1SZO

crystal structure analysis of the 6-oxo camphor hydrolase his122ala mutant bound to its natural product (2s,4s)- alpha-campholinic acid

• Total interactions analyzed: 66
• Total true interactions: 33
• Strongest Interaction:
  • Chains: I-L
  • Int. Res.: 122
  • Norm. En. per Res.: -4.1878
• Hub Node: A(5)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
A-B	-49.1742	-50.3614	-353.989	-453.5246	117	12	17	14495	6	34	41
A-C	-60.973	-54.3663	-330.399	-445.7383	115	14	17	14162	6	34	40
A-D	-72.3944	-186.1275	-267.918	-526.4399	135	10	2	10981	9	71	59
A-E	-6.7861	-7.1649	-140.554	-154.5049	57	0	11	5757	2	14	17
A-F	0.0	0.0	-1.2055	-1.2055	4	0	0	97	0	0	4
B-C	-55.8851	-53.0053	-337.545	-446.4353	116	13	16	14233	6	33	42
B-D	0.0	0.0	-1.1254	-1.1254	4	0	0	95	0	0	4
B-E	-83.6284	-182.1367	-254.502	-520.2671	134	9	2	10571	10	66	59
B-F	0.0	-1.5338	-136.026	-137.5598	57	0	11	5712	0	15	17
B-G	-18.154	-4.3488	-52.6638	-75.1666	33	4	0	1654	2	14	22
C-D	-6.6126	-8.8468	-144.262	-159.7214	57	2	11	5821	2	14	17
C-E	0.0	0.0	-1.0398	-1.0398	4	0	0	91	0	0	4
C-F	-78.0896	-188.6359	-269.939	-536.6645	133	10	1	10813	8	66	59
D-E	-51.5491	-57.3508	-336.843	-445.7429	120	9	17	14579	6	33	42
D-F	-60.9252	-51.5487	-334.907	-447.3809	117	12	16	14163	6	34	40
E-F	-63.4598	-48.5754	-335.563	-447.5981	119	13	16	14481	6	34	42
E-G	0.0	0.0	-0.0003	-0.0003	2	0	0	1	0	0	0
E-J	0.0	2.6799	-11.9856	-9.3057	20	0	0	431	0	8	12
G-H	-48.4481	-51.9061	-348.455	-448.8092	120	11	16	14793	6	33	43
G-I	-56.386	-50.0099	-322.35	-428.7459	113	11	16	14329	6	33	42
G-J	-84.9628	-137.5599	-262.548	-485.0707	141	13	2	10589	7	66	59
G-K	0.0	-9.1497	-139.533	-148.6827	59	0	11	5759	1	15	17
G-L	0.0	0.0	-1.0585	-1.0585	4	0	0	84	0	0	4
H-I	-49.909	-51.9218	-340.407	-442.2378	117	9	17	14601	6	33	42
H-J	0.0	0.0	-1.1784	-1.1784	4	0	0	90	0	0	4
H-K	-81.862	-187.2593	-270.764	-539.8853	137	8	2	11139	9	66	57
H-L	0.0	-5.5187	-135.153	-140.6717	56	0	11	5658	2	13	17
I-J	0.0	-7.0148	-141.908	-148.9228	58	0	11	5804	1	15	17
I-K	0.0	0.0	-1.3033	-1.3033	4	0	0	94	0	0	4
I-L	-86.5412	-189.6679	-234.7	-510.9091	122	15	0	9506	9	66	58
J-K	-48.05	-53.21	-334.53	-435.7899	119	10	17	14171	6	33	41
J-L	-62.0185	-50.7881	-358.134	-470.9406	122	13	16	14741	6	34	42
K-L	-64.918	-48.0895	-338.165	-451.1725	117	10	16	14299	6	34	41

Interface residues

• A-B: ALA113B, ALA156A, ALA184B, ALA189B, ALA211B, ALA217B, ALA221B, ALA235A, ALA242A, ALA245A, ALA247A, ALA248A, ARG168A, ARG168B, ARG170A, ARG182B, ARG202B, ARG218B, ARG219B, ARG222B, ARG227B, ARG230B, ARG231A, ARG231B, ASN167A, ASN191B, ASP130B, ASP142A, ASP154A, ASP186A, ASP186B, ASP236A, ASP250A, GLN177A, GLN228A, GLN228B, GLU108B, GLU192B, GLU212B, GLU234A, GLU244A, GLY149A, GLY153A, GLY155A, GLY165A, GLY165B, GLY169A, GLY176A, GLY188B, GLY209B, GLY240A, HIS145A, HIS157A, HIS162A, HIS162B, HIS243A, ILE112B, ILE131B, ILE150A, ILE210B, ILE249A, LEU104B, LEU105B, LEU133B, LEU164A, LEU164B, LEU173A, LEU174A, LEU185B, LEU194B, LEU206B, LEU215B, LEU216B, LEU225B, LEU229B, LEU237A, LEU239A, LEU241A, LEU246A, LEU251A, LYS213B, LYS223B, MET128B, MET233A, PHE146A, PHE172A, PRO110B, PRO126B, PRO147A, PRO152A, PRO161A, PRO214B, SER129B, SER148A, SER166A, SER238A, THR175A, THR183B, THR226B, TRP160A, TRP160B, TYR171A, TYR187A, TYR187B, TYR220B, VAL109B, VAL111B, VAL127B, VAL132B, VAL151A, VAL158A, VAL159B, VAL163B, VAL190B, VAL224B, VAL232A, VAL63B
• A-C: ALA156C, ALA184A, ALA189A, ALA211A, ALA217A, ALA221A, ALA235C, ALA242C, ALA245C, ALA247C, ALA248C, ARG168A, ARG168C, ARG170C, ARG182A, ARG202A, ARG218A, ARG219A, ARG222A, ARG227A, ARG230A, ARG231A, ARG231C, ASN167C, ASN191A, ASP130A, ASP154C, ASP186A, ASP186C, ASP236C, ASP250C, GLN177C, GLN228A, GLN228C, GLU108A, GLU192A, GLU212A, GLU234C, GLU244C, GLY149C, GLY153C, GLY155C, GLY165A, GLY165C, GLY169C, GLY176C, GLY188A, GLY209A, GLY240C, HIS145C, HIS157C, HIS162A, HIS162C, HIS243C, ILE112A, ILE131A, ILE150C, ILE210A, ILE249C, LEU104A, LEU105A, LEU133A, LEU164A, LEU164C, LEU173C, LEU174C, LEU185A, LEU194A, LEU206A, LEU215A, LEU216A, LEU225A, LEU229A, LEU237C, LEU239C, LEU241C, LEU246C, LEU251C, LYS213A, LYS223A, MET128A, MET233C, PHE146C, PHE172C, PRO110A, PRO126A, PRO147C, PRO152C, PRO161C, PRO214A, SER129A, SER148C, SER166C, SER238C, THR175C, THR183A, THR226A, TRP160A, TRP160C, TYR171C, TYR187A, TYR187C, TYR220A, VAL109A, VAL111A, VAL127A, VAL132A, VAL151C, VAL158C, VAL159A, VAL163A, VAL190A, VAL224A, VAL232C, VAL63A
• A-D: ALA235A, ALA235D, ALA242A, ALA242D, ALA245A, ALA245D, ALA44A, ALA49A, ALA49D, ALA56A, ALA56D, ALA5A, ALA5D, ARG219A, ARG219D, ARG227A, ARG227D, ARG230A, ARG230D, ARG231A, ARG231D, ARG59A, ARG59D, ARG99A, ARG99D, ASN102A, ASN102D, ASN103A, ASN103D, ASP236A, ASP236D, ASP46A, ASP46D, ASP54A, ASP54D, ASP58A, ASP58D, ASP89A, ASP89D, ASP91A, ASP91D, CYS51A, CYS51D, CYS57A, CYS57D, GLN228A, GLN228D, GLN3D, GLN98A, GLN98D, GLU108A, GLU108D, GLU16A, GLU234A, GLU234D, GLU47A, GLU47D, GLU92A, GLU92D, GLU96A, GLU96D, GLY240A, GLY240D, HIS45A, HIS53A, HIS53D, HIS88A, HIS88D, ILE107A, ILE107D, ILE249A, ILE249D, ILE55A, ILE55D, ILE93A, ILE93D, ILE94A, ILE94D, LEU105A, LEU105D, LEU215A, LEU215D, LEU216A, LEU216D, LEU229A, LEU229D, LEU237A, LEU237D, LEU239A, LEU239D, LEU241A, LEU241D, LEU246A, LEU246D, LEU48A, LEU4A, LEU4D, LYS14A, LYS14D, LYS223A, LYS223D, LYS2D, MET233A, MET233D, PHE95A, PHE95D, PRO87A, PRO87D, SER106A, SER106D, SER238A, SER238D, SER42A, THR226A, THR226D, THR43A, THR43D, THR6A, THR6D, THR86A, THR86D, TRP90A, TRP90D, TYR15A, TYR15D, TYR220A, TYR220D, TYR50A, TYR50D, VAL109A, VAL109D, VAL224A, VAL224D, VAL232A, VAL232D
• A-E: ALA235A, ALA235E, ALA242A, ALA242E, ALA245A, ALA245E, ALA247A, ALA247E, ALA248A, ALA248E, ASP236A, ASP236E, ASP250A, ASP250E, ASP89A, ASP89E, ASP91A, ASP91E, GLN98A, GLN98E, GLU234A, GLU234E, GLU244A, GLU244E, GLY240A, GLY240E, GLY252A, GLY85A, GLY85E, HIS243A, HIS243E, HIS88A, HIS88E, ILE249A, ILE249E, ILE94A, ILE94E, LEU237A, LEU237E, LEU239A, LEU239E, LEU241A, LEU241E, LEU246A, LEU246E, LEU251A, LEU251E, PHE95A, PHE95E, PRO87A, PRO87E, SER238A, SER238E, THR86A, THR86E, TRP90A, TRP90E
• A-F: ARG227A, ARG227F, ARG231A, ARG231F
• B-C: ALA156B, ALA184C, ALA189C, ALA211C, ALA217C, ALA221C, ALA235B, ALA242B, ALA245B, ALA247B, ALA248B, ARG168B, ARG168C, ARG170B, ARG182C, ARG202C, ARG218C, ARG219C, ARG222C, ARG227C, ARG230C, ARG231B, ARG231C, ASN167B, ASN191C, ASP130C, ASP142B, ASP154B, ASP186B, ASP186C, ASP236B, ASP250B, GLN177B, GLN228B, GLN228C, GLU108C, GLU192C, GLU212C, GLU234B, GLU244B, GLY149B, GLY153B, GLY155B, GLY165B, GLY165C, GLY169B, GLY176B, GLY188C, GLY209C, GLY240B, HIS145B, HIS157B, HIS162B, HIS162C, HIS243B, ILE112C, ILE131C, ILE150B, ILE210C, ILE249B, LEU104C, LEU105C, LEU133C, LEU164B, LEU164C, LEU173B, LEU174B, LEU185C, LEU194C, LEU206C, LEU215C, LEU216C, LEU225C, LEU229C, LEU237B, LEU239B, LEU241B, LEU246B, LEU251B, LYS213C, LYS223C, MET128C, MET233B, PHE146B, PHE172B, PRO110C, PRO126C, PRO147B, PRO152B, PRO161B, PRO214C, SER129C, SER148B, SER166B, SER238B, THR175B, THR183C, THR226C, TRP160B, TRP160C, TYR171B, TYR187B, TYR187C, TYR220C, VAL109C, VAL111C, VAL127C, VAL132C, VAL151B, VAL158B, VAL159C, VAL163C, VAL190C, VAL224C, VAL232B, VAL63C
• B-D: ARG227B, ARG227D, ARG231B, ARG231D
• B-E: ALA235B, ALA235E, ALA242B, ALA242E, ALA245B, ALA245E, ALA44E, ALA49B, ALA49E, ALA56B, ALA56E, ALA5B, ALA5E, ARG219B, ARG219E, ARG227B, ARG227E, ARG230B, ARG230E, ARG231B, ARG231E, ARG59B, ARG59E, ARG99B, ARG99E, ASN102B, ASN102E, ASN103B, ASN103E, ASP236B, ASP236E, ASP46B, ASP46E, ASP54B, ASP54E, ASP58B, ASP58E, ASP89B, ASP89E, ASP91B, ASP91E, CYS51B, CYS51E, CYS57B, CYS57E, GLN228B, GLN228E, GLN98B, GLN98E, GLU108B, GLU108E, GLU16E, GLU234B, GLU234E, GLU47B, GLU47E, GLU92B, GLU92E, GLU96B, GLU96E, GLY240B, GLY240E, HIS45E, HIS53B, HIS53E, HIS88B, HIS88E, ILE107B, ILE107E, ILE18E, ILE249B, ILE249E, ILE55B, ILE55E, ILE93B, ILE93E, ILE94B, ILE94E, LEU105B, LEU105E, LEU215B, LEU215E, LEU216B, LEU216E, LEU229B, LEU229E, LEU237B, LEU237E, LEU239B, LEU239E, LEU241B, LEU241E, LEU246B, LEU246E, LEU48E, LEU4B, LEU4E, LYS14B, LYS14E, LYS223B, LYS223E, MET233B, MET233E, PHE95B, PHE95E, PRO87B, PRO87E, SER106B, SER106E, SER238B, SER238E, SER42E, THR226B, THR226E, THR43B, THR43E, THR6B, THR6E, THR86B, THR86E, TRP90B, TRP90E, TYR15B, TYR15E, TYR220B, TYR220E, TYR50B, TYR50E, VAL109B, VAL109E, VAL224B, VAL224E, VAL232B, VAL232E
• B-F: ALA235B, ALA235F, ALA242B, ALA242F, ALA245B, ALA245F, ALA247B, ALA247F, ALA248B, ALA248F, ASP236B, ASP236F, ASP250B, ASP250F, ASP89B, ASP89F, ASP91B, ASP91F, GLN98B, GLN98F, GLU234B, GLU234F, GLU244B, GLU244F, GLY240B, GLY240F, GLY252F, GLY85B, GLY85F, HIS243B, HIS243F, HIS88B, HIS88F, ILE249B, ILE249F, ILE94B, ILE94F, LEU237B, LEU237F, LEU239B, LEU239F, LEU241B, LEU241F, LEU246B, LEU246F, LEU251B, LEU251F, PHE95B, PHE95F, PRO87B, PRO87F, SER238B, SER238F, THR86B, THR86F, TRP90B, TRP90F
• B-G: ARG19B, ARG208G, ARG22G, ASN17B, ASP23G, GLN13B, GLN13G, GLN9G, GLU10B, GLU10G, GLU16B, GLU21G, GLU34B, GLU47B, GLU60G, GLY24G, GLY25G, GLY35B, HIS32B, ILE18B, LEU20B, LYS14B, LYS36B, PHE8G, PRO7G, SER12B, THR33B, THR43B, TRP204G, TYR11B, TYR15B, VAL26G, VAL39B
• C-D: ALA235C, ALA235D, ALA242C, ALA242D, ALA245C, ALA245D, ALA247C, ALA247D, ALA248C, ALA248D, ASP236C, ASP236D, ASP250C, ASP250D, ASP89C, ASP89D, ASP91C, ASP91D, GLN98C, GLN98D, GLU234C, GLU234D, GLU244C, GLU244D, GLY240C, GLY240D, GLY252D, GLY85C, GLY85D, HIS243C, HIS243D, HIS88C, HIS88D, ILE249C, ILE249D, ILE94C, ILE94D, LEU237C, LEU237D, LEU239C, LEU239D, LEU241C, LEU241D, LEU246C, LEU246D, LEU251C, LEU251D, PHE95C, PHE95D, PRO87C, PRO87D, SER238C, SER238D, THR86C, THR86D, TRP90C, TRP90D
• C-E: ARG227C, ARG227E, ARG231C, ARG231E
• C-F: ALA235C, ALA235F, ALA242C, ALA242F, ALA245C, ALA245F, ALA44F, ALA49C, ALA49F, ALA56C, ALA56F, ALA5C, ALA5F, ARG219C, ARG219F, ARG227C, ARG227F, ARG230C, ARG230F, ARG231C, ARG231F, ARG59C, ARG59F, ARG99C, ARG99F, ASN102C, ASN102F, ASN103C, ASN103F, ASN17F, ASP236C, ASP236F, ASP46C, ASP46F, ASP54C, ASP54F, ASP58C, ASP58F, ASP89C, ASP89F, ASP91C, ASP91F, CYS51F, CYS57C, CYS57F, GLN228C, GLN228F, GLN98C, GLN98F, GLU108C, GLU108F, GLU16F, GLU234C, GLU234F, GLU47F, GLU92C, GLU92F, GLU96C, GLU96F, GLY240C, GLY240F, HIS45F, HIS53C, HIS53F, HIS88C, HIS88F, ILE107C, ILE107F, ILE18F, ILE249C, ILE249F, ILE55C, ILE55F, ILE93C, ILE93F, ILE94C, ILE94F, LEU104C, LEU105C, LEU105F, LEU215C, LEU215F, LEU216C, LEU216F, LEU229C, LEU229F, LEU237C, LEU237F, LEU239C, LEU239F, LEU241C, LEU241F, LEU246C, LEU246F, LEU48F, LEU4C, LYS14C, LYS14F, LYS223C, LYS223F, MET233C, MET233F, PHE52C, PHE95C, PHE95F, PRO87C, PRO87F, SER106C, SER106F, SER238C, SER238F, SER42F, THR226C, THR226F, THR43F, THR6C, THR6F, THR86C, THR86F, TRP90C, TRP90F, TYR15C, TYR15F, TYR220C, TYR220F, TYR50C, TYR50F, VAL109C, VAL109F, VAL224C, VAL224F, VAL232C, VAL232F
• D-E: ALA113D, ALA156E, ALA184D, ALA189D, ALA211D, ALA217D, ALA221D, ALA235E, ALA242E, ALA245E, ALA247E, ALA248E, ARG168D, ARG168E, ARG170E, ARG182D, ARG202D, ARG218D, ARG219D, ARG222D, ARG227D, ARG230D, ARG231D, ARG231E, ASN167E, ASN191D, ASP130D, ASP154E, ASP186D, ASP186E, ASP236E, ASP250E, GLN177E, GLN228D, GLN228E, GLU108D, GLU192D, GLU212D, GLU234E, GLU244E, GLY149E, GLY153E, GLY155E, GLY165D, GLY165E, GLY169E, GLY176E, GLY188D, GLY209D, GLY240E, HIS145E, HIS157E, HIS162D, HIS162E, HIS243E, ILE112D, ILE131D, ILE150E, ILE210D, ILE249E, LEU104D, LEU105D, LEU133D, LEU164D, LEU164E, LEU173E, LEU174E, LEU185D, LEU194D, LEU206D, LEU215D, LEU216D, LEU225D, LEU229D, LEU229E, LEU237E, LEU239E, LEU241E, LEU246E, LEU251E, LYS213D, LYS223D, MET128D, MET233E, PHE146E, PHE172E, PRO110D, PRO126D, PRO147E, PRO152E, PRO161D, PRO161E, PRO214D, SER129D, SER148E, SER166E, SER238E, THR175E, THR183D, THR226D, TRP160D, TRP160E, TYR171E, TYR187D, TYR187E, TYR220D, VAL109D, VAL111D, VAL127D, VAL132D, VAL151E, VAL158E, VAL159D, VAL159E, VAL163D, VAL163E, VAL190D, VAL224D, VAL232E, VAL63D
• D-F: ALA113F, ALA156D, ALA184F, ALA189F, ALA211F, ALA217F, ALA221F, ALA235D, ALA242D, ALA245D, ALA247D, ALA248D, ARG168D, ARG168F, ARG170D, ARG182F, ARG202F, ARG218F, ARG219F, ARG222F, ARG227F, ARG230F, ARG231D, ARG231F, ASN167D, ASN191F, ASP130F, ASP154D, ASP186D, ASP186F, ASP236D, ASP250D, GLN177D, GLN228D, GLN228F, GLU108F, GLU192F, GLU212F, GLU234D, GLU244D, GLY149D, GLY153D, GLY155D, GLY165D, GLY165F, GLY169D, GLY176D, GLY188F, GLY209F, GLY240D, HIS145D, HIS157D, HIS162D, HIS162F, HIS243D, ILE112F, ILE131F, ILE150D, ILE210F, ILE249D, LEU104F, LEU105F, LEU133F, LEU164D, LEU164F, LEU173D, LEU174D, LEU185F, LEU194F, LEU206F, LEU215F, LEU216F, LEU225F, LEU229F, LEU237D, LEU239D, LEU241D, LEU246D, LEU251D, LYS213F, LYS223F, MET128F, MET233D, PHE146D, PHE172D, PRO110F, PRO126F, PRO147D, PRO152D, PRO161D, PRO214F, SER129F, SER148D, SER166D, SER238D, THR175D, THR183F, THR226F, TRP160D, TRP160F, TYR171D, TYR187D, TYR187F, TYR220F, VAL109F, VAL111F, VAL127F, VAL132F, VAL151D, VAL158D, VAL159F, VAL163F, VAL190F, VAL193F, VAL224F, VAL232D, VAL63F
• E-F: ALA113E, ALA156F, ALA184E, ALA189E, ALA211E, ALA217E, ALA221E, ALA235F, ALA242F, ALA245F, ALA247F, ALA248F, ARG168E, ARG168F, ARG170F, ARG182E, ARG202E, ARG218E, ARG219E, ARG222E, ARG227E, ARG230E, ARG231E, ARG231F, ASN167F, ASN191E, ASP130E, ASP142F, ASP154F, ASP186E, ASP186F, ASP236F, ASP250F, GLN177F, GLN228E, GLN228F, GLU108E, GLU124E, GLU192E, GLU212E, GLU234F, GLU244F, GLY149F, GLY153F, GLY155F, GLY165E, GLY165F, GLY169F, GLY176F, GLY188E, GLY209E, GLY240F, HIS145F, HIS157F, HIS162E, HIS162F, HIS243F, ILE112E, ILE131E, ILE150F, ILE210E, ILE249F, LEU104E, LEU105E, LEU133E, LEU164E, LEU164F, LEU173F, LEU174F, LEU185E, LEU194E, LEU206E, LEU215E, LEU216E, LEU225E, LEU229E, LEU237F, LEU239F, LEU241F, LEU246F, LEU251F, LYS213E, LYS223E, MET128E, MET233F, PHE146F, PHE172F, PRO110E, PRO126E, PRO147F, PRO152F, PRO161F, PRO214E, SER129E, SER148F, SER166F, SER238F, THR175F, THR183E, THR226E, TRP160E, TRP160F, TYR171F, TYR187E, TYR187F, TYR220E, VAL109E, VAL111E, VAL127E, VAL132E, VAL151F, VAL158F, VAL159E, VAL163E, VAL190E, VAL224E, VAL232E, VAL232F, VAL63E
• E-G: LEU4E, LEU4G
• E-J: ARG22E, ASN17J, GLN13E, GLN13J, GLN9E, GLU10E, GLU16J, GLU21E, GLU34J, GLU47J, GLY35J, HIS32J, ILE18J, LYS14J, PHE8E, PRO7E, SER12E, THR33J, THR43J, TYR15J
• G-H: ALA113H, ALA156G, ALA184H, ALA189H, ALA211H, ALA217H, ALA221H, ALA235G, ALA242G, ALA245G, ALA247G, ALA248G, ARG168G, ARG168H, ARG170G, ARG182H, ARG202H, ARG218H, ARG219H, ARG222H, ARG227H, ARG230H, ARG231G, ARG231H, ASN167G, ASN191H, ASP130H, ASP154G, ASP186G, ASP186H, ASP236G, ASP250G, GLN177G, GLN228G, GLN228H, GLU108H, GLU124H, GLU192H, GLU212H, GLU234G, GLU244G, GLY149G, GLY153G, GLY155G, GLY165G, GLY165H, GLY169G, GLY176G, GLY188H, GLY209H, GLY240G, HIS145G, HIS157G, HIS162G, HIS162H, HIS243G, ILE112H, ILE131H, ILE150G, ILE210H, ILE249G, LEU104H, LEU105H, LEU133H, LEU164G, LEU164H, LEU173G, LEU174G, LEU185H, LEU194H, LEU206H, LEU215H, LEU216H, LEU225H, LEU229G, LEU229H, LEU237G, LEU239G, LEU241G, LEU246G, LEU251G, LYS213H, LYS223H, MET128H, MET233G, PHE146G, PHE172G, PRO110H, PRO126H, PRO147G, PRO152G, PRO161G, PRO214H, SER129H, SER148G, SER166G, THR175G, THR183H, THR226H, TRP160G, TRP160H, TYR171G, TYR187G, TYR187H, TYR220H, VAL109H, VAL111H, VAL127H, VAL132H, VAL151G, VAL158G, VAL159G, VAL159H, VAL163G, VAL163H, VAL190H, VAL224H, VAL232G, VAL232H, VAL63H
• G-I: ALA156I, ALA184G, ALA189G, ALA211G, ALA217G, ALA221G, ALA235I, ALA242I, ALA245I, ALA247I, ALA248I, ARG168G, ARG168I, ARG170I, ARG182G, ARG202G, ARG218G, ARG219G, ARG222G, ARG227G, ARG230G, ARG231G, ARG231I, ASN167I, ASN191G, ASP130G, ASP142I, ASP154I, ASP186G, ASP186I, ASP236I, ASP250I, GLN177I, GLN228G, GLU108G, GLU192G, GLU212G, GLU234I, GLU244I, GLY149I, GLY153I, GLY155I, GLY165G, GLY165I, GLY169I, GLY176I, GLY188G, GLY209G, GLY240I, HIS145I, HIS157I, HIS162G, HIS162I, HIS243I, ILE112G, ILE131G, ILE150I, ILE210G, ILE249I, LEU104G, LEU105G, LEU133G, LEU164G, LEU164I, LEU173I, LEU174I, LEU185G, LEU194G, LEU206G, LEU215G, LEU216G, LEU225G, LEU229G, LEU237I, LEU239I, LEU241I, LEU246I, LEU251I, LYS213G, LYS223G, MET128G, MET233I, PHE146I, PHE172I, PRO110G, PRO126G, PRO147I, PRO152I, PRO161I, PRO214G, SER129G, SER148I, SER166I, THR175I, THR183G, THR226G, TRP160I, TYR171I, TYR187G, TYR187I, TYR220G, VAL109G, VAL111G, VAL127G, VAL132G, VAL151I, VAL158I, VAL159G, VAL163G, VAL190G, VAL224G, VAL232I, VAL63G
• G-J: ALA235G, ALA235J, ALA242G, ALA242J, ALA245G, ALA245J, ALA44G, ALA44J, ALA49G, ALA49J, ALA56G, ALA56J, ALA5G, ALA5J, ARG219G, ARG219J, ARG227G, ARG227J, ARG230G, ARG230J, ARG231G, ARG231J, ARG59G, ARG59J, ARG99G, ARG99J, ASN102G, ASN102J, ASN103G, ASN103J, ASN17G, ASN17J, ASP236G, ASP236J, ASP46G, ASP46J, ASP54G, ASP54J, ASP58G, ASP58J, ASP89G, ASP89J, ASP91G, ASP91J, CYS51G, CYS51J, CYS57G, CYS57J, GLN228G, GLN228J, GLN98G, GLN98J, GLU108G, GLU108J, GLU16G, GLU16J, GLU234G, GLU234J, GLU47G, GLU47J, GLU92G, GLU92J, GLU96G, GLU96J, GLY240G, GLY240J, HIS45G, HIS45J, HIS53G, HIS53J, HIS88G, HIS88J, ILE107G, ILE107J, ILE18G, ILE18J, ILE249G, ILE249J, ILE55G, ILE55J, ILE93G, ILE93J, ILE94G, ILE94J, LEU105G, LEU105J, LEU215G, LEU215J, LEU216G, LEU216J, LEU229G, LEU229J, LEU237G, LEU237J, LEU239G, LEU239J, LEU241G, LEU241J, LEU246G, LEU48G, LEU48J, LEU4G, LEU4J, LYS14G, LYS14J, LYS223G, LYS223J, MET233G, MET233J, PHE95G, PHE95J, PRO87G, PRO87J, SER106G, SER106J, SER238G, SER238J, SER42G, SER42J, THR226G, THR226J, THR43G, THR43J, THR6G, THR6J, THR86G, THR86J, TRP90G, TRP90J, TYR15G, TYR15J, TYR220G, TYR220J, TYR50G, TYR50J, VAL109G, VAL109J, VAL224G, VAL224J, VAL232G, VAL232J
• G-K: ALA235G, ALA235K, ALA242G, ALA242K, ALA245G, ALA245K, ALA247G, ALA247K, ALA248G, ALA248K, ASP236G, ASP236K, ASP250G, ASP250K, ASP89G, ASP89K, ASP91G, ASP91K, GLN98G, GLN98K, GLU234G, GLU234K, GLU244G, GLU244K, GLY240G, GLY240K, GLY252G, GLY252K, GLY85G, GLY85K, HIS157G, HIS243G, HIS243K, HIS88G, HIS88K, ILE249G, ILE249K, ILE94G, ILE94K, LEU237G, LEU237K, LEU239G, LEU239K, LEU241G, LEU241K, LEU246G, LEU246K, LEU251G, LEU251K, PHE95G, PHE95K, PRO87G, PRO87K, SER238G, SER238K, THR86G, THR86K, TRP90G, TRP90K
• G-L: ARG227G, ARG227L, ARG231G, ARG231L
• H-I: ALA156H, ALA184I, ALA189I, ALA211I, ALA217I, ALA221I, ALA235H, ALA242H, ALA245H, ALA247H, ALA248H, ARG168H, ARG168I, ARG170H, ARG182I, ARG202I, ARG218I, ARG219I, ARG222I, ARG227I, ARG230I, ARG231H, ARG231I, ASN167H, ASN191I, ASP130I, ASP154H, ASP186H, ASP186I, ASP236H, ASP250H, GLN177H, GLN228I, GLU108I, GLU192I, GLU212I, GLU234H, GLU244H, GLY149H, GLY153H, GLY155H, GLY165H, GLY165I, GLY169H, GLY176H, GLY188I, GLY209I, GLY240H, HIS145H, HIS157H, HIS162H, HIS162I, HIS243H, ILE112I, ILE131I, ILE150H, ILE210I, ILE249H, LEU104I, LEU105I, LEU133I, LEU164H, LEU164I, LEU173H, LEU174H, LEU185I, LEU194I, LEU206I, LEU215I, LEU216I, LEU225I, LEU229H, LEU229I, LEU237H, LEU239H, LEU241H, LEU246H, LEU251H, LYS213I, LYS223I, MET128I, MET233H, PHE146H, PHE172H, PRO110I, PRO126I, PRO147H, PRO152H, PRO161H, PRO214I, SER129I, SER148H, SER166H, SER238H, THR175H, THR183I, THR226I, TRP160H, TRP160I, TYR171H, TYR187H, TYR187I, TYR220I, VAL109I, VAL111I, VAL127I, VAL132I, VAL151H, VAL158H, VAL159H, VAL159I, VAL163H, VAL163I, VAL190I, VAL224I, VAL232H, VAL63I
• H-J: ARG227H, ARG227J, ARG231H, ARG231J
• H-K: ALA235H, ALA235K, ALA242H, ALA242K, ALA245H, ALA245K, ALA44K, ALA49H, ALA49K, ALA56H, ALA56K, ALA5H, ALA5K, ARG219H, ARG219K, ARG227H, ARG227K, ARG230H, ARG230K, ARG231H, ARG231K, ARG59H, ARG59K, ARG99H, ARG99K, ASN102H, ASN102K, ASN103H, ASN103K, ASP236H, ASP236K, ASP46H, ASP46K, ASP54H, ASP54K, ASP58H, ASP58K, ASP89H, ASP89K, ASP91H, ASP91K, CYS51H, CYS51K, CYS57H, CYS57K, GLN228H, GLN228K, GLN98H, GLN98K, GLU108H, GLU108K, GLU10H, GLU10K, GLU16K, GLU234H, GLU234K, GLU47H, GLU47K, GLU92H, GLU92K, GLU96H, GLU96K, GLY240H, GLY240K, HIS45K, HIS53H, HIS53K, HIS88H, HIS88K, ILE107H, ILE107K, ILE18K, ILE249H, ILE249K, ILE55H, ILE55K, ILE93H, ILE93K, ILE94H, ILE94K, LEU105H, LEU105K, LEU215H, LEU215K, LEU216H, LEU216K, LEU229H, LEU229K, LEU237H, LEU237K, LEU239H, LEU239K, LEU241H, LEU241K, LEU246H, LEU246K, LEU48K, LEU4H, LEU4K, LYS14H, LYS14K, LYS223H, LYS223K, MET233H, MET233K, PHE52H, PHE95H, PHE95K, PRO87H, PRO87K, SER106H, SER106K, SER238H, SER238K, SER42K, THR226H, THR226K, THR43H, THR43K, THR6H, THR6K, THR86H, THR86K, TRP90H, TRP90K, TYR15H, TYR15K, TYR220H, TYR220K, TYR50H, TYR50K, VAL109H, VAL109K, VAL224H, VAL224K, VAL232H, VAL232K
• H-L: ALA235H, ALA235L, ALA242H, ALA242L, ALA245H, ALA245L, ALA247H, ALA247L, ALA248H, ALA248L, ASP236H, ASP236L, ASP250H, ASP250L, ASP89H, ASP89L, ASP91H, ASP91L, GLN98H, GLN98L, GLU234H, GLU234L, GLU244H, GLU244L, GLY240H, GLY240L, GLY252L, GLY85H, GLY85L, HIS243H, HIS243L, HIS88H, HIS88L, ILE249H, ILE249L, ILE94H, ILE94L, LEU237H, LEU237L, LEU239H, LEU239L, LEU241H, LEU241L, LEU246H, LEU246L, LEU251H, LEU251L, PHE95L, PRO87H, PRO87L, SER238H, SER238L, THR86H, THR86L, TRP90H, TRP90L
• I-J: ALA235I, ALA235J, ALA242I, ALA242J, ALA245I, ALA245J, ALA247I, ALA247J, ALA248I, ALA248J, ASP236I, ASP236J, ASP250I, ASP250J, ASP89I, ASP89J, ASP91I, ASP91J, GLN98I, GLN98J, GLU234I, GLU234J, GLU244I, GLU244J, GLY240I, GLY240J, GLY252I, GLY252J, GLY85I, GLY85J, HIS243I, HIS243J, HIS88I, HIS88J, ILE249I, ILE249J, ILE94I, ILE94J, LEU237I, LEU237J, LEU239I, LEU239J, LEU241I, LEU241J, LEU246I, LEU246J, LEU251I, LEU251J, PHE95I, PHE95J, PRO87I, PRO87J, SER238I, SER238J, THR86I, THR86J, TRP90I, TRP90J
• I-K: ARG227I, ARG227K, ARG231I, ARG231K
• I-L: ALA235I, ALA235L, ALA242I, ALA242L, ALA245I, ALA245L, ALA49I, ALA49L, ALA56I, ALA56L, ALA5I, ALA5L, ARG219I, ARG219L, ARG227I, ARG227L, ARG230I, ARG230L, ARG231I, ARG231L, ARG59I, ARG59L, ARG99I, ARG99L, ASN102I, ASN102L, ASN103I, ASN103L, ASP236I, ASP236L, ASP46I, ASP46L, ASP54I, ASP54L, ASP58I, ASP58L, ASP89I, ASP89L, ASP91I, ASP91L, CYS57I, CYS57L, GLN228L, GLN98I, GLN98L, GLU108I, GLU108L, GLU234I, GLU234L, GLU47L, GLU92I, GLU92L, GLU96I, GLU96L, GLY240I, GLY240L, GLY97L, HIS53I, HIS53L, HIS88I, HIS88L, ILE107I, ILE107L, ILE249I, ILE249L, ILE55I, ILE55L, ILE93I, ILE93L, ILE94I, ILE94L, LEU105I, LEU105L, LEU215I, LEU215L, LEU216I, LEU216L, LEU229I, LEU229L, LEU237I, LEU237L, LEU239I, LEU239L, LEU241I, LEU241L, LEU246I, LEU246L, LEU4I, LYS14I, LYS14L, LYS223I, LYS223L, MET233I, MET233L, PHE95I, PHE95L, PRO87I, PRO87L, SER106I, SER106L, SER238I, SER238L, THR226I, THR226L, THR6I, THR6L, THR86I, THR86L, TRP90I, TRP90L, TYR15I, TYR15L, TYR220I, TYR220L, TYR50I, TYR50L, VAL109I, VAL109L, VAL224I, VAL224L, VAL232I, VAL232L
• J-K: ALA113J, ALA156K, ALA184J, ALA189J, ALA211J, ALA217J, ALA221J, ALA235K, ALA242K, ALA245K, ALA247K, ALA248K, ARG168J, ARG168K, ARG170K, ARG182J, ARG202J, ARG218J, ARG219J, ARG222J, ARG227J, ARG230J, ARG231J, ARG231K, ASN167K, ASN191J, ASP130J, ASP142K, ASP154K, ASP186J, ASP186K, ASP236K, ASP250K, GLN177K, GLN228J, GLN228K, GLU108J, GLU192J, GLU212J, GLU234K, GLU244K, GLY149K, GLY153K, GLY155K, GLY165J, GLY165K, GLY169K, GLY176K, GLY188J, GLY209J, GLY240K, HIS145K, HIS157K, HIS162J, HIS162K, HIS243K, ILE112J, ILE131J, ILE150K, ILE210J, ILE249K, LEU104J, LEU105J, LEU133J, LEU164J, LEU164K, LEU173K, LEU174K, LEU185J, LEU194J, LEU206J, LEU215J, LEU216J, LEU225J, LEU229J, LEU237K, LEU239K, LEU241K, LEU246K, LEU251K, LYS213J, LYS223J, MET128J, MET233K, PHE146K, PHE172K, PRO110J, PRO126J, PRO147K, PRO152K, PRO161K, PRO214J, SER129J, SER148K, SER166K, SER238K, THR175K, THR183J, THR226J, TRP160J, TRP160K, TYR171K, TYR187J, TYR187K, TYR220J, VAL109J, VAL111J, VAL127J, VAL132J, VAL151K, VAL158K, VAL159J, VAL163J, VAL190J, VAL193J, VAL224J, VAL232J, VAL232K, VAL63J
• J-L: ALA113L, ALA156J, ALA184L, ALA189L, ALA211L, ALA217L, ALA221L, ALA235J, ALA242J, ALA245J, ALA247J, ALA248J, ARG168J, ARG168L, ARG170J, ARG182L, ARG202L, ARG218L, ARG219L, ARG222L, ARG227L, ARG230L, ARG231J, ARG231L, ASN167J, ASN191L, ASP130L, ASP142J, ASP154J, ASP186J, ASP186L, ASP236J, ASP250J, GLN177J, GLN228J, GLN228L, GLU108L, GLU192L, GLU212L, GLU234J, GLU244J, GLY149J, GLY153J, GLY155J, GLY165J, GLY165L, GLY169J, GLY176J, GLY188L, GLY209L, GLY240J, HIS145J, HIS157J, HIS162J, HIS162L, HIS243J, ILE112L, ILE131L, ILE150J, ILE210L, ILE249J, LEU104L, LEU105L, LEU133L, LEU164J, LEU164L, LEU173J, LEU174J, LEU185L, LEU194L, LEU206L, LEU215L, LEU216L, LEU225L, LEU229J, LEU229L, LEU237J, LEU239J, LEU241J, LEU246J, LEU251J, LYS213L, LYS223L, MET128L, MET233J, PHE146J, PHE172J, PRO110L, PRO126L, PRO147J, PRO152J, PRO161J, PRO214L, SER129L, SER148J, SER166J, SER238J, THR175J, THR183L, THR226L, TRP160J, TRP160L, TYR171J, TYR187J, TYR187L, TYR220L, VAL109L, VAL111L, VAL127L, VAL132L, VAL151J, VAL158J, VAL159J, VAL159L, VAL163J, VAL163L, VAL190L, VAL193L, VAL224L, VAL232J, VAL232L, VAL63L
• K-L: ALA113K, ALA156L, ALA184K, ALA189K, ALA211K, ALA217K, ALA221K, ALA235L, ALA242L, ALA245L, ALA247L, ALA248L, ARG168K, ARG168L, ARG170L, ARG182K, ARG202K, ARG218K, ARG219K, ARG222K, ARG227K, ARG230K, ARG231K, ARG231L, ASN167L, ASN191K, ASP130K, ASP142L, ASP154L, ASP186K, ASP186L, ASP236L, ASP250L, GLN177L, GLN228K, GLN228L, GLU108K, GLU192K, GLU212K, GLU234L, GLU244L, GLY149L, GLY153L, GLY155L, GLY165K, GLY165L, GLY169L, GLY176L, GLY188K, GLY209K, GLY240L, HIS145L, HIS157L, HIS162K, HIS162L, HIS243L, ILE112K, ILE131K, ILE150L, ILE210K, ILE249L, LEU104K, LEU105K, LEU133K, LEU164K, LEU164L, LEU173L, LEU174L, LEU185K, LEU194K, LEU206K, LEU215K, LEU216K, LEU225K, LEU229K, LEU237L, LEU239L, LEU241L, LEU246L, LEU251L, LYS213K, LYS223K, MET128K, MET233L, PHE146L, PHE172L, PRO110K, PRO126K, PRO147L, PRO152L, PRO161L, PRO214K, SER129K, SER148L, SER166L, SER238L, THR175L, THR183K, THR226K, TRP160K, TRP160L, TYR171L, TYR187K, TYR187L, TYR220K, VAL109K, VAL111K, VAL127K, VAL132K, VAL151L, VAL158L, VAL159K, VAL163K, VAL190K, VAL224K, VAL232L, VAL63K