2J6X

the crystal structure of lactate oxidase

• Total interactions analyzed: 28
• Total true interactions: 18
• Strongest Interaction:
  • Chains: F-H
  • Int. Res.: 147
  • Norm. En. per Res.: -6.0387
• Hub Node: A(5)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
A-B	-142.8331	-172.3081	-569.688	-884.8292	152	25	16	20038	12	69	56
A-C	-135.3205	-172.5885	-576.394	-884.303	150	34	15	20302	12	69	57
A-D	0.0	6.9259	-0.2421	6.6839	3	0	0	41	0	5	1
A-E	0.0	-13.2878	-5.1926	-18.4804	19	0	0	438	0	13	14
A-F	-14.8925	-46.911	-32.4476	-94.2511	36	2	0	1611	4	29	26
B-C	0.0	5.533	-0.1233	5.4097	6	0	0	35	0	4	1
B-D	-118.3919	-157.7523	-574.979	-851.1232	150	23	15	20155	12	65	57
B-G	0.0	6.2556	-15.0052	-8.7496	14	3	0	1084	0	9	18
C-D	-148.8715	-143.5064	-584.435	-876.8129	151	22	15	20333	13	68	56
C-F	0.0	2.424	-0.5006	1.9234	11	0	0	129	0	8	6
C-H	-10.8185	-48.1889	-33.3315	-92.3388	36	2	0	1688	4	33	25
D-H	0.0	-13.0837	-15.4185	-28.5022	24	0	0	860	0	15	15
E-F	-142.6304	-179.6996	-575.705	-898.035	154	25	16	20155	15	67	56
E-G	-160.5398	-178.5545	-576.336	-915.4303	152	24	15	20098	13	65	56
E-H	0.0	6.3644	-0.1485	6.216	5	0	0	35	0	4	1
F-G	0.0	6.035	-0.1739	5.8611	5	0	0	38	0	4	1
F-H	-143.5172	-174.6325	-569.534	-887.6837	147	27	15	20332	13	69	57
G-H	-129.0226	-136.7016	-561.49	-827.2141	149	24	15	19966	9	68	56

Interface residues

• A-B: ALA173A, ALA249A, ALA252A, ALA255A, ALA267A, ALA273A, ALA282A, ALA306B, ALA53A, ALA57A, ALA57B, ALA68B, ALA73B, ARG181A, ARG183A, ARG225A, ARG268A, ARG286A, ARG300B, ARG301B, ARG347B, ARG52A, ARG56A, ARG56B, ARG66B, ASN180A, ASN187A, ASN21B, ASN264A, ASN368A, ASN368B, ASN54A, ASP174A, ASP182A, ASP18B, ASP226A, ASP248A, ASP24B, ASP250A, ASP278A, ASP344B, ASP364B, ASP367A, ASP367B, ASP47A, ASP55A, ASP55B, ASP70B, GLN220A, GLN244A, GLN269A, GLN350B, GLN355B, GLN69B, GLU228A, GLU247A, GLU272A, GLU28B, GLU303B, GLU373A, GLU373B, GLU48A, GLU72B, GLY179A, GLY242A, GLY275A, GLY302B, GLY353B, GLY371A, GLY371B, GLY46A, HIS245A, HIS265A, HIS304B, HIS60A, HIS60B, ILE222A, ILE227A, ILE243A, ILE260A, ILE283A, LEU171A, LEU270A, LEU280A, LEU351B, LEU363B, LEU365B, LEU62B, LEU63B, LEU67B, LYS149A, LYS186A, LYS188A, LYS219A, LYS221A, LYS241A, LYS307B, LYS343B, LYS59A, LYS59B, LYS61A, LYS61B, MET147A, MET251A, MET349B, PHE277A, PHE366B, PRO224A, PRO246A, PRO274A, PRO281A, PRO369A, PRO369B, PRO65B, PRO74B, SER175A, SER178A, SER218A, SER223A, SER263A, SER276A, SER45A, THR172A, THR176A, THR22B, THR279A, THR346B, THR352B, THR50A, TRP261A, TRP49A, TRP58A, TRP58B, TYR215A, TYR23B, TYR271A, TYR340B, TYR370A, TYR370B, TYR372A, TYR372B, TYR374A, TYR374B, TYR64B, VAL177A, VAL185A, VAL19B, VAL20B, VAL240A, VAL262A, VAL348B, VAL71B
• A-C: ALA173C, ALA249C, ALA252C, ALA255C, ALA267C, ALA273C, ALA282C, ALA306A, ALA53C, ALA57A, ALA57C, ALA68A, ALA73A, ARG181C, ARG183C, ARG225C, ARG268C, ARG300A, ARG301A, ARG347A, ARG52C, ARG56A, ARG56C, ARG66A, ASN180C, ASN187C, ASN212C, ASN21A, ASN264C, ASN368A, ASN368C, ASN54C, ASP174C, ASP182C, ASP184C, ASP18A, ASP226C, ASP248C, ASP24A, ASP250C, ASP278C, ASP344A, ASP364A, ASP367A, ASP367C, ASP47C, ASP55A, ASP55C, ASP70A, GLN220C, GLN244C, GLN269C, GLN350A, GLN355A, GLN69A, GLU247C, GLU272C, GLU303A, GLU373A, GLU373C, GLU48C, GLU72A, GLY179C, GLY242C, GLY275C, GLY302A, GLY353A, GLY371A, GLY371C, GLY46C, HIS245C, HIS265C, HIS304A, HIS60A, HIS60C, ILE222C, ILE227C, ILE243C, ILE260C, ILE283C, LEU171C, LEU270C, LEU280C, LEU351A, LEU363A, LEU365A, LEU62A, LEU63A, LEU67A, LYS149C, LYS186C, LYS188C, LYS219C, LYS221C, LYS241C, LYS307A, LYS343A, LYS59A, LYS59C, LYS61A, LYS61C, MET251C, MET349A, PHE277A, PHE277C, PHE366A, PRO224C, PRO246C, PRO274C, PRO281C, PRO369A, PRO369C, PRO65A, PRO74A, SER175C, SER178C, SER218C, SER223C, SER263C, SER276C, SER45C, THR172C, THR176C, THR279C, THR346A, THR352A, THR50C, TRP261C, TRP49C, TRP58A, TRP58C, TYR215C, TYR23A, TYR271C, TYR340A, TYR370A, TYR370C, TYR372A, TYR372C, TYR374A, TYR374C, TYR64A, VAL177C, VAL185C, VAL19A, VAL20A, VAL240C, VAL262C, VAL348A, VAL71A
• A-D: ARG52D, GLU373A, GLU373D
• A-E: ALA232A, ALA255A, ALA282E, ARG225A, ARG286E, ARG290E, ASN288E, ASP250E, GLU228A, GLU229A, GLU285E, GLY256A, ILE253A, LYS254A, LYS254E, LYS289E, PRO246E, PRO281E, VAL287E
• A-F: ALA255F, ALA282F, ARG286F, ARG290A, ARG290F, ASN288A, ASN288F, ASN356A, ASP250F, ASP359A, ASP367F, ASP70A, GLN355A, GLN69A, GLU247F, GLU285F, GLU358A, GLU79A, GLY353A, GLY362A, ILE253F, LEU360A, LEU363A, LYS254F, LYS289A, LYS289F, LYS361A, LYS61F, LYS84A, PRO281F, SER354A, SER77A, THR78A, VAL287F, VAL357A, VAL71A
• B-C: ARG52B, ARG52C, GLU373B, GLU373C, TRP49C, TYR374B
• B-D: ALA173B, ALA249B, ALA252B, ALA255B, ALA267B, ALA273B, ALA282B, ALA306D, ALA53B, ALA57B, ALA57D, ALA68D, ALA73D, ARG181B, ARG183B, ARG225B, ARG268B, ARG286B, ARG300D, ARG301D, ARG347D, ARG52B, ARG56B, ARG56D, ARG66D, ASN180B, ASN187B, ASN212B, ASN21D, ASN264B, ASN368B, ASN368D, ASN54B, ASP174B, ASP182B, ASP18D, ASP226B, ASP248B, ASP24D, ASP250B, ASP278B, ASP344D, ASP364D, ASP367B, ASP367D, ASP47B, ASP55B, ASP55D, ASP70D, GLN220B, GLN244B, GLN269B, GLN350D, GLN355D, GLN69D, GLU247B, GLU272B, GLU28D, GLU303D, GLU373B, GLU373D, GLU48B, GLU72D, GLY179B, GLY242B, GLY275B, GLY302D, GLY353D, GLY371B, GLY371D, GLY46B, HIS245B, HIS265B, HIS304D, HIS60B, HIS60D, ILE222B, ILE227B, ILE243B, ILE260B, ILE283B, LEU171B, LEU270B, LEU280B, LEU351D, LEU363D, LEU365D, LEU62D, LEU63D, LEU67D, LYS149B, LYS186B, LYS188B, LYS219B, LYS221B, LYS241B, LYS307D, LYS343D, LYS59B, LYS59D, LYS61B, LYS61D, MET251B, MET349D, PHE277B, PHE366D, PRO224B, PRO246B, PRO274B, PRO281B, PRO369B, PRO369D, PRO65D, PRO74D, SER175B, SER178B, SER218B, SER223B, SER263B, SER276B, SER45B, THR172B, THR176B, THR279B, THR346D, THR352D, THR50B, TRP261B, TRP49B, TRP58B, TRP58D, TYR215B, TYR23D, TYR271B, TYR340D, TYR370B, TYR370D, TYR372B, TYR372D, TYR374B, TYR374D, TYR64D, VAL177B, VAL185B, VAL19D, VAL20D, VAL240B, VAL262B, VAL348D, VAL71D
• B-G: ALA284G, ARG286B, ARG286G, ARG290B, ARG290G, ASN288B, ASN288G, ASP250B, GLU285B, GLU285G, LYS289B, LYS289G, VAL287B, VAL287G
• C-D: ALA173D, ALA249D, ALA252D, ALA255D, ALA273D, ALA282D, ALA306C, ALA53D, ALA57C, ALA57D, ALA68C, ALA73C, ARG181D, ARG183D, ARG225D, ARG268D, ARG286D, ARG300C, ARG301C, ARG347C, ARG52D, ARG56C, ARG56D, ARG66C, ASN180D, ASN187D, ASN21C, ASN264D, ASN368C, ASN368D, ASN54D, ASP174D, ASP182D, ASP184D, ASP18C, ASP226D, ASP248D, ASP24C, ASP250D, ASP278D, ASP344C, ASP364C, ASP367C, ASP367D, ASP47D, ASP55C, ASP55D, ASP70C, GLN220D, GLN244D, GLN269D, GLN350C, GLN355C, GLN69C, GLU228D, GLU247D, GLU272D, GLU28C, GLU303C, GLU373C, GLU373D, GLU48D, GLU72C, GLY179D, GLY242D, GLY275D, GLY302C, GLY353C, GLY371C, GLY371D, GLY46D, HIS245D, HIS265D, HIS304C, HIS60C, HIS60D, ILE222D, ILE227D, ILE243D, ILE260D, ILE283D, LEU171D, LEU270D, LEU280D, LEU351C, LEU363C, LEU365C, LEU62C, LEU63C, LEU67C, LYS149D, LYS186D, LYS188D, LYS219D, LYS221D, LYS241D, LYS307C, LYS343C, LYS59C, LYS59D, LYS61C, LYS61D, MET251D, MET349C, PHE277C, PHE277D, PHE366C, PRO224D, PRO246D, PRO274D, PRO281D, PRO369C, PRO369D, PRO65C, PRO74C, SER175D, SER178D, SER218D, SER223D, SER263D, SER276D, SER45D, THR172D, THR176D, THR279D, THR346C, THR352C, THR50D, TRP261D, TRP49D, TRP58C, TRP58D, TYR215D, TYR23C, TYR271D, TYR340C, TYR370C, TYR370D, TYR372C, TYR372D, TYR374C, TYR374D, TYR64C, VAL177D, VAL185D, VAL19C, VAL20C, VAL240D, VAL262D, VAL348C, VAL71C
• C-F: ALA255F, ARG225C, ARG225F, GLU228C, GLU228F, LYS221C, LYS221F, LYS254C, LYS254F, PRO224C, PRO224F
• C-H: ALA255C, ALA282C, ARG286C, ARG290C, ARG290H, ASN288C, ASN288H, ASN356H, ASP250C, ASP359H, ASP367C, ASP70H, GLN355H, GLN69H, GLU247C, GLU285C, GLU358H, GLU79H, GLY353H, GLY362H, ILE253C, LEU360H, LEU363H, LYS254C, LYS289C, LYS289H, LYS361H, LYS61C, LYS84H, PRO281C, SER354H, SER77H, THR78H, VAL287C, VAL357H, VAL71H
• D-H: ALA231H, ALA232H, ALA255H, ALA282D, ALA284D, ARG225H, ARG286D, ARG290D, ASN288D, ASP250D, GLU228H, GLU229H, GLU247D, GLU285D, GLY256H, HIS233H, ILE253H, LYS254D, LYS254H, LYS289D, LYS61D, PRO246D, PRO281D, VAL287D
• E-F: ALA173E, ALA249E, ALA252E, ALA255E, ALA267E, ALA273E, ALA282E, ALA306F, ALA53E, ALA57E, ALA57F, ALA68F, ALA73F, ARG181E, ARG183E, ARG225E, ARG268E, ARG286E, ARG300F, ARG301F, ARG347F, ARG52E, ARG56E, ARG56F, ARG66F, ASN180E, ASN187E, ASN21F, ASN264E, ASN368E, ASN368F, ASN54E, ASP174E, ASP182E, ASP184E, ASP18F, ASP226E, ASP248E, ASP24F, ASP250E, ASP278E, ASP344F, ASP364F, ASP367E, ASP367F, ASP47E, ASP55E, ASP55F, ASP70F, GLN220E, GLN244E, GLN269E, GLN350F, GLN355F, GLN69F, GLU228E, GLU247E, GLU272E, GLU28F, GLU303F, GLU373E, GLU373F, GLU48E, GLU72F, GLY179E, GLY216E, GLY242E, GLY275E, GLY302F, GLY353F, GLY371E, GLY371F, GLY46E, HIS245E, HIS265E, HIS304F, HIS60E, HIS60F, ILE222E, ILE227E, ILE243E, ILE260E, ILE283E, LEU171E, LEU270E, LEU280E, LEU351F, LEU363F, LEU365F, LEU62F, LEU63F, LEU67F, LYS149E, LYS186E, LYS188E, LYS219E, LYS221E, LYS241E, LYS307F, LYS343F, LYS59E, LYS59F, LYS61E, LYS61F, MET147E, MET251E, MET349F, PHE277E, PHE366F, PRO224E, PRO246E, PRO274E, PRO281E, PRO369E, PRO369F, PRO65F, PRO74F, SER175E, SER178E, SER218E, SER223E, SER263E, SER276E, SER45E, THR172E, THR176E, THR22F, THR279E, THR346F, THR352F, THR50E, TRP261E, TRP49E, TRP58E, TRP58F, TYR215E, TYR23F, TYR271E, TYR340F, TYR370E, TYR370F, TYR372E, TYR372F, TYR374E, TYR374F, TYR64F, VAL177E, VAL185E, VAL19F, VAL20F, VAL240E, VAL262E, VAL348F, VAL71F
• E-G: ALA173G, ALA249G, ALA252G, ALA255G, ALA267G, ALA273G, ALA282G, ALA306E, ALA53G, ALA57E, ALA57G, ALA68E, ALA73E, ARG181G, ARG183G, ARG225G, ARG268G, ARG286G, ARG300E, ARG301E, ARG347E, ARG52G, ARG56E, ARG56G, ARG66E, ASN180G, ASN187G, ASN212G, ASN21E, ASN264G, ASN368E, ASN368G, ASN54G, ASP174G, ASP182G, ASP184G, ASP18E, ASP226G, ASP248G, ASP24E, ASP250G, ASP278G, ASP344E, ASP364E, ASP367E, ASP367G, ASP47G, ASP55E, ASP55G, ASP70E, GLN220G, GLN244G, GLN269G, GLN350E, GLN355E, GLN69E, GLU247G, GLU272G, GLU28E, GLU303E, GLU373E, GLU373G, GLU48G, GLU72E, GLY179G, GLY242G, GLY275G, GLY302E, GLY353E, GLY371E, GLY371G, GLY46G, HIS245G, HIS265G, HIS304E, HIS60E, HIS60G, ILE222G, ILE227G, ILE243G, ILE260G, ILE283G, LEU171G, LEU270G, LEU280G, LEU351E, LEU363E, LEU365E, LEU62E, LEU63E, LEU67E, LYS149G, LYS186G, LYS188G, LYS219G, LYS221G, LYS241G, LYS307E, LYS343E, LYS59E, LYS59G, LYS61E, LYS61G, MET251G, MET349E, PHE277E, PHE277G, PHE366E, PRO224G, PRO246G, PRO274G, PRO281G, PRO369E, PRO369G, PRO65E, PRO74E, SER175G, SER178G, SER218G, SER223G, SER263G, SER276G, SER45G, THR172G, THR176G, THR279G, THR346E, THR352E, THR50G, TRP261G, TRP49G, TRP58E, TRP58G, TYR215G, TYR23E, TYR271G, TYR340E, TYR370E, TYR370G, TYR372E, TYR372G, TYR374E, TYR374G, TYR64E, VAL177G, VAL185G, VAL19E, VAL20E, VAL240G, VAL262G, VAL348E, VAL71E
• E-H: ARG52E, ARG52H, ARG56H, GLU373E, GLU373H
• F-G: ARG52F, ARG52G, ARG56F, GLU373F, GLU373G
• F-H: ALA173F, ALA249F, ALA252F, ALA255F, ALA273F, ALA282F, ALA306H, ALA53F, ALA57F, ALA68H, ALA73H, ARG181F, ARG183F, ARG225F, ARG268F, ARG300H, ARG301H, ARG347H, ARG52F, ARG56F, ARG56H, ARG66H, ASN180F, ASN187F, ASN212F, ASN21H, ASN264F, ASN368F, ASN368H, ASN54F, ASP174F, ASP182F, ASP184F, ASP18H, ASP226F, ASP248F, ASP24H, ASP250F, ASP278F, ASP344H, ASP364H, ASP367F, ASP367H, ASP47F, ASP55F, ASP55H, ASP70H, GLN220F, GLN244F, GLN269F, GLN350H, GLN355H, GLN69H, GLU247F, GLU272F, GLU303H, GLU373F, GLU373H, GLU48F, GLU72H, GLY179F, GLY242F, GLY275F, GLY302H, GLY353H, GLY371F, GLY371H, GLY46F, HIS245F, HIS265F, HIS304H, HIS60F, HIS60H, ILE222F, ILE227F, ILE243F, ILE260F, ILE283F, LEU171F, LEU270F, LEU280F, LEU351H, LEU363H, LEU365H, LEU62H, LEU63H, LEU67H, LYS149F, LYS186F, LYS188F, LYS219F, LYS221F, LYS241F, LYS307H, LYS343H, LYS59F, LYS59H, LYS61F, LYS61H, MET251F, MET349H, PHE277F, PHE366H, PRO224F, PRO246F, PRO274F, PRO281F, PRO369F, PRO369H, PRO65H, PRO74H, SER175F, SER178F, SER218F, SER223F, SER263F, SER276F, SER45F, THR172F, THR176F, THR279F, THR346H, THR352H, THR50F, TRP261F, TRP49F, TRP58F, TRP58H, TYR215F, TYR23H, TYR271F, TYR340H, TYR370F, TYR370H, TYR372F, TYR372H, TYR374F, TYR374H, TYR64H, VAL177F, VAL185F, VAL19H, VAL20H, VAL240F, VAL262F, VAL348H, VAL71H
• G-H: ALA173H, ALA249H, ALA252H, ALA255H, ALA267H, ALA273H, ALA282H, ALA306G, ALA53H, ALA57G, ALA57H, ALA68G, ALA73G, ARG181H, ARG183H, ARG225H, ARG268H, ARG286H, ARG300G, ARG301G, ARG347G, ARG52H, ARG56G, ARG56H, ARG66G, ASN180H, ASN187H, ASN21G, ASN264H, ASN368G, ASN368H, ASN54H, ASP174H, ASP182H, ASP184H, ASP18G, ASP226H, ASP248H, ASP24G, ASP250H, ASP278H, ASP344G, ASP364G, ASP367G, ASP367H, ASP47H, ASP55G, ASP55H, ASP70G, GLN220H, GLN244H, GLN269H, GLN350G, GLN355G, GLN69G, GLU247H, GLU272H, GLU28G, GLU303G, GLU373G, GLU373H, GLU48H, GLU72G, GLY179H, GLY242H, GLY275H, GLY302G, GLY353G, GLY371G, GLY371H, GLY46H, HIS245H, HIS265H, HIS304G, HIS60G, HIS60H, ILE222H, ILE227H, ILE243H, ILE260H, ILE283H, LEU171H, LEU270H, LEU280H, LEU351G, LEU363G, LEU365G, LEU62G, LEU63G, LEU67G, LYS149H, LYS186H, LYS188H, LYS219H, LYS221H, LYS241H, LYS307G, LYS343G, LYS59G, LYS59H, LYS61G, LYS61H, MET251H, MET349G, PHE277H, PHE366G, PRO224H, PRO246H, PRO274H, PRO281H, PRO369G, PRO369H, PRO65G, PRO74G, SER175H, SER178H, SER218H, SER223H, SER263H, SER276H, SER45H, THR172H, THR176H, THR279H, THR346G, THR352G, THR50H, TRP261H, TRP49H, TRP58G, TRP58H, TYR23G, TYR271H, TYR340G, TYR370G, TYR370H, TYR372G, TYR372H, TYR374G, TYR374H, TYR64G, VAL177H, VAL185H, VAL19G, VAL20G, VAL240H, VAL262H, VAL348G, VAL71G