2GX2

crystal structural and functional analysis of gfp-like fluorescent protein dronpa

• Total interactions analyzed: 66
• Total true interactions: 27
• Strongest Interaction:
  • Chains: F-G
  • Int. Res.: 109
  • Norm. En. per Res.: -3.903
• Hub Node: A(6)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
A-C	0.0	0.3115	-3.4228	-3.1113	16	0	0	291	1	14	15
A-E	-9.936	-14.2374	-62.9694	-87.1427	39	6	0	2764	0	12	15
A-H	-88.9615	-1.2277	-319.994	-410.1831	109	17	4	12094	13	75	77
A-J	0.0	-9.3841	-8.6612	-18.0452	12	0	0	385	2	16	16
A-K	0.0	4.2741	-0.3634	3.9106	6	0	0	44	0	4	4
A-L	-62.1719	2.3746	-213.388	-273.1853	102	10	1	8754	0	27	23
B-C	-86.1614	8.6672	-313.437	-390.9313	104	22	4	11906	13	67	71
B-F	0.0	4.2382	-0.3427	3.8956	6	0	0	44	0	4	4
B-G	-46.8914	-0.4309	-212.5	-259.8223	102	7	1	8853	0	28	23
C-F	-47.154	-1.0994	-219.158	-267.4114	104	8	1	8965	0	28	23
C-G	0.0	4.2796	-0.3545	3.9251	6	0	0	45	0	4	5
C-J	0.0	-3.1498	-12.8922	-16.042	19	0	0	746	0	7	6
D-E	-95.6293	-3.8328	-317.248	-416.7101	108	18	4	12082	13	77	78
D-I	-42.454	2.717	-216.83	-256.567	101	6	1	8828	0	26	23
D-J	0.0	4.3293	-0.4364	3.893	6	0	0	53	0	4	6
E-F	-17.1005	-24.5874	-58.3065	-99.9944	40	3	0	2733	3	17	18
E-I	0.0	4.2703	-0.3656	3.9046	6	0	0	45	0	4	4
E-J	-41.2232	-1.074	-215.943	-258.2402	103	11	1	8913	0	28	24
E-K	0.0	0.0	-0.0655	-0.0655	6	0	0	35	0	3	2
E-L	0.0	6.5104	-0.5762	5.9342	11	0	0	123	0	7	8
F-G	-99.6996	-8.3269	-317.396	-425.4225	109	15	4	12043	13	74	78
F-L	0.0	-11.9191	-1.5917	-13.5108	10	0	0	124	1	10	8
H-J	0.0	0.0	-0.0311	-0.0311	4	0	0	15	0	2	2
H-K	-41.0397	-0.7494	-219.434	-261.2232	103	11	1	8951	0	28	23
H-L	0.0	4.2515	-0.3517	3.8998	6	0	0	46	0	4	4
I-J	-87.8648	1.2798	-372.891	-459.476	120	23	4	14042	12	74	77
K-L	-63.4399	16.9755	-233.588	-280.0524	94	11	4	9220	10	62	64

Interface residues

• A-C: ASN206A, ASP110C, ASP201A, GLU26A, GLU85C, GLY111C, ILE115C, LEU109C, LYS181C, LYS202A, LYS43A, LYS45A, PRO84C, SER205A, VAL182C, VAL3C
• A-E: ALA127A, ALA24E, ARG135A, ARG13E, ASN128A, ASN206E, ASP201E, ASP203A, ASP203E, GLN133A, GLU15E, GLU164A, GLU46E, GLY129A, GLY165A, GLY47E, GLY48E, HIS21E, ILE25E, LEU163A, LEU50A, LEU50E, LYS134A, LYS202A, LYS202E, LYS43E, LYS45E, MET132A, PHE23E, PHE52A, PRO130A, PRO22E, PRO49A, PRO49E, PRO51A, PRO51E, SER205E, VAL131A, VAL44E
• A-H: ALA160A, ALA160H, ALA213A, ALA215A, ALA69A, ARG149A, ARG149H, ARG170A, ARG170H, ARG221H, ASN158A, ASN158H, ASN94A, ASN94H, ASP150A, ASP150H, ASP156A, ASP156H, ASP172A, ASP172H, ASP187A, ASP187H, ASP192A, ASP192H, ASP97A, ASP97H, CYS171A, CYS171H, GLU140A, GLU140H, GLU144A, GLU144H, GLU196H, GLU211A, GLU214A, GLU218A, GLU218H, GLU35A, GLU96A, GLU96H, GLY155A, GLY155H, GLY166A, GLY166H, GLY167A, GLY167H, GLY36A, HIS168A, HIS168H, HIS189A, HIS189H, HIS193A, HIS193H, HIS194A, HIS194H, HIS212A, HIS216A, HIS216H, ILE195A, LEU146A, LEU146H, LEU161A, LEU161H, LEU163A, LEU163H, LEU219H, LYS138H, LYS145A, LYS145H, LYS154A, LYS154H, LYS174A, LYS174H, LYS37A, LYS70A, MET159A, MET159H, PHE173A, PHE173H, PHE190A, PHE190H, PRO130H, PRO141A, PRO141H, PRO186A, PRO186H, PRO220H, SER142A, SER142H, SER162A, SER162H, SER217A, SER217H, THR143A, THR143H, TRP139A, TRP139H, TYR147A, TYR147H, TYR169A, TYR169H, TYR188A, TYR188H, VAL148A, VAL148H, VAL157A, VAL157H, VAL191A, VAL191H
• A-J: ALA24J, ARG119J, ARG13J, ASP110J, ASP201A, GLU117J, GLU15J, GLU46J, HIS200A, LYS198A, LYS202A, PHE23J
• A-K: ASP97A, ASP97K, GLU96A, GLU96K, GLY98A, GLY98K
• A-L: ALA103A, ALA103L, ALA127A, ALA127L, ALA17A, ALA17L, ARG119A, ARG119L, ARG149A, ARG149L, ARG91A, ARG91L, ASN102A, ASN102L, ASN105A, ASN105L, ASN124A, ASN124L, ASN128A, ASN128L, ASN19A, ASN19L, ASN94A, ASN94L, ASP106A, ASP106L, ASP121A, ASP121L, ASP150A, ASP150L, ASP156A, ASP156L, ASP97A, ASP97L, CYS101A, CYS101L, GLU117A, GLU117L, GLU15A, GLU15L, GLU90A, GLU90L, GLY122A, GLY122L, GLY129A, GLY129L, GLY155A, GLY155L, GLY16A, GLY16L, GLY20A, GLY20L, GLY98A, GLY98L, GLY99A, GLY99L, HIS21A, HIS21L, ILE100A, ILE100L, LEU153L, LYS154A, LYS154L, LYS174A, LYS174L, LYS178A, LYS178L, LYS180A, MET132A, MET132L, MET93A, MET93L, PHE120A, PHE120L, PHE125A, PHE125L, PHE173A, PHE173L, PRO126A, PRO126L, PRO22A, PRO22L, SER88A, SER88L, SER92A, SER92L, THR104A, THR104L, THR175A, THR175L, THR176A, THR176L, TRP89A, TRP89L, TYR177A, TYR177L, VAL123A, VAL123L, VAL152A, VAL152L, VAL18A, VAL18L
• B-C: ALA160B, ALA160C, ALA213B, ALA215B, ARG149B, ARG149C, ARG170B, ARG170C, ARG221C, ASN158B, ASN158C, ASN94B, ASN94C, ASP150B, ASP150C, ASP156B, ASP156C, ASP172B, ASP172C, ASP187B, ASP187C, ASP192B, ASP192C, CYS171B, CYS171C, GLU140B, GLU140C, GLU144B, GLU144C, GLU211B, GLU214B, GLU218C, GLU35B, GLU96B, GLU96C, GLY151C, GLY155B, GLY155C, GLY166B, GLY166C, GLY167B, GLY167C, GLY36B, HIS168B, HIS168C, HIS189B, HIS189C, HIS193B, HIS193C, HIS194B, HIS194C, HIS212B, HIS216B, HIS216C, ILE195B, LEU146B, LEU146C, LEU161B, LEU161C, LEU163B, LEU163C, LEU219C, LYS138C, LYS145B, LYS145C, LYS154B, LYS154C, LYS174B, LYS174C, LYS37B, LYS70B, MET159B, MET159C, PHE173B, PHE173C, PHE190B, PHE190C, PRO141B, PRO141C, PRO186B, PRO186C, PRO220C, SER142B, SER142C, SER162B, SER162C, SER217B, SER217C, THR143B, THR143C, TRP139B, TRP139C, TYR147B, TYR147C, TYR169B, TYR169C, TYR188B, TYR188C, VAL148B, VAL148C, VAL157B, VAL157C, VAL191B, VAL191C
• B-F: ASP97B, ASP97F, GLU96B, GLU96F, GLY98B, GLY98F
• B-G: ALA103B, ALA103G, ALA127B, ALA127G, ALA17B, ALA17G, ARG119B, ARG119G, ARG149B, ARG149G, ARG91B, ARG91G, ASN102B, ASN102G, ASN105B, ASN105G, ASN124B, ASN124G, ASN128B, ASN128G, ASN19B, ASN19G, ASN94B, ASN94G, ASP106B, ASP106G, ASP121B, ASP121G, ASP150B, ASP150G, ASP156B, ASP156G, ASP97B, ASP97G, CYS101B, CYS101G, GLU117B, GLU117G, GLU15B, GLU15G, GLU90B, GLU90G, GLY122B, GLY122G, GLY129B, GLY129G, GLY155B, GLY155G, GLY16B, GLY16G, GLY20B, GLY20G, GLY98B, GLY98G, GLY99B, GLY99G, HIS21B, HIS21G, ILE100B, ILE100G, LYS154B, LYS154G, LYS174B, LYS174G, LYS178B, LYS178G, LYS180B, LYS180G, MET132B, MET132G, MET93B, MET93G, PHE120B, PHE120G, PHE125B, PHE125G, PHE173B, PHE173G, PRO126B, PRO126G, PRO22B, PRO22G, SER88B, SER88G, SER92B, SER92G, THR104B, THR104G, THR175B, THR175G, THR176B, THR176G, TRP89B, TRP89G, TYR177B, TYR177G, VAL123B, VAL123G, VAL152B, VAL152G, VAL18B, VAL18G
• C-F: ALA103C, ALA103F, ALA127C, ALA127F, ALA17C, ALA17F, ARG119C, ARG119F, ARG149C, ARG149F, ARG91C, ARG91F, ASN102C, ASN102F, ASN105C, ASN105F, ASN124C, ASN124F, ASN128C, ASN128F, ASN19C, ASN19F, ASN94C, ASN94F, ASP106C, ASP106F, ASP121C, ASP121F, ASP150C, ASP150F, ASP156C, ASP156F, ASP97C, ASP97F, CYS101C, CYS101F, GLU117C, GLU117F, GLU15C, GLU15F, GLU90C, GLU90F, GLY122C, GLY122F, GLY129C, GLY129F, GLY151C, GLY155C, GLY155F, GLY16C, GLY16F, GLY20C, GLY20F, GLY98C, GLY98F, GLY99C, GLY99F, HIS21C, HIS21F, ILE100C, ILE100F, ILE118F, LYS154C, LYS154F, LYS174C, LYS174F, LYS178C, LYS178F, LYS180C, LYS180F, MET132C, MET132F, MET93C, MET93F, PHE120C, PHE120F, PHE125C, PHE125F, PHE173C, PHE173F, PRO126C, PRO126F, PRO22C, PRO22F, SER88C, SER88F, SER92C, SER92F, THR104C, THR104F, THR175C, THR175F, THR176C, THR176F, TRP89C, TRP89F, TYR177C, TYR177F, VAL123C, VAL123F, VAL152C, VAL152F, VAL18C, VAL18F
• C-G: ASP97C, ASP97G, GLU96C, GLU96G, GLY98C, GLY98G
• C-J: ASP203J, GLN184C, GLN81C, GLU46J, GLY47J, GLY48J, LEU185C, LEU50J, LYS181C, LYS45J, PHE23J, PRO186C, PRO22J, PRO49J, SER205J, VAL182C, VAL183C, VAL44J, VAL76C
• D-E: ALA160D, ALA160E, ALA213D, ALA215D, ALA69D, ARG149D, ARG149E, ARG170D, ARG170E, ARG221E, ASN158D, ASN158E, ASN94D, ASN94E, ASP150D, ASP150E, ASP156D, ASP156E, ASP172D, ASP172E, ASP187D, ASP187E, ASP192D, ASP192E, CYS171D, CYS171E, GLU140D, GLU140E, GLU144D, GLU144E, GLU196E, GLU211D, GLU214D, GLU218D, GLU218E, GLU35D, GLU96D, GLU96E, GLY155D, GLY155E, GLY166D, GLY166E, GLY167D, GLY167E, GLY36D, HIS168D, HIS168E, HIS189D, HIS189E, HIS193D, HIS193E, HIS194D, HIS194E, HIS212D, HIS216D, HIS216E, ILE195D, LEU146D, LEU146E, LEU161D, LEU161E, LEU163D, LEU163E, LEU219E, LYS138E, LYS145D, LYS145E, LYS154D, LYS154E, LYS174D, LYS174E, LYS37D, LYS70D, MET159D, MET159E, PHE173D, PHE173E, PHE190D, PHE190E, PRO130D, PRO130E, PRO141D, PRO141E, PRO186D, PRO186E, PRO220E, SER142D, SER142E, SER162D, SER162E, SER217D, SER217E, THR143D, THR143E, TRP139D, TRP139E, TYR147D, TYR147E, TYR169D, TYR169E, TYR188D, TYR188E, VAL148D, VAL148E, VAL157D, VAL157E, VAL191D, VAL191E
• D-I: ALA103D, ALA103I, ALA127D, ALA127I, ALA17D, ALA17I, ARG119D, ARG119I, ARG149D, ARG149I, ARG91D, ARG91I, ASN102D, ASN102I, ASN105D, ASN105I, ASN124D, ASN124I, ASN128D, ASN128I, ASN19D, ASN19I, ASN94D, ASN94I, ASP106D, ASP106I, ASP121D, ASP121I, ASP150D, ASP150I, ASP156D, ASP156I, ASP97D, ASP97I, CYS101D, CYS101I, GLU117D, GLU117I, GLU15D, GLU15I, GLU90D, GLU90I, GLY122D, GLY122I, GLY129D, GLY129I, GLY155D, GLY155I, GLY16D, GLY16I, GLY20D, GLY20I, GLY98D, GLY98I, GLY99D, GLY99I, HIS21D, HIS21I, ILE100D, ILE100I, LYS154D, LYS154I, LYS174D, LYS174I, LYS178D, LYS178I, LYS180D, MET132D, MET132I, MET93D, MET93I, PHE120D, PHE120I, PHE125D, PHE125I, PHE173D, PHE173I, PRO126D, PRO126I, PRO22D, PRO22I, SER88D, SER88I, SER92D, SER92I, THR104D, THR104I, THR175D, THR175I, THR176D, THR176I, TRP89D, TRP89I, TYR177D, TYR177I, VAL123D, VAL123I, VAL152D, VAL152I, VAL18D, VAL18I
• D-J: ASP97D, ASP97J, GLU96D, GLU96J, GLY98D, GLY98J
• E-F: ALA127F, ARG135F, ASN128F, ASP110E, ASP112E, ASP203F, CYS113E, GLN133F, GLU164F, GLU85E, GLY111E, GLY129F, GLY165F, GLY166F, GLY86E, ILE115E, ILE4E, LEU109E, LEU163F, LEU50F, LYS11E, LYS134F, LYS202F, LYS5E, LYS9E, MET132F, PHE52F, PHE83E, PRO130F, PRO49F, PRO51F, PRO84E, SER2E, THR108E, THR136F, TYR204F, VAL131F, VAL137F, VAL28E, VAL3E
• E-I: ASP97E, ASP97I, GLU96E, GLU96I, GLY98E, GLY98I
• E-J: ALA103E, ALA103J, ALA127E, ALA127J, ALA17E, ALA17J, ARG119E, ARG119J, ARG149E, ARG149J, ARG91E, ARG91J, ASN102E, ASN102J, ASN105E, ASN105J, ASN124E, ASN124J, ASN128E, ASN128J, ASN19E, ASN19J, ASN94E, ASN94J, ASP106E, ASP106J, ASP121E, ASP121J, ASP150E, ASP150J, ASP156E, ASP156J, ASP97E, ASP97J, CYS101E, CYS101J, GLU117E, GLU117J, GLU15E, GLU15J, GLU90E, GLU90J, GLY122E, GLY122J, GLY129E, GLY129J, GLY155E, GLY155J, GLY16E, GLY16J, GLY20E, GLY20J, GLY98E, GLY98J, GLY99E, GLY99J, HIS21E, HIS21J, ILE100E, ILE100J, LEU153E, LYS154E, LYS154J, LYS174E, LYS174J, LYS178E, LYS178J, LYS180E, LYS180J, MET132E, MET132J, MET93E, MET93J, PHE120E, PHE120J, PHE125E, PHE125J, PHE173E, PHE173J, PRO126E, PRO126J, PRO22E, PRO22J, SER88E, SER88J, SER92E, SER92J, THR104E, THR104J, THR175E, THR175J, THR176E, THR176J, TRP89E, TRP89J, TYR177E, TYR177J, VAL123E, VAL123J, VAL152E, VAL152J, VAL18E, VAL18J
• E-K: ARG135K, GLY165K, GLY166K, GLY167K, LEU163K, LYS202E
• E-L: ASN206E, ASP150L, ASP201E, ASP41E, GLY151L, LYS202E, LYS43E, SER205E, VAL152L, VAL182L, VAL28E
• F-G: ALA160F, ALA160G, ALA213G, ALA215G, ALA69G, ARG149F, ARG149G, ARG170F, ARG170G, ARG221F, ASN158F, ASN158G, ASN94F, ASP150F, ASP150G, ASP156F, ASP156G, ASP172F, ASP172G, ASP187F, ASP187G, ASP192F, ASP192G, ASP97G, CYS171F, CYS171G, GLU140F, GLU140G, GLU144F, GLU144G, GLU196F, GLU211G, GLU214G, GLU218F, GLU218G, GLU35G, GLU96F, GLU96G, GLY151F, GLY155F, GLY155G, GLY166F, GLY166G, GLY167F, GLY167G, GLY36G, HIS168F, HIS168G, HIS189F, HIS189G, HIS193F, HIS193G, HIS194F, HIS194G, HIS212G, HIS216F, HIS216G, ILE195G, LEU146F, LEU146G, LEU161F, LEU161G, LEU163F, LEU163G, LEU219F, LYS138F, LYS145F, LYS145G, LYS154F, LYS154G, LYS174F, LYS174G, LYS37G, LYS70G, MET159F, MET159G, PHE173F, PHE173G, PHE190F, PHE190G, PRO130F, PRO130G, PRO141F, PRO141G, PRO186F, PRO186G, PRO220F, SER142F, SER142G, SER162F, SER162G, SER217F, SER217G, THR143F, THR143G, TRP139F, TRP139G, TYR147F, TYR147G, TYR169F, TYR169G, TYR188F, TYR188G, VAL148F, VAL148G, VAL157F, VAL157G, VAL191F, VAL191G
• F-L: ASN206F, ASP110L, GLU26F, GLU85L, GLY111L, LEU109L, LYS202F, LYS43F, LYS45F, PRO84L
• H-J: ASP187H, GLN184H, GLU85J, VAL182J
• H-K: ALA103H, ALA103K, ALA127H, ALA127K, ALA17H, ALA17K, ARG119H, ARG119K, ARG149H, ARG149K, ARG91H, ARG91K, ASN102H, ASN102K, ASN105H, ASN105K, ASN124H, ASN124K, ASN128H, ASN128K, ASN19H, ASN19K, ASN94H, ASN94K, ASP106H, ASP106K, ASP121H, ASP121K, ASP150H, ASP150K, ASP156H, ASP156K, ASP97H, ASP97K, CYS101H, CYS101K, GLU117H, GLU117K, GLU15H, GLU15K, GLU90H, GLU90K, GLY122H, GLY122K, GLY129H, GLY129K, GLY155H, GLY155K, GLY16H, GLY16K, GLY20H, GLY20K, GLY98H, GLY98K, GLY99H, GLY99K, HIS21H, HIS21K, ILE100H, ILE100K, LEU153H, LYS154H, LYS154K, LYS174H, LYS174K, LYS178H, LYS178K, LYS180H, LYS180K, MET132H, MET132K, MET93H, MET93K, PHE120H, PHE120K, PHE125H, PHE125K, PHE173H, PHE173K, PRO126H, PRO126K, PRO22H, PRO22K, SER88H, SER88K, SER92H, SER92K, THR104H, THR104K, THR175H, THR175K, THR176H, THR176K, TRP89H, TRP89K, TYR177H, TYR177K, VAL123H, VAL123K, VAL152H, VAL152K, VAL18H, VAL18K
• H-L: ASP97H, ASP97L, GLU96H, GLU96L, GLY98H, GLY98L
• I-J: ALA160I, ALA160J, ALA213I, ALA213J, ALA215I, ALA215J, ALA69I, ALA69J, ARG149I, ARG149J, ARG170I, ARG170J, ARG221I, ASN158I, ASN158J, ASN94I, ASN94J, ASP150I, ASP150J, ASP156I, ASP156J, ASP172I, ASP172J, ASP187I, ASP187J, ASP192I, ASP192J, CYS171I, CYS171J, GLU140I, GLU140J, GLU144I, GLU144J, GLU211I, GLU211J, GLU214I, GLU214J, GLU218I, GLU218J, GLU35I, GLU35J, GLU96I, GLU96J, GLY155I, GLY155J, GLY166I, GLY166J, GLY167I, GLY167J, GLY36I, GLY36J, HIS168I, HIS168J, HIS189I, HIS189J, HIS193I, HIS193J, HIS194I, HIS194J, HIS212I, HIS212J, HIS216I, HIS216J, ILE195I, ILE195J, LEU146I, LEU146J, LEU161I, LEU161J, LEU163I, LEU163J, LEU219I, LEU219J, LYS138I, LYS138J, LYS145I, LYS145J, LYS154I, LYS154J, LYS174I, LYS174J, LYS37I, LYS37J, LYS70I, LYS70J, MET159I, MET159J, PHE173I, PHE173J, PHE190I, PHE190J, PRO130J, PRO141I, PRO141J, PRO186I, PRO186J, PRO220I, PRO220J, SER142I, SER142J, SER162I, SER162J, SER217I, SER217J, THR143I, THR143J, TRP139I, TRP139J, TYR147I, TYR147J, TYR169I, TYR169J, TYR188I, TYR188J, VAL148I, VAL148J, VAL157I, VAL157J, VAL191I, VAL191J
• K-L: ALA160K, ALA160L, ARG149K, ARG149L, ARG170K, ARG170L, ASN158K, ASN158L, ASN94K, ASN94L, ASP150K, ASP150L, ASP156K, ASP156L, ASP172K, ASP172L, ASP187K, ASP187L, ASP192K, ASP192L, CYS171K, CYS171L, GLU140K, GLU140L, GLU144K, GLU144L, GLU218L, GLU96K, GLU96L, GLY155K, GLY155L, GLY166K, GLY166L, GLY167K, GLY167L, HIS168K, HIS168L, HIS189K, HIS189L, HIS193K, HIS193L, HIS194K, HIS194L, HIS216K, HIS216L, LEU146K, LEU146L, LEU161K, LEU161L, LEU163K, LEU163L, LYS138K, LYS138L, LYS145K, LYS145L, LYS154K, LYS154L, LYS174K, LYS174L, MET159K, MET159L, PHE173K, PHE173L, PHE190K, PHE190L, PRO130K, PRO130L, PRO141K, PRO141L, PRO186K, PRO186L, SER142K, SER142L, SER162K, SER162L, SER217K, SER217L, THR143K, THR143L, TRP139K, TRP139L, TYR147K, TYR147L, TYR169K, TYR169L, TYR188K, TYR188L, TYR95L, VAL148K, VAL148L, VAL157K, VAL157L, VAL191K, VAL191L