1RWB

cooperative effect of two surface amino acid mutations (q252l and e170k) of glucose dehydrogenase from bacillus megaterium iwg3 for the stabilization of oligomeric state

• Total interactions analyzed: 6
• Total true interactions: 4
• Strongest Interaction:
  • Chains: E-F
  • Int. Res.: 153
  • Norm. En. per Res.: -4.0672
• Hub Node: B(2)

All Interactions

Chains	Hydro. Bond Ener. (kJ/mol)	Elec. Ener. (kJ/mol)	VDW. Ener. (kJ/mol)	Tot. Stab. Ener. (kJ/mol)	#int. res.	# Short cont.	#Hydr. int.	#VDW pairs	#salt bridges	#Pot. fav. elec. int	#Pot. unfav. elec. int
A-B	-39.9984	-17.982	-560.985	-618.9654	154	28	10	21747	0	40	45
B-E	-6.017	-16.7767	-40.177	-62.9708	41	0	0	2284	2	20	20
B-F	0.0	7.3448	-3.7571	3.5877	21	0	0	411	1	14	20
E-F	-40.0126	-20.6473	-561.626	-622.286	153	26	11	21544	0	40	45

Interface residues

• A-B: ALA120A, ALA120B, ALA127A, ALA127B, ALA159A, ALA159B, ALA160A, ALA160B, ALA173A, ALA173B, ALA177A, ALA177B, ARG125A, ARG125B, ASN110A, ASN110B, ASN116A, ASN116B, ASP108A, ASP108B, ASP114A, ASP114B, GLU103A, GLU103B, GLU126A, GLU126B, GLU133A, GLU133B, GLU148A, GLU148B, GLU175A, GLU175B, GLU69A, GLU69B, GLY119A, GLY119B, GLY163A, GLY163B, GLY164A, GLY164B, GLY241A, GLY241B, HIS102A, HIS102B, HIS147A, HIS147B, HIS157A, HIS157B, ILE113A, ILE113B, ILE128A, ILE128B, ILE150A, ILE150B, ILE181A, ILE181B, ILE73A, ILE73B, LEU106A, LEU106B, LEU117A, LEU117B, LEU122A, LEU122B, LEU154A, LEU154B, LEU167A, LEU167B, LEU172A, LEU172B, LEU174A, LEU174B, LYS129A, LYS129B, LYS149A, LYS149B, LYS162A, LYS162B, LYS166A, LYS166B, LYS170A, LYS170B, LYS179A, LYS179B, MET104A, MET104B, MET165A, MET165B, MET168A, MET168B, PHE121A, PHE121B, PHE131A, PHE131B, PHE155A, PHE155B, PRO151A, PRO151B, PRO153A, PRO153B, PRO178A, PRO178B, SER100A, SER100B, SER101A, SER101B, SER105A, SER105B, SER107A, SER107B, SER124A, SER124B, SER144B, SER145A, SER145B, SER161A, SER161B, SER70A, SER70B, THR118A, THR118B, THR169A, THR169B, THR171A, THR171B, THR67A, THR67B, TRP109A, TRP109B, TRP152A, TRP152B, TYR130A, TYR130B, TYR158A, TYR158B, TYR176A, TYR176B, TYR253A, TYR253B, VAL132A, VAL132B, VAL146A, VAL146B, VAL156A, VAL156B, VAL183A, VAL66A, VAL66B, VAL68A, VAL68B, VAL72A, VAL72B, VAL99A, VAL99B
• B-E: ASN134B, ASN97E, ASP108E, ASP135B, ASP4B, ASP87B, GLN205E, GLU103E, GLU133B, GLU198E, GLU6B, GLU82B, GLY7B, GLY84B, HIS102E, ILE136B, ILE195E, ILE80B, LEU106E, LEU154E, LEU5B, LEU86B, LYS111E, LYS137B, LYS199E, LYS33B, LYS81B, LYS85B, LYS8B, MET104E, PHE131B, PHE83B, PRO98E, SER100E, SER105E, SER107E, TRP109E, TYR130B, VAL132B, VAL99E, VAL9B
• B-F: ARG125F, ASN134B, ASN134F, ASP135B, ASP135F, ASP4B, GLN77F, GLU133F, GLU69F, GLU6B, ILE136B, ILE73F, LYS129F, LYS137B, LYS179F, LYS31B, LYS3B, LYS8B, SER234B, SER70F, VAL132F
• E-F: ALA120E, ALA120F, ALA127E, ALA127F, ALA159E, ALA159F, ALA160E, ALA160F, ALA173E, ALA173F, ALA177E, ALA177F, ARG125E, ARG125F, ASN110E, ASN110F, ASN116E, ASN116F, ASP108E, ASP108F, ASP114E, ASP114F, GLU103E, GLU103F, GLU126E, GLU126F, GLU133E, GLU133F, GLU148E, GLU148F, GLU175E, GLU175F, GLU69E, GLU69F, GLY119E, GLY119F, GLY163E, GLY163F, GLY164E, GLY164F, GLY241E, GLY241F, HIS102E, HIS102F, HIS147E, HIS147F, HIS157E, HIS157F, ILE113E, ILE113F, ILE128E, ILE128F, ILE150E, ILE150F, ILE181E, ILE181F, ILE73E, ILE73F, LEU106E, LEU106F, LEU117E, LEU117F, LEU122E, LEU122F, LEU154E, LEU154F, LEU167E, LEU167F, LEU172E, LEU172F, LEU174E, LEU174F, LYS129E, LYS129F, LYS149E, LYS149F, LYS162E, LYS162F, LYS166E, LYS166F, LYS170E, LYS170F, LYS179E, LYS179F, MET104E, MET104F, MET165E, MET165F, MET168E, MET168F, PHE121E, PHE121F, PHE131E, PHE131F, PHE155E, PHE155F, PRO151E, PRO151F, PRO153E, PRO153F, PRO178E, PRO178F, SER100E, SER100F, SER101E, SER101F, SER105E, SER105F, SER107E, SER107F, SER124E, SER124F, SER145F, SER161E, SER161F, SER70E, SER70F, THR118E, THR118F, THR169E, THR169F, THR171E, THR171F, THR67E, THR67F, TRP109E, TRP109F, TRP152E, TRP152F, TYR130E, TYR130F, TYR158E, TYR158F, TYR176E, TYR176F, TYR253E, TYR253F, VAL132E, VAL132F, VAL146E, VAL146F, VAL156E, VAL156F, VAL183E, VAL183F, VAL66E, VAL66F, VAL68E, VAL68F, VAL72E, VAL72F, VAL99E, VAL99F