1. HORI-Server Introduction:
HORI is a web server to compute higher order residue interactions in protein structures. HORI offers a set of programs to calculate Pairwise, Triplet and Quadruplet interactions with in the protein structures.
Figure 1 : Graphical representation of individual Higher order residue interactions mapped on to Crambin (PDB ID: 1CRN)
HORI Server is designed as three modules :
- HORI-Global : Complete structure level computation of different higher order residue interactions
- HORI-Lite : Specific higher order residue interaction computation based on residue numbers in input PDB files
- HORI-Cluster : Higher order residue interaction computation based on residue types in input PDB files
HORI-Global offers 2 programs for computationaly intensive calculation of Pairwise, Triplet and Quadruplet interactions in the protein structures. HORI-Lite and HORI-Clusters offers a set of 9 programs for comparatively quick calculations of specific/generic interactions with in protein structures. HORI-Global, HORI-Lite and HORI-Cluster computations can be performed using C-α or C-β based distance calculation methods. Easy-to-use web interfaces are available for all programs in HORI server to calculate using single chain and multichain proteins (2 chains at a time).
HORI server requires a PDB file as its input for the computation of higher order residue interactions. HORI Server provides separate interface for single chain and multi chain proteins. If you would like to submit an NMR structure , Please submit Chain IDs of two chains at a time in multi-chain based programs. Due to computational intense nature of HORI calculations, currently the server will accept only two chain for calculation. Users may remove HETATM / ANISOU records from the PDB Files.