1TG6

crystallography and mutagenesis point to an essential role for the n- terminus of human mitochondrial clpp

• Total interactions analyzed: 21
• Total true interactions: 12
• Strongest Interaction:
  • Chains: C-D
  • Int. Res.: 131
  • Norm. En. per Res.: -3.0862
• Hub Node: A(4)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
A-B	-10.6625	-41.517	-327.33	-379.5095	135	16	11	13668	3	24	16
A-C	0.0	0.0	-0.0069	-0.0069	3	0	0	6	0	0	0
A-F	0.0	0.0	-0.0132	-0.0132	4	0	0	11	0	0	0
A-G	-18.9098	-36.8325	-321.799	-377.5414	136	16	14	14376	3	24	18
B-C	-20.3465	-41.437	-328.534	-390.3174	129	21	12	13916	3	25	17
B-G	0.0	0.0	-0.0004	-0.0004	2	0	0	1	0	0	0
C-D	-27.2117	-56.188	-320.894	-404.2937	131	32	11	13788	4	25	17
D-E	-12.0016	-43.5439	-350.358	-405.9035	138	15	16	14987	3	26	17
D-F	0.0	0.0	-0.0068	-0.0068	2	0	0	9	0	0	0
E-F	-12.5159	-51.5934	-370.894	-435.0033	146	19	12	15393	4	30	19
E-G	0.0	0.0	-0.12	-0.12	4	0	0	48	0	0	0
F-G	-18.9625	-41.2614	-16.951	-77.175	142	59	13	15787	3	27	17

Interface residues

• A-B: ALA132A, ALA139A, ALA167B, ALA40A, ALA45A, ALA72A, ALA75A, ALA95B, ALA96B, ARG118B, ARG170B, ARG172B, ARG22A, ARG22B, ARG25A, ARG25B, ASN116B, ASN151A, ASN55A, ASN64B, ASN85A, ASP134A, ASP171B, ASP18A, ASP18B, ASP36A, ASP37A, ASP78A, CYS30A, CYS30B, CYS91B, GLN131A, GLN138A, GLN148A, GLN46A, GLN51A, GLN81A, GLN94B, GLU140A, GLU141A, GLU169B, GLU178B, GLU26B, GLU53A, GLY130A, GLY33B, GLY67B, GLY73A, GLY93B, HIS112B, HIS122B, HIS156A, HIS191B, ILE119B, ILE135A, ILE137A, ILE142A, ILE152A, ILE19A, ILE19B, ILE28A, ILE28B, ILE3A, ILE3B, ILE44A, ILE5A, ILE5B, ILE63B, ILE76A, ILE83A, LEU101A, LEU114B, LEU145A, LEU149A, LEU189B, LEU23A, LEU23B, LEU24A, LEU2A, LEU2B, LEU42A, LEU47A, LEU48A, LEU50A, LEU74A, LEU84A, LYS144A, LYS155A, MET120B, MET174B, MET32A, MET32B, MET80A, MET98A, PHE49A, PRO115B, PRO176B, PRO192B, PRO1A, PRO1B, PRO34B, PRO4B, PRO66B, SER113B, SER117B, SER175B, SER21A, SER38A, SER41A, SER52A, SER65B, THR133A, THR71A, THR79A, TRP90B, TYR173B, TYR17A, TYR17B, TYR20A, TYR20B, TYR62B, TYR77A, TYR82A, VAL188B, VAL190B, VAL29A, VAL31B, VAL39A, VAL43A, VAL69A, VAL6B, VAL70A, VAL7A, VAL7B, VAL92B
• A-C: LEU42A, PRO1C, SER38A
• A-F: LEU2A, LEU42F, PRO1A, SER38F
• A-G: ALA132G, ALA139G, ALA40G, ALA45G, ALA72G, ALA75G, ALA95A, ALA96A, ARG118A, ARG170A, ARG172A, ARG22A, ARG22G, ARG25A, ARG25G, ASN116A, ASN151G, ASN55G, ASN64A, ASP134G, ASP171A, ASP18A, ASP18G, ASP36G, ASP37G, ASP78G, CYS30A, CYS30G, CYS91A, GLN131G, GLN138G, GLN148G, GLN46G, GLN51G, GLN81G, GLN94A, GLU140G, GLU141G, GLU169A, GLU178A, GLU26A, GLU53G, GLY130G, GLY33A, GLY33G, GLY67A, GLY73G, GLY93A, HIS112A, HIS122A, HIS156G, HIS191A, HIS60A, ILE119A, ILE135G, ILE137G, ILE142G, ILE152G, ILE19A, ILE19G, ILE28A, ILE28G, ILE3A, ILE3G, ILE44G, ILE5A, ILE5G, ILE63A, ILE76G, ILE83G, LEU101G, LEU114A, LEU145G, LEU149G, LEU189A, LEU23A, LEU23G, LEU24G, LEU2A, LEU2G, LEU42G, LEU47G, LEU48G, LEU50G, LEU74G, LEU84G, LYS144G, LYS155G, MET120A, MET174A, MET32A, MET32G, MET80G, MET98G, PHE49G, PRO115A, PRO176A, PRO192A, PRO193A, PRO1A, PRO1G, PRO34A, PRO34G, PRO4A, PRO66A, SER113A, SER117A, SER175A, SER21G, SER38G, SER41G, SER52G, SER65A, THR133G, THR71G, THR79G, TRP90A, TYR173A, TYR17A, TYR17G, TYR20A, TYR20G, TYR62A, TYR77G, TYR82G, VAL188A, VAL190A, VAL29G, VAL31A, VAL39G, VAL43G, VAL69G, VAL6A, VAL70G, VAL7A, VAL92A
• B-C: ALA132B, ALA40B, ALA45B, ALA72B, ALA75B, ALA95C, ALA96C, ARG118C, ARG170C, ARG172C, ARG22B, ARG22C, ARG25B, ARG25C, ASN116C, ASN151B, ASN55B, ASN64C, ASN85B, ASP134B, ASP171C, ASP18B, ASP18C, ASP36B, ASP37B, ASP78B, CYS30B, CYS30C, CYS91C, GLN131B, GLN138B, GLN148B, GLN46B, GLN51B, GLN81B, GLN94C, GLU140B, GLU141B, GLU169C, GLU178C, GLU26C, GLU53B, GLY130B, GLY33C, GLY67C, GLY73B, GLY93C, HIS112C, HIS122C, HIS156B, HIS191C, ILE119C, ILE135B, ILE137B, ILE142B, ILE152B, ILE19C, ILE28C, ILE3B, ILE3C, ILE44B, ILE5B, ILE5C, ILE63C, ILE76B, ILE83B, LEU101B, LEU114C, LEU145B, LEU149B, LEU189C, LEU23C, LEU24B, LEU2B, LEU2C, LEU42B, LEU47B, LEU48B, LEU50B, LEU74B, LEU84B, LYS144B, LYS155B, MET120C, MET174C, MET32B, MET32C, MET80B, MET98B, PHE49B, PRO115C, PRO176C, PRO192C, PRO1B, PRO1C, PRO34C, PRO4C, PRO66C, SER113C, SER117C, SER175C, SER21B, SER38B, SER41B, SER52B, SER65C, THR133B, THR71B, THR79B, TRP90C, TYR173C, TYR17B, TYR17C, TYR20B, TYR20C, TYR62C, TYR77B, TYR82B, VAL188C, VAL190C, VAL29B, VAL31C, VAL39B, VAL43B, VAL69B, VAL6C, VAL70B, VAL7C, VAL92C
• B-G: LEU42G, PRO1B
• C-D: ALA132C, ALA139C, ALA40C, ALA45C, ALA72C, ALA75C, ALA95D, ALA96D, ARG118D, ARG170D, ARG172D, ARG22C, ARG22D, ARG25C, ARG25D, ASN116D, ASN151C, ASN64D, ASN85C, ASP134C, ASP171D, ASP18C, ASP18D, ASP36C, ASP37C, ASP78C, CYS30C, CYS30D, CYS91D, GLN131C, GLN138C, GLN148C, GLN46C, GLN51C, GLN81C, GLN94D, GLU140C, GLU141C, GLU169D, GLU178D, GLU26D, GLU53C, GLY130C, GLY33D, GLY67D, GLY73C, GLY93D, HIS112D, HIS122D, HIS156C, HIS191D, ILE119D, ILE135C, ILE137C, ILE142C, ILE152C, ILE19C, ILE19D, ILE28D, ILE3C, ILE3D, ILE44C, ILE5C, ILE5D, ILE76C, ILE83C, LEU101C, LEU114D, LEU145C, LEU149C, LEU189D, LEU23D, LEU24C, LEU2C, LEU2D, LEU42C, LEU47C, LEU48C, LEU50C, LEU74C, LEU84C, LYS144C, LYS155C, MET120D, MET174D, MET32D, MET80C, MET98C, PHE49C, PRO108C, PRO115D, PRO176D, PRO192D, PRO193D, PRO1D, PRO34D, PRO4D, PRO66D, SER113D, SER117D, SER175D, SER21C, SER38C, SER41C, SER52C, SER65D, THR107C, THR133C, THR71C, THR79C, TRP90D, TYR173D, TYR17C, TYR17D, TYR20C, TYR20D, TYR62D, TYR77C, TYR82C, VAL188D, VAL190D, VAL29C, VAL31D, VAL39C, VAL43C, VAL69C, VAL6C, VAL6D, VAL70C, VAL7D, VAL92D
• D-E: ALA132D, ALA40D, ALA45D, ALA72D, ALA75D, ALA95E, ALA96E, ARG118E, ARG170E, ARG172E, ARG22D, ARG22E, ARG25D, ARG25E, ASN116E, ASN151D, ASN55D, ASN64E, ASN85D, ASP134D, ASP171E, ASP18D, ASP18E, ASP195E, ASP36D, ASP37D, ASP78D, CYS30D, CYS30E, CYS91E, GLN131D, GLN138D, GLN148D, GLN194E, GLN46D, GLN51D, GLN81D, GLN94E, GLU140D, GLU141D, GLU169E, GLU178E, GLU197E, GLU26E, GLU53D, GLY130D, GLY196E, GLY33E, GLY73D, GLY93E, HIS112E, HIS122E, HIS156D, HIS191E, HIS60E, ILE119E, ILE135D, ILE137D, ILE142D, ILE152D, ILE19E, ILE28D, ILE28E, ILE3D, ILE3E, ILE44D, ILE5E, ILE63E, ILE76D, ILE83D, LEU101D, LEU114E, LEU145D, LEU149D, LEU189E, LEU202E, LEU23D, LEU23E, LEU24D, LEU2D, LEU2E, LEU42D, LEU47D, LEU48D, LEU50D, LEU74D, LEU84D, LYS144D, LYS155D, LYS56D, MET120E, MET174E, MET32D, MET32E, MET80D, PHE49D, PRO115E, PRO176E, PRO192E, PRO193E, PRO1D, PRO1E, PRO200E, PRO34E, PRO4E, PRO66E, SER113E, SER117E, SER175E, SER21D, SER38D, SER41D, SER52D, SER65E, THR133D, THR201E, THR71D, THR79D, TRP90E, TYR173E, TYR17D, TYR17E, TYR20D, TYR20E, TYR62E, TYR77D, TYR82D, VAL188E, VAL190E, VAL29D, VAL31E, VAL39D, VAL43D, VAL69D, VAL6E, VAL70D, VAL7E, VAL92E
• D-F: PRO1F, SER38D
• E-F: ALA104E, ALA105E, ALA132E, ALA139E, ALA40E, ALA45E, ALA72E, ALA75E, ALA95F, ALA96F, ARG111E, ARG118F, ARG170F, ARG172F, ARG22E, ARG22F, ARG25E, ASN116F, ASN151E, ASN55E, ASN64F, ASN85E, ASP134E, ASP171F, ASP18E, ASP18F, ASP195F, ASP36E, ASP37E, ASP78E, CYS30E, CYS30F, CYS91F, GLN131E, GLN138E, GLN148E, GLN194F, GLN46E, GLN51E, GLN81E, GLN94F, GLU140E, GLU141E, GLU169F, GLU178F, GLU26F, GLU53E, GLY106E, GLY109E, GLY130E, GLY196F, GLY33E, GLY33F, GLY73E, GLY93F, HIS112F, HIS122F, HIS156E, HIS191F, ILE119F, ILE135E, ILE137E, ILE142E, ILE152E, ILE19F, ILE28E, ILE28F, ILE35E, ILE3E, ILE3F, ILE44E, ILE5F, ILE63F, ILE76E, ILE83E, ILE87E, LEU101E, LEU114F, LEU145E, LEU149E, LEU189F, LEU23F, LEU24E, LEU2E, LEU2F, LEU42E, LEU47E, LEU48E, LEU50E, LEU74E, LEU84E, LYS144E, LYS155E, LYS158E, LYS56E, MET110E, MET120F, MET174F, MET32E, MET32F, MET80E, PHE49E, PRO108E, PRO115F, PRO176F, PRO192F, PRO193F, PRO1F, PRO34E, PRO34F, PRO4F, PRO66F, PRO86E, SER113F, SER117F, SER175F, SER21E, SER38E, SER41E, SER52E, SER65F, THR107E, THR133E, THR71E, THR79E, TRP90F, TYR173F, TYR17E, TYR17F, TYR20E, TYR20F, TYR62F, TYR77E, TYR82E, VAL188F, VAL190F, VAL29E, VAL31E, VAL31F, VAL39E, VAL43E, VAL69E, VAL6F, VAL70E, VAL7F, VAL92F
• E-G: LEU42E, PRO1G, SER38E, SER41E
• F-G: ALA132F, ALA40F, ALA45F, ALA72F, ALA75F, ALA95G, ALA96G, ARG118G, ARG170G, ARG172G, ARG22F, ARG22G, ARG25F, ARG25G, ASN116G, ASN151F, ASN55F, ASN64G, ASN85F, ASP134F, ASP171G, ASP18F, ASP18G, ASP195G, ASP36F, ASP37F, ASP78F, CYS30F, CYS30G, CYS91G, GLN131F, GLN138F, GLN148F, GLN194G, GLN46F, GLN51F, GLN81F, GLN94G, GLU140F, GLU141F, GLU169G, GLU178G, GLU197G, GLU26G, GLU53F, GLY130F, GLY196G, GLY33G, GLY73F, GLY93G, HIS112G, HIS122G, HIS156F, HIS191G, HIS60G, ILE119G, ILE135F, ILE137F, ILE142F, ILE152F, ILE19F, ILE19G, ILE28F, ILE28G, ILE3F, ILE3G, ILE44F, ILE5F, ILE5G, ILE63G, ILE76F, ILE83F, LEU101F, LEU114G, LEU145F, LEU149F, LEU189G, LEU202G, LEU23F, LEU23G, LEU24F, LEU2F, LEU2G, LEU42F, LEU47F, LEU48F, LEU50F, LEU74F, LEU84F, LYS144F, LYS155F, LYS56F, MET120G, MET174G, MET32F, MET32G, MET80F, PHE49F, PRO115G, PRO176G, PRO192G, PRO193G, PRO1F, PRO1G, PRO200G, PRO34G, PRO4F, PRO4G, PRO66G, SER113G, SER117G, SER175G, SER21F, SER38F, SER41F, SER52F, SER65G, THR133F, THR201G, THR71F, THR79F, TRP90G, TYR173G, TYR17F, TYR17G, TYR20F, TYR20G, TYR62G, TYR77F, TYR82F, VAL188G, VAL190G, VAL29F, VAL31G, VAL39F, VAL43F, VAL69F, VAL6F, VAL6G, VAL70F, VAL7G, VAL92G