2C9I

structure of the fluorescent protein asfp499 from anemonia sulcata

• Total interactions analyzed: 28
• Total true interactions: 18
• Strongest Interaction:
  • Chains: A-B
  • Int. Res.: 156
  • Norm. En. per Res.: -3.4808
• Hub Node: A(6)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
A-B	-84.0133	2.2102	-461.2	-543.0031	156	25	9	18272	8	55	56
A-C	0.0	2.0623	-2.1532	-0.0909	14	0	0	190	0	0	4
A-D	-21.4953	-2.2164	-268.455	-292.1667	109	7	3	10292	0	14	18
A-F	0.0	0.0	-19.2274	-19.2274	19	5	0	777	0	8	7
A-G	-16.4695	-1.6226	-43.4905	-61.5826	42	1	0	1994	2	19	16
A-H	0.0	0.0	-0.0203	-0.0203	6	0	0	13	0	2	0
B-C	-24.1723	-3.5659	-270.981	-298.7191	108	14	3	10640	0	14	18
B-D	0.0	0.0	-1.4127	-1.4127	13	0	0	140	0	0	4
B-F	0.0	0.0	-0.0012	-0.0012	3	0	0	3	0	2	4
C-D	-78.4513	-4.7526	-445.854	-529.0579	155	21	9	17822	5	58	54
D-E	0.0	0.0	-0.0194	-0.0194	2	0	0	2	0	2	3
D-F	-9.7029	5.8656	-15.924	-19.7613	27	1	0	979	1	6	14
E-F	-65.1455	-11.1653	-455.17	-531.4808	156	29	9	18006	7	56	54
E-G	0.0	2.0444	-2.2656	-0.2213	13	0	0	198	0	0	4
E-H	-22.4467	-3.942	-278.968	-305.3567	105	14	3	10735	0	14	18
F-G	-22.2706	-2.1689	-270.589	-295.0285	110	13	4	10552	0	14	17
F-H	0.0	2.1092	-2.7292	-0.62	11	0	0	215	0	0	4
G-H	-72.7349	-7.7139	-447.575	-528.0238	153	33	9	17695	5	55	54

Interface residues

• A-B: ALA161A, ALA161B, ALA216A, ALA216B, ARG150A, ARG150B, ARG157A, ARG157B, ARG195A, ARG195B, ASN200A, ASN200B, ASN228A, ASN228B, ASP151A, ASP151B, ASP158A, ASP158B, ASP98A, ASP98B, CYS138A, CYS138B, CYS172A, CYS172B, GLN220A, GLN220B, GLN39A, GLN39B, GLU141A, GLU141B, GLU145A, GLU145B, GLU175A, GLU175B, GLU188A, GLU188B, GLU197A, GLU197B, GLU209A, GLU209B, GLU212A, GLU212B, GLU97A, GLU97B, GLY139A, GLY139B, GLY167A, GLY167B, GLY168A, GLY168B, GLY226A, GLY226B, GLY37A, GLY37B, HIS169A, HIS169B, HIS190A, HIS190B, HIS192A, HIS192B, HIS193A, HIS193B, HIS211A, HIS211B, HIS227A, HIS227B, ILE148A, ILE148B, ILE201A, LEU155A, LEU155B, LEU156A, LEU156B, LEU162A, LEU162B, LEU164A, LEU164B, LEU170A, LEU170B, LEU189A, LEU189B, LEU194A, LEU194B, LEU199A, LEU199B, LEU225A, LEU225B, LYS198A, LYS198B, LYS224A, LYS224B, LYS71A, LYS71B, MET163A, MET163B, MET174A, MET174B, MET196A, MET196B, PHE173A, PHE173B, PHE191A, PHE191B, PRO142A, PRO142B, PRO149A, PRO149B, PRO160A, PRO160B, PRO187A, PRO187B, PRO222A, PRO222B, SER143A, SER143B, SER171A, SER171B, SER213A, SER213B, SER217A, SER217B, SER219A, SER219B, SER223A, SER223B, SER40A, SER40B, SER94A, THR144A, THR144B, THR146A, THR146B, THR159A, THR159B, THR176A, THR176B, THR38A, THR38B, THR95A, THR95B, TRP140A, TRP140B, TYR218A, TYR218B, TYR96A, TYR96B, VAL147A, VAL147B, VAL214A, VAL214B, VAL215A, VAL215B, VAL221A, VAL221B, VAL93A, VAL93B
• A-C: ASN125A, ASN125C, ASP98A, ASP98C, GLU97A, GLU97C, GLY99A, GLY99C, PHE173A, PHE173C, PRO127A, PRO127C, TYR96A, TYR96C
• A-D: ALA104A, ALA104D, ALA128A, ALA128D, ALA23A, ALA23D, ARG150A, ARG150D, ARG157A, ARG157D, ARG92A, ARG92D, ASN125A, ASN125D, ASN129A, ASN129D, ASN20A, ASN20D, ASP151A, ASP151D, ASP98A, ASP98D, GLN106A, GLN106D, GLU107A, GLU107D, GLU16A, GLU16D, GLU175A, GLU175D, GLU91A, GLU91D, GLY100A, GLY100D, GLY123A, GLY123D, GLY153A, GLY153D, GLY17A, GLY17D, GLY99A, GLY99D, HIS22A, HIS22D, LEU102A, LEU102D, LEU122A, LEU122D, LEU154A, LEU154D, LEU155A, LEU155D, LEU156A, LEU156D, LYS120A, LYS120D, LYS179A, LYS179D, LYS181A, LYS181D, LYS25A, MET133A, MET174A, MET174D, PHE126A, PHE126D, PHE24A, PHE24D, PHE79A, PHE88A, PHE88D, PRO127A, PRO127D, SER103A, SER103D, SER180A, SER180D, SER18A, SER18D, SER89A, SER89D, SER94A, SER94D, THR105A, THR105D, THR124A, THR124D, THR176A, THR176D, THR177A, THR177D, THR95A, THR95D, TRP90A, TRP90D, TYR178A, TYR178D, TYR21A, TYR21D, VAL101A, VAL101D, VAL119A, VAL119D, VAL121A, VAL121D, VAL19A, VAL19D, VAL93A, VAL93D
• A-F: ALA128F, ASN129F, ASN20F, ASP151A, GLN134F, GLU183A, GLY130F, GLY152A, LYS135F, LYS136F, LYS179A, LYS181A, LYS182A, MET133F, PRO50F, PRO52F, SER180A, TYR21F, VAL184A
• A-G: ARG150G, ASN228A, ASP151G, ASP78A, GLN82A, GLU183A, GLU183G, GLU188A, GLU75A, GLY152G, GLY153G, GLY87G, ILE76A, LEU154G, LEU186A, LEU186G, LYS179G, LYS181G, LYS182A, LYS182G, LYS185A, LYS185G, LYS74A, LYS81A, MET1A, PHE79A, PHE79G, PHE88G, PRO149G, PRO187A, PRO3A, PRO73A, PRO77A, SER180G, SER83G, SER89G, TYR178G, TYR218A, TYR2A, TYR72A, VAL184A, VAL184G
• A-H: ASP166H, GLY167H, GLY168H, LYS182A, PRO85A, SER4A
• B-C: ALA104B, ALA104C, ALA128B, ALA128C, ALA23B, ALA23C, ARG150B, ARG150C, ARG157B, ARG157C, ARG92B, ARG92C, ASN125B, ASN125C, ASN129B, ASN129C, ASN20B, ASN20C, ASP151B, ASP151C, ASP98B, ASP98C, GLN106B, GLN106C, GLU107B, GLU107C, GLU16B, GLU16C, GLU175B, GLU175C, GLU91B, GLU91C, GLY100B, GLY100C, GLY123B, GLY123C, GLY153B, GLY153C, GLY17B, GLY17C, GLY99B, GLY99C, HIS22B, HIS22C, LEU102B, LEU102C, LEU122B, LEU122C, LEU154B, LEU154C, LEU155B, LEU155C, LEU156B, LEU156C, LYS120B, LYS120C, LYS179B, LYS179C, LYS181C, LYS25B, MET133B, MET133C, MET174B, MET174C, PHE126B, PHE126C, PHE24B, PHE79C, PHE88B, PHE88C, PRO127B, PRO127C, SER103B, SER103C, SER180B, SER180C, SER18B, SER18C, SER89B, SER89C, SER94B, SER94C, THR105B, THR105C, THR124B, THR124C, THR176B, THR176C, THR177B, THR177C, THR95B, THR95C, TRP90B, TRP90C, TYR178B, TYR178C, TYR21B, TYR21C, VAL101B, VAL101C, VAL119B, VAL119C, VAL121B, VAL121C, VAL19B, VAL19C, VAL93B, VAL93C
• B-D: ASN125B, ASN125D, ASP98B, ASP98D, GLU97B, GLU97D, GLY99B, GLY99D, PHE173B, PHE173D, PRO127D, TYR96B, TYR96D
• B-F: ASP166B, GLY49F, PRO50F
• C-D: ALA161C, ALA161D, ALA216C, ALA216D, ARG150C, ARG150D, ARG157C, ARG157D, ARG195C, ARG195D, ASN200C, ASN200D, ASN228C, ASN228D, ASP151C, ASP151D, ASP158C, ASP158D, ASP98C, ASP98D, CYS138C, CYS138D, CYS172C, CYS172D, GLN210C, GLN220C, GLN220D, GLN39C, GLN39D, GLU141C, GLU141D, GLU145C, GLU145D, GLU175C, GLU175D, GLU188C, GLU188D, GLU197C, GLU197D, GLU209C, GLU209D, GLU212C, GLU212D, GLU97C, GLU97D, GLY139C, GLY139D, GLY167C, GLY167D, GLY168C, GLY168D, GLY226C, GLY226D, GLY37C, GLY37D, HIS169C, HIS169D, HIS190C, HIS190D, HIS192C, HIS192D, HIS193C, HIS193D, HIS211C, HIS211D, HIS227C, HIS227D, ILE148C, ILE148D, ILE201C, ILE201D, LEU155C, LEU155D, LEU156C, LEU156D, LEU162C, LEU162D, LEU164D, LEU170C, LEU170D, LEU189C, LEU189D, LEU194C, LEU194D, LEU199C, LEU199D, LEU225C, LEU225D, LYS198C, LYS198D, LYS224C, LYS224D, LYS71C, LYS71D, MET163C, MET163D, MET174C, MET174D, MET196C, MET196D, PHE173C, PHE173D, PHE191C, PHE191D, PRO142C, PRO142D, PRO149C, PRO149D, PRO160C, PRO160D, PRO187C, PRO187D, PRO222C, PRO222D, SER143C, SER143D, SER171C, SER171D, SER213C, SER213D, SER217D, SER219C, SER219D, SER223C, SER223D, SER40C, SER40D, THR144C, THR144D, THR146C, THR146D, THR159C, THR159D, THR176C, THR176D, THR38C, THR38D, THR95C, THR95D, TRP140C, TRP140D, TYR218C, TYR218D, TYR96C, TYR96D, VAL147C, VAL147D, VAL214C, VAL214D, VAL215C, VAL215D, VAL221C, VAL221D, VAL93C, VAL93D
• D-E: ASP203D, GLY226E
• D-F: ALA128D, ALA165D, ALA165F, ASN129D, ASN200F, ASP166D, ASP166F, ASP203F, ASP204F, CYS138F, GLN134D, GLY130D, GLY167F, HIS22D, ILE201F, LEU164D, LYS135D, LYS135F, LYS136D, LYS136F, PRO131D, PRO50D, PRO50F, PRO52D, SER202F, THR207F, TRP205F
• E-F: ALA161E, ALA161F, ALA216E, ARG150E, ARG150F, ARG157E, ARG157F, ARG195E, ARG195F, ASN200E, ASN200F, ASN228E, ASN228F, ASP151E, ASP151F, ASP158E, ASP158F, ASP98E, ASP98F, CYS138E, CYS138F, CYS172E, CYS172F, GLN210F, GLN220E, GLN220F, GLN39E, GLN39F, GLU141E, GLU141F, GLU145E, GLU145F, GLU175E, GLU175F, GLU188E, GLU188F, GLU197E, GLU197F, GLU209E, GLU209F, GLU212E, GLU212F, GLU36F, GLU97E, GLU97F, GLY139E, GLY139F, GLY167E, GLY167F, GLY168E, GLY168F, GLY226E, GLY226F, GLY37E, GLY37F, HIS169E, HIS169F, HIS190E, HIS190F, HIS192E, HIS192F, HIS193E, HIS193F, HIS211E, HIS211F, HIS227E, HIS227F, ILE148E, ILE148F, ILE201E, ILE201F, LEU155E, LEU155F, LEU156E, LEU156F, LEU162E, LEU162F, LEU164E, LEU164F, LEU170E, LEU170F, LEU189E, LEU189F, LEU194E, LEU194F, LEU199E, LEU199F, LEU225E, LEU225F, LYS198E, LYS198F, LYS224E, LYS224F, LYS71E, LYS71F, MET163E, MET163F, MET174E, MET174F, MET196E, MET196F, PHE173E, PHE173F, PHE191E, PHE191F, PRO142E, PRO142F, PRO149E, PRO149F, PRO160E, PRO160F, PRO187E, PRO187F, PRO222E, PRO222F, SER143E, SER143F, SER171E, SER171F, SER213E, SER213F, SER217E, SER219E, SER219F, SER223E, SER223F, SER40E, SER40F, THR144E, THR144F, THR146E, THR146F, THR159E, THR159F, THR176E, THR176F, THR38E, THR38F, THR95E, THR95F, TRP140E, TRP140F, TYR218E, TYR218F, TYR96E, TYR96F, VAL147E, VAL147F, VAL214E, VAL214F, VAL215E, VAL215F, VAL221E, VAL221F, VAL93E, VAL93F
• E-G: ASN125E, ASN125G, ASP98E, ASP98G, GLU97E, GLU97G, GLY99E, GLY99G, PHE173E, PHE173G, PRO127E, TYR96E, TYR96G
• E-H: ALA104E, ALA104H, ALA128E, ALA128H, ALA23E, ALA23H, ARG150E, ARG150H, ARG157E, ARG157H, ARG92E, ARG92H, ASN125E, ASN125H, ASN129E, ASN129H, ASN20E, ASN20H, ASP151E, ASP151H, ASP98H, GLN106E, GLN106H, GLU107E, GLU107H, GLU16E, GLU16H, GLU175E, GLU175H, GLU91E, GLU91H, GLY100E, GLY100H, GLY123E, GLY123H, GLY153E, GLY153H, GLY17E, GLY17H, GLY99E, GLY99H, HIS22E, HIS22H, LEU102E, LEU102H, LEU122E, LEU122H, LEU154E, LEU154H, LEU155E, LEU155H, LEU156E, LEU156H, LYS118E, LYS120E, LYS120H, LYS179E, LYS179H, MET133E, MET174E, MET174H, PHE126E, PHE126H, PHE24E, PHE79H, PHE88E, PHE88H, PRO127E, PRO127H, SER103E, SER103H, SER180E, SER180H, SER18E, SER18H, SER89E, SER89H, SER94E, SER94H, THR105E, THR105H, THR124E, THR124H, THR176E, THR176H, THR177E, THR177H, THR95E, THR95H, TRP90E, TRP90H, TYR178E, TYR178H, TYR21E, TYR21H, VAL101E, VAL101H, VAL119E, VAL119H, VAL121E, VAL121H, VAL19E, VAL19H, VAL93E, VAL93H
• F-G: ALA104F, ALA104G, ALA128F, ALA128G, ALA23F, ALA23G, ARG150F, ARG150G, ARG157F, ARG157G, ARG92F, ARG92G, ASN125F, ASN125G, ASN129F, ASN129G, ASN20F, ASN20G, ASP151F, ASP151G, ASP98F, ASP98G, GLN106F, GLN106G, GLU107F, GLU107G, GLU16F, GLU16G, GLU175F, GLU175G, GLU91F, GLU91G, GLY100F, GLY100G, GLY123F, GLY123G, GLY153F, GLY153G, GLY17F, GLY17G, GLY99F, GLY99G, HIS22F, HIS22G, LEU102F, LEU102G, LEU122F, LEU122G, LEU154F, LEU154G, LEU155F, LEU155G, LEU156F, LEU156G, LYS120F, LYS120G, LYS179F, LYS179G, LYS181F, LYS181G, LYS25F, LYS25G, MET133F, MET174F, MET174G, PHE126F, PHE126G, PHE24F, PHE24G, PHE79F, PHE88F, PHE88G, PRO127F, PRO127G, SER103F, SER103G, SER180F, SER180G, SER18F, SER18G, SER89F, SER89G, SER94F, SER94G, THR105F, THR105G, THR124F, THR124G, THR176F, THR176G, THR177F, THR177G, THR95F, THR95G, TRP90F, TRP90G, TYR178F, TYR178G, TYR21F, TYR21G, VAL101F, VAL101G, VAL119F, VAL119G, VAL121F, VAL121G, VAL19F, VAL19G, VAL93F, VAL93G
• F-H: ASN125H, ASP98F, ASP98H, GLU97F, GLU97H, GLY99F, GLY99H, PHE173F, PRO127F, TYR96F, TYR96H
• G-H: ALA161G, ALA161H, ALA216H, ARG150G, ARG150H, ARG157G, ARG157H, ARG195G, ARG195H, ASN200G, ASN200H, ASN228G, ASN228H, ASP151G, ASP151H, ASP158G, ASP158H, ASP98G, ASP98H, CYS138G, CYS138H, CYS172G, CYS172H, GLN210H, GLN220G, GLN220H, GLN39G, GLN39H, GLU141G, GLU141H, GLU145G, GLU145H, GLU175G, GLU175H, GLU188G, GLU188H, GLU197G, GLU197H, GLU209G, GLU209H, GLU212G, GLU212H, GLU97G, GLU97H, GLY139G, GLY139H, GLY167G, GLY167H, GLY168G, GLY168H, GLY226G, GLY226H, GLY37G, GLY37H, HIS169G, HIS169H, HIS190G, HIS190H, HIS192G, HIS192H, HIS193G, HIS193H, HIS211G, HIS211H, HIS227G, HIS227H, ILE148G, ILE148H, ILE201G, LEU155G, LEU155H, LEU156G, LEU156H, LEU162G, LEU162H, LEU164H, LEU170G, LEU170H, LEU189G, LEU189H, LEU194G, LEU194H, LEU199G, LEU199H, LEU225G, LEU225H, LYS198G, LYS198H, LYS224G, LYS224H, LYS71G, LYS71H, MET163G, MET163H, MET174G, MET174H, MET196G, MET196H, PHE173G, PHE173H, PHE191G, PHE191H, PRO142G, PRO142H, PRO149G, PRO149H, PRO160G, PRO160H, PRO187G, PRO187H, PRO222G, PRO222H, SER143G, SER143H, SER171G, SER171H, SER213G, SER213H, SER217H, SER219G, SER219H, SER223G, SER223H, SER40G, SER40H, THR144G, THR144H, THR146G, THR146H, THR159G, THR159H, THR176G, THR176H, THR38G, THR38H, THR95G, THR95H, TRP140G, TRP140H, TYR218G, TYR218H, TYR96G, TYR96H, VAL147G, VAL147H, VAL214G, VAL214H, VAL215G, VAL215H, VAL221G, VAL221H, VAL93G, VAL93H