYS276, LYS368, SER378, THR236, TRP348, TYR396