2IUO

site directed mutagenesis of key residues involved in the catalytic mechanism of cyanase

• Total interactions analyzed: 45
• Total true interactions: 40
• Strongest Interaction:
  • Chains: B-F
  • Int. Res.: 199
  • Norm. En. per Res.: -6.6866
• Hub Node: A(8)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
A-B	-28.8697	-49.9231	-165.403	-244.1958	90	8	3	7349	3	26	13
A-C	0.0	0.0	-0.015	-0.015	2	0	0	15	0	0	0
A-D	-262.2058	-73.2732	-960.416	-1295.8949	198	45	43	38847	9	81	62
A-E	0.0	0.0	-0.0022	-0.0022	2	0	0	1	0	1	1
A-F	-17.7412	18.2874	-82.9494	-82.4032	27	0	1	2726	8	6	7
A-H	0.0	0.0	-0.0026	-0.0026	2	0	0	1	0	1	1
A-I	-24.314	-49.6569	-174.646	-248.617	89	9	4	7765	4	25	16
A-J	-51.3925	0.0	-375.138	-426.5305	131	17	10	14988	6	11	19
B-D	-58.165	11.1677	-347.23	-394.2273	129	10	9	14474	6	10	19
B-E	-38.9937	-48.1466	-175.227	-262.3673	91	11	3	7733	3	26	14
B-F	-271.694	-75.9461	-982.987	-1330.6271	199	59	43	39356	9	77	64
B-G	-15.4259	18.3423	-81.3804	-78.464	26	0	1	2728	8	6	6
B-H	0.0	0.0	-0.0027	-0.0027	2	0	0	1	0	1	1
B-I	0.0	0.0	-0.0023	-0.0023	2	0	0	1	0	1	1
B-J	0.0	0.0	-0.0148	-0.0148	2	0	0	15	0	0	0
C-D	0.0	0.0	-0.0027	-0.0027	2	0	0	1	0	1	1
C-E	-17.3182	11.8645	-82.7503	-88.204	27	0	1	2751	8	6	8
C-F	0.0	0.0	-0.0031	-0.0031	2	0	0	1	0	1	1
C-G	-20.0081	-46.9253	-172.789	-239.7224	90	6	4	7727	3	26	16
C-H	-249.0403	-74.3551	-963.256	-1286.6513	197	57	42	38908	9	81	63
C-I	-57.9986	10.9007	-340.706	-387.8039	132	18	10	14407	7	11	19
C-J	-24.2483	-46.8666	-166.688	-237.8029	89	7	3	7432	4	27	16
D-E	0.0	0.0	-0.0168	-0.0168	2	0	0	16	0	0	0
D-F	-34.5829	-45.1329	-166.16	-245.8758	90	8	3	7481	2	27	13
D-G	0.0	0.0	-0.0024	-0.0024	2	0	0	1	0	1	1
D-I	-19.4882	-75.4732	-82.6741	-177.6355	27	0	1	2764	8	6	6
D-J	-36.5949	-46.5758	-166.061	-249.2317	89	6	4	7588	4	26	15
E-F	-53.8161	5.5854	-372.753	-420.9836	132	19	10	15047	6	11	17
E-G	-253.1919	-81.5164	-987.35	-1322.0582	198	51	42	39326	9	79	63
E-H	-35.5801	-46.1063	-161.938	-243.6244	87	7	3	7143	3	26	12
E-I	0.0	0.0	-0.0022	-0.0022	2	0	0	1	0	1	1
F-G	-27.1708	-50.3492	-177.392	-254.912	91	7	4	7767	4	25	15
F-H	0.0	0.0	-0.0148	-0.0148	2	0	0	15	0	0	0
F-J	0.0	0.0	-0.0024	-0.0024	2	0	0	1	0	1	1
G-H	-53.5079	5.688	-356.556	-404.3759	131	21	9	14632	7	11	18
G-I	0.0	0.0	-0.0086	-0.0086	2	0	0	13	0	0	0
G-J	0.0	0.0	-0.0024	-0.0024	2	0	0	1	0	1	1
H-I	-31.7401	-50.132	-163.024	-244.8961	89	10	3	7471	4	26	16
H-J	-17.2681	18.0328	-83.4477	-82.683	27	0	1	2789	8	6	6
I-J	-246.9294	-62.7954	-984.522	-1294.2467	197	50	43	39456	8	81	63

Interface residues

• A-B: ALA123B, ALA15A, ALA17A, ALA23A, ALA30A, ALA45A, ARG11A, ARG141A, ARG81B, ARG87A, ARG87B, ARG8B, ARG96A, ASN125B, ASN7B, ASN9A, ASN9B, ASP13A, ASP16A, ASP26A, ASP70B, ASP85A, ASP85B, ASP86A, ASP86B, ASP91A, CYS83A, CYS83B, GLN3B, GLN50A, GLN50B, GLN51B, GLN5B, GLN76B, GLU137A, GLU31A, GLU69B, GLU99A, GLY122B, GLY49A, GLY82B, ILE10B, ILE120B, ILE121B, ILE124B, ILE18A, ILE2B, ILE32A, ILE6B, ILE78B, ILE84A, ILE84B, ILE88A, LEU12A, LEU12B, LEU14A, LEU151B, LEU19A, LEU20A, LEU27A, LEU47A, LEU48A, LEU73B, LEU74B, LEU80B, LYS154B, LYS22A, LYS24A, LYS25A, MET1B, MET77B, MET94A, PHE156B, PHE29A, PRO155B, PRO79B, PRO89A, PRO92A, SER21A, SER28A, SER4B, SER71B, THR107A, THR153B, THR44A, THR90A, THR93A, TYR104A, TYR95A, VAL103A
• A-C: ARG87A, ARG87C
• A-D: ALA110A, ALA110D, ALA123A, ALA123D, ALA134A, ALA134D, ALA142A, ALA142D, ALA15A, ALA15D, ALA30A, ALA30D, ALA33A, ALA33D, ALA39A, ALA39D, ALA41A, ALA41D, ALA45A, ALA45D, ALA46A, ALA46D, ARG141A, ARG141D, ARG81A, ARG81D, ARG87A, ARG87D, ARG8A, ARG8D, ARG96A, ARG96D, ASN125A, ASN125D, ASP118A, ASP118D, ASP129A, ASP129D, ASP135A, ASP135D, ASP147A, ASP147D, ASP85A, ASP85D, ASP86A, ASP86D, ASP91A, ASP91D, CYS83A, CYS83D, GLN102A, GLN102D, GLN50A, GLN50D, GLN51A, GLN51D, GLU114A, GLU114D, GLU137A, GLU137D, GLU140A, GLU140D, GLU31A, GLU31D, GLU40A, GLU40D, GLU99A, GLU99D, GLY105A, GLY105D, GLY117A, GLY117D, GLY119A, GLY119D, GLY122A, GLY122D, GLY138A, GLY138D, GLY139A, GLY139D, GLY148A, GLY148D, GLY49A, GLY49D, GLY82A, GLY82D, HIS113A, HIS113D, ILE120A, ILE120D, ILE121A, ILE121D, ILE124A, ILE124D, ILE144A, ILE144D, ILE32A, ILE32D, ILE84A, ILE84D, ILE88A, ILE88D, LEU101A, LEU101D, LEU108A, LEU108D, LEU111A, LEU111D, LEU128A, LEU128D, LEU12A, LEU12D, LEU146A, LEU146D, LEU151A, LEU151D, LEU19A, LEU19D, LEU27A, LEU27D, LEU47A, LEU47D, LEU48A, LEU48D, LEU80A, LYS109A, LYS109D, LYS115A, LYS115D, LYS127A, LYS127D, LYS131A, LYS131D, LYS132A, LYS132D, LYS149A, LYS149D, LYS154D, LYS22A, LYS22D, MET100A, MET100D, MET94A, MET94D, PHE116A, PHE116D, PHE126A, PHE126D, PHE29A, PHE29D, PHE42A, PHE42D, PHE97A, PHE97D, PRO136A, PRO136D, PRO152A, PRO152D, PRO79A, PRO79D, PRO89A, PRO89D, PRO92A, PRO92D, SER28A, SER28D, THR106A, THR106D, THR107A, THR107D, THR145A, THR145D, THR153A, THR153D, THR44A, THR44D, THR93A, THR93D, TYR104A, TYR104D, TYR150A, TYR150D, TYR95A, TYR95D, TYR98A, TYR98D, VAL103A, VAL103D, VAL112A, VAL112D, VAL130A, VAL130D, VAL133A, VAL133D, VAL143A, VAL143D, VAL43A, VAL43D
• A-E: ARG87E, ASP86A
• A-F: ARG96A, ARG96F, ASP91A, ASP91F, GLN102A, GLN102F, GLU99A, GLU99F, ILE88A, ILE88F, LEU101F, MET100A, MET100F, PRO89A, PRO89F, PRO92A, PRO92F, THR90A, THR90F, THR93A, THR93F, TYR95A, TYR95F, TYR98A, TYR98F, VAL103A, VAL103F
• A-H: ARG87A, ASP86H
• A-I: ALA123A, ALA15I, ALA17I, ALA23I, ALA30I, ALA45I, ARG11I, ARG141I, ARG81A, ARG87A, ARG87I, ARG8A, ARG96I, ASN125A, ASN7A, ASN9A, ASN9I, ASP13I, ASP16I, ASP26I, ASP70A, ASP85A, ASP85I, ASP86A, ASP86I, ASP91I, CYS83A, CYS83I, GLN3A, GLN50A, GLN50I, GLN51A, GLN5A, GLN76A, GLU137I, GLU31I, GLU69A, GLU99I, GLY122A, GLY49I, GLY82A, ILE10A, ILE120A, ILE121A, ILE124A, ILE18I, ILE2A, ILE32I, ILE6A, ILE78A, ILE84A, ILE84I, ILE88I, LEU12I, LEU14I, LEU151A, LEU19I, LEU20I, LEU27I, LEU47I, LEU48I, LEU73A, LEU74A, LEU80A, LYS154A, LYS22I, LYS24I, LYS25I, MET1A, MET77A, MET94I, PHE156A, PHE29I, PRO155A, PRO79A, PRO89I, PRO92I, SER21I, SER28I, SER4A, SER71A, THR107I, THR153A, THR44I, THR90I, THR93I, TYR104I, TYR95I, VAL103I
• A-J: ALA110A, ALA110J, ALA123A, ALA123J, ALA39A, ALA39J, ALA41A, ALA41J, ALA45A, ALA45J, ALA46A, ALA46J, ALA52A, ALA52J, ALA57A, ALA57J, ARG81A, ARG81J, ARG87A, ARG87J, ARG8A, ARG8J, ASN125A, ASN125J, ASN7A, ASN7J, ASP118A, ASP118J, ASP129A, ASP129J, GLN3A, GLN3J, GLN50A, GLN50J, GLN51A, GLN51J, GLN5A, GLN5J, GLN76J, GLU114A, GLU114J, GLU40A, GLU40J, GLY105A, GLY105J, GLY117A, GLY117J, GLY119A, GLY119J, GLY122A, GLY122J, GLY37A, GLY37J, HIS113A, HIS113J, ILE120A, ILE120J, ILE121A, ILE121J, ILE124A, ILE124J, ILE2A, ILE2J, ILE6A, ILE6J, ILE78A, ILE78J, ILE88A, ILE88J, LEU108J, LEU111A, LEU111J, LEU128A, LEU128J, LEU151A, LEU151J, LEU38A, LEU38J, LEU53A, LEU53J, LEU80A, LEU80J, LYS109A, LYS109J, LYS115A, LYS115J, LYS127A, LYS127J, LYS149A, LYS149J, LYS154A, LYS154J, MET1A, MET1J, MET77A, MET77J, PHE116A, PHE116J, PHE126A, PHE126J, PHE156A, PHE156J, PHE42A, PHE42J, PRO152A, PRO152J, PRO155A, PRO155J, PRO54A, PRO54J, PRO79A, PRO79J, SER4A, SER4J, THR106A, THR106J, THR107A, THR107J, THR153A, THR153J, THR36J, TYR150A, TYR150J, TYR98A, TYR98J, VAL112A, VAL112J, VAL130A, VAL130J, VAL43A, VAL43J
• B-D: ALA110B, ALA110D, ALA123B, ALA123D, ALA39B, ALA39D, ALA41B, ALA41D, ALA45B, ALA45D, ALA46B, ALA46D, ALA52B, ALA52D, ALA57B, ALA57D, ARG81B, ARG81D, ARG87B, ARG87D, ARG8B, ARG8D, ASN125B, ASN125D, ASN7B, ASN7D, ASP118B, ASP118D, ASP129B, ASP129D, GLN3B, GLN3D, GLN50B, GLN50D, GLN51B, GLN51D, GLN5B, GLN5D, GLU114B, GLU114D, GLU40B, GLU40D, GLY105B, GLY105D, GLY117B, GLY117D, GLY119B, GLY119D, GLY122B, GLY122D, GLY37B, GLY37D, HIS113B, HIS113D, ILE120B, ILE120D, ILE121B, ILE121D, ILE124B, ILE124D, ILE2B, ILE2D, ILE6B, ILE6D, ILE78B, ILE78D, ILE88B, ILE88D, LEU111B, LEU111D, LEU128B, LEU128D, LEU151B, LEU151D, LEU38B, LEU38D, LEU53B, LEU53D, LEU80B, LEU80D, LYS109B, LYS109D, LYS115B, LYS115D, LYS127B, LYS127D, LYS149B, LYS149D, LYS154B, LYS154D, MET1B, MET1D, MET77B, MET77D, PHE116B, PHE116D, PHE126B, PHE126D, PHE156B, PHE156D, PHE42B, PHE42D, PRO152B, PRO152D, PRO155B, PRO155D, PRO54B, PRO54D, PRO79B, PRO79D, SER4B, SER4D, THR106B, THR106D, THR107B, THR107D, THR153B, THR153D, THR36B, THR36D, TYR150B, TYR150D, TYR98B, TYR98D, VAL112B, VAL112D, VAL130D, VAL43B, VAL43D
• B-E: ALA123E, ALA15B, ALA17B, ALA23B, ALA30B, ALA45B, ARG11B, ARG141B, ARG81E, ARG87B, ARG87E, ARG8E, ARG96B, ASN125E, ASN7E, ASN9B, ASN9E, ASP13B, ASP16B, ASP26B, ASP70E, ASP85B, ASP85E, ASP86B, ASP86E, ASP91B, CYS83B, CYS83E, GLN3E, GLN50B, GLN50E, GLN51E, GLN5E, GLN76E, GLU137B, GLU31B, GLU69E, GLU99B, GLY122E, GLY49B, GLY82E, ILE10E, ILE120E, ILE121E, ILE124E, ILE18B, ILE2E, ILE32B, ILE6E, ILE78E, ILE84B, ILE84E, ILE88B, LEU12B, LEU12E, LEU14B, LEU151E, LEU19B, LEU20B, LEU27B, LEU47B, LEU48B, LEU73E, LEU74E, LEU80E, LYS154E, LYS22B, LYS24B, LYS25B, MET1E, MET77E, MET94B, PHE156E, PHE29B, PRO155E, PRO79E, PRO89B, PRO92B, SER21B, SER28B, SER4E, SER71E, THR107B, THR153E, THR44B, THR90B, THR93B, TYR104B, TYR95B, TYR98B, VAL103B
• B-F: ALA110B, ALA110F, ALA123B, ALA123F, ALA134B, ALA134F, ALA142B, ALA142F, ALA15B, ALA15F, ALA30B, ALA30F, ALA33B, ALA33F, ALA39B, ALA39F, ALA41B, ALA41F, ALA45B, ALA45F, ALA46B, ALA46F, ARG141B, ARG141F, ARG81B, ARG81F, ARG87B, ARG87F, ARG8B, ARG8F, ARG96B, ARG96F, ASN125B, ASN125F, ASP118B, ASP118F, ASP129B, ASP129F, ASP135B, ASP135F, ASP147B, ASP147F, ASP85B, ASP85F, ASP86B, ASP86F, ASP91B, ASP91F, CYS83B, CYS83F, GLN102B, GLN102F, GLN50B, GLN50F, GLN51B, GLN51F, GLU114B, GLU114F, GLU137B, GLU137F, GLU140B, GLU140F, GLU31B, GLU31F, GLU40B, GLU40F, GLU99B, GLU99F, GLY105B, GLY105F, GLY117B, GLY117F, GLY119B, GLY119F, GLY122B, GLY122F, GLY138B, GLY138F, GLY139B, GLY139F, GLY148B, GLY148F, GLY49B, GLY49F, GLY82B, GLY82F, HIS113B, HIS113F, ILE120B, ILE120F, ILE121B, ILE121F, ILE124B, ILE124F, ILE144B, ILE144F, ILE32B, ILE32F, ILE84B, ILE84F, ILE88B, ILE88F, LEU101B, LEU101F, LEU108B, LEU108F, LEU111B, LEU111F, LEU128B, LEU128F, LEU12B, LEU12F, LEU146B, LEU146F, LEU151B, LEU151F, LEU19B, LEU19F, LEU27B, LEU27F, LEU47B, LEU47F, LEU48B, LEU48F, LEU80B, LEU80F, LYS109B, LYS109F, LYS115B, LYS115F, LYS127B, LYS127F, LYS131B, LYS131F, LYS132B, LYS132F, LYS149B, LYS149F, LYS154F, LYS22B, LYS22F, MET100B, MET100F, MET94B, MET94F, PHE116B, PHE116F, PHE126B, PHE126F, PHE29B, PHE29F, PHE42B, PHE42F, PHE97B, PHE97F, PRO136B, PRO136F, PRO152B, PRO152F, PRO79B, PRO79F, PRO89B, PRO89F, PRO92B, PRO92F, SER28B, SER28F, THR106B, THR106F, THR107B, THR107F, THR145B, THR145F, THR153B, THR153F, THR44B, THR44F, THR93B, THR93F, TYR104B, TYR104F, TYR150B, TYR150F, TYR95B, TYR95F, TYR98B, TYR98F, VAL103B, VAL103F, VAL112B, VAL112F, VAL130B, VAL130F, VAL133B, VAL133F, VAL143B, VAL143F, VAL43B, VAL43F
• B-G: ARG96B, ARG96G, ASP91B, ASP91G, GLN102B, GLN102G, GLU99B, GLU99G, ILE88B, ILE88G, MET100B, MET100G, PRO89B, PRO89G, PRO92B, PRO92G, THR90B, THR90G, THR93B, THR93G, TYR95B, TYR95G, TYR98B, TYR98G, VAL103B, VAL103G
• B-H: ARG87H, ASP86B
• B-I: ARG87B, ASP86I
• B-J: ARG87B, ARG87J
• C-D: ARG87C, ASP86D
• C-E: ARG96C, ARG96E, ASP91C, ASP91E, GLN102C, GLN102E, GLU99C, GLU99E, ILE88C, ILE88E, LEU101E, MET100C, MET100E, PRO89C, PRO89E, PRO92C, PRO92E, THR90C, THR90E, THR93C, THR93E, TYR95C, TYR95E, TYR98C, TYR98E, VAL103C, VAL103E
• C-F: ARG87F, ASP86C
• C-G: ALA123G, ALA15C, ALA17C, ALA23C, ALA30C, ALA45C, ARG11C, ARG141C, ARG81G, ARG87C, ARG87G, ARG8G, ARG96C, ASN125G, ASN7G, ASN9C, ASN9G, ASP13C, ASP16C, ASP26C, ASP70G, ASP85C, ASP85G, ASP86C, ASP86G, ASP91C, CYS83C, CYS83G, GLN3G, GLN50C, GLN50G, GLN51G, GLN5G, GLN76G, GLU137C, GLU31C, GLU69G, GLU99C, GLY122G, GLY49C, GLY82G, ILE10G, ILE120G, ILE121G, ILE124G, ILE18C, ILE2G, ILE32C, ILE6G, ILE78G, ILE84C, ILE84G, ILE88C, LEU12C, LEU14C, LEU151G, LEU19C, LEU20C, LEU27C, LEU47C, LEU48C, LEU73G, LEU74G, LEU80G, LYS154G, LYS22C, LYS24C, LYS25C, MET1G, MET77G, MET94C, PHE156G, PHE29C, PRO155G, PRO79G, PRO89C, PRO92C, SER21C, SER28C, SER4G, SER71G, THR107C, THR153G, THR44C, THR90C, THR93C, TYR104C, TYR95C, TYR98C, VAL103C
• C-H: ALA110C, ALA110H, ALA123C, ALA123H, ALA134C, ALA134H, ALA142C, ALA142H, ALA15C, ALA15H, ALA30C, ALA30H, ALA33C, ALA33H, ALA39C, ALA39H, ALA41C, ALA41H, ALA45C, ALA45H, ALA46C, ALA46H, ARG141C, ARG141H, ARG81C, ARG81H, ARG87C, ARG87H, ARG8C, ARG8H, ARG96C, ARG96H, ASN125C, ASN125H, ASP118C, ASP118H, ASP129C, ASP129H, ASP135C, ASP135H, ASP147C, ASP147H, ASP85C, ASP85H, ASP86C, ASP86H, ASP91C, ASP91H, CYS83C, CYS83H, GLN102C, GLN102H, GLN50C, GLN50H, GLN51C, GLN51H, GLU114C, GLU114H, GLU137C, GLU137H, GLU140C, GLU140H, GLU31C, GLU31H, GLU40C, GLU40H, GLU99C, GLU99H, GLY105C, GLY105H, GLY117C, GLY117H, GLY119C, GLY119H, GLY122C, GLY122H, GLY138C, GLY138H, GLY139C, GLY139H, GLY148C, GLY148H, GLY49C, GLY49H, GLY82C, GLY82H, HIS113C, HIS113H, ILE120C, ILE120H, ILE121C, ILE121H, ILE124C, ILE124H, ILE144C, ILE144H, ILE32C, ILE32H, ILE84C, ILE84H, ILE88C, ILE88H, LEU101C, LEU101H, LEU108C, LEU108H, LEU111C, LEU111H, LEU128C, LEU128H, LEU12C, LEU12H, LEU146C, LEU146H, LEU151C, LEU151H, LEU19C, LEU19H, LEU27C, LEU27H, LEU47C, LEU47H, LEU48C, LEU48H, LEU80H, LYS109C, LYS109H, LYS115C, LYS115H, LYS127C, LYS127H, LYS131C, LYS131H, LYS132C, LYS132H, LYS149C, LYS149H, LYS22C, LYS22H, MET100C, MET100H, MET94C, MET94H, PHE116C, PHE116H, PHE126C, PHE126H, PHE29C, PHE29H, PHE42C, PHE42H, PHE97C, PHE97H, PRO136C, PRO136H, PRO152C, PRO152H, PRO79C, PRO79H, PRO89C, PRO89H, PRO92C, PRO92H, SER28C, SER28H, THR106C, THR106H, THR107C, THR107H, THR145C, THR145H, THR153C, THR153H, THR44C, THR44H, THR93C, THR93H, TYR104C, TYR104H, TYR150C, TYR150H, TYR95C, TYR95H, TYR98C, TYR98H, VAL103C, VAL103H, VAL112C, VAL112H, VAL130C, VAL130H, VAL133C, VAL133H, VAL143C, VAL143H, VAL43C, VAL43H
• C-I: ALA110C, ALA110I, ALA123C, ALA123I, ALA39C, ALA39I, ALA41C, ALA41I, ALA45C, ALA45I, ALA46C, ALA46I, ALA52C, ALA52I, ALA57C, ALA57I, ARG81C, ARG81I, ARG87C, ARG87I, ARG8C, ARG8I, ASN125C, ASN125I, ASN7C, ASN7I, ASP118C, ASP118I, ASP129C, ASP129I, GLN3C, GLN3I, GLN50C, GLN50I, GLN51C, GLN51I, GLN5C, GLN5I, GLN76I, GLU114C, GLU114I, GLU40C, GLU40I, GLY105C, GLY105I, GLY117C, GLY117I, GLY119C, GLY119I, GLY122C, GLY122I, GLY37C, GLY37I, HIS113C, HIS113I, ILE120C, ILE120I, ILE121C, ILE121I, ILE124C, ILE124I, ILE2C, ILE2I, ILE6C, ILE6I, ILE78C, ILE78I, ILE88C, ILE88I, LEU108C, LEU108I, LEU111C, LEU111I, LEU128C, LEU128I, LEU151C, LEU151I, LEU38C, LEU38I, LEU53C, LEU53I, LEU80C, LEU80I, LYS109C, LYS109I, LYS115C, LYS115I, LYS127C, LYS127I, LYS149C, LYS149I, LYS154C, LYS154I, MET1C, MET1I, MET77C, MET77I, PHE116C, PHE116I, PHE126C, PHE126I, PHE156C, PHE156I, PHE42C, PHE42I, PRO152C, PRO152I, PRO155C, PRO155I, PRO54C, PRO54I, PRO79C, PRO79I, SER4C, SER4I, THR106C, THR106I, THR107C, THR107I, THR153C, THR153I, THR36C, THR36I, TYR150C, TYR150I, TYR98C, TYR98I, VAL112C, VAL112I, VAL130I, VAL43C, VAL43I
• C-J: ALA123C, ALA15J, ALA17J, ALA23J, ALA30J, ALA45J, ARG11J, ARG81C, ARG87C, ARG87J, ARG8C, ARG96J, ASN125C, ASN7C, ASN9C, ASN9J, ASP13J, ASP16J, ASP26J, ASP70C, ASP85C, ASP85J, ASP86C, ASP86J, ASP91J, CYS83C, CYS83J, GLN3C, GLN50C, GLN50J, GLN51C, GLN5C, GLN76C, GLU137J, GLU31J, GLU69C, GLU99J, GLY122C, GLY49J, GLY82C, ILE10C, ILE120C, ILE121C, ILE124C, ILE18J, ILE2C, ILE32J, ILE6C, ILE78C, ILE84C, ILE84J, ILE88J, LEU12J, LEU14J, LEU151C, LEU19J, LEU20J, LEU27J, LEU47J, LEU48J, LEU73C, LEU74C, LEU80C, LYS154C, LYS22J, LYS24J, LYS25J, MET1C, MET77C, MET94J, PHE156C, PHE29J, PRO155C, PRO79C, PRO89J, PRO92J, SER21J, SER28J, SER4C, SER71C, THR107J, THR153C, THR44J, THR90J, THR93J, TYR104J, TYR95J, TYR98J, VAL103J
• D-E: ARG87D, ARG87E
• D-F: ALA123D, ALA15F, ALA17F, ALA23F, ALA30F, ALA45F, ARG11F, ARG81D, ARG87D, ARG87F, ARG8D, ARG96F, ASN125D, ASN7D, ASN9D, ASN9F, ASP13F, ASP16F, ASP26F, ASP70D, ASP85D, ASP85F, ASP86D, ASP86F, ASP91F, CYS83D, CYS83F, GLN3D, GLN50D, GLN50F, GLN51D, GLN5D, GLN76D, GLU137F, GLU31F, GLU69D, GLU99F, GLY122D, GLY49F, GLY82D, ILE10D, ILE120D, ILE121D, ILE124D, ILE18F, ILE2D, ILE32F, ILE6D, ILE78D, ILE84D, ILE84F, ILE88F, LEU12D, LEU12F, LEU14F, LEU151D, LEU19F, LEU20F, LEU27F, LEU47F, LEU48F, LEU73D, LEU74D, LEU80D, LYS154D, LYS22F, LYS24F, LYS25F, MET1D, MET77D, MET94F, PHE156D, PHE29F, PRO155D, PRO79D, PRO89F, PRO92F, SER21F, SER28F, SER4D, SER71D, THR107F, THR153D, THR44F, THR90F, THR93F, TYR104F, TYR95F, TYR98F, VAL103F
• D-G: ARG87D, ASP86G
• D-I: ARG96D, ARG96I, ASP91D, ASP91I, GLN102D, GLN102I, GLU99D, GLU99I, ILE88D, ILE88I, LEU101I, MET100D, MET100I, PRO89D, PRO89I, PRO92D, PRO92I, THR90D, THR90I, THR93D, THR93I, TYR95D, TYR95I, TYR98D, TYR98I, VAL103D, VAL103I
• D-J: ALA123J, ALA15D, ALA17D, ALA23D, ALA30D, ALA45D, ARG11D, ARG81J, ARG87D, ARG87J, ARG8J, ARG96D, ASN125J, ASN7J, ASN9D, ASN9J, ASP13D, ASP16D, ASP26D, ASP70J, ASP85D, ASP85J, ASP86D, ASP86J, ASP91D, CYS83D, CYS83J, GLN3J, GLN50D, GLN50J, GLN51J, GLN5J, GLN76J, GLU137D, GLU31D, GLU69J, GLU99D, GLY122J, GLY49D, GLY82J, ILE10J, ILE120J, ILE121J, ILE124J, ILE18D, ILE2J, ILE32D, ILE6J, ILE78J, ILE84D, ILE84J, ILE88D, LEU12D, LEU14D, LEU151J, LEU19D, LEU20D, LEU27D, LEU47D, LEU48D, LEU73J, LEU74J, LEU80J, LYS154J, LYS22D, LYS24D, LYS25D, MET1J, MET77J, MET94D, PHE156J, PHE29D, PRO155J, PRO79J, PRO89D, PRO92D, SER21D, SER28D, SER4J, SER71J, THR107D, THR153J, THR44D, THR90D, THR93D, TYR104D, TYR95D, TYR98D, VAL103D
• E-F: ALA110E, ALA110F, ALA123E, ALA123F, ALA39E, ALA39F, ALA41E, ALA41F, ALA45E, ALA45F, ALA46E, ALA46F, ALA52E, ALA52F, ALA57E, ALA57F, ARG81E, ARG81F, ARG87E, ARG87F, ARG8E, ARG8F, ASN125E, ASN125F, ASN7E, ASN7F, ASP118E, ASP118F, ASP129E, ASP129F, GLN3E, GLN3F, GLN50E, GLN50F, GLN51E, GLN51F, GLN5E, GLN5F, GLN76E, GLN76F, GLU114E, GLU114F, GLU40E, GLU40F, GLY105E, GLY105F, GLY117E, GLY117F, GLY119E, GLY119F, GLY122E, GLY122F, GLY37E, GLY37F, HIS113E, HIS113F, ILE120E, ILE120F, ILE121E, ILE121F, ILE124E, ILE124F, ILE2E, ILE2F, ILE6E, ILE6F, ILE78E, ILE78F, ILE88E, ILE88F, LEU111E, LEU111F, LEU128E, LEU128F, LEU151E, LEU151F, LEU38E, LEU38F, LEU53E, LEU53F, LEU80E, LEU80F, LYS109E, LYS109F, LYS115E, LYS115F, LYS127E, LYS127F, LYS149E, LYS149F, LYS154E, LYS154F, MET1E, MET1F, MET77E, MET77F, PHE116E, PHE116F, PHE126E, PHE126F, PHE156E, PHE156F, PHE42E, PHE42F, PRO152E, PRO152F, PRO155E, PRO155F, PRO54E, PRO54F, PRO79E, PRO79F, SER4E, SER4F, THR106E, THR106F, THR107E, THR107F, THR153E, THR153F, THR36E, THR36F, TYR150E, TYR150F, TYR98E, TYR98F, VAL112E, VAL112F, VAL130E, VAL130F, VAL43E, VAL43F
• E-G: ALA110E, ALA110G, ALA123E, ALA123G, ALA134E, ALA134G, ALA142E, ALA142G, ALA15E, ALA15G, ALA30E, ALA30G, ALA33E, ALA33G, ALA39E, ALA39G, ALA41E, ALA41G, ALA45E, ALA45G, ALA46E, ALA46G, ARG141E, ARG141G, ARG81E, ARG81G, ARG87E, ARG87G, ARG8E, ARG8G, ARG96E, ARG96G, ASN125E, ASN125G, ASP118E, ASP118G, ASP129E, ASP129G, ASP135E, ASP135G, ASP147E, ASP147G, ASP85E, ASP85G, ASP86E, ASP86G, ASP91E, ASP91G, CYS83E, CYS83G, GLN102E, GLN102G, GLN50E, GLN50G, GLN51E, GLN51G, GLU114E, GLU114G, GLU137E, GLU137G, GLU140E, GLU140G, GLU31E, GLU31G, GLU40E, GLU40G, GLU99E, GLU99G, GLY105E, GLY105G, GLY117E, GLY117G, GLY119E, GLY119G, GLY122E, GLY122G, GLY138E, GLY138G, GLY139E, GLY139G, GLY148E, GLY148G, GLY49E, GLY49G, GLY82E, GLY82G, HIS113E, HIS113G, ILE120E, ILE120G, ILE121E, ILE121G, ILE124E, ILE124G, ILE144E, ILE144G, ILE32E, ILE32G, ILE84E, ILE84G, ILE88E, ILE88G, LEU101E, LEU101G, LEU108E, LEU108G, LEU111E, LEU111G, LEU128E, LEU128G, LEU12E, LEU12G, LEU146E, LEU146G, LEU151E, LEU151G, LEU19E, LEU19G, LEU27E, LEU27G, LEU47E, LEU47G, LEU48E, LEU48G, LEU80E, LEU80G, LYS109E, LYS109G, LYS115E, LYS115G, LYS127E, LYS127G, LYS131E, LYS131G, LYS132E, LYS132G, LYS149E, LYS149G, LYS22E, LYS22G, MET100E, MET100G, MET94E, MET94G, PHE116E, PHE116G, PHE126E, PHE126G, PHE29E, PHE29G, PHE42E, PHE42G, PHE97E, PHE97G, PRO136E, PRO136G, PRO152E, PRO152G, PRO79E, PRO79G, PRO89E, PRO89G, PRO92E, PRO92G, SER28E, SER28G, THR106E, THR106G, THR107E, THR107G, THR145E, THR145G, THR153E, THR153G, THR44E, THR44G, THR93E, THR93G, TYR104E, TYR104G, TYR150E, TYR150G, TYR95E, TYR95G, TYR98E, TYR98G, VAL103E, VAL103G, VAL112E, VAL112G, VAL130E, VAL130G, VAL133E, VAL133G, VAL143E, VAL143G, VAL43E, VAL43G
• E-H: ALA123H, ALA15E, ALA17E, ALA23E, ALA30E, ARG11E, ARG81H, ARG87E, ARG87H, ARG8H, ARG96E, ASN125H, ASN7H, ASN9E, ASN9H, ASP13E, ASP16E, ASP26E, ASP70H, ASP85E, ASP85H, ASP86E, ASP86H, ASP91E, CYS83E, CYS83H, GLN3H, GLN50E, GLN50H, GLN51H, GLN5H, GLN76H, GLU31E, GLU69H, GLU99E, GLY122H, GLY49E, GLY82H, ILE10H, ILE120H, ILE121H, ILE124H, ILE18E, ILE2H, ILE32E, ILE6H, ILE78H, ILE84E, ILE84H, ILE88E, LEU12E, LEU14E, LEU151H, LEU19E, LEU20E, LEU27E, LEU47E, LEU48E, LEU73H, LEU74H, LEU80H, LYS154H, LYS22E, LYS24E, LYS25E, MET1H, MET77H, MET94E, PHE156H, PHE29E, PRO155H, PRO79H, PRO89E, PRO92E, SER21E, SER28E, SER4H, SER71H, THR107E, THR153H, THR44E, THR90E, THR93E, TYR104E, TYR95E, TYR98E, VAL103E
• E-I: ARG87I, ASP86E
• F-G: ALA123F, ALA15G, ALA17G, ALA23G, ALA30G, ALA45G, ARG11G, ARG141G, ARG81F, ARG87F, ARG87G, ARG8F, ARG96G, ASN125F, ASN7F, ASN9F, ASN9G, ASP13G, ASP16G, ASP26G, ASP70F, ASP85F, ASP85G, ASP86F, ASP86G, ASP91G, CYS83F, CYS83G, GLN3F, GLN50F, GLN50G, GLN51F, GLN5F, GLN76F, GLU137G, GLU31G, GLU40G, GLU69F, GLU99G, GLY122F, GLY49G, GLY82F, ILE10F, ILE120F, ILE121F, ILE124F, ILE18G, ILE2F, ILE32G, ILE6F, ILE78F, ILE84F, ILE84G, ILE88G, LEU12F, LEU12G, LEU14G, LEU151F, LEU19G, LEU20G, LEU27G, LEU47G, LEU48G, LEU73F, LEU74F, LEU80F, LYS154F, LYS22G, LYS24G, LYS25G, MET1F, MET77F, MET94G, PHE156F, PHE29G, PRO155F, PRO79F, PRO89G, PRO92G, SER21G, SER28G, SER4F, SER71F, THR107G, THR153F, THR44G, THR90G, THR93G, TYR104G, TYR95G, VAL103G
• F-H: ARG87F, ARG87H
• F-J: ARG87J, ASP86F
• G-H: ALA110G, ALA110H, ALA123G, ALA123H, ALA39G, ALA39H, ALA41G, ALA41H, ALA45G, ALA45H, ALA46G, ALA52G, ALA52H, ALA57G, ALA57H, ARG81G, ARG81H, ARG87G, ARG87H, ARG8G, ARG8H, ASN125G, ASN125H, ASN7G, ASN7H, ASP118G, ASP118H, ASP129G, ASP129H, GLN3G, GLN3H, GLN50G, GLN50H, GLN51G, GLN51H, GLN5G, GLN5H, GLN76H, GLU114G, GLU114H, GLU40G, GLU40H, GLY105G, GLY105H, GLY117G, GLY117H, GLY119G, GLY119H, GLY122G, GLY122H, GLY37G, GLY37H, HIS113G, HIS113H, ILE120G, ILE120H, ILE121G, ILE121H, ILE124G, ILE124H, ILE2G, ILE2H, ILE6G, ILE6H, ILE78G, ILE78H, ILE88G, ILE88H, LEU108G, LEU108H, LEU111G, LEU111H, LEU128G, LEU128H, LEU151G, LEU151H, LEU38G, LEU38H, LEU53G, LEU53H, LEU80G, LEU80H, LYS109G, LYS109H, LYS115G, LYS115H, LYS127G, LYS127H, LYS149G, LYS149H, LYS154G, LYS154H, MET1G, MET1H, MET77G, MET77H, PHE116G, PHE116H, PHE126G, PHE126H, PHE156G, PHE156H, PHE42G, PHE42H, PRO152G, PRO152H, PRO155G, PRO155H, PRO54G, PRO54H, PRO79G, PRO79H, SER4G, SER4H, THR106G, THR106H, THR107G, THR107H, THR153G, THR153H, THR36G, THR36H, TYR150G, TYR150H, TYR98G, TYR98H, VAL112G, VAL112H, VAL130H, VAL43G, VAL43H
• G-I: ARG87G, ARG87I
• G-J: ARG87G, ASP86J
• H-I: ALA123I, ALA15H, ALA17H, ALA23H, ALA30H, ALA45H, ARG11H, ARG81I, ARG87H, ARG87I, ARG8I, ARG96H, ASN125I, ASN7I, ASN9H, ASN9I, ASP13H, ASP16H, ASP26H, ASP70I, ASP85H, ASP85I, ASP86H, ASP86I, ASP91H, CYS83H, CYS83I, GLN3I, GLN50H, GLN50I, GLN51I, GLN5I, GLN76I, GLU137H, GLU31H, GLU69I, GLU99H, GLY122I, GLY49H, GLY82I, ILE10I, ILE120I, ILE121I, ILE124I, ILE18H, ILE2I, ILE32H, ILE6I, ILE78I, ILE84H, ILE84I, ILE88H, LEU12H, LEU14H, LEU151I, LEU19H, LEU20H, LEU27H, LEU47H, LEU48H, LEU73I, LEU74I, LEU80I, LYS154I, LYS22H, LYS24H, LYS25H, MET1I, MET77I, MET94H, PHE156I, PHE29H, PRO155I, PRO79I, PRO89H, PRO92H, SER21H, SER28H, SER4I, SER71I, THR107H, THR153I, THR44H, THR90H, THR93H, TYR104H, TYR95H, TYR98H, VAL103H
• H-J: ARG96H, ARG96J, ASP91H, ASP91J, GLN102H, GLN102J, GLU99H, GLU99J, ILE88H, ILE88J, LEU101H, MET100H, MET100J, PRO89H, PRO89J, PRO92H, PRO92J, THR90H, THR90J, THR93H, THR93J, TYR95H, TYR95J, TYR98H, TYR98J, VAL103H, VAL103J
• I-J: ALA110I, ALA110J, ALA123I, ALA123J, ALA134I, ALA134J, ALA142I, ALA142J, ALA15I, ALA15J, ALA30I, ALA30J, ALA33I, ALA33J, ALA39I, ALA39J, ALA41I, ALA41J, ALA45I, ALA45J, ALA46I, ALA46J, ARG141I, ARG141J, ARG81I, ARG81J, ARG87I, ARG87J, ARG8I, ARG8J, ARG96I, ARG96J, ASN125I, ASN125J, ASP118I, ASP118J, ASP129I, ASP129J, ASP135I, ASP135J, ASP147I, ASP147J, ASP85I, ASP85J, ASP86I, ASP86J, ASP91I, ASP91J, CYS83I, CYS83J, GLN102I, GLN102J, GLN50I, GLN50J, GLN51I, GLN51J, GLU114I, GLU114J, GLU137I, GLU137J, GLU140I, GLU140J, GLU31I, GLU31J, GLU40I, GLU40J, GLU99I, GLU99J, GLY105I, GLY105J, GLY117I, GLY117J, GLY119I, GLY119J, GLY122I, GLY122J, GLY138I, GLY138J, GLY139I, GLY139J, GLY148I, GLY148J, GLY49I, GLY49J, GLY82I, GLY82J, HIS113I, HIS113J, ILE120I, ILE120J, ILE121I, ILE121J, ILE124I, ILE124J, ILE144I, ILE144J, ILE32I, ILE32J, ILE84I, ILE84J, ILE88I, ILE88J, LEU101I, LEU101J, LEU108I, LEU108J, LEU111I, LEU111J, LEU128I, LEU128J, LEU12I, LEU12J, LEU146I, LEU146J, LEU151I, LEU151J, LEU19I, LEU19J, LEU27I, LEU27J, LEU47I, LEU47J, LEU48I, LEU48J, LEU80J, LYS109I, LYS109J, LYS115I, LYS115J, LYS127I, LYS127J, LYS131I, LYS131J, LYS132I, LYS132J, LYS149I, LYS149J, LYS22I, LYS22J, MET100I, MET100J, MET94I, MET94J, PHE116I, PHE116J, PHE126I, PHE126J, PHE29I, PHE29J, PHE42I, PHE42J, PHE97I, PHE97J, PRO136I, PRO136J, PRO152I, PRO152J, PRO79I, PRO79J, PRO89I, PRO89J, PRO92I, PRO92J, SER28I, SER28J, THR106I, THR106J, THR107I, THR107J, THR145I, THR145J, THR153I, THR153J, THR44I, THR44J, THR93I, THR93J, TYR104I, TYR104J, TYR150I, TYR150J, TYR95I, TYR95J, TYR98I, TYR98J, VAL103I, VAL103J, VAL112I, VAL112J, VAL130I, VAL130J, VAL133I, VAL133J, VAL143I, VAL143J, VAL43I, VAL43J