2NXH

structural and mechanistic changes along an engineered path from metallo to non-metallo kdo8p synthase.

• Total interactions analyzed: 66
• Total true interactions: 24
• Strongest Interaction:
  • Chains: G-H
  • Int. Res.: 146
  • Norm. En. per Res.: -4.0191
• Hub Node: A(5)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
A-B	-87.1174	-37.3249	-438.202	-562.6443	153	12	8	16938	11	40	54
A-C	0.0	0.0	-43.1208	-43.1208	20	1	0	1316	0	0	0
A-D	-57.7761	-18.4876	-361.041	-437.3047	132	7	22	15531	8	28	26
A-G	0.0	15.8757	-60.3901	-44.5144	43	7	1	2425	0	18	23
A-H	0.0	-1.0226	-18.072	-19.0946	12	2	0	744	1	10	4
B-C	-67.7252	-24.4908	-369.495	-461.711	131	12	22	15701	10	29	28
B-D	0.0	0.0	-45.2347	-45.2347	20	2	0	1326	0	0	0
B-G	-8.9847	-23.968	-32.3708	-65.3235	39	0	0	1539	1	20	19
C-D	-105.8577	-26.6657	-419.55	-552.0733	151	27	7	16831	9	40	54
E-F	-80.3344	-26.5396	-410.795	-517.669	148	15	8	16286	8	31	45
E-G	0.0	0.0	-45.9385	-45.9385	20	1	0	1314	0	0	0
E-H	-59.5047	-21.8341	-356.601	-437.9398	130	11	20	15652	10	27	27
F-G	-46.6377	-7.9183	-354.253	-408.809	129	13	22	15432	7	28	27
F-H	0.0	0.0	-47.7434	-47.7434	20	1	0	1358	0	0	0
G-H	-118.3453	-43.8508	-424.588	-586.7842	146	20	8	16413	11	35	50
G-I	0.0	-1.2997	-14.4757	-15.7754	12	2	0	613	0	12	6
G-J	0.0	10.9996	-52.0287	-41.0291	42	1	1	2047	0	15	21
H-J	-7.4198	-23.7613	-28.4574	-59.6384	36	1	0	1228	1	18	21
I-J	-122.4144	-32.1148	-432.04	-586.5692	147	15	8	16589	9	33	51
I-K	0.0	0.0	-44.9192	-44.9192	20	1	0	1326	0	0	0
I-L	-59.1206	-24.7284	-375.397	-459.2461	132	4	21	15839	10	28	28
J-K	-63.2553	-24.133	-353.481	-440.8693	127	14	21	15682	10	30	28
J-L	0.0	0.0	-43.5224	-43.5224	19	1	0	1298	0	0	0
K-L	-113.8277	-27.8926	-429.368	-571.0883	152	22	7	16902	7	37	56

Interface residues

• A-B: ALA1047A, ALA1102A, ALA1113A, ALA1130A, ALA2047B, ALA2102B, ALA2113B, ALA2130B, ARG1049A, ARG1056A, ARG1106A, ARG1154A, ARG1168A, ARG2049B, ARG2056B, ARG2106B, ARG2154B, ARG2168B, ASN1048A, ASN1136A, ASN1161A, ASN1162A, ASN2048B, ASN2136B, ASN2161B, ASN2162B, ASP1045A, ASP1109A, ASP1133A, ASP1166A, ASP1195A, ASP2045B, ASP2109B, ASP2133B, ASP2166B, ASP2195B, CYS1105A, CYS2105B, GLN1087A, GLN1107A, GLN1127A, GLN1188A, GLN2087B, GLN2107B, GLN2127B, GLN2188B, GLU1084A, GLU1139A, GLU1153A, GLU2084B, GLU2139B, GLU2153B, GLY1126A, GLY1144A, GLY1155A, GLY1159A, GLY1191A, GLY1192A, GLY1194A, GLY1198A, GLY2126B, GLY2144B, GLY2155B, GLY2159B, GLY2191B, GLY2192B, GLY2194B, HIS1053A, HIS1083A, HIS2053B, HIS2083B, ILE1052A, ILE1082A, ILE1172A, ILE2052B, ILE2082B, ILE2172B, LEU1104A, LEU1110A, LEU1111A, LEU1112A, LEU1129A, LEU1141A, LEU1163A, LEU1189A, LEU1193A, LEU2104B, LEU2110B, LEU2111B, LEU2112B, LEU2129B, LEU2141B, LEU2163B, LEU2189B, LEU2193B, LYS1046A, LYS1125A, LYS1135A, LYS1140A, LYS1142A, LYS1196A, LYS2046B, LYS2125B, LYS2135B, LYS2140B, LYS2142B, LYS2196B, PHE1103A, PHE1128A, PHE1143A, PHE1158A, PHE1167A, PHE2103B, PHE2128B, PHE2143B, PHE2158B, PHE2167B, PRO1101A, PRO1131A, PRO1190A, PRO2101B, PRO2131B, PRO2190B, SER1050A, SER1051A, SER1054A, SER1085A, SER1169A, SER1197A, SER2050B, SER2051B, SER2054B, SER2085B, SER2169B, SER2197B, THR1108A, THR1156A, THR1157A, THR2108B, THR2156B, THR2157B, TRP1086A, TRP1132A, TRP2086B, TRP2132B, TYR1160A, TYR2160B, VAL1137A, VAL1164A, VAL1165A, VAL2137B, VAL2164B, VAL2165B
• A-C: ASN1161A, ASN1162A, ASN3161C, ASN3162C, GLY1159A, GLY3159C, LEU1163A, LEU1189A, LEU3163C, LEU3189C, MET1200A, MET3200C, PHE1158A, PHE3158C, THR1157A, THR3157C, TYR1160A, TYR3160C, VAL1164A, VAL3164C
• A-D: ALA1130A, ALA1210A, ALA1211A, ALA1213A, ALA1249A, ALA1257A, ALA4130D, ALA4210D, ALA4211D, ALA4213D, ALA4249D, ALA4257D, ARG1168A, ARG1209A, ARG1254A, ARG4168D, ARG4201D, ARG4209D, ARG4254D, ASN1161A, ASN1162A, ASN4161D, ASN4162D, ASP1166A, ASP4166D, GLN1127A, GLN1175A, GLN1188A, GLN4127D, GLN4175D, GLN4188D, GLU1202A, GLU1244A, GLU1248A, GLU1252A, GLU1255A, GLU1262A, GLU4202D, GLU4248D, GLU4252D, GLU4255D, GLU4262D, GLY1159A, GLY1199A, GLY1215A, GLY1245A, GLY4155D, GLY4159D, GLY4199D, GLY4215D, GLY4245D, HIS1185A, ILE1172A, ILE1204A, ILE1208A, ILE1246A, ILE1247A, ILE1250A, ILE1253A, ILE4172D, ILE4204D, ILE4208D, ILE4246D, ILE4247D, ILE4250D, ILE4253D, LEU1129A, LEU1163A, LEU1170A, LEU1189A, LEU1207A, LEU1251A, LEU4129D, LEU4163D, LEU4170D, LEU4189D, LEU4207D, LEU4251D, LYS1125A, LYS1259A, LYS4259D, MET1173A, MET1200A, MET4173D, MET4200D, PHE1128A, PHE1158A, PHE1167A, PHE1203A, PHE1205A, PHE4128D, PHE4158D, PHE4167D, PHE4203D, PHE4205D, PRO1131A, PRO1171A, PRO1190A, PRO1206A, PRO4131D, PRO4171D, PRO4190D, PRO4206D, SER1169A, SER1186A, SER1258A, SER4169D, SER4186D, SER4258D, THR1156A, THR1157A, THR4156D, THR4157D, TRP1132A, TRP4132D, TYR1181A, TYR1260A, TYR1261A, TYR4260D, TYR4261D, VAL1164A, VAL1165A, VAL1187A, VAL1212A, VAL1214A, VAL1256A, VAL4164D, VAL4165D, VAL4187D, VAL4212D, VAL4214D, VAL4256D
• A-G: ALA1047A, ALA1066A, ALA3146G, ARG1049A, ARG1056A, ARG3119G, ASN3136G, ASP1045A, GLU1014A, GLU1016A, GLU1017A, GLU1061A, GLU3139G, GLY1057A, GLY1059A, GLY1063A, GLY3118G, GLY3144G, GLY3145G, HIS1053A, HIS1058A, ILE1013A, ILE1052A, LEU1060A, LEU3112G, LEU3141G, LYS1046A, LYS1065A, LYS3116G, LYS3135G, LYS3140G, LYS3142G, LYS3147G, PHE1055A, PHE3143G, SER1015A, SER1051A, SER1054A, THR3117G, TRP1086A, TRP3176G, TYR1062A, VAL3138G
• A-H: ALA1066A, GLU1016A, GLU1017A, GLU1061A, GLY1063A, HIS1058A, HIS4053H, ILE4052H, LYS1065A, LYS1069A, SER4051H, TYR1062A
• B-C: ALA2130B, ALA2210B, ALA2211B, ALA2213B, ALA2249B, ALA2257B, ALA3130C, ALA3210C, ALA3211C, ALA3213C, ALA3249C, ALA3257C, ARG2168B, ARG2201B, ARG2209B, ARG2254B, ARG3168C, ARG3209C, ARG3254C, ASN2161B, ASN2162B, ASN3161C, ASN3162C, ASP2166B, ASP3166C, GLN2127B, GLN2175B, GLN2188B, GLN3127C, GLN3175C, GLN3188C, GLU2202B, GLU2244B, GLU2248B, GLU2252B, GLU2255B, GLU2262B, GLU3202C, GLU3248C, GLU3252C, GLU3255C, GLU3262C, GLY2159B, GLY2199B, GLY2215B, GLY2245B, GLY3155C, GLY3159C, GLY3199C, GLY3215C, GLY3245C, ILE2172B, ILE2204B, ILE2208B, ILE2246B, ILE2250B, ILE2253B, ILE3172C, ILE3204C, ILE3208C, ILE3246C, ILE3247C, ILE3250C, ILE3253C, LEU2129B, LEU2163B, LEU2170B, LEU2189B, LEU2207B, LEU2251B, LEU3129C, LEU3163C, LEU3170C, LEU3189C, LEU3207C, LEU3251C, LYS2125B, LYS2259B, LYS3125C, LYS3259C, MET2173B, MET2200B, MET3173C, MET3200C, PHE2128B, PHE2158B, PHE2167B, PHE2203B, PHE2205B, PHE3128C, PHE3158C, PHE3167C, PHE3203C, PHE3205C, PRO2131B, PRO2171B, PRO2190B, PRO2206B, PRO3131C, PRO3171C, PRO3190C, PRO3206C, SER2169B, SER2186B, SER2258B, SER3169C, SER3186C, SER3258C, THR2156B, THR2157B, THR3156C, THR3157C, TRP2132B, TRP3132C, TYR2181B, TYR2260B, TYR2261B, TYR3260C, TYR3261C, VAL2164B, VAL2165B, VAL2187B, VAL2212B, VAL2214B, VAL2256B, VAL3164C, VAL3165C, VAL3187C, VAL3212C, VAL3214C, VAL3256C
• B-D: ASN2161B, ASN2162B, ASN4161D, ASN4162D, GLY2159B, GLY4159D, LEU2163B, LEU2189B, LEU4163D, LEU4189D, MET2200B, MET4200D, PHE2158B, PHE4158D, THR2157B, THR4157D, TYR2160B, TYR4160D, VAL2164B, VAL4164D
• B-G: ALA2092B, ALA2115B, ALA3092G, ALA3115G, ALA3120G, ARG3119G, ASP2109B, ASP3096G, GLU2089B, GLU2093B, GLU3035G, GLU3037G, GLU3072G, GLU3093G, GLU3148G, GLY2118B, GLY2144B, GLY2145B, GLY3075G, GLY3118G, ILE3097G, LEU2112B, LYS2116B, LYS2140B, LYS2142B, LYS2147B, LYS3003G, LYS3034G, LYS3071G, LYS3077G, LYS3116G, LYS3147G, LYS3178G, PHE2143B, PHE3004G, THR2108B, THR2117B, THR3117G, TYR3150G
• C-D: ALA3047C, ALA3102C, ALA3113C, ALA3130C, ALA4047D, ALA4102D, ALA4113D, ALA4130D, ARG3049C, ARG3056C, ARG3106C, ARG3154C, ARG3168C, ARG4049D, ARG4056D, ARG4106D, ARG4154D, ARG4168D, ASN3048C, ASN3136C, ASN3161C, ASN3162C, ASN4048D, ASN4136D, ASN4161D, ASN4162D, ASP3045C, ASP3109C, ASP3133C, ASP3166C, ASP3195C, ASP4045D, ASP4109D, ASP4133D, ASP4166D, ASP4195D, CYS3105C, CYS4105D, GLN3087C, GLN3107C, GLN3127C, GLN3188C, GLN4087D, GLN4107D, GLN4127D, GLN4188D, GLU3084C, GLU3139C, GLU3153C, GLU4084D, GLU4139D, GLU4153D, GLY3126C, GLY3144C, GLY3155C, GLY3159C, GLY3191C, GLY3192C, GLY3194C, GLY4126D, GLY4144D, GLY4155D, GLY4159D, GLY4191D, GLY4192D, GLY4194D, HIS3053C, HIS3083C, HIS4053D, HIS4083D, ILE3052C, ILE3082C, ILE3172C, ILE4052D, ILE4082D, ILE4172D, LEU3104C, LEU3110C, LEU3111C, LEU3112C, LEU3129C, LEU3141C, LEU3163C, LEU3193C, LEU4104D, LEU4110D, LEU4111D, LEU4112D, LEU4129D, LEU4141D, LEU4163D, LEU4189D, LEU4193D, LYS3046C, LYS3125C, LYS3135C, LYS3140C, LYS3142C, LYS3196C, LYS4046D, LYS4125D, LYS4135D, LYS4140D, LYS4142D, LYS4196D, PHE3103C, PHE3128C, PHE3143C, PHE3158C, PHE3167C, PHE4103D, PHE4128D, PHE4143D, PHE4158D, PHE4167D, PRO3101C, PRO3131C, PRO3190C, PRO4101D, PRO4131D, PRO4190D, SER3050C, SER3051C, SER3054C, SER3085C, SER3169C, SER3197C, SER4050D, SER4051D, SER4054D, SER4085D, SER4169D, SER4197D, THR3108C, THR3156C, THR3157C, THR4108D, THR4156D, THR4157D, TRP3086C, TRP3132C, TRP4086D, TRP4132D, TYR3160C, TYR4160D, VAL3137C, VAL3164C, VAL3165C, VAL4137D, VAL4164D, VAL4165D
• E-F: ALA1047E, ALA1102E, ALA1113E, ALA1130E, ALA2047F, ALA2102F, ALA2113F, ALA2130F, ARG1049E, ARG1056E, ARG1106E, ARG1154E, ARG1168E, ARG2049F, ARG2056F, ARG2106F, ARG2154F, ARG2168F, ASN1048E, ASN1136E, ASN1161E, ASN1162E, ASN2048F, ASN2136F, ASN2161F, ASN2162F, ASP1045E, ASP1109E, ASP1133E, ASP1166E, ASP2045F, ASP2109F, ASP2133F, ASP2166F, ASP2195F, CYS1105E, CYS2105F, GLN1087E, GLN1107E, GLN1127E, GLN1188E, GLN2087F, GLN2107F, GLN2127F, GLN2188F, GLU1084E, GLU1139E, GLU1153E, GLU2084F, GLU2139F, GLU2153F, GLY1126E, GLY1144E, GLY1155E, GLY1159E, GLY1191E, GLY1192E, GLY2126F, GLY2144F, GLY2155F, GLY2159F, GLY2191F, GLY2192F, GLY2194F, HIS1053E, HIS1083E, HIS2053F, HIS2083F, ILE1052E, ILE1082E, ILE1172E, ILE2052F, ILE2082F, ILE2172F, LEU1104E, LEU1110E, LEU1111E, LEU1112E, LEU1129E, LEU1141E, LEU1163E, LEU2104F, LEU2110F, LEU2111F, LEU2112F, LEU2129F, LEU2141F, LEU2163F, LEU2189F, LEU2193F, LYS1046E, LYS1125E, LYS1135E, LYS1140E, LYS1142E, LYS2046F, LYS2125F, LYS2135F, LYS2140F, LYS2142F, LYS2196F, PHE1103E, PHE1128E, PHE1143E, PHE1158E, PHE1167E, PHE2103F, PHE2128F, PHE2143F, PHE2158F, PHE2167F, PRO1101E, PRO1131E, PRO1190E, PRO2101F, PRO2131F, PRO2190F, SER1050E, SER1051E, SER1054E, SER1085E, SER1169E, SER1197E, SER2050F, SER2051F, SER2054F, SER2085F, SER2169F, SER2197F, THR1108E, THR1156E, THR1157E, THR2108F, THR2156F, THR2157F, TRP1086E, TRP1132E, TRP2086F, TRP2132F, TYR1160E, TYR2160F, VAL1137E, VAL1138E, VAL1164E, VAL1165E, VAL2137F, VAL2164F, VAL2165F
• E-G: ASN1161E, ASN1162E, ASN3161G, ASN3162G, GLY1159E, GLY3159G, LEU1163E, LEU1189E, LEU3163G, LEU3189G, MET1200E, MET3200G, PHE1158E, PHE3158G, THR1157E, THR3157G, TYR1160E, TYR3160G, VAL1164E, VAL3164G
• E-H: ALA1130E, ALA1210E, ALA1211E, ALA1213E, ALA1249E, ALA1257E, ALA4130H, ALA4210H, ALA4211H, ALA4213H, ALA4249H, ALA4257H, ARG1168E, ARG1209E, ARG1254E, ARG4168H, ARG4201H, ARG4209H, ARG4254H, ASN1161E, ASN1162E, ASN4161H, ASN4162H, ASP1166E, ASP4166H, GLN1127E, GLN1175E, GLN1188E, GLN4127H, GLN4175H, GLN4188H, GLU1202E, GLU1244E, GLU1248E, GLU1252E, GLU1255E, GLU1262E, GLU4202H, GLU4248H, GLU4252H, GLU4255H, GLU4262H, GLY1159E, GLY1199E, GLY1215E, GLY1245E, GLY4159H, GLY4199H, GLY4215H, GLY4245H, ILE1172E, ILE1204E, ILE1208E, ILE1246E, ILE1250E, ILE1253E, ILE4172H, ILE4204H, ILE4208H, ILE4246H, ILE4247H, ILE4250H, ILE4253H, LEU1129E, LEU1163E, LEU1170E, LEU1189E, LEU1207E, LEU1251E, LEU4129H, LEU4163H, LEU4170H, LEU4189H, LEU4207H, LEU4251H, LYS1125E, LYS1259E, LYS4125H, LYS4259H, MET1173E, MET1200E, MET4173H, MET4200H, PHE1128E, PHE1158E, PHE1167E, PHE1203E, PHE1205E, PHE4128H, PHE4158H, PHE4167H, PHE4203H, PHE4205H, PRO1131E, PRO1171E, PRO1190E, PRO1206E, PRO4131H, PRO4171H, PRO4190H, PRO4206H, SER1169E, SER1186E, SER1258E, SER4169H, SER4186H, SER4258H, THR1156E, THR1157E, THR4156H, THR4157H, TRP1132E, TRP4132H, TYR1181E, TYR1260E, TYR1261E, TYR4260H, TYR4261H, VAL1164E, VAL1165E, VAL1187E, VAL1212E, VAL1214E, VAL1256E, VAL4164H, VAL4165H, VAL4187H, VAL4212H, VAL4214H, VAL4256H
• F-G: ALA2130F, ALA2210F, ALA2211F, ALA2213F, ALA2249F, ALA2257F, ALA3130G, ALA3210G, ALA3211G, ALA3213G, ALA3249G, ALA3257G, ARG2168F, ARG2209F, ARG2254F, ARG3168G, ARG3209G, ARG3254G, ASN2161F, ASN2162F, ASN3161G, ASN3162G, ASP2166F, ASP3166G, GLN2127F, GLN2175F, GLN2188F, GLN3127G, GLN3175G, GLN3188G, GLU2202F, GLU2248F, GLU2252F, GLU2255F, GLU2262F, GLU3202G, GLU3248G, GLU3252G, GLU3255G, GLU3262G, GLY2159F, GLY2199F, GLY2215F, GLY2245F, GLY3155G, GLY3159G, GLY3199G, GLY3215G, GLY3245G, ILE2172F, ILE2204F, ILE2208F, ILE2246F, ILE2247F, ILE2250F, ILE2253F, ILE3172G, ILE3204G, ILE3208G, ILE3246G, ILE3250G, ILE3253G, LEU2129F, LEU2163F, LEU2170F, LEU2189F, LEU2207F, LEU2251F, LEU3129G, LEU3163G, LEU3170G, LEU3189G, LEU3207G, LEU3251G, LYS2125F, LYS2259F, LYS3125G, LYS3259G, MET2173F, MET2200F, MET3173G, MET3200G, PHE2128F, PHE2158F, PHE2167F, PHE2203F, PHE2205F, PHE3128G, PHE3158G, PHE3167G, PHE3203G, PHE3205G, PRO2131F, PRO2171F, PRO2190F, PRO2206F, PRO3131G, PRO3171G, PRO3190G, PRO3206G, SER2169F, SER2186F, SER2258F, SER3169G, SER3186G, SER3258G, THR2156F, THR2157F, THR3156G, THR3157G, TRP2132F, TRP3132G, TYR2181F, TYR2260F, TYR2261F, TYR3260G, TYR3261G, VAL2164F, VAL2165F, VAL2187F, VAL2212F, VAL2214F, VAL2256F, VAL3164G, VAL3165G, VAL3187G, VAL3212G, VAL3214G, VAL3256G
• F-H: ASN2161F, ASN2162F, ASN4161H, ASN4162H, GLY2159F, GLY4159H, LEU2163F, LEU2189F, LEU4163H, LEU4189H, MET2200F, MET4200H, PHE2158F, PHE4158H, THR2157F, THR4157H, TYR2160F, TYR4160H, VAL2164F, VAL4164H
• G-H: ALA3047G, ALA3102G, ALA3113G, ALA3130G, ALA4047H, ALA4102H, ALA4113H, ALA4130H, ARG3049G, ARG3056G, ARG3106G, ARG3154G, ARG3168G, ARG4049H, ARG4056H, ARG4106H, ARG4154H, ARG4168H, ASN3048G, ASN3136G, ASN3161G, ASN3162G, ASN4048H, ASN4136H, ASN4161H, ASN4162H, ASP3045G, ASP3109G, ASP3133G, ASP3166G, ASP3195G, ASP4045H, ASP4109H, ASP4133H, ASP4166H, CYS3105G, CYS4105H, GLN3087G, GLN3107G, GLN3127G, GLN3188G, GLN4087H, GLN4107H, GLN4127H, GLN4188H, GLU3084G, GLU3139G, GLU3153G, GLU4084H, GLU4139H, GLU4153H, GLY3126G, GLY3144G, GLY3155G, GLY3159G, GLY3191G, GLY3192G, GLY3194G, GLY4126H, GLY4144H, GLY4155H, GLY4159H, GLY4191H, HIS3053G, HIS3083G, HIS4053H, HIS4083H, ILE3052G, ILE3082G, ILE3172G, ILE4052H, ILE4082H, ILE4172H, LEU3104G, LEU3110G, LEU3111G, LEU3112G, LEU3129G, LEU3141G, LEU3163G, LEU3189G, LEU3193G, LEU4104H, LEU4110H, LEU4111H, LEU4112H, LEU4129H, LEU4141H, LEU4163H, LEU4189H, LYS3046G, LYS3125G, LYS3135G, LYS3140G, LYS3142G, LYS3196G, LYS4046H, LYS4125H, LYS4135H, LYS4140H, LYS4142H, PHE3103G, PHE3128G, PHE3143G, PHE3158G, PHE3167G, PHE4103H, PHE4128H, PHE4143H, PHE4158H, PHE4167H, PRO3101G, PRO3131G, PRO3190G, PRO4101H, PRO4131H, PRO4190H, SER3050G, SER3051G, SER3054G, SER3085G, SER3169G, SER3197G, SER4050H, SER4051H, SER4054H, SER4085H, SER4169H, THR3108G, THR3156G, THR3157G, THR4108H, THR4156H, THR4157H, TRP3086G, TRP3132G, TRP4086H, TRP4132H, TYR3160G, TYR4160H, VAL3137G, VAL3164G, VAL3165G, VAL4137H, VAL4164H, VAL4165H
• G-I: ALA3066G, ASP1195I, GLU3016G, GLU3061G, GLY3063G, HIS1053I, HIS3058G, ILE1052I, LYS3065G, LYS3069G, SER1051I, TYR3062G
• G-J: ALA2146J, ALA3047G, ALA3066G, ARG2119J, ARG3056G, ASN2136J, ASP3045G, GLN3087G, GLU2139J, GLU3014G, GLU3016G, GLU3017G, GLU3061G, GLY2118J, GLY2144J, GLY2145J, GLY3057G, GLY3059G, GLY3063G, HIS3053G, HIS3058G, ILE3013G, ILE3052G, LEU2112J, LEU2141J, LEU3060G, LEU3231G, LYS2116J, LYS2135J, LYS2140J, LYS2142J, LYS2147J, LYS3065G, PHE2143J, PHE3044G, PHE3055G, SER3015G, THR2117J, TRP2176J, TRP3086G, TYR3062G, VAL2138J
• H-J: ALA2092J, ALA2095J, ALA2115J, ALA2120J, ALA4115H, ARG2119J, ASP2096J, ASP4109H, GLU2035J, GLU2037J, GLU2072J, GLU2093J, GLU2148J, GLU4089H, GLU4093H, GLY2075J, GLY2118J, GLY4118H, GLY4144H, GLY4145H, LEU4112H, LYS2003J, LYS2071J, LYS2077J, LYS2116J, LYS2147J, LYS2178J, LYS4116H, LYS4140H, LYS4142H, LYS4147H, PHE4143H, THR2117J, THR4108H, THR4117H, TYR2150J
• I-J: ALA1047I, ALA1102I, ALA1113I, ALA1130I, ALA2047J, ALA2102J, ALA2113J, ALA2130J, ARG1049I, ARG1056I, ARG1106I, ARG1154I, ARG1168I, ARG2049J, ARG2056J, ARG2106J, ARG2154J, ARG2168J, ASN1048I, ASN1136I, ASN1161I, ASN1162I, ASN2048J, ASN2136J, ASN2161J, ASN2162J, ASP1045I, ASP1109I, ASP1133I, ASP1166I, ASP1195I, ASP2045J, ASP2109J, ASP2133J, ASP2166J, CYS1105I, CYS2105J, GLN1087I, GLN1107I, GLN1127I, GLN1188I, GLN2087J, GLN2107J, GLN2127J, GLN2188J, GLU1084I, GLU1139I, GLU1153I, GLU2084J, GLU2139J, GLU2153J, GLY1126I, GLY1144I, GLY1155I, GLY1159I, GLY1191I, GLY1192I, GLY1194I, GLY2126J, GLY2144J, GLY2155J, GLY2159J, GLY2191J, HIS1053I, HIS1083I, HIS2053J, HIS2083J, ILE1052I, ILE1082I, ILE1172I, ILE2052J, ILE2082J, ILE2172J, LEU1104I, LEU1110I, LEU1111I, LEU1112I, LEU1129I, LEU1141I, LEU1163I, LEU1189I, LEU1193I, LEU2104J, LEU2110J, LEU2111J, LEU2112J, LEU2129J, LEU2141J, LEU2163J, LEU2189J, LYS1046I, LYS1125I, LYS1135I, LYS1140I, LYS1142I, LYS1196I, LYS2046J, LYS2125J, LYS2135J, LYS2140J, LYS2142J, PHE1044I, PHE1103I, PHE1128I, PHE1143I, PHE1158I, PHE1167I, PHE2103J, PHE2128J, PHE2143J, PHE2158J, PHE2167J, PRO1101I, PRO1131I, PRO1190I, PRO2101J, PRO2131J, PRO2190J, SER1050I, SER1051I, SER1054I, SER1085I, SER1169I, SER1197I, SER2050J, SER2051J, SER2054J, SER2085J, SER2169J, THR1108I, THR1156I, THR1157I, THR2108J, THR2156J, THR2157J, TRP1086I, TRP1132I, TRP2086J, TRP2132J, TYR1160I, TYR2160J, VAL1137I, VAL1164I, VAL1165I, VAL2137J, VAL2164J, VAL2165J
• I-K: ASN1161I, ASN1162I, ASN3161K, ASN3162K, GLY1159I, GLY3159K, LEU1163I, LEU1189I, LEU3163K, LEU3189K, MET1200I, MET3200K, PHE1158I, PHE3158K, THR1157I, THR3157K, TYR1160I, TYR3160K, VAL1164I, VAL3164K
• I-L: ALA1130I, ALA1210I, ALA1211I, ALA1213I, ALA1249I, ALA1257I, ALA4130L, ALA4210L, ALA4211L, ALA4213L, ALA4249L, ALA4257L, ARG1168I, ARG1209I, ARG1254I, ARG4168L, ARG4201L, ARG4209L, ARG4254L, ASN1161I, ASN1162I, ASN4161L, ASN4162L, ASP1166I, ASP4166L, GLN1127I, GLN1175I, GLN1188I, GLN4127L, GLN4175L, GLN4188L, GLU1202I, GLU1248I, GLU1252I, GLU1255I, GLU1262I, GLU4202L, GLU4248L, GLU4252L, GLU4255L, GLU4262L, GLY1155I, GLY1159I, GLY1199I, GLY1215I, GLY1245I, GLY4155L, GLY4159L, GLY4199L, GLY4215L, GLY4245L, ILE1172I, ILE1204I, ILE1208I, ILE1246I, ILE1250I, ILE1253I, ILE4172L, ILE4204L, ILE4208L, ILE4246L, ILE4247L, ILE4250L, ILE4253L, LEU1129I, LEU1163I, LEU1170I, LEU1189I, LEU1207I, LEU1251I, LEU4129L, LEU4163L, LEU4170L, LEU4189L, LEU4207L, LEU4251L, LYS1125I, LYS1259I, LYS4125L, LYS4259L, MET1173I, MET1200I, MET4173L, MET4200L, PHE1128I, PHE1158I, PHE1167I, PHE1203I, PHE1205I, PHE4128L, PHE4158L, PHE4167L, PHE4203L, PHE4205L, PRO1131I, PRO1171I, PRO1190I, PRO1206I, PRO4131L, PRO4171L, PRO4190L, PRO4206L, SER1169I, SER1186I, SER1258I, SER4169L, SER4186L, SER4258L, THR1156I, THR1157I, THR1263I, THR4156L, THR4157L, TRP1132I, TRP4132L, TYR1181I, TYR1260I, TYR1261I, TYR4260L, TYR4261L, VAL1164I, VAL1165I, VAL1187I, VAL1212I, VAL1214I, VAL1256I, VAL4164L, VAL4165L, VAL4187L, VAL4212L, VAL4214L, VAL4256L
• J-K: ALA2130J, ALA2210J, ALA2211J, ALA2213J, ALA2249J, ALA2257J, ALA3130K, ALA3210K, ALA3211K, ALA3213K, ALA3249K, ALA3257K, ARG2168J, ARG2209J, ARG2254J, ARG3168K, ARG3209K, ARG3254K, ASN2161J, ASN2162J, ASN3161K, ASN3162K, ASP2166J, ASP3166K, GLN2127J, GLN2175J, GLN2188J, GLN3127K, GLN3175K, GLN3188K, GLU2202J, GLU2248J, GLU2252J, GLU2255J, GLU2262J, GLU3202K, GLU3248K, GLU3252K, GLU3255K, GLU3262K, GLY2159J, GLY2199J, GLY2215J, GLY2245J, GLY3159K, GLY3199K, GLY3215K, GLY3245K, HIS3185K, ILE2172J, ILE2204J, ILE2208J, ILE2246J, ILE2250J, ILE2253J, ILE3172K, ILE3204K, ILE3208K, ILE3246K, ILE3250K, ILE3253K, LEU2129J, LEU2163J, LEU2170J, LEU2189J, LEU2207J, LEU2251J, LEU3129K, LEU3163K, LEU3170K, LEU3189K, LEU3207K, LEU3251K, LYS2125J, LYS2259J, LYS3259K, MET2173J, MET2200J, MET3173K, MET3200K, PHE2128J, PHE2158J, PHE2167J, PHE2203J, PHE2205J, PHE3128K, PHE3158K, PHE3167K, PHE3203K, PHE3205K, PRO2131J, PRO2171J, PRO2190J, PRO2206J, PRO3131K, PRO3171K, PRO3190K, PRO3206K, SER2169J, SER2186J, SER2258J, SER3169K, SER3186K, SER3258K, THR2156J, THR2157J, THR3156K, THR3157K, TRP2132J, TRP3132K, TYR2181J, TYR2260J, TYR2261J, TYR3260K, TYR3261K, VAL2164J, VAL2165J, VAL2187J, VAL2212J, VAL2214J, VAL2256J, VAL3164K, VAL3165K, VAL3187K, VAL3212K, VAL3214K, VAL3256K
• J-L: ASN2161J, ASN2162J, ASN4161L, ASN4162L, GLY2159J, GLY4159L, LEU2163J, LEU4163L, LEU4189L, MET2200J, MET4200L, PHE2158J, PHE4158L, THR2157J, THR4157L, TYR2160J, TYR4160L, VAL2164J, VAL4164L
• K-L: ALA3047K, ALA3102K, ALA3113K, ALA3130K, ALA4047L, ALA4102L, ALA4113L, ALA4130L, ARG3049K, ARG3056K, ARG3106K, ARG3154K, ARG3168K, ARG4049L, ARG4056L, ARG4106L, ARG4154L, ARG4168L, ASN3048K, ASN3136K, ASN3161K, ASN3162K, ASN4048L, ASN4136L, ASN4161L, ASN4162L, ASP3045K, ASP3109K, ASP3133K, ASP3166K, ASP3195K, ASP4045L, ASP4109L, ASP4133L, ASP4166L, ASP4195L, CYS3105K, CYS4105L, GLN3087K, GLN3107K, GLN3127K, GLN3188K, GLN4087L, GLN4107L, GLN4127L, GLN4188L, GLU3084K, GLU3139K, GLU3153K, GLU4084L, GLU4139L, GLU4153L, GLY3126K, GLY3144K, GLY3155K, GLY3159K, GLY3191K, GLY3192K, GLY3194K, GLY4126L, GLY4144L, GLY4155L, GLY4159L, GLY4191L, GLY4192L, GLY4194L, HIS3053K, HIS3083K, HIS4053L, HIS4083L, ILE3052K, ILE3082K, ILE3172K, ILE4052L, ILE4082L, ILE4172L, LEU3104K, LEU3110K, LEU3111K, LEU3112K, LEU3129K, LEU3141K, LEU3163K, LEU3189K, LEU4104L, LEU4110L, LEU4111L, LEU4112L, LEU4129L, LEU4141L, LEU4163L, LEU4193L, LYS3046K, LYS3125K, LYS3135K, LYS3140K, LYS3142K, LYS3196K, LYS4046L, LYS4125L, LYS4135L, LYS4140L, LYS4142L, LYS4196L, PHE3103K, PHE3128K, PHE3143K, PHE3158K, PHE3167K, PHE4103L, PHE4128L, PHE4143L, PHE4158L, PHE4167L, PRO3101K, PRO3131K, PRO3190K, PRO4101L, PRO4131L, PRO4190L, SER3050K, SER3051K, SER3054K, SER3085K, SER3169K, SER3197K, SER4050L, SER4051L, SER4054L, SER4085L, SER4169L, SER4197L, THR3108K, THR3156K, THR3157K, THR4108L, THR4156L, THR4157L, TRP3086K, TRP3132K, TRP4086L, TRP4132L, TYR3160K, TYR4160L, VAL3137K, VAL3138K, VAL3164K, VAL3165K, VAL4137L, VAL4138L, VAL4164L, VAL4165L