2FRV

crystal structure of the oxidized form of ni-fe hydrogenase

• Total interactions analyzed: 66
• Total true interactions: 21
• Strongest Interaction:
  • Chains: A-B
  • Int. Res.: 307
  • Norm. En. per Res.: -4.5452
• Hub Node: E(4)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
S-L	-215.88	-118.563	-1060.18	-1394.623	307	48	24	41760	10	86	68
S-G	-51.5018	-54.6165	-73.0029	-179.1212	54	5	0	2868	5	26	27
L-C	0.0	0.799	-19.3883	-18.5893	24	0	0	1099	0	4	4
L-D	0.0	-0.5306	-11.5708	-12.1014	33	3	0	907	0	17	16
L-G	0.0	21.0551	-18.9182	2.1368	25	1	1	965	0	17	14
L-J	-2.7963	4.2354	-7.4431	-6.004	32	0	0	567	0	9	10
A-B	-216.1765	-118.5771	-1060.63	-1395.3836	307	48	24	41757	10	86	68
B-E	-24.0591	-32.4474	-48.2546	-104.761	60	8	0	2908	2	32	31
B-H	0.0	4.1584	-6.6514	-2.493	31	0	0	535	0	9	10
B-J	0.0	0.0	-0.0012	-0.0012	2	0	0	3	0	1	1
C-D	-215.9604	-118.5462	-1060.23	-1394.7365	307	48	24	41752	10	86	68
C-I	-50.3576	-54.5514	-67.3144	-172.2234	54	6	0	2842	5	25	27
D-I	-2.3061	22.0326	-18.8956	0.8308	25	1	1	964	0	17	14
E-F	-216.0134	-118.5443	-1060.81	-1395.3677	307	48	24	41760	10	86	68
E-G	-47.2057	-52.6021	-75.5095	-175.3172	55	5	0	2905	5	25	27
E-H	0.0	21.722	-19.7648	1.9572	25	0	1	987	0	17	14
E-J	0.0	0.8002	-19.9241	-19.124	24	0	0	1107	0	4	4
F-J	0.0	-0.5509	-11.403	-11.9539	34	3	0	924	0	16	16
G-H	-216.1864	-118.5651	-1060.62	-1395.3715	307	47	24	41756	10	86	68
G-J	-22.1228	-31.6326	-34.6003	-88.3557	60	6	0	2786	2	31	29
I-J	-215.9492	-118.5496	-1060.88	-1395.3788	307	48	24	41776	10	86	68

Interface residues

• S-L: ALA120S, ALA121S, ALA15S, ALA160L, ALA173L, ALA200S, ALA206L, ALA207L, ALA208S, ALA209L, ALA211L, ALA215L, ALA242S, ALA248S, ALA50S, ALA52S, ALA532L, ALA55S, ALA57L, ALA59S, ALA63S, ALA64L, ARG16L, ARG190S, ARG201L, ARG208L, ARG22L, ARG235L, ARG238L, ARG26S, ARG43L, ARG463L, ARG516L, ARG52L, ARG55L, ARG63L, ARG6S, ARG90S, ASN122L, ASN124S, ASN14S, ASN151S, ASN172L, ASN217L, ASN226S, ASN230L, ASN234S, ASN237S, ASN91L, ASN91S, ASP107L, ASP119L, ASP12L, ASP232L, ASP256S, ASP29S, ASP33S, ASP38S, ASP43S, ASP441L, ASP442L, ASP446L, ASP53L, ASP56L, ASP67S, ASP80S, CYS112S, CYS148S, CYS17S, CYS20S, CYS219S, CYS228L, CYS228S, CYS246S, CYS249S, CYS530L, CYS533L, CYS65L, CYS68L, GLN103L, GLN119S, GLN166L, GLN203L, GLN221L, GLN235S, GLN241S, GLN58L, GLN62L, GLU163L, GLU16S, GLU18L, GLU198L, GLU207S, GLU22S, GLU241L, GLU347L, GLU34S, GLU46L, GLU46S, GLU57S, GLU58S, GLU62S, GLU73S, GLY117S, GLY127S, GLY147S, GLY165L, GLY168L, GLY19L, GLY19S, GLY204S, GLY211S, GLY214L, GLY226L, GLY227L, GLY243S, GLY439L, GLY44L, GLY51S, GLY53S, GLY66L, GLY74S, GLY75S, GLY85S, GLY88S, GLY89S, HIS106L, HIS114L, HIS13S, HIS20L, HIS219L, HIS244S, HIS45S, HIS525L, HIS536L, HIS54S, HIS59L, HIS61S, HIS72L, ILE14L, ILE169L, ILE17L, ILE212L, ILE23L, ILE247S, ILE36S, ILE40S, ILE48L, ILE520L, ILE531L, ILE76S, ILE95S, ILE9L, LEU115L, LEU118L, LEU12S, LEU161L, LEU167L, LEU176L, LEU197L, LEU200L, LEU214S, LEU217S, LEU21L, LEU232S, LEU234L, LEU245L, LEU257S, LEU25S, LEU35S, LEU37S, LEU41L, LEU443L, LEU45L, LEU48S, LEU49L, LEU521L, LEU60S, LYS122S, LYS157L, LYS205L, LYS209S, LYS210S, LYS216L, LYS220S, LYS342L, LYS344L, LYS440L, LYS476L, LYS4S, LYS50L, LYS515L, LYS5S, LYS86S, MET210L, MET38L, MET42S, MET47L, MET49S, MET92S, MET95L, PHE170L, PHE175L, PHE203S, PHE213L, PHE222L, PHE233S, PHE242L, PHE254S, PHE261S, PHE348L, PHE42L, PHE60L, PRO123S, PRO125S, PRO13L, PRO149S, PRO150S, PRO152S, PRO206S, PRO218L, PRO239S, PRO245S, PRO260S, PRO30S, PRO343L, PRO514L, PRO517L, PRO54L, PRO77S, PRO7S, PRO89L, SER164L, SER202S, SER205S, SER21S, SER233L, SER23S, SER259S, SER263S, SER39L, SER41S, SER8S, THR111S, THR126S, THR128S, THR15L, THR171L, THR18S, THR220L, THR223L, THR229L, THR27S, THR341L, THR346L, THR40L, THR445L, THR47S, THR61L, THR69L, TRP238S, TRP255S, TRP327L, TRP345L, TRP356L, TRP447L, TRP84S, TYR113L, TYR11S, TYR174L, TYR182L, TYR196L, TYR212S, TYR231L, TYR258S, TYR262S, TYR31S, TYR328L, TYR444L, TYR44S, TYR83S, VAL10L, VAL110L, VAL118S, VAL11L, VAL121L, VAL123L, VAL154L, VAL158L, VAL162L, VAL184S, VAL202L, VAL204L, VAL224L, VAL225L, VAL236S, VAL240S, VAL24S, VAL28S, VAL32S, VAL518L, VAL535L, VAL56S, VAL67L, VAL77L, VAL87S, VAL9S
• S-G: ALA196G, ALA200G, ALA264G, ALA97S, ARG90S, ASN91S, ASP186G, ASP223G, ASP94S, CYS213G, CYS219G, GLU195G, GLU198G, GLU207G, GLU216G, GLU57S, GLU58S, GLU62S, GLU98S, GLY197G, GLY218G, GLY221G, GLY81S, GLY88S, GLY89S, HIS185G, HIS193G, HIS54S, HIS61S, ILE64S, ILE95S, LEU138S, LEU191G, LEU217G, LEU257G, LYS102S, LYS220G, LYS65S, LYS86S, PHE194G, PHE199G, PRO101S, PRO222G, PRO260G, SER263G, THR183G, THR201G, THR224G, TYR215G, TYR225G, TYR258G, TYR93S, VAL87S, VAL99S
• L-C: ALA121C, ALA272L, ALA403L, ASN124C, ASN270L, ASP256C, ASP391L, ASP80C, GLU402L, GLY127C, GLY398L, GLY400L, LEU394L, LEU397L, LYS122C, LYS269L, LYS395L, PRO123C, PRO125C, PRO401L, THR396L, TRP84C, VAL393L, VAL399L
• L-D: ALA272L, ARG353D, ARG473D, ARG52D, ASN306L, ASP307L, GLU329D, GLU351D, GLU402L, GLU452D, GLU478D, GLY273L, GLY29L, GLY330D, GLY398L, GLY400L, GLY474D, GLY508L, GLY51D, LEU308L, LYS276L, LYS310L, LYS31L, LYS342D, LYS344D, LYS50D, MET47D, PHE405L, PRO401L, PRO510L, SER309L, THR346D, THR451D
• L-G: ALA196G, ARG22L, ASN7L, ASP12L, GLU195G, GLU198G, GLU207G, GLU24L, GLU347L, GLY197G, HIS193G, HIS349L, ILE9L, LYS192G, LYS210G, LYS8L, MET38L, PHE194G, PHE199G, PHE348L, PRO206G, THR40L, TRP345L, TYR212G, VAL10L
• L-J: ALA129L, ALA132L, ALA186L, ARG136L, ARG473J, ARG516L, ASN125L, ASN128L, ASP130L, ASP442J, ASP446J, GLN448J, GLU187L, GLU452J, GLY177L, GLY178L, GLY85J, LEU127L, LYS133L, LYS440J, LYS515L, LYS87J, PRO131L, PRO180L, PRO185L, PRO450J, THR445J, THR451J, TRP447J, TYR449J, VAL84J, VAL86J
• A-B: ALA120A, ALA121A, ALA15A, ALA160B, ALA173B, ALA200A, ALA206B, ALA207B, ALA208A, ALA209B, ALA211B, ALA215B, ALA242A, ALA248A, ALA50A, ALA52A, ALA532B, ALA55A, ALA57B, ALA59A, ALA63A, ALA64B, ARG16B, ARG190A, ARG201B, ARG208B, ARG22B, ARG235B, ARG238B, ARG26A, ARG43B, ARG463B, ARG516B, ARG52B, ARG55B, ARG63B, ARG6A, ARG90A, ASN122B, ASN124A, ASN14A, ASN151A, ASN172B, ASN217B, ASN226A, ASN230B, ASN234A, ASN237A, ASN91A, ASN91B, ASP107B, ASP119B, ASP12B, ASP232B, ASP256A, ASP29A, ASP33A, ASP38A, ASP43A, ASP441B, ASP442B, ASP446B, ASP53B, ASP56B, ASP67A, ASP80A, CYS112A, CYS148A, CYS17A, CYS20A, CYS219A, CYS228A, CYS228B, CYS246A, CYS249A, CYS530B, CYS533B, CYS65B, CYS68B, GLN103B, GLN119A, GLN166B, GLN203B, GLN221B, GLN235A, GLN241A, GLN58B, GLN62B, GLU163B, GLU16A, GLU18B, GLU198B, GLU207A, GLU22A, GLU241B, GLU347B, GLU34A, GLU46A, GLU46B, GLU57A, GLU58A, GLU62A, GLU73A, GLY117A, GLY127A, GLY147A, GLY165B, GLY168B, GLY19A, GLY19B, GLY204A, GLY211A, GLY214B, GLY226B, GLY227B, GLY243A, GLY439B, GLY44B, GLY51A, GLY53A, GLY66B, GLY74A, GLY75A, GLY85A, GLY88A, GLY89A, HIS106B, HIS114B, HIS13A, HIS20B, HIS219B, HIS244A, HIS45A, HIS525B, HIS536B, HIS54A, HIS59B, HIS61A, HIS72B, ILE14B, ILE169B, ILE17B, ILE212B, ILE23B, ILE247A, ILE36A, ILE40A, ILE48B, ILE520B, ILE531B, ILE76A, ILE95A, ILE9B, LEU115B, LEU118B, LEU12A, LEU161B, LEU167B, LEU176B, LEU197B, LEU200B, LEU214A, LEU217A, LEU21B, LEU232A, LEU234B, LEU245B, LEU257A, LEU25A, LEU35A, LEU37A, LEU41B, LEU443B, LEU45B, LEU48A, LEU49B, LEU521B, LEU60A, LYS122A, LYS157B, LYS205B, LYS209A, LYS210A, LYS216B, LYS220A, LYS342B, LYS344B, LYS440B, LYS476B, LYS4A, LYS50B, LYS515B, LYS5A, LYS86A, MET210B, MET38B, MET42A, MET47B, MET49A, MET92A, MET95B, PHE170B, PHE175B, PHE203A, PHE213B, PHE222B, PHE233A, PHE242B, PHE254A, PHE261A, PHE348B, PHE42B, PHE60B, PRO123A, PRO125A, PRO13B, PRO149A, PRO150A, PRO152A, PRO206A, PRO218B, PRO239A, PRO245A, PRO260A, PRO30A, PRO343B, PRO514B, PRO517B, PRO54B, PRO77A, PRO7A, PRO89B, SER164B, SER202A, SER205A, SER21A, SER233B, SER23A, SER259A, SER263A, SER39B, SER41A, SER8A, THR111A, THR126A, THR128A, THR15B, THR171B, THR18A, THR220B, THR223B, THR229B, THR27A, THR341B, THR346B, THR40B, THR445B, THR47A, THR61B, THR69B, TRP238A, TRP255A, TRP327B, TRP345B, TRP356B, TRP447B, TRP84A, TYR113B, TYR11A, TYR174B, TYR182B, TYR196B, TYR212A, TYR231B, TYR258A, TYR262A, TYR31A, TYR328B, TYR444B, TYR44A, TYR83A, VAL10B, VAL110B, VAL118A, VAL11B, VAL121B, VAL123B, VAL154B, VAL158B, VAL162B, VAL184A, VAL202B, VAL204B, VAL224B, VAL225B, VAL236A, VAL240A, VAL24A, VAL28A, VAL32A, VAL518B, VAL535B, VAL56A, VAL67B, VAL77B, VAL87A, VAL9A
• B-E: ALA103E, ALA105E, ALA142E, ALA422B, ALA434B, ALA438B, ARG243B, ARG250B, ASP171E, ASP391B, ASP430B, ASP67E, GLN423B, GLU169E, GLU182E, GLU251B, GLU254B, GLU384B, GLU424B, GLU426B, GLY136E, GLY139E, GLY166E, GLY181E, HIS161E, HIS383B, ILE143E, LEU162E, LEU170E, LEU392B, LEU429B, LEU94B, LYS104E, LYS137E, LYS141E, LYS145B, LYS165E, LYS172E, LYS247B, LYS381B, LYS382B, LYS388B, LYS395B, LYS431B, LYS440B, LYS5E, MET167E, MET178E, PRO168E, PRO385B, THR164E, THR288B, TRP428B, TYR246B, TYR297B, VAL106E, VAL140E, VAL177E, VAL387B, VAL427B
• B-H: ALA129H, ALA132H, ALA186H, ARG136H, ARG473B, ARG516H, ASN125H, ASN128H, ASP130H, ASP442B, ASP446B, GLN448B, GLU187H, GLU452B, GLY177H, GLY178H, GLY85B, LEU127H, LYS133H, LYS440B, LYS515H, LYS87B, PRO131H, PRO180H, PRO185H, PRO450B, THR445B, THR451B, TRP447B, TYR449B, VAL84B
• B-J: ARG136J, LYS310B
• C-D: ALA120C, ALA121C, ALA15C, ALA160D, ALA173D, ALA200C, ALA206D, ALA207D, ALA208C, ALA209D, ALA211D, ALA215D, ALA242C, ALA248C, ALA50C, ALA52C, ALA532D, ALA55C, ALA57D, ALA59C, ALA63C, ALA64D, ARG16D, ARG190C, ARG201D, ARG208D, ARG22D, ARG235D, ARG238D, ARG26C, ARG43D, ARG463D, ARG516D, ARG52D, ARG55D, ARG63D, ARG6C, ARG90C, ASN122D, ASN124C, ASN14C, ASN151C, ASN172D, ASN217D, ASN226C, ASN230D, ASN234C, ASN237C, ASN91C, ASN91D, ASP107D, ASP119D, ASP12D, ASP232D, ASP256C, ASP29C, ASP33C, ASP38C, ASP43C, ASP441D, ASP442D, ASP446D, ASP53D, ASP56D, ASP67C, ASP80C, CYS112C, CYS148C, CYS17C, CYS20C, CYS219C, CYS228C, CYS228D, CYS246C, CYS249C, CYS530D, CYS533D, CYS65D, CYS68D, GLN103D, GLN119C, GLN166D, GLN203D, GLN221D, GLN235C, GLN241C, GLN58D, GLN62D, GLU163D, GLU16C, GLU18D, GLU198D, GLU207C, GLU22C, GLU241D, GLU347D, GLU34C, GLU46C, GLU46D, GLU57C, GLU58C, GLU62C, GLU73C, GLY117C, GLY127C, GLY147C, GLY165D, GLY168D, GLY19C, GLY19D, GLY204C, GLY211C, GLY214D, GLY226D, GLY227D, GLY243C, GLY439D, GLY44D, GLY51C, GLY53C, GLY66D, GLY74C, GLY75C, GLY85C, GLY88C, GLY89C, HIS106D, HIS114D, HIS13C, HIS20D, HIS219D, HIS244C, HIS45C, HIS525D, HIS536D, HIS54C, HIS59D, HIS61C, HIS72D, ILE14D, ILE169D, ILE17D, ILE212D, ILE23D, ILE247C, ILE36C, ILE40C, ILE48D, ILE520D, ILE531D, ILE76C, ILE95C, ILE9D, LEU115D, LEU118D, LEU12C, LEU161D, LEU167D, LEU176D, LEU197D, LEU200D, LEU214C, LEU217C, LEU21D, LEU232C, LEU234D, LEU245D, LEU257C, LEU25C, LEU35C, LEU37C, LEU41D, LEU443D, LEU45D, LEU48C, LEU49D, LEU521D, LEU60C, LYS122C, LYS157D, LYS205D, LYS209C, LYS210C, LYS216D, LYS220C, LYS342D, LYS344D, LYS440D, LYS476D, LYS4C, LYS50D, LYS515D, LYS5C, LYS86C, MET210D, MET38D, MET42C, MET47D, MET49C, MET92C, MET95D, PHE170D, PHE175D, PHE203C, PHE213D, PHE222D, PHE233C, PHE242D, PHE254C, PHE261C, PHE348D, PHE42D, PHE60D, PRO123C, PRO125C, PRO13D, PRO149C, PRO150C, PRO152C, PRO206C, PRO218D, PRO239C, PRO245C, PRO260C, PRO30C, PRO343D, PRO514D, PRO517D, PRO54D, PRO77C, PRO7C, PRO89D, SER164D, SER202C, SER205C, SER21C, SER233D, SER23C, SER259C, SER263C, SER39D, SER41C, SER8C, THR111C, THR126C, THR128C, THR15D, THR171D, THR18C, THR220D, THR223D, THR229D, THR27C, THR341D, THR346D, THR40D, THR445D, THR47C, THR61D, THR69D, TRP238C, TRP255C, TRP327D, TRP345D, TRP356D, TRP447D, TRP84C, TYR113D, TYR11C, TYR174D, TYR182D, TYR196D, TYR212C, TYR231D, TYR258C, TYR262C, TYR31C, TYR328D, TYR444D, TYR44C, TYR83C, VAL10D, VAL110D, VAL118C, VAL11D, VAL121D, VAL123D, VAL154D, VAL158D, VAL162D, VAL184C, VAL202D, VAL204D, VAL224D, VAL225D, VAL236C, VAL240C, VAL24C, VAL28C, VAL32C, VAL518D, VAL535D, VAL56C, VAL67D, VAL77D, VAL87C, VAL9C
• C-I: ALA196I, ALA200I, ALA264I, ALA97C, ARG90C, ASN91C, ASP186I, ASP223I, ASP94C, CYS219I, GLU195I, GLU198I, GLU207I, GLU216I, GLU57C, GLU58C, GLU62C, GLU98C, GLY197I, GLY218I, GLY221I, GLY81C, GLY88C, GLY89C, HIS185I, HIS193I, HIS54C, HIS61C, ILE64C, ILE95C, LEU138C, LEU191I, LEU217I, LEU257I, LYS102C, LYS220I, LYS65C, LYS86C, PHE194I, PHE199I, PRO101C, PRO222I, PRO260I, SER263I, THR183I, THR201I, THR224I, TYR215I, TYR225I, TYR258I, TYR83C, TYR93C, VAL87C, VAL99C
• D-I: ALA196I, ARG22D, ASN7D, ASP12D, GLU195I, GLU198I, GLU207I, GLU24D, GLU347D, GLY197I, HIS193I, HIS349D, ILE9D, LYS192I, LYS210I, LYS8D, MET38D, PHE194I, PHE199I, PHE348D, PRO206I, THR40D, TRP345D, TYR212I, VAL10D
• E-F: ALA120E, ALA121E, ALA15E, ALA160F, ALA173F, ALA200E, ALA206F, ALA207F, ALA208E, ALA209F, ALA211F, ALA215F, ALA242E, ALA248E, ALA50E, ALA52E, ALA532F, ALA55E, ALA57F, ALA59E, ALA63E, ALA64F, ARG16F, ARG190E, ARG201F, ARG208F, ARG22F, ARG235F, ARG238F, ARG26E, ARG43F, ARG463F, ARG516F, ARG52F, ARG55F, ARG63F, ARG6E, ARG90E, ASN122F, ASN124E, ASN14E, ASN151E, ASN172F, ASN217F, ASN226E, ASN230F, ASN234E, ASN237E, ASN91E, ASN91F, ASP107F, ASP119F, ASP12F, ASP232F, ASP256E, ASP29E, ASP33E, ASP38E, ASP43E, ASP441F, ASP442F, ASP446F, ASP53F, ASP56F, ASP67E, ASP80E, CYS112E, CYS148E, CYS17E, CYS20E, CYS219E, CYS228E, CYS228F, CYS246E, CYS249E, CYS530F, CYS533F, CYS65F, CYS68F, GLN103F, GLN119E, GLN166F, GLN203F, GLN221F, GLN235E, GLN241E, GLN58F, GLN62F, GLU163F, GLU16E, GLU18F, GLU198F, GLU207E, GLU22E, GLU241F, GLU347F, GLU34E, GLU46E, GLU46F, GLU57E, GLU58E, GLU62E, GLU73E, GLY117E, GLY127E, GLY147E, GLY165F, GLY168F, GLY19E, GLY19F, GLY204E, GLY211E, GLY214F, GLY226F, GLY227F, GLY243E, GLY439F, GLY44F, GLY51E, GLY53E, GLY66F, GLY74E, GLY75E, GLY85E, GLY88E, GLY89E, HIS106F, HIS114F, HIS13E, HIS20F, HIS219F, HIS244E, HIS45E, HIS525F, HIS536F, HIS54E, HIS59F, HIS61E, HIS72F, ILE14F, ILE169F, ILE17F, ILE212F, ILE23F, ILE247E, ILE36E, ILE40E, ILE48F, ILE520F, ILE531F, ILE76E, ILE95E, ILE9F, LEU115F, LEU118F, LEU12E, LEU161F, LEU167F, LEU176F, LEU197F, LEU200F, LEU214E, LEU217E, LEU21F, LEU232E, LEU234F, LEU245F, LEU257E, LEU25E, LEU35E, LEU37E, LEU41F, LEU443F, LEU45F, LEU48E, LEU49F, LEU521F, LEU60E, LYS122E, LYS157F, LYS205F, LYS209E, LYS210E, LYS216F, LYS220E, LYS342F, LYS344F, LYS440F, LYS476F, LYS4E, LYS50F, LYS515F, LYS5E, LYS86E, MET210F, MET38F, MET42E, MET47F, MET49E, MET92E, MET95F, PHE170F, PHE175F, PHE203E, PHE213F, PHE222F, PHE233E, PHE242F, PHE254E, PHE261E, PHE348F, PHE42F, PHE60F, PRO123E, PRO125E, PRO13F, PRO149E, PRO150E, PRO152E, PRO206E, PRO218F, PRO239E, PRO245E, PRO260E, PRO30E, PRO343F, PRO514F, PRO517F, PRO54F, PRO77E, PRO7E, PRO89F, SER164F, SER202E, SER205E, SER21E, SER233F, SER23E, SER259E, SER263E, SER39F, SER41E, SER8E, THR111E, THR126E, THR128E, THR15F, THR171F, THR18E, THR220F, THR223F, THR229F, THR27E, THR341F, THR346F, THR40F, THR445F, THR47E, THR61F, THR69F, TRP238E, TRP255E, TRP327F, TRP345F, TRP356F, TRP447F, TRP84E, TYR113F, TYR11E, TYR174F, TYR182F, TYR196F, TYR212E, TYR231F, TYR258E, TYR262E, TYR31E, TYR328F, TYR444F, TYR44E, TYR83E, VAL10F, VAL110F, VAL118E, VAL11F, VAL121F, VAL123F, VAL154F, VAL158F, VAL162F, VAL184E, VAL202F, VAL204F, VAL224F, VAL225F, VAL236E, VAL240E, VAL24E, VAL28E, VAL32E, VAL518F, VAL535F, VAL56E, VAL67F, VAL77F, VAL87E, VAL9E
• E-G: ALA196E, ALA200E, ALA264E, ALA97G, ARG90G, ASN91G, ASP186E, ASP223E, ASP94G, CYS213E, CYS219E, GLU195E, GLU198E, GLU207E, GLU216E, GLU57G, GLU58G, GLU62G, GLU98G, GLY197E, GLY218E, GLY221E, GLY81G, GLY88G, GLY89G, HIS185E, HIS193E, HIS54G, HIS61G, ILE64G, ILE95G, LEU138G, LEU191E, LEU217E, LEU257E, LYS102G, LYS220E, LYS65G, LYS86G, PHE194E, PHE199E, PRO101G, PRO222E, PRO260E, SER263E, THR183E, THR201E, THR224E, TYR215E, TYR225E, TYR258E, TYR83G, TYR93G, VAL87G, VAL99G
• E-H: ALA196E, ARG22H, ASN7H, ASP12H, GLU195E, GLU198E, GLU207E, GLU24H, GLU347H, GLY197E, HIS193E, HIS349H, ILE9H, LYS192E, LYS210E, LYS8H, MET38H, PHE194E, PHE199E, PHE348H, PRO206E, THR40H, TRP345H, TYR212E, VAL10H
• E-J: ALA121E, ALA272J, ALA403J, ASN124E, ASN270J, ASP256E, ASP391J, ASP80E, GLU402J, GLY127E, GLY398J, GLY400J, LEU394J, LEU397J, LYS122E, LYS269J, LYS395J, PRO123E, PRO125E, PRO401J, THR396J, TRP84E, VAL393J, VAL399J
• F-J: ALA272J, ARG353F, ARG473F, ARG52F, ASN306J, ASP307J, GLU329F, GLU351F, GLU402J, GLU452F, GLU478F, GLY273J, GLY29J, GLY330F, GLY398J, GLY400J, GLY474F, GLY508J, GLY51F, LEU308J, LYS276J, LYS310J, LYS31J, LYS342F, LYS344F, LYS476F, LYS50F, MET47F, PHE405J, PRO401J, PRO510J, SER309J, THR346F, THR451F
• G-H: ALA120G, ALA121G, ALA15G, ALA160H, ALA173H, ALA200G, ALA206H, ALA207H, ALA208G, ALA209H, ALA211H, ALA215H, ALA242G, ALA248G, ALA50G, ALA52G, ALA532H, ALA55G, ALA57H, ALA59G, ALA63G, ALA64H, ARG16H, ARG190G, ARG201H, ARG208H, ARG22H, ARG235H, ARG238H, ARG26G, ARG43H, ARG463H, ARG516H, ARG52H, ARG55H, ARG63H, ARG6G, ARG90G, ASN122H, ASN124G, ASN14G, ASN151G, ASN172H, ASN217H, ASN226G, ASN230H, ASN234G, ASN237G, ASN91G, ASN91H, ASP107H, ASP119H, ASP12H, ASP232H, ASP256G, ASP29G, ASP33G, ASP38G, ASP43G, ASP441H, ASP442H, ASP446H, ASP53H, ASP56H, ASP67G, ASP80G, CYS112G, CYS148G, CYS17G, CYS20G, CYS219G, CYS228G, CYS228H, CYS246G, CYS249G, CYS530H, CYS533H, CYS65H, CYS68H, GLN103H, GLN119G, GLN166H, GLN203H, GLN221H, GLN235G, GLN241G, GLN58H, GLN62H, GLU163H, GLU16G, GLU18H, GLU198H, GLU207G, GLU22G, GLU241H, GLU347H, GLU34G, GLU46G, GLU46H, GLU57G, GLU58G, GLU62G, GLU73G, GLY117G, GLY127G, GLY147G, GLY165H, GLY168H, GLY19G, GLY19H, GLY204G, GLY211G, GLY214H, GLY226H, GLY227H, GLY243G, GLY439H, GLY44H, GLY51G, GLY53G, GLY66H, GLY74G, GLY75G, GLY85G, GLY88G, GLY89G, HIS106H, HIS114H, HIS13G, HIS20H, HIS219H, HIS244G, HIS45G, HIS525H, HIS536H, HIS54G, HIS59H, HIS61G, HIS72H, ILE14H, ILE169H, ILE17H, ILE212H, ILE23H, ILE247G, ILE36G, ILE40G, ILE48H, ILE520H, ILE531H, ILE76G, ILE95G, ILE9H, LEU115H, LEU118H, LEU12G, LEU161H, LEU167H, LEU176H, LEU197H, LEU200H, LEU214G, LEU217G, LEU21H, LEU232G, LEU234H, LEU245H, LEU257G, LEU25G, LEU35G, LEU37G, LEU41H, LEU443H, LEU45H, LEU48G, LEU49H, LEU521H, LEU60G, LYS122G, LYS157H, LYS205H, LYS209G, LYS210G, LYS216H, LYS220G, LYS342H, LYS344H, LYS440H, LYS476H, LYS4G, LYS50H, LYS515H, LYS5G, LYS86G, MET210H, MET38H, MET42G, MET47H, MET49G, MET92G, MET95H, PHE170H, PHE175H, PHE203G, PHE213H, PHE222H, PHE233G, PHE242H, PHE254G, PHE261G, PHE348H, PHE42H, PHE60H, PRO123G, PRO125G, PRO13H, PRO149G, PRO150G, PRO152G, PRO206G, PRO218H, PRO239G, PRO245G, PRO260G, PRO30G, PRO343H, PRO514H, PRO517H, PRO54H, PRO77G, PRO7G, PRO89H, SER164H, SER202G, SER205G, SER21G, SER233H, SER23G, SER259G, SER263G, SER39H, SER41G, SER8G, THR111G, THR126G, THR128G, THR15H, THR171H, THR18G, THR220H, THR223H, THR229H, THR27G, THR341H, THR346H, THR40H, THR445H, THR47G, THR61H, THR69H, TRP238G, TRP255G, TRP327H, TRP345H, TRP356H, TRP447H, TRP84G, TYR113H, TYR11G, TYR174H, TYR182H, TYR196H, TYR212G, TYR231H, TYR258G, TYR262G, TYR31G, TYR328H, TYR444H, TYR44G, TYR83G, VAL10H, VAL110H, VAL118G, VAL11H, VAL121H, VAL123H, VAL154H, VAL158H, VAL162H, VAL184G, VAL202H, VAL204H, VAL224H, VAL225H, VAL236G, VAL240G, VAL24G, VAL28G, VAL32G, VAL518H, VAL535H, VAL56G, VAL67H, VAL77H, VAL87G, VAL9G
• G-J: ALA103G, ALA105G, ALA142G, ALA422J, ALA434J, ALA438J, ARG243J, ARG250J, ASP171G, ASP391J, ASP430J, ASP67G, GLN423J, GLU169G, GLU182G, GLU251J, GLU254J, GLU384J, GLU424J, GLU426J, GLY136G, GLY139G, GLY166G, GLY181G, HIS161G, HIS383J, ILE143G, LEU162G, LEU170G, LEU392J, LEU429J, LEU94J, LYS104G, LYS137G, LYS141G, LYS145J, LYS165G, LYS172G, LYS247J, LYS381J, LYS382J, LYS388J, LYS395J, LYS431J, LYS440J, LYS5G, MET167G, MET178G, PRO168G, PRO385J, THR164G, THR288J, TRP428J, TYR246J, TYR297J, VAL106G, VAL140G, VAL177G, VAL387J, VAL427J
• I-J: ALA120I, ALA121I, ALA15I, ALA160J, ALA173J, ALA200I, ALA206J, ALA207J, ALA208I, ALA209J, ALA211J, ALA215J, ALA242I, ALA248I, ALA50I, ALA52I, ALA532J, ALA55I, ALA57J, ALA59I, ALA63I, ALA64J, ARG16J, ARG190I, ARG201J, ARG208J, ARG22J, ARG235J, ARG238J, ARG26I, ARG43J, ARG463J, ARG516J, ARG52J, ARG55J, ARG63J, ARG6I, ARG90I, ASN122J, ASN124I, ASN14I, ASN151I, ASN172J, ASN217J, ASN226I, ASN230J, ASN234I, ASN237I, ASN91I, ASN91J, ASP107J, ASP119J, ASP12J, ASP232J, ASP256I, ASP29I, ASP33I, ASP38I, ASP43I, ASP441J, ASP442J, ASP446J, ASP53J, ASP56J, ASP67I, ASP80I, CYS112I, CYS148I, CYS17I, CYS20I, CYS219I, CYS228I, CYS228J, CYS246I, CYS249I, CYS530J, CYS533J, CYS65J, CYS68J, GLN103J, GLN119I, GLN166J, GLN203J, GLN221J, GLN235I, GLN241I, GLN58J, GLN62J, GLU163J, GLU16I, GLU18J, GLU198J, GLU207I, GLU22I, GLU241J, GLU347J, GLU34I, GLU46I, GLU46J, GLU57I, GLU58I, GLU62I, GLU73I, GLY117I, GLY127I, GLY147I, GLY165J, GLY168J, GLY19I, GLY19J, GLY204I, GLY211I, GLY214J, GLY226J, GLY227J, GLY243I, GLY439J, GLY44J, GLY51I, GLY53I, GLY66J, GLY74I, GLY75I, GLY85I, GLY88I, GLY89I, HIS106J, HIS114J, HIS13I, HIS20J, HIS219J, HIS244I, HIS45I, HIS525J, HIS536J, HIS54I, HIS59J, HIS61I, HIS72J, ILE14J, ILE169J, ILE17J, ILE212J, ILE23J, ILE247I, ILE36I, ILE40I, ILE48J, ILE520J, ILE531J, ILE76I, ILE95I, ILE9J, LEU115J, LEU118J, LEU12I, LEU161J, LEU167J, LEU176J, LEU197J, LEU200J, LEU214I, LEU217I, LEU21J, LEU232I, LEU234J, LEU245J, LEU257I, LEU25I, LEU35I, LEU37I, LEU41J, LEU443J, LEU45J, LEU48I, LEU49J, LEU521J, LEU60I, LYS122I, LYS157J, LYS205J, LYS209I, LYS210I, LYS216J, LYS220I, LYS342J, LYS344J, LYS440J, LYS476J, LYS4I, LYS50J, LYS515J, LYS5I, LYS86I, MET210J, MET38J, MET42I, MET47J, MET49I, MET92I, MET95J, PHE170J, PHE175J, PHE203I, PHE213J, PHE222J, PHE233I, PHE242J, PHE254I, PHE261I, PHE348J, PHE42J, PHE60J, PRO123I, PRO125I, PRO13J, PRO149I, PRO150I, PRO152I, PRO206I, PRO218J, PRO239I, PRO245I, PRO260I, PRO30I, PRO343J, PRO514J, PRO517J, PRO54J, PRO77I, PRO7I, PRO89J, SER164J, SER202I, SER205I, SER21I, SER233J, SER23I, SER259I, SER263I, SER39J, SER41I, SER8I, THR111I, THR126I, THR128I, THR15J, THR171J, THR18I, THR220J, THR223J, THR229J, THR27I, THR341J, THR346J, THR40J, THR445J, THR47I, THR61J, THR69J, TRP238I, TRP255I, TRP327J, TRP345J, TRP356J, TRP447J, TRP84I, TYR113J, TYR11I, TYR174J, TYR182J, TYR196J, TYR212I, TYR231J, TYR258I, TYR262I, TYR31I, TYR328J, TYR444J, TYR44I, TYR83I, VAL10J, VAL110J, VAL118I, VAL11J, VAL121J, VAL123J, VAL154J, VAL158J, VAL162J, VAL184I, VAL202J, VAL204J, VAL224J, VAL225J, VAL236I, VAL240I, VAL24I, VAL28I, VAL32I, VAL518J, VAL535J, VAL56I, VAL67J, VAL77J, VAL87I, VAL9I