2I2U

crystal structure of ribosome with messenger rna and the anticodon stem-loop of p-site trna. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains two 70s ribosomes and is described in remark 400.

• Total interactions analyzed: 253
• Total true interactions: 22
• Strongest Interaction:
  • Chains: J-N
  • Int. Res.: 51
  • Norm. En. per Res.: -3.866
• Hub Node: C(4)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
B-E	0.0	0.0	-0.4845	-0.4845	7	0	0	115	0	1	4
C-D	0.0	0.0	-0.1328	-0.1328	3	0	0	34	0	0	4
C-E	0.0	0.0	-4.4942	-4.4942	14	0	0	334	0	8	7
C-J	0.0	0.0	-52.1423	-52.1423	55	4	3	2464	0	13	8
C-N	-9.5905	-29.1695	-229.118	-267.878	76	17	4	9765	0	33	32
D-E	0.0	0.0	-1.5924	-1.5924	18	0	0	163	0	4	2
E-H	-17.8456	-3.7346	-198.299	-219.8792	73	26	2	8204	0	17	15
F-R	-18.2024	-0.5434	-145.517	-164.2627	59	11	3	6097	0	11	26
G-I	0.0	0.0	-5.2668	-5.2668	20	0	0	532	0	10	6
G-K	0.0	1.1276	-27.7035	-26.5759	22	9	2	1750	0	1	4
H-L	0.0	0.0	-2.5229	-2.5229	8	0	1	285	0	0	0
H-Q	0.0	2.3817	-11.6248	-9.2431	15	5	0	1156	0	3	7
I-J	0.0	0.0	-9.0789	-9.0789	17	0	0	838	0	8	9
I-N	0.0	0.0	-20.8469	-20.8469	7	0	0	592	0	0	1
J-N	-5.5023	-16.6083	-175.057	-197.1676	51	21	5	7270	0	15	25
K-R	-5.1066	-10.4917	-17.6879	-33.2862	21	3	0	881	0	6	9
K-U	-3.2772	73.4033	-217.724	-147.5979	76	40	5	10484	1	31	35
L-Q	0.0	0.0	-41.5595	-41.5595	23	5	4	2095	0	0	8
M-S	-4.1336	7.8453	-60.6821	-56.9705	42	7	0	3268	0	10	22
N-S	0.0	2.4392	-39.0894	-36.6502	31	8	0	2371	0	5	12
O-Q	0.0	0.0	-0.4995	-0.4995	3	0	0	80	0	0	0
R-U	0.0	-0.7632	-42.3079	-43.0711	22	13	0	2063	0	12	12

Interface residues

• B-E: ARG68E, ASN177B, GLU64E, LYS104B, LYS65E, THR101B, TRP103B
• C-D: ARG131C, ARG46D, LYS44D
• C-E: ARG130C, ARG163C, ARG168C, ARG19E, ARG53E, GLU169C, GLU54E, LYS134C, PHE30E, PHE32E, PRO56E, TRP166C, TYR167C, VAL55E
• C-J: ALA12J, ALA23C, ALA60C, ALA93J, ALA94J, ARG16J, ARG45J, ARG58C, ARG64C, ARG68J, ARG9J, ASN24C, ASP14J, ASP63J, ASP97J, GLN2C, GLN99C, GLN99J, GLU47J, GLU57C, GLU66J, GLY12C, GLY1C, GLY8C, GLY95J, HIS5C, HIS70J, ILE13C, ILE53J, ILE67J, ILE9C, LEU11C, LEU17J, LEU32C, LEU71J, LEU92J, LYS11J, LYS3C, LYS61C, PHE13J, PHE22C, PHE28C, PHE49J, PRO59C, SER19C, SER62C, THR20C, THR25C, THR69J, TRP17C, TRP21C, TYR65J, VAL4C, VAL96J, VAL98J
• C-N: ALA23C, ALA29C, ALA86N, ALA87N, ARG10C, ARG39C, ARG53C, ARG58C, ARG64N, ARG74N, ARG84N, ARG89N, ASN184C, ASN18C, ASN24C, ASN31C, ASN7C, ASP30C, ASP33C, ASP35C, GLN2C, GLN40C, GLN65N, GLU27C, GLU57C, GLU85N, GLU91N, GLY12C, GLY77N, GLY8C, GLY90N, GLY94N, HIS5C, ILE13C, ILE54C, ILE56C, ILE92N, ILE93C, ILE9C, LEU11C, LEU177C, LEU32C, LEU73N, LEU78N, LEU95N, LYS113C, LYS15C, LYS198C, LYS26C, LYS37C, LYS3C, LYS75N, LYS82N, LYS96N, LYS97N, MET88N, PHE22C, PHE28C, PHE36C, PHE72N, PHE76N, PRO16C, PRO6C, PRO93N, SER19C, SER34C, THR185C, THR20C, THR25C, THR66N, TRP17C, TRP21C, TYR183C, VAL4C, VAL55C, VAL83N
• D-E: ALA106E, ALA79D, ARG111E, ASN84D, ASP49D, GLU201D, GLY101E, GLY103E, GLY107E, GLY108E, GLY83D, GLY86D, HIS197D, ILE104E, LYS82D, THR102E, THR85D, VAL200D
• E-H: ALA129H, ALA153E, ALA154E, ALA22H, ALA69H, ALA74E, ALA96H, ALA98E, ARG156E, ASN145E, ASN147E, ASN72E, ASN76E, ASP47H, GLN66H, GLN75H, GLN81E, GLN96E, GLU144E, GLU150E, GLU41H, GLU42H, GLU46H, GLU72H, GLY157E, GLY43H, GLY67H, GLY97H, GLY99H, HIS82E, ILE45H, ILE71E, ILE74H, LEU123E, LEU143E, LEU39H, LEU62H, LEU80E, LEU98H, LYS155E, LYS158E, LYS40H, LYS63H, LYS68H, LYS85E, LYS93H, MET146E, MET151E, MET95E, MET95H, PHE44H, PHE48H, PHE65H, PHE94E, PRO149E, PRO83E, PRO97E, SER148E, SER73H, SER99E, THR61H, THR79E, TYR127H, TYR64H, VAL122E, VAL128H, VAL152E, VAL38H, VAL70H, VAL71H, VAL73E, VAL84E, VAL94H
• F-R: ALA26R, ALA48F, ALA57F, ALA57R, ALA61R, ALA99F, ARG38F, ARG45F, ARG56R, ARG60R, ARG62R, ARG72R, ARG86F, ARG91F, ASN63F, ASP21R, ASP24R, GLN46F, GLN53R, GLU40F, GLU5F, GLU98F, HIS55F, HIS73R, ILE20R, ILE25R, ILE51F, ILE6F, ILE85F, LEU47F, LEU61F, LEU64R, LEU66R, LEU67R, LYS23R, LYS29R, LYS56F, LYS59R, LYS93F, MET88F, MET90F, MET9F, PHE8F, PRO50F, PRO68R, SER100F, SER65R, SER87F, THR70R, TRP42F, TYR22R, TYR49F, TYR59F, TYR63R, TYR69R, VAL60F, VAL7F, VAL89F, VAL96F
• G-I: ALA100I, ARG108I, ARG40I, ARG44I, ARG79I, ARG9G, GLN49I, GLU39G, GLU41I, ILE71I, LEU12G, LYS16G, LYS35G, MET45I, PHE102I, PRO15G, SER36G, THR42I, TYR43G, VAL46I
• G-K: ALA144G, ALA146G, ALA149G, ALA151G, ALA61K, ARG55K, ASN147G, GLU145G, LYS148G, LYS56K, MET143G, PHE150G, PHE51K, PHE60K, PRO59K, PRO87G, SER54K, SER57K, THR58K, TYR84G, VAL64K, VAL86G
• H-L: ARG79H, ASN4L, GLY81H, LEU82H, PRO80H, THR2L, VAL3L, VAL7L
• H-Q: ARG79H, ARG83H, GLY34Q, GLY81H, ILE37Q, ILE84H, LEU82H, LYS29Q, LYS35Q, LYS38Q, PHE27Q, PHE36Q, PRO80H, TYR33Q, TYR85H
• I-J: ALA61J, ARG112I, ARG123I, ARG48J, ARG62J, ASP60J, ASP63J, GLN64J, GLY116I, LEU117I, LEU52J, LYS113I, LYS114I, LYS59J, PRO124I, THR50J, VAL115I
• I-N: ALA98N, ARG112I, GLU111I, LYS113I, LYS114I, SER99N, TRP100N
• J-N: ALA61J, ALA86N, ALA87N, ALA98N, ARG48J, ARG62J, ARG68J, ARG68N, ARG80N, ARG84N, ASN58J, ASP14J, ASP63J, GLN64J, GLU47J, GLU66J, GLU85N, GLY71N, GLY94N, HIS56J, ILE53J, ILE67J, ILE81N, ILE92N, LEU52J, LEU73N, LEU78N, LEU95N, LYS46J, LYS59J, LYS75N, LYS82N, LYS96N, LYS97N, MET88N, PHE13J, PHE49J, PHE72N, PHE76N, PRO55J, PRO93N, SER54J, SER79N, SER99N, THR50J, THR69J, TRP100N, TYR65J, VAL51J, VAL57J, VAL83N
• K-R: ARG62R, ARG72R, ASN108K, ASP111K, ASP71R, GLY36R, HIS117K, ILE109K, ILE115K, LYS37R, LYS86K, MET84K, PRO114K, PRO68R, THR110K, THR113K, THR70R, TYR63R, TYR69R, VAL112K, VAL85K
• K-U: ALA14U, ALA24K, ALA25U, ALA29U, ALA98K, ARG105K, ARG121K, ARG126K, ARG127K, ARG16U, ARG17U, ARG20U, ARG32U, ARG33U, ARG34U, ARG6U, ARG92K, ARG97K, ASN100K, ASN108K, ASN118K, ASN27K, ASN8U, ASP111K, ASP12U, CYS120K, CYS22U, GLU23U, GLU30U, GLU35U, GLU7U, GLU82K, GLU93K, GLU9U, GLY119K, GLY26U, GLY87K, GLY89K, HIS23K, ILE106K, ILE109K, ILE115K, ILE3U, ILE96K, LEU15U, LEU28U, LYS124K, LYS125K, LYS19U, LYS24U, LYS4U, LYS86K, MET84K, PHE11U, PHE18U, PHE26K, PHE36U, PRO10U, PRO114K, PRO116K, PRO122K, PRO123K, PRO88K, SER21U, SER94K, THR107K, THR110K, THR113K, TYR37U, VAL112K, VAL13U, VAL27U, VAL31U, VAL5U, VAL83K, VAL85K
• L-Q: ALA1L, ARG11L, ARG13L, ARG39Q, ARG8L, ASN4L, GLN5L, GLY34Q, HIS30Q, ILE32Q, ILE37Q, LEU6L, LYS35Q, LYS38Q, LYS9L, PHE36Q, PRO10L, PRO31Q, THR2L, TYR33Q, VAL28Q, VAL3L, VAL7L
• M-S: ALA74S, ARG100M, ARG2S, ARG35S, ARG77S, ARG78M, ARG80S, ARG86M, ARG89M, ARG92M, ARG97M, ASP63S, ASP81M, CYS84M, GLN99M, GLU64S, GLU72S, GLY67S, GLY71S, GLY83M, GLY87M, GLY98M, HIS68S, LEU70S, LEU79M, LEU82M, LEU88M, LEU94M, LYS109M, LYS69S, MET65S, PHE73S, PRO75S, THR101M, THR62S, THR76S, TYR79S, TYR85M, VAL59S, VAL61S, VAL66S, VAL96M
• N-S: ALA43N, ARG36S, ARG40N, ARG52N, ASN42N, ASP11S, ASP32N, GLN48N, GLU19S, GLY7S, HIS13S, ILE10S, LEU12S, LEU14S, LEU15S, LEU45N, LEU47N, LEU4S, LEU50N, LYS46N, LYS5S, LYS6S, PHE40S, PHE9S, PRO8S, SER31N, SER34S, THR49N, TRP33S, TRP41N, VAL33N
• O-Q: LYS70O, VAL74O, VAL82Q
• R-U: ARG6U, ARG72R, ASP71R, CYS22U, GLU23U, GLU34R, GLY36R, ILE38R, ILE3U, LEU28U, LYS19U, LYS24U, LYS37R, LYS4U, PHE18U, PRO40R, SER21U, SER35R, THR33R, THR70R, VAL27U, VAL5U