2ZQB

crystal structure of a psychrotrophic rnasehi variant with sextuple thermostabilizing mutations

• Total interactions analyzed: 6
• Total true interactions: 5
• Strongest Interaction:
  • Chains: A-B
  • Int. Res.: 97
  • Norm. En. per Res.: -2.4372
• Hub Node: A(3)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
A-B	-73.1895	-3.0426	-160.173	-236.4051	97	16	2	7187	0	29	27
A-C	0.0	-10.7849	-48.3237	-59.1086	39	3	0	2341	0	9	8
A-D	0.0	0.82	-29.0695	-28.2495	33	1	0	1735	0	16	21
B-C	0.0	0.0	-2.4109	-2.4109	13	0	0	356	0	0	0
C-D	-67.5991	-1.8705	-150.154	-219.6236	95	13	2	7171	0	28	24

Interface residues

• A-B: ALA139A, ALA139B, ALA141A, ALA141B, ALA142A, ALA142B, ALA143A, ALA143B, ALA145A, ALA145B, ALA60A, ALA60B, ARG108A, ARG108B, ARG134A, ARG134B, ARG140A, ARG140B, ASN146A, ASN146B, ASP151B, GLN137A, GLN137B, GLN149A, GLN149B, GLU131A, GLU144A, GLU144B, GLU36A, GLU36B, GLU59A, GLU59B, GLU63A, GLU63B, GLY153B, GLY23A, GLY23B, GLY25A, GLY25B, GLY32A, GLY32B, GLY39A, GLY39B, GLY40A, GLY40B, HIS116A, HIS116B, HIS33A, HIS33B, ILE150A, ILE150B, ILE26A, ILE26B, LEU115A, LEU115B, LEU138A, LEU138B, LEU52A, LEU52B, LEU58A, LEU58B, LEU61A, LEU61B, LYS111B, LYS29A, LYS29B, LYS31A, LYS31B, LYS35A, LYS35B, LYS62A, LYS62B, MET28A, MET28B, MET37A, MET37B, PHE41A, PHE41B, PRO147A, PRO147B, PRO64A, SER38A, SER38B, THR148A, THR148B, THR152A, THR152B, THR34A, THR34B, TYR24A, TYR24B, TYR30A, TYR30B, VAL27A, VAL27B, VAL56A, VAL56B
• A-C: ALA130A, ALA141C, ALA143C, ALA145C, ARG121A, ARG140C, ASN146C, ASN18C, CYS15C, GLU131A, GLU144C, GLU3A, GLY128A, GLY17C, GLY20C, GLY32A, HIS129A, HIS136C, HIS33A, HIS8A, ILE68A, ILE7A, LEU16C, LEU43C, LEU4A, LEU6A, LYS29A, LYS31A, LYS5A, LYS66A, PHE10A, PRO147C, PRO19C, PRO21C, SER42C, THR148C, THR34A, THR70A, TYR30A
• A-D: ALA127D, ALA130D, ALA141D, ARG121A, ARG134D, ARG77A, ASN132D, ASP119A, CYS135D, GLN137D, GLN74A, GLU131D, GLU133D, GLY125D, GLY128A, GLY128D, HIS126D, HIS129A, HIS129D, HIS136D, HIS8A, ILE68A, LEU138D, LEU6A, LYS124A, LYS124D, LYS66A, MET81A, SER71A, THR70A, TRP120A, TRP122A, VAL123A
• B-C: ASN18C, GLN149B, GLY17C, GLY20C, ILE150B, LEU43C, PRO19C, PRO21C, SER42B, SER42C, THR148B, THR152B, TYR154C
• C-D: ALA130C, ALA139C, ALA139D, ALA141C, ALA141D, ALA142C, ALA142D, ALA143C, ALA143D, ALA145C, ALA145D, ALA57D, ALA60C, ALA60D, ARG108C, ARG108D, ARG134C, ARG134D, ARG140C, ARG140D, ASN146C, ASN146D, ASP151C, GLN137C, GLN137D, GLN149C, GLN149D, GLU144C, GLU144D, GLU36C, GLU36D, GLU59C, GLU59D, GLU63C, GLU63D, GLY23C, GLY23D, GLY25C, GLY25D, GLY32C, GLY32D, GLY39C, GLY39D, GLY40C, GLY40D, HIS116C, HIS116D, HIS33C, HIS33D, ILE150C, ILE150D, ILE26C, ILE26D, LEU115C, LEU115D, LEU138C, LEU138D, LEU52C, LEU52D, LEU58C, LEU58D, LEU61C, LEU61D, LYS29C, LYS29D, LYS31C, LYS31D, LYS35C, LYS35D, LYS62C, LYS62D, MET28C, MET28D, MET37C, MET37D, PHE41C, PHE41D, PRO147C, PRO147D, SER38C, SER38D, THR148C, THR148D, THR152C, THR152D, THR34C, THR34D, TYR24C, TYR24D, TYR30C, TYR30D, VAL27C, VAL27D, VAL56C, VAL56D