2JK0

structural and functional insights into erwinia carotovora l-asparaginase

• Total interactions analyzed: 28
• Total true interactions: 15
• Strongest Interaction:
  • Chains: F-G
  • Int. Res.: 94
  • Norm. En. per Res.: -6.0904
• Hub Node: B(4)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
A-B	-48.4062	-119.5657	-198.255	-366.2269	91	17	5	9179	10	16	13
A-C	-100.2927	-74.6822	-650.457	-825.432	215	54	18	24497	12	56	60
A-D	-87.9834	-186.8837	-284.71	-559.5771	97	14	6	10883	14	37	35
B-C	-96.1562	-182.5094	-280.88	-559.5456	95	24	6	10896	8	38	34
B-D	-106.2508	-77.016	-696.018	-879.2848	204	47	19	24164	12	55	60
B-F	-5.3469	12.5038	-69.1546	-61.9978	53	0	8	3577	0	24	23
B-G	-2.5051	3.2469	-33.6722	-32.9304	30	0	0	1422	3	15	16
C-D	-56.9999	-116.5739	-213.737	-387.3109	91	18	5	8987	9	16	15
C-F	-13.4745	-38.2198	-26.637	-78.3312	28	2	0	1242	4	13	17
E-F	-22.8003	-108.4894	-185.488	-316.7777	87	9	4	8439	10	16	12
E-G	-96.8089	-76.6064	-682.744	-856.1593	206	49	19	24356	13	57	59
E-H	-117.8009	-164.387	-288.42	-570.6079	95	20	6	10882	9	37	33
F-G	-115.2991	-174.3221	-282.88	-572.5012	94	15	6	10950	10	38	33
F-H	-114.5457	-81.4874	-707.814	-903.8471	206	41	19	24491	11	55	61
G-H	-50.0277	-131.9951	-203.346	-385.3688	81	16	5	8211	12	18	13

Interface residues

• A-B: ALA120A, ALA126A, ALA126B, ALA129A, ALA129B, ALA171A, ALA171B, ALA22A, ALA22B, ALA42A, ALA42B, ARG122A, ARG122B, ARG159A, ARG159B, ASN134A, ASN134B, ASN157A, ASN157B, ASN170A, ASN170B, ASN23A, ASP130A, ASP130B, ASP158A, ASP158B, ASP175A, ASP175B, ASP191A, ASP191B, GLN25A, GLU45A, GLU45B, GLY131A, GLY131B, GLY137A, GLY137B, GLY190A, GLY190B, GLY28A, ILE127A, ILE127B, ILE188A, ILE188B, ILE189A, ILE189B, LEU135A, LEU135B, LEU156A, LEU156B, LEU174A, LEU174B, LEU46A, LEU46B, LEU49A, LEU49B, LYS140B, LYS178A, LYS178B, LYS192B, MET121A, MET121B, MET133A, MET133B, PRO123A, PRO132A, PRO132B, PRO44A, PRO44B, SER128A, SER128B, SER172A, SER172B, THR125B, THR169B, THR173A, THR173B, THR176A, THR176B, THR24A, THR26A, THR27A, THR48B, TYR136A, TYR136B, TYR184A, TYR184B, VAL187A, VAL187B, VAL43A, VAL43B
• A-C: ALA171A, ALA171C, ALA219A, ALA219C, ALA236A, ALA236C, ALA248A, ALA248C, ALA252A, ALA252C, ALA288C, ALA301A, ALA301C, ARG258A, ARG258C, ARG275C, ARG278A, ARG278C, ARG304A, ARG304C, ASN170A, ASN170C, ASN64A, ASN64C, ASP175A, ASP175C, ASP221A, ASP221C, ASP228A, ASP228C, ASP229A, ASP229C, ASP235A, ASP235C, ASP260A, ASP260C, ASP287C, ASP296A, ASP296C, ASP68A, ASP68C, ASP96A, ASP96C, ASP99A, ASP99C, GLN227A, GLN227C, GLN290A, GLN290C, GLU100A, GLU100C, GLU181A, GLU181C, GLU182A, GLU182C, GLU231A, GLU231C, GLU63A, GLU63C, GLY225A, GLY225C, GLY241A, GLY241C, GLY244A, GLY249A, GLY249C, GLY251A, GLY251C, GLY253A, GLY253C, GLY259A, GLY259C, GLY280A, GLY280C, GLY282A, GLY282C, GLY289C, GLY28A, GLY61A, GLY61C, HIS240A, HIS240C, HIS93A, HIS93C, ILE222A, ILE222C, ILE223A, ILE223C, ILE238A, ILE238C, ILE245A, ILE245C, ILE283A, ILE283C, ILE305A, ILE305C, ILE60A, ILE60C, LEU217A, LEU217C, LEU70A, LEU70C, LEU73A, LEU73C, LEU98A, LEU98C, LYS168A, LYS168C, LYS178A, LYS178C, LYS216A, LYS239A, LYS239C, LYS243C, LYS257A, LYS257C, LYS302A, LYS302C, MET121A, MET121C, MET233A, MET233C, MET250A, MET250C, MET308A, MET308C, MET65A, MET65C, PHE104A, PHE104C, PHE117A, PHE117C, PHE177A, PHE177C, PRO102A, PRO102C, PRO180A, PRO180C, PRO218C, PRO230A, PRO230C, PRO285A, PRO285C, PRO286C, PRO300A, PRO300C, SER101A, SER101C, SER167A, SER167C, SER172A, SER172C, SER237A, SER237C, SER254A, SER254C, SER256A, SER256C, SER276A, SER276C, SER277A, SER277C, SER281A, SER281C, SER297A, SER297C, SER299A, SER299C, SER62A, SER62C, SER67A, SER67C, THR12C, THR15A, THR169A, THR169C, THR173A, THR173C, THR176A, THR176C, THR26A, THR279A, THR279C, THR27A, THR66A, THR66C, THR95A, THR95C, THR97A, THR97C, TYR103C, TYR224A, TYR224C, TYR226A, TYR226C, TYR232A, TYR232C, TYR234A, TYR234C, TYR247A, TYR247C, TYR29A, VAL220A, VAL220C, VAL242A, VAL242C, VAL246A, VAL246C, VAL255A, VAL255C, VAL284A, VAL284C, VAL294C, VAL69A, VAL69C
• A-D: ALA179A, ALA179D, ALA252A, ALA252D, ALA295A, ALA295D, ARG150A, ARG150D, ARG159A, ARG159D, ARG164A, ARG164D, ARG198A, ARG198D, ARG206A, ARG206D, ASN146A, ASN146D, ASN157A, ASN157D, ASP158A, ASP158D, ASP191A, ASP191D, ASP200A, ASP200D, ASP296A, ASP296D, GLN196A, GLN196D, GLU181A, GLU181D, GLU182A, GLU182D, GLY183A, GLY183D, GLY186A, GLY186D, GLY190A, GLY190D, GLY282A, GLY282D, HIS325A, HIS325D, ILE160A, ILE160D, ILE188A, ILE188D, ILE189A, ILE189D, ILE193A, ILE193D, ILE283A, ILE283D, LEU185A, LEU185D, LEU199A, LEU199D, LEU298A, LEU298D, LYS178A, LYS178D, LYS192A, LYS192D, LYS201A, LYS201D, PHE165A, PHE165D, PHE324A, PHE324D, PRO180A, PRO180D, PRO285A, PRO285D, PRO286A, PRO286D, SER162A, SER162D, SER281A, SER281D, SER297A, SER297D, THR197A, THR197D, TYR184A, TYR184D, TYR194A, TYR194D, TYR195A, TYR195D, TYR327D, VAL187A, VAL187D, VAL284A, VAL284D, VAL294A, VAL294D
• B-C: ALA179B, ALA179C, ALA252B, ALA252C, ALA295B, ALA326C, ARG150B, ARG150C, ARG159B, ARG159C, ARG164B, ARG164C, ARG198B, ARG198C, ARG206B, ASN146B, ASN146C, ASN157B, ASP158B, ASP158C, ASP191B, ASP191C, ASP200B, ASP200C, ASP287C, ASP296B, ASP296C, GLN196B, GLN196C, GLU181B, GLU181C, GLU182B, GLU182C, GLY183B, GLY183C, GLY186B, GLY186C, GLY190B, GLY190C, GLY282B, GLY282C, HIS325B, HIS325C, ILE160B, ILE188B, ILE188C, ILE189B, ILE189C, ILE193B, ILE193C, ILE283B, ILE283C, LEU185B, LEU185C, LEU199B, LEU199C, LEU298B, LEU298C, LYS178B, LYS178C, LYS192B, LYS192C, LYS201B, LYS201C, PHE165B, PHE165C, PHE324B, PHE324C, PRO180B, PRO180C, PRO285B, PRO285C, PRO286B, PRO286C, SER162B, SER162C, SER281B, SER281C, SER297B, SER297C, THR197B, THR197C, TYR184B, TYR184C, TYR194B, TYR194C, TYR195B, TYR195C, TYR327C, VAL187B, VAL187C, VAL284B, VAL284C, VAL294B, VAL294C
• B-D: ALA171B, ALA171D, ALA219B, ALA219D, ALA236B, ALA236D, ALA248B, ALA248D, ALA252B, ALA252D, ALA301B, ALA301D, ARG258B, ARG258D, ARG278B, ARG278D, ARG304B, ARG304D, ASN170B, ASN170D, ASN64B, ASN64D, ASP175B, ASP175D, ASP221B, ASP221D, ASP228B, ASP228D, ASP229B, ASP229D, ASP235B, ASP235D, ASP260B, ASP260D, ASP296B, ASP296D, ASP68B, ASP68D, ASP96B, ASP96D, ASP99B, ASP99D, GLN227B, GLN227D, GLN290B, GLN290D, GLU100B, GLU100D, GLU181B, GLU181D, GLU182B, GLU182D, GLU231D, GLU63B, GLU63D, GLY225B, GLY225D, GLY241B, GLY241D, GLY249B, GLY249D, GLY251B, GLY251D, GLY253B, GLY253D, GLY259B, GLY259D, GLY280B, GLY280D, GLY282B, GLY282D, GLY61B, GLY61D, HIS240B, HIS240D, HIS93B, HIS93D, ILE222B, ILE222D, ILE223B, ILE223D, ILE238B, ILE238D, ILE245B, ILE245D, ILE283B, ILE283D, ILE305B, ILE305D, ILE60B, ILE60D, LEU217B, LEU217D, LEU70B, LEU70D, LEU73B, LEU73D, LEU98B, LEU98D, LYS168B, LYS168D, LYS178B, LYS178D, LYS216B, LYS239B, LYS239D, LYS257B, LYS257D, LYS302B, LYS302D, MET121B, MET121D, MET233B, MET233D, MET250B, MET250D, MET308B, MET308D, MET65B, MET65D, PHE104B, PHE104D, PHE117B, PHE117D, PHE177B, PHE177D, PRO102B, PRO102D, PRO180B, PRO180D, PRO218B, PRO218D, PRO230B, PRO230D, PRO285B, PRO285D, PRO300B, PRO300D, SER101B, SER101D, SER167B, SER167D, SER172B, SER172D, SER237B, SER237D, SER254B, SER254D, SER256B, SER256D, SER276B, SER276D, SER277B, SER277D, SER281B, SER281D, SER297B, SER297D, SER299B, SER299D, SER62B, SER62D, SER67B, SER67D, THR12B, THR15D, THR169B, THR169D, THR173B, THR173D, THR176B, THR176D, THR279B, THR279D, THR66B, THR66D, THR95B, THR95D, THR97B, THR97D, TYR103B, TYR103D, TYR224B, TYR224D, TYR226B, TYR226D, TYR232B, TYR232D, TYR234B, TYR234D, TYR247B, TYR247D, VAL220B, VAL220D, VAL242B, VAL242D, VAL246B, VAL246D, VAL255B, VAL255D, VAL284B, VAL284D, VAL69B, VAL69D
• B-F: ALA318B, ALA318F, ALA326B, ALA326F, ARG148B, ARG148F, ARG206B, ARG206F, ASN316B, ASN316F, ASP112B, ASP112F, ASP200F, ASP210B, ASP210F, ASP322B, ASP322F, GLN321B, GLN321F, HIS203B, HIS203F, HIS325B, HIS325F, ILE320B, ILE320F, LYS110B, LYS110F, LYS201B, LYS313B, LYS313F, PHE209B, PHE209F, PRO317B, PRO317F, SER207B, SER207F, THR204B, THR204F, THR205B, THR205F, THR314B, THR314F, TYR323B, TYR323F, TYR327B, TYR327F, VAL109B, VAL202B, VAL202F, VAL208B, VAL208F, VAL319B, VAL319F
• B-G: ALA82B, ARG148G, ARG150G, ARG198G, ARG206G, ARG83B, ASN146G, ASN213B, ASN3G, ASP112G, ASP144G, ASP210B, ASP215B, ASP87G, GLU79B, GLY149G, LYS110B, LYS113G, LYS145G, LYS216B, LYS243B, LYS313B, LYS75B, PRO114G, SER147G, SER84B, THR212B, THR312B, VAL211B, VAL214B
• C-D: ALA120D, ALA126C, ALA126D, ALA129C, ALA129D, ALA171C, ALA171D, ALA21C, ALA22C, ALA22D, ALA42C, ALA42D, ARG122C, ARG122D, ARG159C, ARG159D, ASN134C, ASN134D, ASN157C, ASN157D, ASN170C, ASN170D, ASN23D, ASP130C, ASP130D, ASP158C, ASP158D, ASP175C, ASP175D, ASP191C, ASP191D, GLN25D, GLU45C, GLU45D, GLY131C, GLY131D, GLY137C, GLY137D, GLY190C, GLY190D, ILE127C, ILE127D, ILE188C, ILE188D, ILE189C, ILE189D, LEU135C, LEU135D, LEU156C, LEU156D, LEU174C, LEU174D, LEU46C, LEU46D, LEU49C, LEU49D, LYS140C, LYS178C, LYS178D, MET121C, MET121D, MET133C, MET133D, PRO123C, PRO123D, PRO132C, PRO132D, PRO44C, PRO44D, SER128C, SER128D, SER172C, SER172D, THR125C, THR169C, THR169D, THR173C, THR173D, THR176C, THR176D, THR24D, THR48C, TYR136C, TYR136D, TYR184C, TYR184D, TYR194C, VAL187C, VAL187D, VAL43C, VAL43D
• C-F: ARG148C, ARG150C, ARG198C, ASN146C, ASN213F, ASN3C, ASP112C, ASP144C, ASP210F, ASP215F, ASP87C, GLY149C, LEU217F, LEU311F, LYS113C, LYS145C, LYS216F, LYS243F, LYS313F, LYS75F, PRO114C, PRO218F, SER147C, THR205C, THR212F, THR312F, VAL211F, VAL214F
• E-F: ALA120F, ALA126E, ALA126F, ALA129E, ALA129F, ALA171E, ALA171F, ALA22F, ALA42E, ALA42F, ARG122E, ARG122F, ARG159E, ARG159F, ASN134E, ASN134F, ASN157E, ASN157F, ASN170E, ASN170F, ASN23F, ASP130E, ASP130F, ASP158E, ASP158F, ASP175E, ASP175F, ASP191E, GLN196E, GLN196F, GLU45E, GLU45F, GLY131E, GLY131F, GLY137E, GLY137F, GLY190E, GLY190F, ILE127E, ILE127F, ILE188E, ILE188F, ILE189E, ILE189F, LEU135E, LEU135F, LEU156E, LEU156F, LEU174E, LEU174F, LEU46E, LEU46F, LEU49E, LEU49F, LYS140E, LYS178E, LYS178F, MET121E, MET121F, MET133E, MET133F, PRO123E, PRO123F, PRO132E, PRO132F, PRO44E, PRO44F, SER128E, SER128F, SER172E, SER172F, THR169E, THR169F, THR173E, THR173F, THR176E, THR176F, THR24F, THR48E, TYR136E, TYR136F, TYR184E, TYR184F, VAL187E, VAL187F, VAL43E, VAL43F
• E-G: ALA171E, ALA171G, ALA219E, ALA219G, ALA236E, ALA236G, ALA248E, ALA248G, ALA252E, ALA252G, ALA301E, ALA301G, ARG258E, ARG258G, ARG278E, ARG278G, ARG304E, ARG304G, ASN170E, ASN170G, ASN64E, ASN64G, ASP175E, ASP175G, ASP221E, ASP221G, ASP228E, ASP228G, ASP229E, ASP229G, ASP235E, ASP235G, ASP260E, ASP260G, ASP296E, ASP296G, ASP68E, ASP68G, ASP96E, ASP96G, ASP99E, ASP99G, GLN227E, GLN227G, GLN290E, GLN290G, GLU100E, GLU100G, GLU181E, GLU181G, GLU182E, GLU182G, GLU231E, GLU231G, GLU63E, GLU63G, GLY225E, GLY225G, GLY241E, GLY241G, GLY244E, GLY249E, GLY249G, GLY251E, GLY251G, GLY253E, GLY253G, GLY259E, GLY259G, GLY280E, GLY280G, GLY282E, GLY282G, GLY61E, GLY61G, HIS240E, HIS240G, HIS93E, HIS93G, ILE222E, ILE222G, ILE223E, ILE223G, ILE238E, ILE238G, ILE245E, ILE245G, ILE271E, ILE283E, ILE283G, ILE305E, ILE305G, ILE60E, ILE60G, LEU217E, LEU217G, LEU70E, LEU70G, LEU73G, LEU98E, LEU98G, LYS168E, LYS168G, LYS178E, LYS178G, LYS239E, LYS239G, LYS243E, LYS257E, LYS257G, LYS302E, LYS302G, MET121E, MET121G, MET233E, MET233G, MET250E, MET250G, MET308E, MET308G, MET65E, MET65G, PHE104E, PHE104G, PHE117E, PHE117G, PHE177E, PHE177G, PRO102E, PRO102G, PRO180E, PRO180G, PRO218E, PRO230E, PRO230G, PRO285E, PRO285G, PRO300E, PRO300G, SER101E, SER101G, SER167E, SER167G, SER172E, SER172G, SER237E, SER237G, SER254E, SER254G, SER256E, SER256G, SER276E, SER276G, SER277E, SER277G, SER281E, SER281G, SER297E, SER297G, SER299E, SER299G, SER62E, SER62G, SER67E, SER67G, THR12E, THR12G, THR15G, THR169E, THR169G, THR173E, THR173G, THR176E, THR176G, THR279E, THR279G, THR66E, THR66G, THR95E, THR95G, THR97E, THR97G, TYR103E, TYR103G, TYR224E, TYR224G, TYR226E, TYR226G, TYR232E, TYR232G, TYR234E, TYR234G, TYR247E, TYR247G, VAL220E, VAL220G, VAL242E, VAL242G, VAL246E, VAL246G, VAL255E, VAL255G, VAL284E, VAL284G, VAL69E, VAL69G
• E-H: ALA179E, ALA179H, ALA252E, ALA252H, ALA295H, ALA326H, ARG150E, ARG150H, ARG159E, ARG159H, ARG164E, ARG164H, ARG198E, ARG198H, ASN146E, ASN146H, ASN157E, ASN157H, ASP158E, ASP158H, ASP191E, ASP191H, ASP200E, ASP200H, ASP287H, ASP296E, ASP296H, GLN196E, GLN196H, GLN321H, GLU181E, GLU181H, GLU182E, GLU182H, GLY183E, GLY183H, GLY186E, GLY186H, GLY190E, GLY190H, GLY282E, GLY282H, HIS325E, HIS325H, ILE160E, ILE160H, ILE188E, ILE188H, ILE189E, ILE189H, ILE193E, ILE193H, ILE283E, ILE283H, LEU185E, LEU185H, LEU199E, LEU199H, LEU298H, LYS178E, LYS178H, LYS192E, LYS192H, LYS201E, LYS201H, PHE165E, PHE165H, PHE324E, PHE324H, PRO180E, PRO180H, PRO285E, PRO285H, PRO286E, PRO286H, SER162E, SER162H, SER281E, SER281H, SER297E, SER297H, THR197E, THR197H, TYR184E, TYR184H, TYR194E, TYR194H, TYR195E, TYR195H, VAL187E, VAL187H, VAL284E, VAL284H, VAL294E, VAL294H
• F-G: ALA179F, ALA179G, ALA252F, ALA252G, ALA295F, ALA326F, ARG150F, ARG150G, ARG159F, ARG159G, ARG164F, ARG164G, ARG198F, ARG198G, ASN146F, ASN146G, ASN157F, ASP158F, ASP158G, ASP191F, ASP191G, ASP200F, ASP200G, ASP287F, ASP287G, ASP296F, ASP296G, GLN196F, GLN196G, GLU181F, GLU181G, GLU182F, GLU182G, GLY183F, GLY183G, GLY186F, GLY186G, GLY190F, GLY190G, GLY282F, GLY282G, HIS203F, HIS325F, HIS325G, ILE160F, ILE188F, ILE188G, ILE189F, ILE189G, ILE193F, ILE193G, ILE283F, ILE283G, LEU185F, LEU185G, LEU199F, LEU199G, LEU298G, LYS178F, LYS178G, LYS192F, LYS192G, LYS201F, LYS201G, PHE165F, PHE165G, PHE324F, PHE324G, PRO180F, PRO180G, PRO285F, PRO285G, PRO286F, PRO286G, SER162F, SER162G, SER281F, SER281G, SER297F, SER297G, THR197F, THR197G, TYR184F, TYR184G, TYR194F, TYR194G, TYR195F, TYR195G, VAL187F, VAL187G, VAL284F, VAL284G, VAL294F, VAL294G
• F-H: ALA171F, ALA171H, ALA219F, ALA219H, ALA236F, ALA236H, ALA248F, ALA248H, ALA252F, ALA252H, ALA301F, ALA301H, ARG258F, ARG258H, ARG278F, ARG278H, ARG304F, ARG304H, ASN170F, ASN170H, ASN64F, ASN64H, ASP175F, ASP175H, ASP221F, ASP221H, ASP228F, ASP228H, ASP229F, ASP229H, ASP235F, ASP235H, ASP260F, ASP260H, ASP296F, ASP296H, ASP68F, ASP68H, ASP96F, ASP96H, ASP99F, ASP99H, GLN227F, GLN227H, GLN290F, GLN290H, GLU100F, GLU100H, GLU181F, GLU181H, GLU182F, GLU182H, GLU231F, GLU231H, GLU63F, GLU63H, GLY225F, GLY225H, GLY241F, GLY241H, GLY249F, GLY249H, GLY251F, GLY251H, GLY253F, GLY253H, GLY259F, GLY259H, GLY280F, GLY280H, GLY282F, GLY282H, GLY61F, GLY61H, HIS240F, HIS240H, HIS93F, HIS93H, ILE222F, ILE222H, ILE223F, ILE223H, ILE238F, ILE238H, ILE245F, ILE245H, ILE283F, ILE283H, ILE305F, ILE305H, ILE60F, ILE60H, LEU217F, LEU217H, LEU70F, LEU70H, LEU73F, LEU73H, LEU98F, LEU98H, LYS168F, LYS168H, LYS178F, LYS178H, LYS216F, LYS239F, LYS239H, LYS243H, LYS257F, LYS257H, LYS302F, LYS302H, MET121F, MET121H, MET233F, MET233H, MET250F, MET250H, MET308F, MET308H, MET65F, MET65H, PHE104F, PHE104H, PHE117F, PHE117H, PHE177F, PHE177H, PRO102F, PRO102H, PRO180F, PRO180H, PRO218F, PRO230F, PRO230H, PRO285F, PRO285H, PRO300F, PRO300H, SER101F, SER101H, SER167F, SER167H, SER172F, SER172H, SER237F, SER237H, SER254F, SER254H, SER256F, SER256H, SER276F, SER276H, SER277F, SER277H, SER281F, SER281H, SER297F, SER297H, SER299F, SER299H, SER62F, SER62H, SER67F, SER67H, THR12F, THR12H, THR15F, THR169F, THR169H, THR173F, THR173H, THR176F, THR176H, THR279F, THR279H, THR66F, THR66H, THR95F, THR95H, THR97F, THR97H, TYR103F, TYR103H, TYR224F, TYR224H, TYR226F, TYR226H, TYR232F, TYR232H, TYR234F, TYR234H, TYR247F, TYR247H, VAL220F, VAL220H, VAL242F, VAL242H, VAL246F, VAL246H, VAL255F, VAL255H, VAL284F, VAL284H, VAL69F, VAL69H
• G-H: ALA120G, ALA126G, ALA126H, ALA129G, ALA129H, ALA171G, ALA171H, ALA42G, ALA42H, ARG122G, ARG122H, ARG159G, ARG159H, ASN134G, ASN134H, ASN157G, ASN157H, ASN170G, ASN170H, ASP130G, ASP130H, ASP158G, ASP158H, ASP175G, ASP175H, GLN196G, GLN196H, GLU45G, GLU45H, GLY131G, GLY131H, GLY137G, GLY137H, GLY190G, GLY190H, ILE127G, ILE127H, ILE188G, ILE188H, ILE189G, ILE189H, LEU135G, LEU135H, LEU156G, LEU156H, LEU174G, LEU174H, LEU46G, LEU46H, LEU49G, LYS178G, LYS178H, MET121G, MET121H, MET133G, MET133H, PRO123G, PRO132G, PRO132H, PRO44G, PRO44H, SER128G, SER128H, SER172G, SER172H, THR125H, THR169G, THR169H, THR173G, THR173H, THR176G, THR176H, TYR136G, TYR136H, TYR184G, TYR184H, TYR194H, VAL187G, VAL187H, VAL43G, VAL43H